Fibronectin (IST-9) Antibody sc-59826 from Santa Cruz Biotechnology, Inc.

Product Specs
ItemFibronectin (IST-9) Antibody
CompanySanta Cruz Biotechnology, Inc.
Price $316.00Supplier Page View Company Product Page
Catalog Numbersc-59826
Antibody TypePrimary Antibodies
NCBI Gene AliasesLETS, ED-B, CIG, GFND, FNZ, FN, GFND2, Fn, MSF, FINC, E330027I09, Fn-1, SMDCF
Quantity200 µg/ml
CloneIST-9
Reactivitymouse, rat, human, porcine, canine
ApplicationsWB, IP, IF, IHC(P)
Hostmouse
FormatPurified
Conjugate/TagUnconjugated
ClonalityMonoclonal
NCBI Full Gene Namefibronectin 1
Targetcellular Fibronectin
IsotypeIgG1

Questions and Answers

What species does this fibronectin antibody work with in experiments?

The Fibronectin (IST-9) Antibody is reactive with several species, including Homo sapiens (human), mouse, rat, canine, and porcine.

In what types of applications has this antibody been utilized according to the literature?

This antibody has been shown to work in various applications, including Western blotting, immunofluorescence, immunohistochemistry, and immunoprecipitation.

Are there any specific studies that have utilized this antibody for fibronectin detection?

Yes, the antibody has been cited in multiple studies, including those investigating the role of fibronectin in processes such as endothelial-to-mesenchyme-like transition in trabecular meshwork cells related to glaucoma (), and in the context of pancreatic cancer exosomes promoting pre-metastatic niche formation in the liver ().

How has this antibody been applied in cancer research?

The Fibronectin (IST-9) Antibody has been used to study the role of fibronectin extra domain A in promoting sorafenib resistance in hepatocellular carcinoma cells, highlighting its importance in cancer progression and treatment response ().

Santa Cruz Biotechnology, Inc.

10410 Finnell Street

Dallas, Texas 75220

United States

Phone: 1-800-457-3801
831-457-3801

Company Profile

Website: http://www.scbt.com/

(1) Systematic Comparison of the TGF-β Isoforms in Normal Dermal and Lung Fibroblasts Identifies TGF-β2 and TGF-β3 as Priority Targets in Tissue FibrosisCellsApril 10, 2026Raveen Badyal, Brandon Kohlen, Kevin J. Keen, James V. Dunne, Tillie-Louise Hackettantibodies included Collagen-I (1:1000, Abcam, Cat: ab138492), at 139 kDa, and Fibronectin (1:2000, Cat: sc-59826, Santa Cruz Biotechnology, Dallas, TX, USA), at 220 kDa, to assess ECM production. To investigate myofibroblast

(2) Tissue and Isoform-Specific Effects of Platelet-Derived Growth Factor on Neonatal-Derived Dermal and Fetal-Derived Lung Fibroblast Profibrotic FunctionsCellsApril 1, 2026Brandon Kohlen, Raveen Badyal, Kevin J. Keen, James V. Dunne, Tillie-Louise Hackettcat. ab138492, Abcam, Waltham, MA, USA), anti-fibronectin antibody (1:2000 dilution, clone IST-9, cat. sc-59826, Santa Cruz Biotechnology, Dallas, TX, USA), anti-alpha-smooth muscle actin polyclonal antibody (1:300

(3) Splicing isoforms associated with TGFβ-induced myofibroblast activationBMC Molecular and Cell BiologyMarch 5, 2026Opeoluwa Alli-Oke, Danny Bergeron, Fiona Kessai, Philippe Thibault, Mathieu Durand, Jean-Philippe Brosseauwith primary antibodies [Mouse Anti-SMA (Abnova H00000059-M02), or Mouse Anti-EDA + FN (Santa Cruz, sc-59826)] and secondary antibody [Donkey anti-Mouse AF488 (Jackson ImmunoResearch, 715-545-151)]. According

(4) Basement membrane remodeling with fibroblast activation and cystatin C aggregation in cerebral vessels of hereditary cystatin C amyloid angiopathyActa Neuropathologica CommunicationsJanuary 27, 2026Asbjorg Osk Snorradottir, Hjalti Karl Hafsteinsson, Klara Hansdottir, Saevar Ingthorsson, Sigurdur Runar Gudmundsson, Angelos Skodras, Helgi J. Isaksson, Hakon Hakonarsonat 4 °C FN1,"Cell Signaling, E5H6X","Rabbit, monoclonal",pH 9,1:40,60 min FN1 IST-9,"Santa Cruz, sc-59826",Mouse monoclonal,pH 9,1:30,30 min Vimentin,"Agilent, IR630","Mouse, monoclonal",pH 9,Ready-to-use,30

