Maxwell RSC Instrument from Promega

Product Specs
ItemMaxwell RSC Instrument
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS4500
QuantityEA
Processing Time30 to 60 min (depending on sample type and method)
CapacityUp to 16
Dimensions(WxDxH) 330.2 x 345.2 x 299.7 mm (13 x 13.6 x 11.8 in)
UV LampAvg. lifetime approximately 6,000 hrs
Molecular Weight~85kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(681) Immuno-priming durvalumab with bevacizumab in HER2-negative advanced breast cancer: a pilot clinical trialBreast Cancer ResearchDecember 1, 2020Miguel Quintela-Fandino et al.formalin-fixed paraffin-embedded tissue samples was isolated using the Maxwell® RSC RNA FFPE kit (#AS1440, Promega) with the Maxwell® RSC Instrument (Promega) according to the manufacturer’s protocol. Sequencing libraries were prepared with the “QuantSeq

(682) Unique genomic features and prognostic value of COSMIC mutational signature 4 in lung adenocarcinoma and lung squamous cell carcinomaAnnals of Translational MedicineNovember 26, 2020Xiuyu Cai, Zhenghe Chen, Meiling Deng, Zhiyong Li, Qianchao Wu, Jinwang Wei, Chun Dai, Guan Wang, Chun Luothe Maxwell RSC Blood DNA Kit (cat# AS1400, Promega, Madison, WI, USA) on a Maxwell RSC system (cat# AS4500, Promega). For formalin-fixed, paraffin-embedded (FFPE) tissue, DNA was extracted using the MagMAX FFPE DNA/RNA Ultra Kit

(683) Paired Rheumatoid Arthritis Synovial Biopsies From Small and Large Joints Show Similar Global Transcriptomic Patterns With Enrichment of Private Specificity TCRB and TCR Signaling PathwaysFrontiers in ImmunologyNovember 23, 2020Clement Triaille, Louise Vansteenkiste, Manuel Constant, JÃ©rÃ´me Ambroise, Laurent MÃ©ric de Bellefon, Adrien Nzeusseu Toukap, Tatiana Sokolova, Christine Galant, Pierre Coulie, Javier Carrasco, et al.Sequencing Total RNA was extracted from whole blood samples using Maxwell RSC simplyRNA blood kits (Promega) on a Maxwell RSC Instrument (Promega). Total RNA (250 ng for synovial samples and 250 to 1,088 ng for blood samples) was engaged

(684) Seroconversion and Skin Mucosal Parameters during Koi Herpesvirus Shedding in Common Carp, Cyprinus carpioInternational Journal of Molecular SciencesNovember 11, 2020Irene Cano, Brian Mulhearn, Sabiha Akter, Richard PaleyViral nucleocapsid was then extracted using the RSC Viral Total Nucleic Acid Purification Kit in a Maxwell® RSC Instrument (Promega, Southampton, UK). KHV orf90 gene was detected using the diagnostic Taqman qPCR described above,

(685) High Dose Ifosfamide in Relapsed and Unresectable High-Grade Osteosarcoma Patients: A Retrospective SeriesCellsOctober 31, 2020Emanuela Palmerini et al.slices and nucleic acids were extracted by Maxwell RSC DNA FFPE Kit and Maxwell RSC RNA FFPE Kit using a Maxwell RSC Instrument (Promega, Madison, WI, United States), according to manufacturer’s instructions. DNA and RNA were quantified

(686) NSDHL Frameshift Deletion in a Mixed Breed Dog with Progressive Epidermal NeviGenes (Basel)October 30, 2020Matthias Christen, Michaela Austel, Frane Banovic, Vidhya Jagannathan, Tosso LeebExtraction Genomic DNA was isolated from EDTA blood samples with the Maxwell RSC Whole Blood DNA Kit using a Maxwell RSC instrument (Promega, Dübendorf, Switzerland). Total RNA was extracted from skin biopsies using the RNeasy Mini

(687) Genome Characterization of a Novel Wastewater Bacteroides fragilis Bacteriophage (vB_BfrS_23) and its Host GB124Frontiers in MicrobiologyOctober 23, 2020Mohammad A. Tariq, Fiona Newberry, Rik Haagmans, Catherine Booth, Tom Wileman, Lesley Hoyles, Martha R. J. Clokie, James Ebdon, Simon R. CardingRSC Cultured Cell DNA Kit (AS1620) protocol (FB211) and run on the Promega Maxwell RSC Instrument (AS4500). phase (OD620 0.3–0.33) prior to incorporation into double BPRM agar overlays (Ebdon et al., 2007).

