GFAP Antibody NB100-53809 from Novus Biologicals, a Bio-Techne Brand

Product Specs
ItemGFAP Antibody
CompanyNovus Biologicals, a Bio-Techne Brand
Price $429.00Supplier Page View Company Product Page
Catalog NumberNB100-53809
ImmunogenThis GFAP Antibody was developed against a peptide with sequence C-DGEVIKESKQEHKD corresponding to C-Terminus according to NP_002046.1
Concentration0.5 mg/ml
FormatTris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Conjugate/TagUnconjugated
TargetGFAP
Quantity0.1 mg
HostGoat
ApplicationsIF/IHC,Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Peptide ELISA,Immunohistochemistry Free-Floating,Immunohistochemistry Whole-Mount
ReactivityHuman,Mouse,Rat
NCBI Full Gene Nameglial fibrillary acidic protein
ClonalityPolyclonal
NCBI Gene AliasesALXDRD
PurityImmunogen affinity purified
Research AreaAstrocyte Markers, Cancer, Cell Biology, Cellular Markers, Chaperone Mediated Autophagy (CMA), Inflammation, Neurodegeneration, Neuroscience, Signal Transduction, Stem Cell Markers
IsotypeIgG
Antibody TypePrimary Antibodies
Molecular Weight50 kDa
StorageStore at -20C. Avoid freeze-thaw cycles

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: GFAP is thought to help to maintain astrocyte mechanical strength, as well as the shape of cells but its exact function remains poorly understood, despite the number of studies using it as a cell marker.

Sizes Available: 0.1 mg

Add to Compare List

Questions and Answers

Can this GFAP antibody be used for immunohistochemistry in mouse models?

Yes, the GFAP antibody has been successfully used for immunohistochemistry in mouse models, as demonstrated in studies investigating brain responses to various treatments, including gene delivery methods and neurodevelopmental impacts of chemical exposure (, ).

What specific applications has this antibody been shown to work in?

The GFAP antibody has been shown to work in several applications, including Western blotting, ELISA, flow cytometry, immunofluorescence, and immunohistochemistry, making it versatile for various experimental setups in neuroscience research.

Is this antibody effective for detecting GFAP in both human and rodent tissues?

Yes, the GFAP antibody is reactive to glial fibrillary acidic protein in both human and rodent tissues, as indicated by its use in studies involving mouse models and human-related research (, ).

How has this antibody contributed to research on astrocyte function and brain injury?

The GFAP antibody has been utilized to assess astrocyte activation and response in various contexts, such as after subarachnoid hemorrhage and exposure to neurotoxic substances, highlighting its role in understanding astrocyte function and brain injury mechanisms (, ).

Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: https://www.novusbio.com

(1) Pericytes are organ-specific regulators of tissue morphogenesisNat CommunMay 12, 2026Seyed Javad Rasouli, Kai Kruse, Rodrigo Diéguez-Hurtado, Parisa Ghanbari, Anusha Aravamudhan, Mara E. Pitulescu, Ralf H. AdamsAbcam, ab110639), rabbit anti-GFAP (1:200, DAKO, Z0334), goat anti-GFAP (1:200, Novus Biologicals, NB100-53809), rat anti-CD68 (1:100, Abcam, ab53444), chicken anti-GFP (1:200, 2BScientific Ltd., GFP-1010), rabbit

(2) Stroke Exacerbates Respiratory Disorder and Cognition Impairment in Mice with Cerebral Amyloid Angiopathyaging and diseaseJune 23, 2025YuXing Zhang, Ahmad El Hamamy, Zahid Iqbal, Arya Ranjan, Destiny Sumani, Hung Wen Lin, Louise D. McCullough, Jun LiAβ (1:300, Abcam, Cat# ab201060), Phox2B (1:20, Bio-Techne, Cat# AF4940), GFAP (1:200, Novus, Cat# NB100-53809), C3 (1:200, Abcam, Cat# ab97462) S100A10 (1:200, Invitrogen, Cat# PA5-95505), LYVE1(1:200, CST, Cat#

(3) Stroke Exacerbates Respiratory Disorder and Cognition Impairment in Mice with Cerebral Amyloid AngiopathyAging DisMay 25, 2025YuXing Zhang, Ahmad El Hamamy, Zahid Iqbal, Arya Ranjan, Destiny Sumani, Hung Wen Lin, Louise D. McCullough, Jun LiAβ (1:300, Abcam, Cat# ab201060), Phox2B (1:20, Bio-Techne, Cat# AF4940), GFAP (1:200, Novus, Cat# NB100-53809), C3 (1:200, Abcam, Cat# ab97462) S100A10 (1:200, Invitrogen, Cat# PA5-95505), LYVE1(1:200, CST, Cat#