(5) Distinct transcriptional regulation of the Wnt and TGFβ signaling families associated with wound healing and fibrotic outcomes to lens injuryExperimental Eye ResearchDecember 1, 2025Catherine Lalman, Kylie R. Stabler, Joshua Disatham, Lisa A. Brennan, Marc Kantorow, Janice L. Walkerday 3 for alpha smooth muscle actin (αSMA) (ABCAM AB5964, Waltham, MA) and FN-EDA (Santa Cruz, IST-9, sc-59826, Dallas, Texas). Subsequently, ex vivo explants were incubated with rhodamine or Alexa Fluor 488-conjugated

(6) Methylglyoxal-Stimulated Mesothelial Cells Prompted Fibroblast-to-Proto-Myofibroblast TransitionInt J Mol SciJanuary 19, 2025Yu-Syuan Wei, Su-Yi Tsai, Shuei-Liong Lin, Yi-Ting Chen, Pei-Shiue Tsai(610077) was purchased from BD (Franklin Lakes, NJ, USA). Mouse monoclonal anti-EDA-fibronectin (EDAFN, sc-59826) was obtained from Santa Cruz Biotechnology (Dallas, TX, USA). Anti-phalloidin (BT00044) was purchased

(7) YAK577 Attenuates Cardiac Remodeling and Fibrosis in Isoproterenol-Infused Heart Failure Mice by Downregulating MMP12Korean Circulation JournalJanuary 1, 2025Hongyan Zhou, Hae Jin Kee, Le Wan, Yodita Asfaha, Fabian Fischer, Matthias U Kassack, Thomas Kurz, Seong Hoon Kim, Seung-Jung Kee, Young Joon Hong, et al.(sc-390863), anti-galectin3 (sc-32790), anti-HDAC8 (sc-374180), anti-Col1A1 (sc-293182), and anti-Fn (sc-59826) antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). The anti-natriuretic peptide

(8) SOCS domain targets ECM assembly in lung fibroblasts and experimental lung fibrosisScientific ReportsDecember 30, 2024Carina Magdaleno, Daniel J. Tschumperlin, Narendiran Rajasekaran, Archana Varadaraj#14179S), β Actin (Invitrogen, #PA5-59497), β Tubulin (Invitrogen, #MA5-16308), Fibronectin (Santa Cruz, #sc-59826), Collagen (Abcam, #ab34710), Smooth Muscle Actin (Abcam, #ab7817), Donkey Anti-Mouse Alexa Flour 568

(9) Cancer-associated fibroblasts derived fibronectin extra domain A promotes sorafenib resistance in hepatocellular carcinoma cells by activating SHMT1Genes & diseasesNovember 1, 2024Dong Y, Chen Y, Wang Y, Zhao X, Zi R, Hao J, Ding Q, Jiang H, Wang X, Lu F, et al.performed using a standard protocol. The antibodies included SHMT1 (Abcam, #ab186130), FN-EDA (Santa Cruz, #sc59826, RRID: AB_783389), and phosphorylated p65 (P-p65; CST, #3031S, RRID: AB_330559), fibronectin extra-domain

(10) Pirfenidone inhibits TGF-β1-induced fibrosis via downregulation of Smad and ERK pathway in MDCK cellsVeterinary Research CommunicationsOctober 1, 2024Chae-Yoon Im, Se-Hoon Kim, Ki-Hoon Song, Min-Ok Ryu, Hwa-Young Youn, Kyoung-Won Seoantibodies, such as anti-fibronectin (1:200 dilution, Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA, #sc-59826), anti-collagen3 (1:100 dilution, Santa Cruz Biotechnology, Inc., #sc-271249), anti-α-SMA (1:800 dilution,

(11) STING Promotes the Progression of ADPKD by Regulating Mitochondrial Function, Inflammation, Fibrosis, and ApoptosisBiomoleculesSeptember 26, 2024Jiao Wu, Shasha Cheng, Geoffray Lee, Ewud Agborbesong, Xiaoyan Li, Xia Zhou, Xiaogang Liincluded the following: (a) anti-STAT3 (sc-482), anti-p65 (sc372 and sc-8008), and anti-fibronectin (sc-59826) purchased from Santa Cruz Biotechnology Inc (Dallas, TX, USA); (b) anti-STING (13647), anti-cGAS (31659),

(12) The role of lipid peroxidation in epithelial–mesenchymal transition of retinal pigment epithelial cellsScientific ReportsJuly 17, 2024Wang You, Kunihiro Azuma, Toshiro Iwagawa, Sumiko Watanabe, Makoto Aihara, Tomoyasu Shiraya, Takashi Uetaunder a confocal microscope (LSM800, Zeiss). Primary antibodies used were as follows: Fibronectin (sc-59826. Santa Cruz), ZO-1 (61-7300, Invitrogen), and Vimentin (BS1491, Bioworld). Secondary antibodies: Alexa

