Anti-Fibronectin antibody [N1N2], N-term GTX112794 from GeneTex

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Nature communications
January 19 2017

Fig 1: Glucocorticoid receptor activates YAP by inducing FN1 expression.(a,b) Quantification of cells with nuclear YAP (a) and representative immunofluorescence (b) of serum-starved MDA-MB-231 treated with 1 μM betamethasone (BM) for the indicated times. (c) Quantitative PCR with reverse transcription (qRT–PCR) analysis of serum-starved MDA-MB-231 treated with 1 μM BM for the indicated times. Left y axis is relative to GILZ, right y axis is relative to ANKRD1. (d) qRT–PCR analysis of serum-starved MDA-MB-231 treated with 1 μM BM alone or in combination with RU486 1 μM for 24 h. Error bars represent mean±s.d., from n=3 biological replicates. (e) MDA-MB-231 cells were treated with BM 1 μM alone or in combination with RU486 1 μM for 24 h. Representative blots are shown. Experiment repeated three times. (f) MDA-MB-231 cells were serum starved and treated with BM 1 μM for 24 h. Extracellular fibronectin was stained by immunofluorescence. Experiment repeated three times. Scale bars, 15 μm. (g) Bright-field microscopy image of MDA-MB-231 cells treated with BM 1 μM alone or in combination with RU486 1 μM for 24 h. Experiment repeated three times. Scale bars, 15 μm. (h) MDA-MB-231 were serum starved and treated with BM 1 μM alone or in combination with RU486 1 μM for 6 h. Representative blots are shown. pYAP is phospho-Ser127. Experiment repeated three times. (i,j) Quantification (i) and representative images (j) of MDA-MB-231 cells with nuclear YAP by immunofluorescence. Cells were grown in serum-free medium and treated with BM 1 μM alone or in combination with RGD 500 μg ml−1 for 6 h. Scale bars, 15 μm. Error bars represent mean±s.d., from n=3 biological replicates. *P<0.05, **P<0.01; two-tailed Student's t-test is used throughout.
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EMBO reports
February 05 2020

Fig 2: ISX expression induces TWIST1 and Snail1 expression, and promotes EMT AThe expression levels of ISX, TWIST1, Snail1, fibronectin (FN1), VEGF, and E-cadherin (CDH1) mRNA were examined in a ISX-GFP inducible Tet-ON transformants at 8 h after addition of DOX. Data are presented as mean ± SD in graph (P < 0.001 compared with time point 0 h; Student's t-test) of three independent experiments, each performed in triplicate.BWestern blotting analysis of the protein levels of ISX, HIF1a, TWIST1, Snail1, Slug, ZEB1, Bmi1, E-cadherin (E-Cad.), fibronectin, N-cadherin (N-Cad.), vimentin, and VEGF in A549 and H1299 cells with DOX-inducible ISX expression system 8 h after DOX induction.C, DISX transcriptionally activates luciferase activity driven by TWIST1 (C) and Snail1 (D) promoter regions in A549 cells. Data are presented as mean ± SD in bar graph (P < 0.001, Student's t-test) of three independent experiments, each performed in triplicate.EChIP analysis of ISX binding to the TWIST1 promoter region in A549 and H1299 cells (10% input of each group was pull down and applied to qPCR). Data are presented as mean ± SD in bar graph (P < 0.001, Student's t-test) of three independent experiments, each performed in triplicate.FISX transactivation activity analyzed by luciferase activity driven by the TWIST1 promoter. Data are presented as mean ± SD in graph (P < 0.001, Student's t-test) of three independent experiments, each performed in triplicate.GChIP analysis of ISX binding to the endogenous promoters of TWIST1 (-180/+35) and Snail1(-160/+40) in A549 and cells (10% input of each group was pull down and applied to qPCR). Data are presented as mean ± SD in bar graph (P < 0.001, Student's t-test) of three independent experiments, each performed in triplicate.HEffect of forced expression and knockdown of ISX on cell migration (wound healing) measured in A549 cells. ISXi, ISX-specific shRNAi (described in Materials and Methods). Data are presented as mean ± SD in graph (**P < 0.01, ***P < 0.001, Student's t-test) of three independent experiments, each performed in triplicate. Scale bar, 100 µm.IEffect of forced expression and knockdown of ISX on cell invasion (Transwell) activity measured in A549 cells. Data are presented as mean ± SD in bar graph (***P < 0.001, Student's t-test) of three independent experiments, each performed in triplicate. Scale bar, 100 µm. Source data are available online for this figure.
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Cell death & disease
August 26 2022

