GFAP Polyclonal Antibody bs-0199R from Bioss Inc.

Product Specs
ItemGFAP Polyclonal Antibody
CompanyBioss Inc.
Price $375.00Supplier Page View Company Product Page
Catalog Numberbs-0199R
Quantity100ul, 20uL
ApplicationsIF(IHC-P), IF(IHC-F), WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), WB, IHC-P, IF(IHC-P), IF(ICC), Others, IHC, IHC-P, WB, IHC-P
Conjugate/TagUnconjugated
Format0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol
Concentration1ug/ul
Molecular Weight48kDa
IsotypeIgG
ReactivityHuman, Mouse, Rat, Human, Mouse, Rat, Human, Other Species, Mouse
Antibody TypePrimary Antibody
TargetGFAP
HostRabbit
Tissuebone marrow cells, Brain, braiN/Astrocytes, brain, brain (cortex), Other
ImmunogenKLH conjugated peptide, human GFAP
ClonalityPolyclonal
NCBI Full Gene Nameglial fibrillary acidic protein
NCBI Gene AliasesALXDRD

Questions and Answers

Can this antibody be used for immunohistochemistry in brain tissue?

Yes, the GFAP Polyclonal Antibody has been utilized in immunohistochemistry applications, as demonstrated in studies examining brain tissue, including those investigating tau accumulations in woodpeckers and retinal ganglion cell apoptosis in glaucomatous rats (, ).

What species does this antibody react with, and how has it been applied in research?

The GFAP Polyclonal Antibody is reactive with mouse, human, and rat tissues. It has been shown to work in various applications, including studies on neural stem cells and astrocyte activation in models of neurological diseases (, ).

Is this antibody effective for flow cytometry applications?

Yes, the GFAP Polyclonal Antibody has been cited in studies that employed flow cytometry to assess glial fibrillary acidic protein expression in neural stem cells and other cell types ().

What is the significance of using this antibody in studies related to neuroinflammation?

The GFAP Polyclonal Antibody is crucial for identifying astrocyte activation, which is a key marker of neuroinflammation. Its application in various studies has helped elucidate mechanisms of neuroinflammatory responses in conditions such as spinal cord injury and Alzheimer's disease (, ).

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Woburn, MA 01801

United States

Phone: 800.501.7654 (Toll free)
Phone: +1.781.569.5821 (International)

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Company Profile

Website: www.biossusa.com

(1) TTNPB Promotes Human Pluripotent Stem Cell‐to‐Neural Stem Cell Transition via Modulation of Chromatin Accessibility and the S‐(5′‐adenosyl)‐L‐homocysteine/Choline Metabolic NetworkAdvanced ScienceFebruary 10, 2026Ruilin Du, Yudi Ren, Qiaoqiao Meng, Peng Wei, Ruyu Zhu, Junjie Bao, Ye Yang, Shuo Yan, Chaorong Yue, Xueying Zhu, et al.#93972, 1:100), mouse monoclonal TUBB3 (Bioss, #BSM‐33177M, 1:200), rabbit polyclonal GFAP (Bioss, #bs‐0199R, 1:200), and rabbit monoclonal CDX2 (Biogenex, #Cdx2‐88). The secondary antibodies used were Alexa Fluor

(2) Long‐Term Stable Subdural Recordings Enabled by Fibrosis‐Resistant Hydrogel‐Integrated µECoG ArraysAdvanced ScienceOctober 8, 2025Lin Chen, Hao Zhong, Linghao Wang, Liju Xu, Wanying Fan, Yongpeng Zhao, Huiling Zhang, Yang Shen, Kai Wu, Xin Fu, et al.incubated overnight at 4 °C with the following primary antibodies: rabbit anti‐GFAP (1:500, Bioss #bs‐0199R‐1, USA), goat anti‐Iba1 (1:500, Abcam #ab289874, USA), and rat anti‐NeuN (1:500, Abcam #ab279297, USA).

