Maxwell RSC Instrument from Promega

Product Specs
ItemMaxwell RSC Instrument
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS4500
QuantityEA
Processing Time30 to 60 min (depending on sample type and method)
CapacityUp to 16
Dimensions(WxDxH) 330.2 x 345.2 x 299.7 mm (13 x 13.6 x 11.8 in)
UV LampAvg. lifetime approximately 6,000 hrs
Molecular Weight~85kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(661) Diagnosis of COVID-19 Based on Symptomatic Analysis of Hospital Healthcare Workers in Belgium: Observational Study in a Large Belgian Tertiary Care Center During Early COVID-19 OutbreakJournal of Occupational & Environmental MedicineJanuary 1, 2021Nele Van Loon, Mathieu Verbrugghe, Reinoud Cartuyvels, Dirk Ramaekersto each sample. Nucleic acid extraction was done with the Maxwell RSC Viral TNA kit (Promega) on the Maxwell RSC Instrument (Promega) according to the manufacturers’ instructions. An in-house rRT-PCR, based on the CDC oligonucleotide

(662) Weak-evidence Fusion Candidates Detected by a FusionPlex Assay Using the Ion Torrent SystemIn VivoJanuary 1, 2021SHIRO FUJITA, KATSUHIRO MASAGO, EIICHI SASAKI, SATOSHI TSUKUSHI, YOSHITSUGU HORIO, HIROAKI KURODA, TOYOAKI HIDAfresh-frozen samples. Total RNA was extracted from FFPE sections using the Maxwell RSC RNA FFPE Kit and Maxwell RSC Instrument (Promega, Madison, WI, USA). RNA extraction from fresh-frozen samples was performed using the RNeasy

(663) Magnetic beads combined with carbon black-based screen-printed electrodes for COVID-19: A reliable and miniaturized electrochemical immunosensor for SARS-CoV-2 detection in salivaBiosensors and BioelectronicsJanuary 1, 2021Laura Fabiani, Marco Saroglia, Giuseppe GalatÃ , Riccardo De Santis, Silvia Fillo, Vincenzo Luca, Giovanni Faggioni, Nino D'Amore, Elisa Regalbuto, Piero Salvatori, et al.extracted from 300 μL of swab's medium using a Maxwell RSC Viral Total Nucleic Acid Purification Kit on Maxwell RSC Instrument (Promega, USA). Total nucleic acid was eluted in a final volume of 50 μL of nuclease-free water. Then,

(664) Unsupervised Analysis of Flow Cytometry Data in a Clinical Setting Captures Cell Diversity and Allows Population DiscoveryFrontiers in immunologyJanuary 1, 2021Baumgaertner P, Sankar M, Herrera F, Benedetti F, Barras D, Thierry AC, Dangaj D, Kandalaft LE, Coukos G, Xenarios I, et al.(CD123+CD11c-CD4+HLA-DR+) and monocytes (CD14+) were sorted and RNA extraction was done with the Promega Maxwell RSC instrument using the Maxwell RSC simply RNA cell extraction kit (Promega AS 1390) following the manufacturer instructions.

(665) Insulin-Like Growth Factor 2 mRNA Binding Protein 3 Promotes Cell Proliferation of Malignant Mesothelioma Cells by Downregulating p27(Kip1)Frontiers in oncologyJanuary 1, 2021Endo I, Amatya VJ, Kushitani K, Kambara T, Nakagiri T, Fujii Y, Takeshima Y.6-well plates for 72 h. RNA was extracted from the cells using Maxwell RSC RNA Cells Kits using the Maxwell RSC Instrument (Promega KK, Tokyo, Japan) according to the manufacturer’s protocols. The extracted RNA was reverse

(666) Optimizing direct RT-LAMP to detect transmissible SARS-CoV-2 from primary nasopharyngeal swab samplesPLOS ONEDecember 31, 2020Dawn M. Dudley, Christina M. Newman, Andrea M. Weiler, Mitchell D. Ramuta, Cecilia G. Shortreed, Anna S. Heffron, Molly A. Accola, William M. Rehrauer, Thomas C. Friedrich, David H. O’Connorsamples arrived in our laboratory. RNA was isolated using the Viral Total Nucleic Acid kit for the Maxwell RSC instrument (Promega, Madison, WI) following the manufacturer’s instructions. Viral load quantification was performed