Fig 3: Transcriptional signature in HGPS primary fibroblasts.A Scatterplot of differentially expressed genes (DEGs) of 2YO and 8YO HGPS fibroblasts. Common genes of 2YO and 8YO HGPS are shown in gray, 2YO HGPS unique genes in blue and 8YO HGPS unique genes in red. B Heatmap of DEGs from 2YO and 8YO HGPS vs. age-matched control cells (2YO Ctrl and 8YO Ctrl). C Ingenuity Pathway Analysis (IPA) showing enriched categories of common DEGs in 2YO and 8YO HGPS fibroblasts. D qRT-PCR of genes associated with fibrosis: Actb (actin beta), Actg1 (actin gamma 1), Col1A1 (Collagen Type I Alpha 1 Chain), Ctgf (Connective tissue growth factor), Dusp1 (Dual Specificity Phosphatase 1), Fn1 (Fibronectin1), Myl9 (Myosin Light Chain9), SerpinE1, Thbs1 (Thrombospondin 1), Tpm1 (Tropomyosin 1), Tpm2 (Tropomyosin 2) in 2YO and 8YO HGPS cells compared to 8YO control cells. E Cropped immunoblot for Collagen3A1 (COL3A1) and GAPDH in 8YO control (Ctrl) and 2YO and 8YO HGPS. Plot represents COL3A1/GAPDH ratio based on the average for each experimental point, (n = 3). F Cropped immunoblot for Fibronectin1 (FN1) and GAPDH in 8YO control (Ctrl) and 2YO and 8YO HGPS. Plot represents FN1/GAPDH ratio based on the average for each experimental point, (n = 3).
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Cell death & disease
August 26 2022

Fig 4: TM5441 treatment restores normal nuclear shape and reduces the accumulation of fibrosis.A–D qRT-PCR for Col1A1 (n = 3), Fn1 (n = 4), Thbs1 (n = 4) and Actb (n = 3) in control (Ctrl) and 8YO HGPS untreated and treated with TM5441 (TM). E Cropped immunoblot for Collagen3A1 (COL3A1) and GAPDH in 8YO HGPS untreated and treated with TM5441(TM) and age-matched control (Ctrl) cells. Plot represents COL3A1/GAPDH ratio based on the average for each experimental point, (n = 3). F Representative images of immunofluorescence for DAPI (blue) in 8YO HGPS fibroblasts untreated (NT) or treated with TM5441 (TM) and age-matched control (Ctrl). Scale bar 30 μM. Quantification of regular and abnormal nuclei in control (Ctrl) and 8YO HGPS cells untreated (NT) or treated with TM5441 (TM), (n = 3). G Quantification of nuclear circularity in control (Ctrl) and 8Y HGPS cells untreated (NT) or treated with TM5441 (TM).

Description

GeneTex's Anti-Fibronectin antibody [N1N2], N-term is a Rabbit Polyclonal antibody. The Anti-Fibronectin antibody [N1N2], N-term was generated using fibronectin 1, CIG, ED-B, FINC, FN, FNZ, GFND, GFND2, LETS, MSF, SMDCF as the antigen. The Anti-Fibronectin antibody [N1N2], N-term from GeneTex is a Rabbit Polyclonal antibody to Fibronectin. The Anti-Fibronectin antibody [N1N2], N-term Recognizes Human, Mouse, Rat. The Anti-Fibronectin antibody [N1N2], N-term has been validated for the following Applications: WB, ICC/IF, IHC-P, IHC-Fr, IP, ELISA

Product Specs
ItemAnti-Fibronectin antibody [N1N2], N-term
CompanyGeneTex
Price Pricing Info Supplier Page
Catalog NumberGTX112794
Quantity100 µl
ApplicationsWB, ICC/IF, IHC-P, IHC-Fr, IP, ELISA
Concentration0.48mg/ml
Conjugate/TagUnconjugated
PurityPurified by antigen-affinity chromatography
ReactivityHuman, Mouse, Rat
TargetFibronectin
IsotypeIgG
HostRabbit
Antibody TypePrimary Antibodies
ImmunogenRecombinant protein encompassing a sequence within the N-terminus region of human Fibronectin. The exact sequence is proprietary
NCBI Full Gene Namefibronectin 1
NCBI Gene AliasesED-B, fibronectin 1, CIG, ED-B, FINC, FN, FNZ, GFND, GFND2, LETS, MSF, SMDCF, CIG, GFND, FNZ, LETS, GFND2, MSF, FINC, FN, SMDCF
ClonalityPolyclonal
FormatPBS, 1% BSA, 20% Glycerol, 0.025% ProClin 300
Research AreaCancer
Gene NameFN1
Molecular Weight263