(3) Soluble N-terminal region of prion protein causes rapid neurodegeneration in prion diseaseSci AdvAugust 8, 2025Runchuan Yan, Yan Zhang, Jingjing Zhang, Xiangyi Zhang, Yue Han, Mengfei Wang, Dan Wang, Shengnan Huang, Wei Liu, Qi Shi, et al.no. ab289874; 1:1500) and anti–glial fibrillary acidic protein (GFAP) antibody (Bioss, catalog no. bs-0199R; 1:500). The sections were then washed in PBS with 0.2% Tween and incubated with a secondary antibody

(4) Endoplasmic reticulum stress of astrocytes in paraventricular nucleus of hypothalamus promotes ventricular electrical instability after acute myocardial infarction in ratsFront Cardiovasc MedJuly 7, 2025Yuhang Yang, Xinmiao Huang, Wenlong Wang, Jingmei Sun, Xin Wang, Chunrui Ji, Xiufen Qu, Dechun Yinfrom gels, which were included with primary antibody including Rabbit anti GFAP polyclonal antibody (bs-0199r; BIOSs, China, 1:400), Rabbit anti Fos monoclonal antibody (1:1000), rabbit anti-C3 (1:1000), Rabbit

(5) Astrocyte-Derived Extracellular Vesicles Alleviate Optic Nerve Injury Through Remodeling of Retinal Microenvironmental HomeostasisInvest Ophthalmol Vis SciApril 7, 2025Lili Chen, Zhonghao Yu, Senmiao Zhu, Shihan Song, Guanwen He, Zai-Long Chi, Wencan WuChina" Calnexin,Rabbit monoclonal,10427-2-AP,1:1000,"Proteintech, Chicago, IL, USA" GFAP,Rabbit monoclonal,bs-0199R,1:500,"Bioss, Beijing, China" Vimentin,Mouse monoclonal,sc-6260,1:500,"Santa Cruz, Dallas, Texas, USA" RBPMS,Rabbit

(6) Investigating the In Vivo Effects of Anti-Prion Protein Nanobodies on Prion Disease with AAV VectorPathogensFebruary 2, 2025Jingjing Zhang, Mengfei Wang, Dan Wang, Xiangyi Zhang, Yue Ma, Els Pardon, Jan Steyaert, Romany Abskharon, Fei Wang, Jiyan Maincluded rabbit anti-Iba1 (1:1500, cat#ab178846, Abcam, Cambridge, UK), rabbit anti-GFAP (1:500, cat#bs-0199R, Bioss, Beijing, China), and SAF84 mouse anti-PrP (1:500, cat#189775, CAYMAN, Ann Arbor, MI, USA). 2.6.

(7) Small extracellular vesicles derived from human induced pluripotent stem cell-differentiated neural progenitor cells mitigate retinal ganglion cell degeneration in a mouse model of optic nerve injuryNeural regeneration researchFebruary 1, 2025Li T, Xing HM, Qian HD, Gao Q, Xu SL, Ma H, Chi ZL.USA",15187-1-AP,AB_2238431,IF GFAP,Glial fibrillary acidic protein,Rabbit,1:1000,"Bioss Biotechnology, Beijing, China",bs-0199R-1,,IF Goat anti-rabbit IgG H&L/Alexa Fluor 488,,Goat Antianti-Rabbit rabbit,1:500,Bioss Biotechnology,bs-0295G-A

(8) FoxG1 as a Potential Therapeutic Target for Alzheimer’s Disease: Modulating NLRP3 Inflammasome via AMPK/mTOR Autophagy PathwayCellular and Molecular NeurobiologyDecember 1, 2024Qi Yun, Si-Fei Ma, Wei-Ning Zhang, Meng Gu, Jia Wang(WL01637), and rabbit anti-Bcl2 (WL01556) antibodies from Wanleibio (ShengYang, China); rabbit anti-GFAP (bs-0199R) antibodies from Bioss Biotechnology Co. (Beijing, China); rabbit anti-Beclin1(A7353) and rabbit anti-NLRP3(A5652)

(9) Qingzhuan dark tea Theabrownin alleviates hippocampal injury in HFD-induced obese mice through the MARK4/NLRP3 pathwayHeliyonMarch 15, 2024Yining Lei, Yong Chen, Shuo Zhang, Wei Wang, Min Zheng, Ruyi Zhanganti-NLRP3 (PAB38738, Bioswamp, 1:200), rabbit anti-MARK4 (AF0693, Affinity, 1:200), rabbit anti-GFAP (bs-0199R, Bioss, 1:200), rabbit anti-Iba1 (A19776, ABconal, 1:200), rabbit anti-BAX (bs-0127R, Bioss, 1:200).

