Fig 1: Multiparametric analysis reveals elevated protein synthesis coupled to altered proliferation and neuronal cell fate decisions in FXS patient-derived cells(A) Schematic outlining the neuronal optimized multicolor immunophenotyping panel (neurOMIP) assay.(B) Increased translation within NESTIN+/Ki67+ actively proliferating cells and higher abundance of proliferative cells in FXS cultures compared with that of controls. NESTIN+/SOX2+ cells (top left) and NESTIN+/DCX+ cells (bottom left) showed significantly increased protein synthesis in FXS cultures compared with that of controls. NESTIN+/SOX2+ (top right) and NESTIN+/DCX+ (bottom right) cells both showed increased abundance in FXS cultures.(C) No significant difference in abundance or protein synthesis in NESTIN-/Ki67- cells between control and FXS cultures. Ki67-/DCX+/MAP2+ cells (top left) and Ki67-/GFAP-/MAP2+ cells (bottom left) showed similar levels of protein synthesis across control and FXS cultures. Ki67-/DCX+/MAP2+ cells (top right) and Ki67-/GFAP-/MAP2+ cells (bottom right) were reduced in FXS cultures. Each subpopulation was analyzed using Mann-Whitney tests, n = 4 CTR, 6 FXS, *p < 0.05, **p < 0.01). Data are shown as means ± SEM.(D) Summary of abundance of proliferative and non-proliferative populations in CTR versus FXS patient-derived cultures.
Supplier Page from Novus Biologicals, a Bio-Techne Brand for GFAP Antibody (SPM248) [Alexa Fluor® 700]
Available conjugates: Available conjugates: FITC, DyLight 405, Alexa Fluor 405, DyLight 755, Alexa Fluor 700Specificity: This monoclonal antibody recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.Sizes Available: 0.1 ml