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Website: http://www.genetex.com

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(2) Phosphodiesterase type 10A inhibitor attenuates lung fibrosis by targeting myofibroblast activationiScienceMay 19, 2023Ya-Jun Li, Jian-Rong Shi, Shu-Chan Li, Lu-Ming Wang, Rana Dhar, Ning Li, Xin-Wei Cao, Zi-Gang Li, Hui-Fang TangRESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, Rabbit Anti-Fibronectin,Genetex,CAT# GTX112794;RRID:AB_1950298 Rabbit Anti-Collagen I,Genetex,CAT# GTX20292; RRID:AB_384293 Rabbit Anti-a-SMA,Abcam,CAT#

(3) Ovatodiolide inhibits SARS-CoV-2 replication and ameliorates pulmonary fibrosis through suppression of the TGF-β/TβRs signaling pathwayBiomedicine & pharmacotherapy = Biomedecine & pharmacotherapieMay 1, 2023Chiou WC, Huang GJ, Chang TY, Hsia TL, Yu HY, Lo JM, Fu PK, Huang C.[26]. Primary antibodies against COL1A1 (Cat. GTX112731), ɑ-SMA (Cat. GTX100034), fibronectin (Cat. GTX112794), TβRI (Cat. GTX134209), TβRII (Cat. GTX129909), pSMAD2 (Cat. GTX133614), ZEB1 (Cat. GTX105278), NOX4

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(5) Injectable and biofunctionalized fibrin hydrogels co-embedded with stem cells induce hair follicle genesisRegenerative biomaterialsJanuary 1, 2023Chen H, Ma X, Zhang M, Liu Z.Antibody name,Detailed information Nestin,"1:100, ab11306, abcam, UK" Fibronectin,"1:100, GTX112794, GeneTex, USA" BMP6,"1:100, ab155963, abcam, UK" CD31,"1:30, GTX54379, GeneTex, USA" Biotin,"1:100,

(6) Aspirin Inhibits Fibronectin Expression and Reverses Fibronectin-Mediated Cell Invasiveness by Activating Akt Signaling in PreeclampsiaPharmaceuticals (Basel, Switzerland)December 8, 2022Su MT, Tsai CW, Tsai PY, Wang CY, Tsai HL.incubated overnight at 4 °C with the following primary antibodies: Fibronectin (0.14 mg/mL, GeneTex, GTX112794, Hsinchu City, Taiwan), Vimentin (0.32 mg/mL, GeneTex, GTX100619, Hsinchu City, Taiwan), β-catenin (0.1

(7) Adaptive multi-degree-of-freedom in situ bioprinting robot for hair-follicle-inclusive skin repair: A preliminary study conducted in miceBioengineering & translational medicineSeptember 1, 2022Zhao W, Chen H, Zhang Y, Zhou D, Liang L, Liu B, Xu T.Biolegend), anti‐nestin (1:100, ab11306, Abcam), anti‐bmp6 (1:100, ab155963, Abcam), anti‐fibronectin (1:100, GTX112794, GeneTex), CD49f‐biotin (1:150, BioLegend), K1 (1:100, 905,601, BioLegend), anti‐CD31 (1:30, g = GTX54379,

(8) SerpinE1 drives a cell-autonomous pathogenic signaling in Hutchinson-Gilford progeria syndromeCell death & diseaseAugust 26, 2022Catarinella G, Nicoletti C, Bracaglia A, Procopio P, Salvatori I, Taggi M, Valle C, Ferri A, Canipari R, Puri PL, et al.C-19), Ser15 P53 (Cell Signaling, 9284), Collagen3A1 (Santa Cruz, sc-271249), Fibronectin1 (Genetex, GTX112794). GAPDH (Cell Signaling, D16H11 5174 S) and tubulin (Neo Markers, Ab4) were used as protein normalization

(9) Phosphorylation of intestine-specific homeobox by ERK1 modulates oncogenic activity and sorafenib resistanceCancer lettersNovember 1, 2021Wang LT, Liu KY, Chiou SS, Huang SK, Hsu SH, Wang SN.