(10) Deubiquitinase Mysm1 regulates neural stem cell proliferation and differentiation by controlling Id4 expressionCell death & diseaseFebruary 12, 2024Xu Z, Qin Q, Wang Y, Zhang H, Liu S, Li X, Chen Y, Wang Y, Ruan H, He W, et al.doublecortin (Dcx, #91954, Cell Signaling Technology), Aldh1l1 (#85828, Cell Signaling Technology), Gfap (bs-0199R, Bioss), Olig2 (#65915, Cell Signaling Technology), NG2 (ab255811, Abcam), Id4 (sc-365656, Santa Cruz Biotechnology

(11) Argatroban promotes recovery of spinal cord injury by inhibiting the PAR1/JAK2/STAT3 signaling pathwayNeural regeneration researchFebruary 1, 2024Zhao C, Zhou T, Li M, Liu J, Zhao X, Pang Y, Liu X, Zhang J, Ma L, Li W, et al.(western blotting) Anti-Vimentin,Rabbit,Abcam,ab92547,AB_10562134,1:200 (Immunofluorescence staining) Anti-GFAP,Rabbit,"Bioss, Beijing, China",bs-0199R,NA,1:200 (Immunofluorescence staining) Anti-PAR1,Mouse,Santa Cruz Biotechnology,sc-13503,NA,1:200 (Immunofluorescence

(12) Neurovascular Unit Compensation from Adjacent Level May Contribute to Spontaneous Functional Recovery in Experimental Cervical Spondylotic MyelopathyInternational Journal of Molecular SciencesFebruary 8, 2023Guang-Sheng Li, Guang-Hua Chen, Kang-Heng Wang, Xu-Xiang Wang, Xiao-Song Hu, Bo Wei, Yong Hubs-11194R, 1:200), Anti-AIF1/Iba1 antibody (Bioss, bs-1363R, 1:200) and Anti-GFAP antibody (Bioss, bs-0199R, 1:400) at 4 °C overnight. The primary antibody substituted for PBS was set for the negative control.

(13) Genetic and Pharmacological Inhibition of Astrocytic Mysm1 Alleviates Depressive-Like Disorders by Promoting ATP ProductionAdvanced science (Weinheim, Baden-Wurttemberg, Germany)November 22, 2022Zhang H, Liu S, Qin Q, Xu Z, Qu Y, Wang Y, Wang J, Du Z, Yuan S, Hong S, et al.anti‐rabbit IgG, 406421, Biolegend). After washing, antibodies against astrocytes (Rabbit Anti‐ GFAP/AF488, bs‐0199R‐AF488, Bioss) and microglia cells (APC anti‐mouse CX3CR1, 149007, Biolegend) were added and incubated.

(14) <italic>MCU</italic> knockdown in hippocampal neurons improves memory performance of an Alzheimer’s disease mouse modelActa Biochimica et Biophysica SinicaOctober 25, 2022Hongyan Cai, Jing Qiao, Siru Chen, Junting Yang, Christian Hölscher, Zhaojun Wang, Jinshun Qi, Meina WuBeijing, China) for 30 min. Afterwards, sections were incubated with the anti-GFAP primary antibody (bs-0199R, 1:200; Bioss, Beijing, China) at 4°C overnight, followed by treatment with the peroxidase-conjugated