(13) circ-hnRNPU inhibits NONO-mediated c-Myc transactivation and mRNA stabilization essential for glycosylation and cancer progressionJournal of Experimental & Clinical Cancer ResearchNovember 23, 2023Hongjun Li, Wanju Jiao, Jiyu Song, Jianqun Wang, Guo Chen, Dan Li, Xiaojing Wang, Banghe Bao, Xinyi Du, Yang Cheng, et al.(ab36415), polypeptide N-acetylgalactosaminyl-transferase 6 (GALNT6, ab151329), fibronectin 1 (FN1, sc-59826, Santa Cruz Biotechnology, Santa Cruz, CA), alpha-1,3-mannosyl-glycoprotein 2-beta-N- acetylglucosaminyltransferase

(14) Protocatechuic acid prevents isoproterenol-induced heart failure in mice by downregulating kynurenine-3-monooxygenaseJournal of cellular and molecular medicineAugust 1, 2023Bai L, Han X, Kee HJ, He X, Kim SH, Jeon MJ, Zhou H, Jeong SM, Kee SJ, Jeong MH.antibody were obtained from Sigma‐Aldrich. Anti‐Actb (cat no. sc‐47778, lot no. K2222), anti‐Fn1 (cat no. sc‐59826, lot no. F1814), anti‐Col1a1 (cat no. sc‐293182, lot no. H2619) and anti‐BNP (Nppb, sc‐271185, lot no.

(15) Changes in nascent chromatin structure regulate activation of the pro-fibrotic transcriptome and myofibroblast emergence in organ fibrosisiScienceMay 19, 2023Basta MD, Petruk S, Summer R, Rosenbloom J, Wermuth PJ, Macarak E, Levin AV, Mazo A, Walker JL.

(16) ALK5 Inhibition of Subconjunctival Scarring From Glaucoma Surgery: Effects of SB-431542 Compared to Mitomycin C in Human Tenon's Capsule FibroblastsTranslational Vision Science & TechnologyFebruary 24, 2023Jack E. Teplitsky, Anastasiya Vinokurtseva, James J. Armstrong, James Denstedt, Hong Liu, Cindy M. L. Hutnikhour. Primary antibodies used for MMP-9, α-SMA, and EDA-FN (ab38898, ab5694, abcam, Cambridge, UK; sc-59826, Santa Cruz Biotechnology, Dallas, TX, USA) were prepared as 1/1000, 1/500, and 1/200 dilutions, respectively.

(17) Molecular mechanisms regulating wound repair: Evidence for paracrine signaling from corneal epithelial cells to fibroblasts and immune cells following transient epithelial cell treatment with Mitomycin CExperimental eye researchFebruary 1, 2023Pal-Ghosh S, Karpinski BA, Datta Majumdar H, Ghosh T, Thomasian J, Brooks SR, Sawaya AP, Morasso MI, Scholand KK, de Paiva CS, et al.

(18) The Fibrotic Effects of LINC00663 in Human Hepatic Stellate LX-2 Cells and in Bile Duct-Ligated Cholestasis Mice Are Mediated through the Splicing Factor 2-FibronectinCellsJanuary 4, 2023Chu Y, Bao L, Teng Y, Yuan B, Ma L, Liu Y, Kang H.Cambridge, UK, ab38017), FN (1:5000, Abcam, ab45688), extra domain A-FN (EDA-FN; 1:500, Santa Cruz, sc-59826) and FOXA1 (1:5000, Abcam, ab170933). 2.9. Cell Transfection Small interfering (si)-LINC00663, pcDNA-LINC00663,

(19) Targeting the extra domain A of fibronectin for cancer therapy with CAR-T cellsJournal for ImmunoTherapy of CancerAugust 2, 2022Celia Martín-Otal et al.added and incubated at 5 µg/mL overnight at 4°C. When indicated, the anti-EDA antibody used was IST-9 (sc-59826, Santa Cruz) at the same concentration. After washing with TBS solution, slides were incubated with

(20) Syringic acid mitigates isoproterenol-induced cardiac hypertrophy and fibrosis by downregulating EregJournal of cellular and molecular medicineJuly 1, 2022Han X, Bai L, Kee HJ, Jeong MH.

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Fibronectin (IST-9) Antibody

Fibronectin (IST-9) Antibody from Santa Cruz Biotechnology, Inc.

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Santa Cruz Biotechnology, Inc.

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Fibronectin (IST-9) Antibody from Santa Cruz Biotechnology, Inc.

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