Good Fibronectin Antibody for Western Blot

Nanomedicine
Lazar Research Laboratories
Clinical Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-Fibronectin antibody

Catalog Number:

ab2413

Clone Number:

N/A

I tried this antibody several times on fibroblast and epithelial cells and I got good data. Our research is focused on the SMAD pathway that regulates a diverse set of biological processes, including proliferation, differentiation and apoptosis, in many different organ systems. TGF-ß ligands that activate the SMAD-2/3 intracellular pathway have been heavily implicated in fibrosis.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Fibroblast

Primary Incubation

1:1000, 4 °C, overnightù

Blocking Agent

BSA 5% TBST

Secondary Incubation

1:10000

Tertiary Incubation

N/A

Detection

Chemiluminescence

Results Summary

Data are good. I overexpressed fibronectin with TGF-b and band are clear.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Band is clear.

The Bad

N/A

The Bottom Line

Really recommended

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