(15) Astrocytic SARM1 promotes neuroinflammation and axonal demyelination in experimental autoimmune encephalomyelitis through inhibiting GDNF signalingCell death & diseaseSeptember 2, 2022Jin L, Zhang J, Hua X, Xu X, Li J, Wang J, Wang M, Liu H, Qiu H, Chen M, et al.Sigma-Aldrich) in 5% BSA for 1.5 h at room temperature. The primary antibodies include rabbit anti-GFAP (1;500, bs-0199R, Bioss), mouse anti-GDNF (1:500, sc-13147, SANTA CRUZ). The secondary antibodies included donkey antimouse

(16) Targeting microglial autophagic degradation of the NLRP3 inflammasome for identification of thonningianin A in Alzheimer's diseaseInflammation and regenerationAugust 3, 2022Zhou XG, Qiu WQ, Yu L, Pan R, Teng JF, Sang ZP, Law BY, Zhao Y, Zhang L, Yan L, et al.(Cambridge, MA, USA). Anti-TREM2 (29208) was purchased from Signalway Antibody (Maryland, USA). Anti-GFAP (bs-0199R) was obtained from Bioss (Beijing, China). All antibodies were commercially sourced and their specificity

(17) Delayed administration of nafamostat mesylate inhibits thrombin-mediated blood-spinal cord barrier breakdown during acute spinal cord injury in ratsJournal of neuroinflammationJuly 16, 2022Zhao C, Zhou T, Zhao X, Pang Y, Li W, Fan B, Li M, Liu X, Ma L, Zhang J, et al.were sliced into 8 μm. Slices were incubated with the following rat-specific primary antibodies: GFAP (bs-0199R, Bioss, China), NeuN (ab177487, Abcam, USA), Anti-CD31 antibody (ab24590, Abcam, USA), Anti-CD31 (bs-0195R,

(18) Correlation Analysis Between Magnetic Resonance Imaging-Based Anatomical Assessment and Behavioral Outcome in a Rat Contusion Model of Chronic Thoracic Spinal Cord InjuryFrontiers in NeuroscienceApril 21, 2022Cong Xing, Zeyu Jia, Haodong Qu, Song Liu, Wang Jiang, Hao Zhong, Mi Zhou, Shibo Zhu, Guangzhi Ning, Shiqing Fengsamples were manufactured to be probed with anti-glial fibrillary acidic protein (GFAP) antibody (1:500, Bs-0199r, Bioss, Beijing, China) and goat anti-rabbit IgG Alexa 555 (1:500, ab150086, Abcam, Cambridge, United

(19) LncRNA SNHG3 promotes gastric cancer cell proliferation and metastasis by regulating the miR-139-5p/MYB axisAging (Albany NY)December 15, 2021Yan Xie, Li Rong, Min He, Yuyou Jiang, Haiyu Li, Li Mai, Fangzhou Songblots were incubated overnight with anti-MYB (1:1000, ab45150, Abcam, MA, USA) or anti-GAPDH (1:1000, bs-0199R, Bioss, Beijing, China) at 4° C. Following incubation with an appropriate secondary antibody, a chemiluminescence

(20) TRPV4-induced Müller cell gliosis and TNF-α elevation-mediated retinal ganglion cell apoptosis in glaucomatous rats via JAK2/STAT3/NF-κB pathwayJournal of neuroinflammationNovember 17, 2021Li Q, Cheng Y, Zhang S, Sun X, Wu J.Alomone Labs), anti-glutamine synthetase (GS, cat. no. GTX109121, 1:400, GeneTex), anti-GFAP (cat. no. bs-0199R, 1:500, Bioss), and anti-NF-κB p65 (cat. no.sc-372, 1:200, Santa Cruz). As negative controls, TRPV4

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(1)

5/23/2023Good GFAP Antibody for IHC from Bioss Mehmet Fatih BozkurtI had good results using the standard citrate buffer pH 6.0 antigen method on formol-fixed paraffin-blocked tissues. I don't think any other application is needed.

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GFAP Polyclonal Antibody

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IF(ICC)

GFAP Polyclonal Antibody from Bioss Inc.

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GFAP Polyclonal Antibody from Bioss Inc.

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