Maxwell RSC Instrument from Promega

Product Specs
ItemMaxwell RSC Instrument
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS4500
QuantityEA
Processing Time30 to 60 min (depending on sample type and method)
CapacityUp to 16
Dimensions(WxDxH) 330.2 x 345.2 x 299.7 mm (13 x 13.6 x 11.8 in)
UV LampAvg. lifetime approximately 6,000 hrs
Molecular Weight~85kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(661) Long-Term Grow-Out Affects Campylobacter jejuni Colonization Fitness in Coincidence With Altered Microbiota and Lipid Composition in the Cecum of Laying HensFrontiers in Veterinary ScienceJune 18, 2021Hiroshi Asakura, Tatsuya Nakayama, Shiori Yamamoto, Kazuki Izawa, Jun Kawase, Yasushi Torii, Satoshi Murakamiμl of lysis buffer (Promega). DNA extraction was then carried out using a Maxwell Blood DNA kit in a Maxwell RSC instrument (Promega). The concentration and quality of the extracted DNA were measured on a Tape Station 4150 system

(662) Precise transcriptional control of cellular quiescence by BRAVO/WOX5 complex in Arabidopsis rootsMolecular Systems BiologyJune 16, 2021Isabel BetegÃ³nâ€Putze, Josep Mercadal, Nadja Bosch, Ainoa Planasâ€Riverola, Mar MarquÃ¨sâ€Bueno, Josep Vilarrasaâ€Blasi, David Frigola, Rebecca C Burkart, Cristina MartÃnez, Ana Conesa, et al.assay RNA was extracted from root tip tissue with the Maxwell® RSC Plant RNA Kit (Promega) using the Maxwell® RSC instrument (Promega) according to the manufacturer's recommendations, and concentrations were checked using NanoDrop

(663) Genetic Characterization of Cancer of Unknown Primary Using Liquid Biopsy ApproachesFrontiers in Cell and Developmental BiologyJune 10, 2021Noemi Laprovitera et al.FoundationOne CDx (F1CDx) assay. ccfDNA was purified from 1 ml of plasma by Maxwell RSC instrument (Cat No: AS4500, Promega, Madison, WI, United States) using the Maxwell RSC ccfDNA Plasma Kit (Cat No: AS1480, Promega).

(664) Macrophagic Inflammatory Response Next to Dental Implants with Different Macro- and Micro-Structure: An In Vitro StudyApplied SciencesJune 8, 2021Maria Menini, Francesca Delucchi, Domenico Baldi, Francesco Pera, Francesco Bagnasco, Paolo Pesce(Promega, Madison, WI, USA) following the supplier’s instructions and the nucleic acid automated extractor Maxwell® RSC instrument (Promega). RNAs of Appl. Sci. 2021, 11, 5324 4 of 10 extracted samples at 4 h, eluted in H2O,

(665) SARS-CoV-2 Entry: At the Crossroads of CD147 and ACE2CellsJune 8, 2021Fenizia C, Galbiati S, Vanetti C, Vago R, Clerici M, Tacchetti C, Daniele T.Maxwell® RSC Viral Total Nucleic Acid Purification Kit was used to extract RNA from 250 µL of cell culture supernatants employing the Maxwell® RSC Instrument (Promega, Madison, WI, USA). The remaining supernatant was conveniently stored at −80 ◦ C for the TCID50

(666) Modeling plasticity and dysplasia of pancreatic ductal organoids derived from human pluripotent stem cellsCell stem cellJune 3, 2021Breunig M, Merkle J, Wagner M, Melzer MK, Barth TFE, Engleitner T, Krumm J, Wiedenmann S, Cohrs CM, Perkhofer L, et al.added and samples were incubated for 30 min at 65°C, cooled down on ice and further processed on a Maxwell RSC instrument according to manufacturer’s instructions. Eluted DNA was quantified using Qubit dsDNA HS reagents (Thermo

(667) Ethnic and trans-ethnic genome-wide association studies identify new loci influencing Japanese Alzheimerâ€™s disease riskTranslational PsychiatryJune 1, 2021Daichi Shigemizu, Risa Mitsumori, Shintaro Akiyama, Akinori Miyashita, Takashi Morizono, Sayuri Higaki, Yuya Asanomi, Norikazu Hara, Gen Tamiya, Kengo Kinoshita, Takeshi Ikeuchi, Shumpei Niida, Kouichi Ozakiwas 1.37:1. Genomic DNA was extracted from peripheral blood leukocytes by standard protocols using a Maxwell RSC Instrument (Maxwell RSC Buffy Coat DNA Kit, Promega, USA). All subjects were of Japanese origin and provided informed

(668) Follow-up testing of borderline SARS-CoV-2 patients by rRT-PCR allows early diagnosis of COVID-19Diagnostic Microbiology and Infectious DiseaseJune 1, 2021Joost Boeckmans, Reinoud Cartuyvels, Petra Hilkens, Liesbeth Bruckers, Koen Magerman, Luc Waumans, Marijke Raymaekerssamples were analyzed with one of the following 3 methods:The first method included RNA extraction on the Maxwell RSC Instrument (Promega) using the RSC Viral TNA kit (Promega) according to the manufacturers’ instructions. An extraction