(10) Effect of Invasion of Borrelia burgdorferi in Normal and Neoplastic Mammary Epithelial CellsAntibiotics (Basel, Switzerland)October 24, 2021Gaur G, Sawant JY, Chavan AS, Khatri VA, Liu YH, Zhang M, Sapi E.incubated for 1 h at 4 °C using the following primary antibodies and dilutions: Fibronectin (1:500, GTX112794, GeneTex, Irvine, CA, USA), E-cadherin (1:3000, GTX100443, GeneTex,), N-cadherin (1:3000, ab18203, Abcam,

(11) Modulation of Inherent Niches in 3D Multicellular MSC Spheroids Reconfigures Metabolism and Enhances Therapeutic PotentialCellsOctober 14, 2021Chen LC, Wang HW, Huang CC.

(12) The extracellular matrix complexity of idiopathic epiretinal membranes and the bilaminar arrangement of the associated internal limiting membrane in the posterior retinaGraefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle OphthalmologieSeptember 1, 2021Altera A, Tosi GM, Regoli M, De Benedetto E, Bertelli E.collagen,Abcam,Ab6588,+ on the vitreal and retinal edges,+ Entactin,R&D Systems,AF2570,+,+ Fibronectin,GeneTex,GTX112794,−,+ Anti-ECM protein (excluding laminin chains) antibodies employed in the study and their reactivity

(13) Transplantation of 3D MSC/HUVEC spheroids with neuroprotective and proangiogenic potentials ameliorates ischemic stroke brain injuryBiomaterialsMay 1, 2021Hsu TW, Lu YJ, Lin YJ, Huang YT, Hsieh LH, Wu BH, Lin YC, Chen LC, Wang HW, Chuang JC, et al.

(14) Tea polyphenol epigallocatechin-3-gallate inhibits cell proliferation in a patient-derived triple-negative breast cancer xenograft mouse model via inhibition of proline-dehydrogenase-induced effectsJournal of food and drug analysisMarch 15, 2021Lee WJ, Cheng TC, Yen Y, Fang CL, Liao YC, Kuo CC, Tu SH, Lin LC, Chang HW, Chen LC, et al.USA), p27 (GT114, GeneTex, California, USA), a-SMA (GTX100034, GeneTex, California, USA), ﬁbronectin (GTX112794, GeneTex, California, USA), E-cadherin (GTX100443, GeneTex, California, USA), N-cadherin (GTX127345,

(15) Overcoming interferon (IFN)-γ resistance ameliorates transforming growth factor (TGF)-β-mediated lung fibroblast-to-myofibroblast transition and bleomycin-induced pulmonary fibrosisBiochemical pharmacologyJanuary 1, 2021Chang CJ, Lin CF, Lee CH, Chuang HC, Shih FC, Wan SW, Tai C, Chen CL.

(16) Chaperonin-Containing TCP-1 Promotes Cancer Chemoresistance and Metastasis through the AKT-GSK3β-β-Catenin and XIAP-Survivin PathwaysCancersDecember 21, 2020Chang YX, Lin YF, Chen CL, Huang MS, Hsiao M, Liang PH.

(17) Bis-Indole-Derived Nuclear Receptor 4A1 (NR4A1, Nur77) Ligands as Inhibitors of EndometriosisEndocrinologyApril 1, 2020Mohankumar K, Li X, Sung N, Cho YJ, Han SJ, Safe S.

(18) Simultaneous inhibition of CXCR1/2, TGF-β, and PD-L1 remodels the tumor and its microenvironment to drive antitumor immunityJournal for immunotherapy of cancerMarch 1, 2020Horn LA, Riskin J, Hempel HA, Fousek K, Lind H, Hamilton DH, McCampbell KK, Maeda DY, Zebala JA, Su Z, et al.ab177037, Abcam, Cambridge, UK), anti-occludin (OCLN; NBP1-77037, Novus Biologicals), anti-fibronectin (GTX112794, GeneTex), and anti-osteopontin (ab8448, Abcam). Image quantification was performed by random sampling

(19) PCAF-mediated acetylation of ISX recruits BRD4 to promote epithelial-mesenchymal transitionEMBO reportsFebruary 5, 2020Wang LT, Liu KY, Jeng WY, Chiang CM, Chai CY, Chiou SS, Huang MS, Yokoyama KK, Wang SN, Huang SK, et al.acetylated lysine (1:500 dilution; #9441S Cell Signaling Technology), fibronectin (1:500 dilution; GTX112794; GeneTex), mCherry (1:500 dilution; GTX128508; GeneTex), Slug (1:1,000 dilution; GTX128796; GeneTex),

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Nature communications

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Cell death & disease

Cell death & disease

Anti-Fibronectin antibody [N1N2], N-term from GeneTex

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GeneTex

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Anti-Fibronectin antibody [N1N2], N-term from GeneTex

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