(4) Single-cell sequencing reveals intracranial microvasculature-derived CXCL12 promotes CD8+ T-cell infiltration and blood-brain barrier dysfunction after subarachnoid hemorrhage in miceJ NeuroinflammationApril 23, 2025Yuanshu Li, Xufang Ru, Ya Xu, Peiwen Guo, Jiru Zhou, Weina Li, Mingxu Duan, Wenbo Kang, Jie Lin, Xuyang Zhang, et al.MA1-33449, Invitrogen), mouse anti-Iba-1 (1:500, GB12105, ServiceBio, Wuhan, China), goat anti-GFAP (1:400, NB100-53809, Novus Biologicals, Shanghai, China), rabbit anti-CXCL12 (1:200, YT4225, Immunoway), rabbit anti-CD8

(5) Cross-disorder and disease-specific pathways in dementia revealed by single-cell genomicsCellOctober 3, 2024Jessica E. Rexach, Yuyan Cheng, Lawrence Chen, Damon Polioudakis, Li-Chun Lin, Vivianne Mitri, Andrew Elkins, Xia Han, Mai Yamakawa, Anna Yin, et al.,Atlas antibodies,HPA018039;RRID:AB_1852125 Guineapig NeuN,Synaptic Systems,266004;RRID:AB_2619988 Goat GFAP,Novus biologicals,NB100–53809;RRID:AB_829022 Rabbit Anti-Optineurin Antibody (C-2),Fisher,PA5–28249;RRID:AB_2545725 Mouse Anti-Protein

(6) Delivery of CDNF by AAV-mediated gene transfer protects dopamine neurons and regulates ER stress and inflammation in an acute MPTP mouse model of Parkinson's diseaseScientific reportsJuly 17, 2024Nam J, Richie CT, Harvey BK, Voutilainen MH.ab21685, RRID:AB_2119834), goat anti-glial fibrillary acidic protein (GFAP, 1:500, Novus Biological, NB100-53809, RRID:AB_829022), goat anti-Ionized calcium-binding adaptor molecule 1 (Iba1, 1:500, Wako, 011-27991),

(7) Male mice are susceptible to brain dysfunction induced by early-life acephate exposureFrontiers in NeuroscienceJuly 10, 2024Takahiro Sasaki, Jahidul Islam, Kenshiro Hara, Tomonori Nochi, Kentaro Tanemuradiluted 1:200), and goat polyclonal anti-glial fibrillary acidic protein (GFAP; Novus Biologicals; NB100-53809; diluted 1:200). After rinsing with phosphate-buffered saline, immunoreactive elements were visualized

(8) Sarcosine Suppresses Epileptogenesis in Rats With Effects on Hippocampal DNA MethylationFrontiers in molecular neuroscienceJanuary 1, 0001"Shen HY, Weltha L, Cook JM, Gesese R, Omi W, Baer SB, Rose RM, Reemmer J, Boison D.""Active Motif, Carlsbad, CA",1:3000 (IHC) NeuN,MAB-377,"Millipore Sigma, Burlington, MA",1:500 (IHC) GFAP,NB100-53809,"Novus Biologicals, Centennial, CO",1:1000 (IHC) TET1,PA5-72805,"Thermo Fisher Scientific, Waltham,

(9) Assessing the Nature of Human Brain-Derived Extracellular Vesicles on Synaptic Activity Via the Development of an Air-liquid Microfluidic Platform"Advanced science (Weinheim, Baden-Wurttemberg, Germany)"January 1, 0001"Bernou C, Iwasaki Y, Lutz W, Almeida G, Béchard E, Delalande F, Rompais M, Bouget J, Gorda B, Cazevieille C, et al."Neurofilament H (NF‐H, 1:200, Synaptic Systems #171121), goat anti GFAP (1:400, Novus Biologicals #nb100‐53809), and rabbit anti Iba1 (1:400, Genetex, GTX101495). After three washes with blocking solution and secondary

(10) Deficiency of astrocyte CysLT(1)R ameliorates depression-like behaviors in mice by modulating glutamate synaptic transmissionNeurobiology of diseaseJanuary 1, 0001"Liu X, Tang SS, Liu SM, Zeng J, Chen ZG, Liu CH, Mu RH, Yuan DH, Zhao JJ, Hong H, et al."

(11) Lipid Deposition Profiles Influence Foreign Body Responses"Advanced materials (Deerfield Beach, Fla.)"January 1, 0001"Schreib CC, Jarvis MI, Terlier T, Goell J, Mukherjee S, Doerfert MD, Wilson TA, Beauregard M, Martins KN, Lee J, et al."1xPBST overnight in the 4°C fridge. The primary antibodies used were GFAP (1:250; Novus Biologicals NB100–53809) and CD68 (1:250; Novus Biologicals NBP2–33337). The following day, the mounted slides were washed with

(12) Organotypic culture of human brain explants as a preclinical model for AI-driven antiviral studiesEMBO molecular medicineJanuary 1, 0001"Partiot E, Gorda B, Lutz W, Lebrun S, Khalfi P, Mora S, Charlot B, Majzoub K, Desagher S, Ganesh G, et al."#GTX133109), rat anti-CTIP2 (1/100, BioLegend, clone [25B6]), goat anti-GFAP (1/100, Novus Biologicals, #NB100-53809), mouse anti-Tuj1 (1/100, Genetex, #GTX631836), rabbit anti-Iba1 (1/100, Genetex, #GTX101495), mouse

