Maxwell RSC Instrument from Promega

Product Specs
ItemMaxwell RSC Instrument
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS4500
QuantityEA
Processing Time30 to 60 min (depending on sample type and method)
CapacityUp to 16
Dimensions(WxDxH) 330.2 x 345.2 x 299.7 mm (13 x 13.6 x 11.8 in)
UV LampAvg. lifetime approximately 6,000 hrs
Molecular Weight~85kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(701) Gentamicin Adsorption onto Soil Particles Prevents Overall Short-Term Effects on the Soil Microbiome and ResistomeAntibiotics (Basel)February 15, 2021Concepcion Sanchez-Cid, Alexandre Guironnet, Laure Wiest, Emmanuelle Vulliet, Timothy M. VogelTE buffer. After heating at 37 °C for 30 min shaking at 800 rpm, the lysate was purified using the Maxwell RSC Instrument and the Maxwell RSC Whole Blood DNA Kit (Promega, Madison, WI, USA). 4.5. DNA Extraction from Soil

(702) Integrated Genomics Identifies miR-181/TFAM Pathway as a Critical Driver of Drug Resistance in MelanomaInternational Journal of Molecular SciencesFebruary 11, 2021Anna Barbato, Antonella Iuliano, Mariagrazia Volpe, Romina Dâ€™Alterio, Simona Brillante, Filomena Massa, Rossella De Cegli, Sabrina Carrella, Massimiliano Salati, Annapina Russo, et al.Maxwell RSC simplyRNA Cells (Glomax Discover System, Promega, Madison, WI, USA) and processed with Maxwell RSC Instrument (Glomax Discover System, Promega, Madison, WI, USA) following manufacturer’s instructions. Single-stranded

(703) Comparison and optimisation of microRNA extraction from the plasma of healthy pregnant womenMolecular Medicine ReportsFebruary 5, 2021Victoria L. Parker, Bryony F. Cushen, Eleftherios Gavriil, Benjamin Marshall, Sarah Waite, Allan Pacey, Paul R. Heathall of the sample lysate was transferred into well 1 of the cartridges. The Maxwell® RSC Instrument (AS4500; Promega) (45) instrument and the ‘RSC miRNA Tissue’ method was used to begin the automated purification

(704) Pathogenic Huntingtin Repeat Expansions in Patients with Frontotemporal Dementia and Amyotrophic Lateral SclerosisNeuronFebruary 3, 2021Dewan R, Chia R, Ding J, Hickman RA, Stein TD, Abramzon Y, Ahmed S, Sabir MS, Portley MK, Tucci A, et al.pre-processing Whole-genome sequencing Genomic DNA was extracted from whole blood or cerebellar brain tissue using a Maxwell RSC Instrument (Promega Corp., Madison, WI, USA). Fluorometric quantitation of the genomic DNA samples was performed

(705) Detection of genes positively selected in Cuban Anolis lizards that naturally inhabit hot and open areas and currently thrive in urban areasEcology and EvolutionFebruary 1, 2021Shunsuke Kanamori, Antonio Cádiz, Luis M. Díaz, Yuu Ishii, Takuro Nakayama, Masakado KawataMini Kit (Qiagen, Hilden, Germany) or a Maxwell RSC Plant RNA Kit with the Maxwell® RSC Instrument AS4500 (Promega, Madison, WI, USA) in accordance with the manufacturers’ protocols. RNA concentrations of the

(706) Evaluating the Anti-Inflammatory and Antioxidant Effects of Broccoli Treated with High Hydrostatic Pressure in Cell ModelsFoodsJanuary 15, 2021Yi-Yuan Ke, Yuan-Tay Shyu, Sz-Jie WuMaxwell® RSC simplyRNA Cells Kit (Promega Co., Madison, WI, USA). Total RNA was extracted using the Maxwell® RSC Instrument. 2.9.2. RT-PCR Analysis Extracted RNA was reverse transcribed into cDNA using GoScriptTM Reverse Transcription