(688) A Novel HPLC-Based Method to Investigate on RNA after FixationInternational Journal of Molecular SciencesOctober 13, 2020Paolo Fattorini, Cristina Forzato, Domenico Tierno, Eleonora De Martino, Eros Azzalini, Vincenzo Canzonieri, Giorgio Stanta, Serena Bonin2008). RNA was isolated from four 10 µm-thick sections of fixed and paraffin-embedded specimens using the Maxwell® RSC instrument (Promega; Madison, WI, USA) following the instruction of the Maxwell® RSC RNA FFPE kit (Cat. No. AS1440).

(689) Use of a Hybrid Adeno-Associated Viral Vector Transposon System to Deliver the Insulin Gene to Diabetic NOD MiceCellsOctober 2, 2020Que T. La, Binhai Ren, Grant J. Logan, Sharon C. Cunningham, Neeta Khandekar, Najah T. Nassif, Bronwyn A. Oâ€™Brien, Ian E. Alexander, Ann M. Simpsontissues were obtained from NOD mice that did not develop diabetes. Total RNA was extracted using the MaxWell®RSC instrument and the MaxWell®RSC Simply RNA Tissue Kit (Promega, Madison, WI, USA). RNA samples were treated with

(690) Lower DNA methylation levels in CpG island shores of CR1, CLU, and PICALM in the blood of Japanese Alzheimerâ€™s disease patientsPLOS ONESeptember 29, 2020Risa Mitsumori, Kazuya Sakaguchi, Daichi Shigemizu, Taiki Mori, Shintaro Akiyama, Kouichi Ozaki, Shumpei Niida, Nobuyoshi Shimodagenomic DNA of 293 subjects were extracted from peripheral blood leukocytes by standard protocols using Maxwell RSC Instrument (Maxwell RSC Buffy Coat DNA Kit, Promega, PLOS ONE | https://doi.org/10.1371/journal.pone.0239196 September

(691) An NMR-based approach reveals the core structure of the functional domain of SINEUP lncRNAsNucleic acids researchSeptember 18, 2020Ohyama T, Takahashi H, Sharma H, Yamazaki T, Gustincich S, Ishii Y, Carninci P.RT-PCR Total RNA was extracted by using a Maxwell RSC simplyRNA Cells Kit (Promega, Fitchburg, WI, USA) and Maxwell RSC Instrument in accordance with the manufacturer's protocol. Total RNA (500 ng) was reverse transcribed to cDNA by

(692) Analysis of major otosclerosis-associated variants in RELN and TGFB1 genes in Polish patientsArchives of Medical ScienceSeptember 16, 2020Dominika OziÄ™bÅ‚o, Sara DomagaÅ‚a, Marcin L. Leja, Henryk SkarÅ¼yÅ„ski, Monika OÅ‚dakfrom whole blood samples or buccal swabs using a standard salting out procedure or automated method (Maxwell RSC Instrument, Promega, Germany), respectively. Three SNPs located in the RELN gene (rs39335, rs39350, rs39374) and

(693) Isolation and Characterization of Two Novel Colorectal Cancer Cell Lines, Containing a Subpopulation with Potential Stem-Like Properties: Treatment Options by MYC/NMYC InhibitionCancersSeptember 10, 2020Schulte Am Esch JSA, Windmöller BA, Hanewinkel J, Storm J, Förster C, Wilkens L, Krüger M, Kaltschmidt B, Kaltschmidt C.mutation analysis was performed using automatically DNA-extraction with Maxwell® RSC DNA FFPE Kit and the Maxwell® RSC instrument (Promega, Walldorf, Germany) according to the manufacturers guidelines in the Institute of Pathology