(13) Deletion or Inhibition of Astrocytic Transglutaminase 2 Promotes Functional Recovery after Spinal Cord InjuryCellsJanuary 1, 0001"Elahi A, Emerson J, Rudlong J, Keillor JW, Salois G, Visca A, Girardi P, Johnson GVW, Pröschel C."stained with: rabbit polyclonal (pAb) GFAP (1:1000, Dako, Z033401-2), goat pAb GFAP (1:300, Novus, NB100-53809), rabbit pAb SOX9 (1:50, Millipore Sigma, Burlington, MA, USA, AB5535), chicken pAb NeuN (1:2000, Millipore Sigma

(14) Evolutionarily conserved regulators of tau identify targets for new therapiesNeuronJanuary 1, 0001"Kim J, de Haro M, Al-Ramahi I, Garaicoechea LL, Jeong HH, Sonn JY, Tadros B, Liu Z, Botas J, Zoghbi HY."

(15) Transgene distribution and immune response after ultrasound delivery of rAAV9 and PHP.B to the brain in a mouse model of amyloidosisMolecular therapy. Methods & clinical developmentJanuary 1, 0001"Kofoed RH, Heinen S, Silburt J, Dubey S, Dibia CL, Maes M, Simpson EM, Hynynen K, Aubert I."(Millipore; ABN90), rabbit anti-IBA1 1:500 (Wako; 016-20001), goat anti-GFAP 1:1,000 (Novus Biologicals; NB100-53809), rabbit anti-S100B 1:800 (Abcam; ab41548), goat anti-complement component C3d 1:200 (R&D Systems; AF2655),

(16) Noninvasive delivery of an α-synuclein gene silencing vector with magnetic resonance-guided focused ultrasoundMovement disorders : official journal of the Movement Disorder SocietyJanuary 1, 0001"Xhima K, Nabbouh F, Hynynen K, Aubert I, Tandon A."RT. Sections were incubated with anti-Iba-1 antibody (019–19741, 1:1000; Wako Chemicals) and anti-GFAP (NB100–53809, 1:1000; Novus Biologicals) for 24 h at 4°C followed by secondary antibodies for 2 h and DAPI for 10

(17) Expression of the human herpesvirus 6A latency-associated transcript U94A impairs cytoskeletal functions in human neural cellsMolecular and cellular neurosciencesJanuary 1, 0001"Hogestyn JM, Salois G, Xie L, Apa C, Youngyunpipatkul J, Pröschel C, Mayer-Pröschel M."

(18) The engineered AAV2-HBKO promotes non-invasive gene delivery to large brain regions beyond ultrasound targeted sitesMolecular therapy. Methods & clinical developmentJanuary 1, 0001"Kofoed RH, Noseworthy K, Wu K, Sivadas S, Stanek L, Elmer B, Hynynen K, Shihabuddin LS, Aubert I."ab13970), guinea pig anti-NeuN 1:500 (Millipore, ABN90), goat anti-GFAP 1:1,000 (Novus Biologicals, NB100-53809), rabbit anti-human fibrinogen 1:400 (Dako, A0080), rat anti-Ter119 1:200 (BD Biosciences, 553671),

(19) Endothelial EGLN3-PKM2 signaling induces the formation of acute astrocytic barrier to alleviate immune cell infiltration after subarachnoid hemorrhageFluids and barriers of the CNSJanuary 1, 0001"Duan M, Ru X, Zhou J, Li Y, Guo P, Kang W, Li W, Chen Z, Feng H, Chen Y."claudin 1 (1:500, 37-4900, Invitrogen), connexin 43 (1:500, C6219-100, Sigma‒Aldrich), GFAP (1:800, NB100-53809, Novus), CD 31 (1:100, NB100-2284, NOVUS), ZO 1 (1:100, NBP 1-85046, NOVUS), CD45 (1:500, 70257S, Cell Signaling Technology

(20) MORPHIOUS: an unsupervised machine learning workflow to detect the activation of microglia and astrocytesJournal of neuroinflammationJanuary 1, 0001"Silburt J, Aubert I."1:1500), goat anti-GFAP (Santacruz Biotech, cat: sc-6170; 1:250), goat anti-GFAP (Novus Biological, cat: NB100-53809; 1:2000), rabbit anti-TGFβ1 (Abcam, cat: ab215715; 1:250), rat anti-CD68 (Biolegend, cat: 137002, 1:400),

Be the First to Write a Review!

GFAP Antibody

GFAP Antibody

GFAP Antibody

GFAP Antibody

GFAP Antibody from Novus Biologicals, a Bio-Techne Brand

Specifications/Features

Questions and Answers

Novus Biologicals, a Bio-Techne Brand

Citations (28)

Reviews

GFAP Antibody from Novus Biologicals, a Bio-Techne Brand

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map