(707) Precision long-read metagenomics sequencing for food safety by detection and assembly of Shiga toxin-producing Escherichia coli in irrigation waterPLOS ONEJanuary 14, 2021Meghan Maguire, Julie A. Kase, Dwayne Roberson, Tim Muruvanda, Eric W. Brown, Marc Allard, Steven M. Musser, Narjol González-Escalonaof each spiked enrichment sample was extracted using the Maxwell RSC Cultured Cells DNA kit with a Maxwell RSC Instrument (Promega Corporation, Madison, WI) according to manufacturer’s instructions for Gram-negative bacteria

(708) Shorter Treatment-NaÃ¯ve Leukocyte Telomere Length is Associated with Poorer Overall Survival of Patients with Pancreatic Ductal AdenocarcinomaCancer Epidemiology, Biomarkers & PreventionJanuary 1, 2021Samuel O. Antwi, William R. Bamlet, Richard M. Cawthon, Kari G. Rabe, Brooke R. Druliner, Hugues Sicotte, Aminah Jatoi, Amit Mahipal, Lisa A. Boardman, Ann L. Oberg, et al.plasma, serum, and leukocytes (buffy coat). DNA was extracted from buffy coat leukocytes using the Maxwell RSC Instrument with appropriate kit (Promega) and quantitated with a Qubit fluorometer. LTL was measured by real-time

(709) Diagnosis of COVID-19 Based on Symptomatic Analysis of Hospital Healthcare Workers in Belgium: Observational Study in a Large Belgian Tertiary Care Center During Early COVID-19 OutbreakJournal of Occupational & Environmental MedicineJanuary 1, 2021Nele Van Loon, Mathieu Verbrugghe, Reinoud Cartuyvels, Dirk Ramaekersto each sample. Nucleic acid extraction was done with the Maxwell RSC Viral TNA kit (Promega) on the Maxwell RSC Instrument (Promega) according to the manufacturers’ instructions. An in-house rRT-PCR, based on the CDC oligonucleotide

(710) Weak-evidence Fusion Candidates Detected by a FusionPlex Assay Using the Ion Torrent SystemIn VivoJanuary 1, 2021SHIRO FUJITA, KATSUHIRO MASAGO, EIICHI SASAKI, SATOSHI TSUKUSHI, YOSHITSUGU HORIO, HIROAKI KURODA, TOYOAKI HIDAfresh-frozen samples. Total RNA was extracted from FFPE sections using the Maxwell RSC RNA FFPE Kit and Maxwell RSC Instrument (Promega, Madison, WI, USA). RNA extraction from fresh-frozen samples was performed using the RNeasy

(711) Magnetic beads combined with carbon black-based screen-printed electrodes for COVID-19: A reliable and miniaturized electrochemical immunosensor for SARS-CoV-2 detection in salivaBiosensors and BioelectronicsJanuary 1, 2021Laura Fabiani, Marco Saroglia, Giuseppe GalatÃ , Riccardo De Santis, Silvia Fillo, Vincenzo Luca, Giovanni Faggioni, Nino D'Amore, Elisa Regalbuto, Piero Salvatori, et al.extracted from 300 μL of swab's medium using a Maxwell RSC Viral Total Nucleic Acid Purification Kit on Maxwell RSC Instrument (Promega, USA). Total nucleic acid was eluted in a final volume of 50 μL of nuclease-free water. Then,

(712) Unsupervised Analysis of Flow Cytometry Data in a Clinical Setting Captures Cell Diversity and Allows Population DiscoveryFrontiers in immunologyJanuary 1, 2021Baumgaertner P, Sankar M, Herrera F, Benedetti F, Barras D, Thierry AC, Dangaj D, Kandalaft LE, Coukos G, Xenarios I, et al.(CD123+CD11c-CD4+HLA-DR+) and monocytes (CD14+) were sorted and RNA extraction was done with the Promega Maxwell RSC instrument using the Maxwell RSC simply RNA cell extraction kit (Promega AS 1390) following the manufacturer instructions.