(667) Essential Oils as Alternative Biocides for the Preservation of Waterlogged Archaeological WoodMicroorganismsDecember 16, 2020Federica Antonelli et al.kit Maxwell® RSC Plant DNA Kit (AS1490, Promega) and total genomic microbial DNA was extracted with Maxwell® RSC Instrument (Promega). V3-V4 region of 16S rRNA gene (amplified using the primers described in Illumina 16S protocol:

(668) Profiling microRNAs in uncomplicated pregnancies: Serum vs. plasmaBiomedical ReportsDecember 14, 2020Victoria L Parker, Eleftherios Gavriil, Benjamin Marshall, Allan Pacey, Paul R Heathsample lysate was transferred into well 1 of the cartridges. The Maxwell® RSC Instrument (cat. no. AS4500: Promega Corporation) (59) instrument and the ‘RSC miRNA Tissue' method (60) was used to initiate the

(669) Genomic targets for high-resolution inference of kinship, ancestry and disease susceptibility in orang-utans (genus: Pongo)BMC GenomicsDecember 7, 2020Graham L. Banes, Emily D. Fountain, Alyssa Karklus, Hao-Ming Huang, Nian-Hong Jang-Liaw, Daniel L. Burgess, Jennifer Wendt, Cynthia Moehlenkamp, George F. Mayhewor Tissue DNA kits, respectively, as automated on the Banes et al. BMC Genomics (2020) 21:873 Maxwell RSC instrument (Promega). We then used the Multi-Ethnic Global Array (MEGA) chip (Illumina), having used BLAST to compare

(670) A COL7A1 Variant in a Litter of Neonatal Basset Hounds with Dystrophic Epidermolysis BullosaGenes (Basel)December 4, 2020Teresa Maria Garcia, Sarah Kiener, Vidhya Jagannathan, Duncan S. Russell, Tosso LeebMaxwell RSC Tissue DNA Kit or from ETDA blood samples with the Maxwell RSC Whole Blood Kit using a Maxwell RSC instrument (Promega, Dübendorf, Switzerland). 2.5. Whole Genome Sequencing An Illumina TruSeq PCR-free DNA library

(671) Analysis of SARS-CoV-2 vertical transmission during pregnancyNature CommunicationsDecember 1, 2020Claudio Fenizia, Mara Biasin, Irene Cetin, Patrizia Vergani, Davide Mileto, Arsenio Spinillo, Maria Rita Gismondo, Francesca Perotti, Clelia Callegari, Alessandro Mancon, et al.as plasma samples from funicular blood and placental tissues were included. RNA was extracted by the Maxwell® RSC Instrument with the Maxwell® RSC Viral Total Nucleic Acid Puriﬁcation Kit (Promega, Fitchburg, WI, USA). As a result,

(672) Development of genetic tools for the thermophilic filamentous fungus Thermoascus aurantiacusBiotechnology for BiofuelsDecember 1, 2020Raphael Gabriel, Julia Prinz, Marina Jecmenica, Carlos Romero-Vazquez, Pallas Chou, Simon Harth, Lena Floerl, Laure Curran, Anne Oostlander, Linda Matz, et al.uracil. For strain verification, the mycelium DNA was extracted with the Maxwell RSC Plant DNA Kit on the Maxwell RSC Instrument (Promega, Maidson, WI, USA) according to the manual. One modification involved bead beating of intact

(673) Time course of altered DNA methylation evoked by critical illness and by early administration of parenteral nutrition in the paediatric ICUClinical EpigeneticsDecember 1, 2020Ines Verlinden, Fabian GÃ¼iza, Inge Derese, Pieter J. Wouters, Koen Joosten, Sascha C. Verbruggen, Greet Van den Berghe, Ilse Vanhorebeekextracted from the blood cell samples and bisulphite-converted. DNA extractions were performed with the Maxwell®RSC instrument and the corresponding blood DNA purification kit (Promega Benelux b.v., Leiden, The Netherlands). All

