Anti-GFAP antibodies are essential reagents in flow cytometry for detecting the target protein, glial fibrillary acidic protein. GFAP is an intermediate filament protein expressed in a variety of cell types, including astrocytes, ependymal cells, peritubular fibroblasts, Leydig cells of the testis, keratinocytes, osteocytes and chondrocytes. It can be used to distinguish astrocytes from other glial cells. GFAP localizes to the cytoplasm. Examine the supplier's provided validation data when comparing GFAP antibodies for flow cytometry. Also look for application-specific recommendations, such as dilutions, protocols, sample types, and other helpful usage notes.