Fig 1: SR-induced BBB damage and astrocyte death was ameliorated by SI. Images of representative tissue slices within the limbic area of the brain showing quadruple-label immunofluorescence stained with GFAP (green), fibrinogen (yellow), Glut-1 (red), and DAPI (blue) displaying differences in vascular permeability in each treatment group. All images are at 40× magnification. Scale bar = 80 µm.
Fig 2: SI and SR differentially altered astrocyte morphology. Immunofluorescent images of individual astrocytes stained with GFAP (green) displaying differences in astrocyte morphology in each treatment group. Additional background staining includes fibrinogen (yellow), Glut-1 (red), and DAPI (blue). All images were acquired at 40× magnification and zoomed to focus on a single astrocyte. Scale bar = 5 µm.
Supplier Page from Novus Biologicals, a Bio-Techne Brand for GFAP Antibody (SPM248) [Alexa Fluor® 488]
Available conjugates: Available conjugates: FITC, DyLight 650, Alexa Fluor 700, DyLight 405, Alexa Fluor 405, Alexa Fluor 532, DyLight 755, Alexa Fluor 488Specificity: This monoclonal antibody recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.Sizes Available: 0.1 ml