(713) Insulin-Like Growth Factor 2 mRNA Binding Protein 3 Promotes Cell Proliferation of Malignant Mesothelioma Cells by Downregulating p27(Kip1)Frontiers in oncologyJanuary 1, 2021Endo I, Amatya VJ, Kushitani K, Kambara T, Nakagiri T, Fujii Y, Takeshima Y.6-well plates for 72 h. RNA was extracted from the cells using Maxwell RSC RNA Cells Kits using the Maxwell RSC Instrument (Promega KK, Tokyo, Japan) according to the manufacturer’s protocols. The extracted RNA was reverse

(714) Optimizing direct RT-LAMP to detect transmissible SARS-CoV-2 from primary nasopharyngeal swab samplesPLOS ONEDecember 31, 2020Dawn M. Dudley, Christina M. Newman, Andrea M. Weiler, Mitchell D. Ramuta, Cecilia G. Shortreed, Anna S. Heffron, Molly A. Accola, William M. Rehrauer, Thomas C. Friedrich, David H. O’Connorsamples arrived in our laboratory. RNA was isolated using the Viral Total Nucleic Acid kit for the Maxwell RSC instrument (Promega, Madison, WI) following the manufacturer’s instructions. Viral load quantification was performed

(715) Essential Oils as Alternative Biocides for the Preservation of Waterlogged Archaeological WoodMicroorganismsDecember 16, 2020Federica Antonelli et al.kit Maxwell® RSC Plant DNA Kit (AS1490, Promega) and total genomic microbial DNA was extracted with Maxwell® RSC Instrument (Promega). V3-V4 region of 16S rRNA gene (amplified using the primers described in Illumina 16S protocol:

(716) Profiling microRNAs in uncomplicated pregnancies: Serum vs. plasmaBiomedical ReportsDecember 14, 2020Victoria L Parker, Eleftherios Gavriil, Benjamin Marshall, Allan Pacey, Paul R Heathsample lysate was transferred into well 1 of the cartridges. The Maxwell® RSC Instrument (cat. no. AS4500: Promega Corporation) (59) instrument and the ‘RSC miRNA Tissue' method (60) was used to initiate the

(717) Genomic targets for high-resolution inference of kinship, ancestry and disease susceptibility in orang-utans (genus: Pongo)BMC GenomicsDecember 7, 2020Graham L. Banes, Emily D. Fountain, Alyssa Karklus, Hao-Ming Huang, Nian-Hong Jang-Liaw, Daniel L. Burgess, Jennifer Wendt, Cynthia Moehlenkamp, George F. Mayhewor Tissue DNA kits, respectively, as automated on the Banes et al. BMC Genomics (2020) 21:873 Maxwell RSC instrument (Promega). We then used the Multi-Ethnic Global Array (MEGA) chip (Illumina), having used BLAST to compare

(718) A COL7A1 Variant in a Litter of Neonatal Basset Hounds with Dystrophic Epidermolysis BullosaGenes (Basel)December 4, 2020Teresa Maria Garcia, Sarah Kiener, Vidhya Jagannathan, Duncan S. Russell, Tosso LeebMaxwell RSC Tissue DNA Kit or from ETDA blood samples with the Maxwell RSC Whole Blood Kit using a Maxwell RSC instrument (Promega, Dübendorf, Switzerland). 2.5. Whole Genome Sequencing An Illumina TruSeq PCR-free DNA library

(719) Time course of altered DNA methylation evoked by critical illness and by early administration of parenteral nutrition in the paediatric ICUClinical EpigeneticsDecember 1, 2020Ines Verlinden, Fabian GÃ¼iza, Inge Derese, Pieter J. Wouters, Koen Joosten, Sascha C. Verbruggen, Greet Van den Berghe, Ilse Vanhorebeekextracted from the blood cell samples and bisulphite-converted. DNA extractions were performed with the Maxwell®RSC instrument and the corresponding blood DNA purification kit (Promega Benelux b.v., Leiden, The Netherlands). All

(720) Development of genetic tools for the thermophilic filamentous fungus Thermoascus aurantiacusBiotechnology for BiofuelsDecember 1, 2020Raphael Gabriel, Julia Prinz, Marina Jecmenica, Carlos Romero-Vazquez, Pallas Chou, Simon Harth, Lena Floerl, Laure Curran, Anne Oostlander, Linda Matz, et al.uracil. For strain verification, the mycelium DNA was extracted with the Maxwell RSC Plant DNA Kit on the Maxwell RSC Instrument (Promega, Maidson, WI, USA) according to the manual. One modification involved bead beating of intact

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