(694) LAMB3 Missense Variant in Australian Shepherd Dogs with Junctional Epidermolysis BullosaGenes (Basel)September 7, 2020Sarah Kiener, Aurore Laprais, Elizabeth A. Mauldin, Vidhya Jagannathan, Thierry Olivry, Tosso Leebfrom formalin-fixed paraffin-embedded (FFPE) tissue samples with the Maxwell RSC DNA FFPE Kit using a Maxwell RSC instrument (Promega, Dübendorf, Switzerland). 2.3. Histopathological Examinations Skin punch biopsies (8 mm) were

(695) Dietary α-Linolenic Acid Counters Cardioprotective Dysfunction in Diabetic Mice: Unconventional PUFA ProtectionNutrientsSeptember 2, 2020Russell JS, Griffith TA, Naghipour S, Vider J, Du Toit EF, Patel HH, Peart JN, Headrick JP.using the Maxwell® RSC simplyRNA Tissue Kit (Promega Corporation, Alexandria, NSW, Australia) on the Maxwell® RSC instrument (Promega Corporation, Alexandria, NSW, Australia), per manufacturer’s instructions. RNA was eluted into

(696) Recombinant thrombomodulin protects against LPS-induced acute respiratory distress syndrome via preservation of pulmonary endothelial glycocalyxBritish journal of pharmacologySeptember 1, 2020Suzuki K, Okada H, Takemura G, Takada C, Tomita H, Yano H, Muraki I, Zaikokuji R, Kuroda A, Fukuda H, et al.= 3 each). Total RNA was extracted using a simplyRNA Tissue Kit (Promega, Fitchburg, WI, USA) on a Maxwell RSC instrument. Gene expression analysis of the RNA samples was performed using an Agilent Expression Array (SurePrint

(697) A New ERAP2/Iso3 Isoform Expression Is Triggered by Different Microbial Stimuli in Human Cells. Could It Play a Role in the Modulation of SARS-CoV-2 Infection?CellsAugust 24, 2020Saulle I, Vanetti C, Goglia S, Vicentini C, Tombetti E, Garziano M, Clerici M, Biasin M.Vacutainer tubes containing EDTA (BD Vacutainer, San Diego, CA, USA). Total DNA was extracted by DNA purification Maxwell® RSC Instrument (Promega, Fitchburg, WI, USA) and quantified using the Nanodrop 2000 Instrument (Thermo Scientific,

(698) Distinct Mutation Patterns Reveal Melanoma Subtypes and Influence Immunotherapy Response in Advanced Melanoma PatientsCancers (Basel)August 20, 2020Franz J. Hilke, Tobias Sinnberg, Axel Gschwind, Heike Niessner, German Demidov, Teresa Amaral, Stephan Ossowski, Irina Bonzheim, Martin RÃ¶cken, Olaf Riess, et al.was extracted from macrodissected 5µm paraffin sections using the Maxwell® RSC DNA FFPE Kit and the Maxwell® RSC Instrument (Promega, Madison, WI, USA) according to the manufacturer’s instructions. 2.2. Sequencing The sequencing

(699) Highly sensitive detection of SARS-CoV-2 RNA by multiplex rRT-PCR for molecular diagnosis of COVID-19 by clinical laboratoriesClinica Chimica ActaAugust 1, 2020Takayuki Ishige, Shota Murata, Toshibumi Taniguchi, Akiko Miyabe, Kouichi Kitamura, Kenji Kawasaki, Motoi Nishimura, Hidetoshi Igari, Kazuyuki Matsushitaextracted using a Maxwell RSC Viral Total Nucleic Acid Purification Kit (Promega, Madison, WI, USA) using a Maxwell RSC Instrument (Promega). Total nucleic acid was eluted in 50 μL of nuclease-free water. 2.4 Multiplex rRT-PCR A

(700) Draft Genome Sequences of Non-H 2 S-Producing Strains of Salmonella enterica Serovars Infantis, Enteritidis, Berta, and Kiambu in JapanMicrobiology Resource AnnouncementsJuly 23, 2020Hiroshi Asakura, Junko Sakata, Shiori Yamamoto, Shizunobu Igimiadded to the deep-well processing plate supplied in the kit, and DNA extraction was performed in a Maxwell RSC instrument (Promega) with default settings. The concentration and purity of the isolated DNA were checked with

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