(674) A new mouse line with reduced GluA2 Q/R site RNA editing exhibits loss of dendritic spines, hippocampal CA1-neuron loss, learning and memory impairments and NMDA receptor-independent seizure vulnerabilityMolecular BrainDecember 1, 2020Lyndsey M. Konen, Amanda L. Wright, Gordon A. Royle, Gary P. Morris, Benjamin K. Lau, Patrick W. Seow, Raphael Zinn, Luke T. Milham, Christopher W. Vaughan, Bryce Visselrequired. Total RNA was isolated using a Maxwell® RSC simplyRNA Tissue Kit (Promega, Cat# AS1340) and a Maxwell® RSC Instrument (Promega), according to the manufacturer’s instructions. As part of the protocol a DNAse treatment step

(675) E-cadherin to P-cadherin switching in lobular breast cancer with tubular elementsModern pathology : an official journal of the United States and Canadian Academy of Pathology, IncDecember 1, 2020Christgen M, Bartels S, van Luttikhuizen JL, Bublitz J, Rieger LU, Christgen H, Stark H, Sander B, Lehmann U, Steinemann D, et al.well. Next, genomic DNA was extracted with the Maxwell RSC DNA FFPE kit (Promega, Madison, USA) on a Maxwell RSC instrument (Promega) according to the manufacturer’s recommendations. DNA was quantified using a Qubit 2.0 fluorometer

(676) Narcea—an unknown, ancient cultivated rose variety from northern SpainHorticulture ResearchDecember 1, 2020María-Carmen Martínez et al.CTAB buffer, as described by De la Rosa et al.28. DNA was then extracted using the Maxwell® PureFood Kit and Maxwell RSC Instrument (Promega Corporation, Madison, WI, USA) following the manufacture’s recommendations. The extracted DNA

(677) Sequencing identifies multiple early introductions of SARS-CoV-2 to the New York City regionGenome ResearchDecember 1, 2020Matthew T. Maurano et al.using two platforms for parallel sample processing: 1. By using the Maxwell RSC instrument (Promega AS4500), total RNA was extracted from 300 µL of VTM with the buccal swab DNA kit (Promega AS1640). The following

(678) Vertical transmission of SARS-CoV-2 infection and preterm birthEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical MicrobiologyDecember 1, 2020Pulinx B, Kieffer D, Michiels I, Petermans S, Strybol D, Delvaux S, Baldewijns M, Raymaekers M, Cartuyvels R, Maurissen W.sample. Nucleic acid extraction was done with the Maxwell RSC Viral TNA kit (Promega, Madison, WI) on the Maxwell RSC Instrument (Promega, Madison, WI) according to the manufacturers’ instructions. Our in-house rRT-PCR is based on

(679) Genetic Characterization of Streptococcus pyogenes emm89 Strains Isolated in Japan From 2011 to 2019Infectious Microbes and DiseasesDecember 1, 2020Yujiro Hirose, Masaya Yamaguchi, Norihiko Takemoto, Tohru Miyoshi-Akiyama, Tomoko Sumitomo, Masanobu Nakata, Tadayoshi Ikebe, Tomoki Hanada, Takahiro Yamaguchi, Ryuji Kawahara, et al.0.5 mg/mL achromopeptidase (Wako), and genomic DNA was extracted from overnight cell cultures using a Maxwell® RSC instrument with a Maxwell® RSC PureFood GMO and Authentication Kit (Promega Corporation, Madison, WI, USA). Paired-end

(680) SARS-CoV-2 surveillance for a non-human primate breeding research facilityJournal of medical primatologyDecember 1, 2020Yee JL, Van Rompay KKA, Carpenter AB, Nham PB, Halley BM, Iyer SS, Hartigan-O'Connor DJ, Miller CJ, Roberts JA.and/or nasopharyngeal swabs using viral total nucleic acid purification reagents on the semi‐automated Maxwell RSC Instrument (Promega) following the manufacturer's instructions. 200 µL of swab eluate was lysed and proteinase

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