(669) Comparative analysis of post-transplant lymphoproliferative disorders after solid organ and hematopoietic stem cell transplantation reveals differences in the tumor microenvironmentVirchows ArchivJune 1, 2021Mathis Overkamp, Massimo Granai, Irina Bonzheim, Julia Steinhilber, Jens Schittenhelm, Wolfgang Bethge, Leticia Quintanilla-Martinez, Falko Fend, Birgit Federmannwas extracted from macrodissected 5-μm paraffin sections using the Maxwell® RSC DNA FFPE Kit and the Maxwell® RSC Instrument (Promega, Madison, WI, USA) according to the manufacturer’s instructions. The PCR for IGH gene rearrangements

(670) Detection of PIK3CA Gene Mutation in Head and Neck Squamous Cell Carcinoma Using Droplet Digital PCR and RT-qPCRBiomoleculesMay 31, 2021Edyta M. Borkowska, Magda Barańska, Magdalena Kowalczyk, Wioletta PietruszewskaMaxwell RSC DNA FFPE Kit (Promega Corporations, Madison, WI, USA, cat No AS1450) and automatic isolation on AS4500 device (Promega Corporations, Madison, WI, USA). The purity of extracted genomic DNA was checked by measuring

(671) Classification of molecular subtypes of high-grade serous ovarian cancer by MALDI-ImagingJournal of Clinical OncologyMay 20, 2021Wanja Nikolai Kassuhn et al.Classification by NanoString Technology Total RNA extraction was performed on FFPE tissue sections using the Maxwell RSC instrument by Promega (Promega GmbH, Walldorf, Germany) following the manufacturer’s instructions. Two 5 µm thick

(672) Digital Droplet PCR for SARS-CoV-2 Resolves Borderline CasesAmerican Journal of Clinical PathologyMay 18, 2021Jing Xu, Timothy Kirtek, Yan Xu, Hui Zheng, Huiyu Yao, Emily Ostman, Dwight Oliver, James S Malter, Jeffrey R Gagan, Jeffrey A SoRellenucleic acids from pooled or individual residual NP collections and controls were obtained using a Maxwell RSC instrument (Promega), with an offboard lysis protocol and the following volume parameters: 400 µL input, 500 µL

(673) Molecular Detection and Phylogeny of Tick-Borne Pathogens in Ticks Collected from Dogs in the Republic of KoreaPathogensMay 17, 2021A-Tai Truong, Jinhyeong Noh, Yeojin Park, Hyun-Ji Seo, Keun-Ho Kim, Subin Min, Jiyeon Lim, Mi-Sun Yoo, Heung-Chul Kim, Terry A. Klein, et al.Precellys 24 tissue homogenizer (Bertin Instruments, Montigny-le-Bretonux, France). The tick homogenate was placed in a Maxwell RSC instrument (Promega, Madison, WI, USA) according to the manufacturer’s instructions. The purification of the total

(674) Characterization of Plasma Cell-Free DNA Integrity Using Droplet-Based Digital PCR: Toward the Development of Circulating Tumor DNA-Dedicated AssaysFrontiers in OncologyMay 6, 2021Geoffroy Poulet et al.with the use of the Maxwell RSC ccfDNA Plasma kit (Promega; called Ext.kit 1 in the manuscript) using Maxwell® RSC Instrument. In the PLACOL study and corresponding control group, plasma from healthy individuals or mCRC patients

(675) Genetic Causes of Cardiomyopathy in Children: First Results From the Pediatric Cardiomyopathy Genes StudyJournal of the American Heart AssociationMay 4, 2021Stephanie M. Ware, James D. Wilkinson, Muhammad Tariq, Jeffrey A. Schubert, Arthi Sridhar, Steven D. Colan, Ling Shi, Charles E. Canter, Daphne T. Hsu, Steven A. Webber, et al.were collected at each site and shipped to the Genetic Coordinating Center. DNA was extracted on a Maxwell RSC instrument (Promega, Madison, WI) according to the manufacturer’s protocol. Exome sequencing was performed using sequence

(676) Chromosome 2q gain and epigenetic silencing of GATA3 in microglandular adenosis of the breastThe Journal of Pathology: Clinical ResearchMay 1, 2021Martin Radner, Jana Lisa van Luttikhuizen, Stephan Bartels, Janin Bublitz, Isabel Grote, Luisa Rieger, Henriette Christgen, Helge Stark, Christopher Werlein, Marcel Lafos, et al.and RNA were extracted with the Maxwell RSC DNA and RNA FFPE kits (Promega, Madison, WI,USA) using a Maxwell RSC instrument (Promega) according to the manufacturer's recommendations. DNA was quantified using a Qubit 2.0 fluorometer

(677) L2HGDH Missense Variant in a Cat with L-2-Hydroxyglutaric AciduriaGenes (Basel)May 1, 2021Matthias Christen, Nils Janzen, Anne Fraser, Adrian C. Sewell, Vidhya Jagannathan, Julien Guevar, Tosso Leeb, Daniel Sanchez-MasianDNA Extraction Genomic DNA was isolated from EDTA blood with the Maxwell RSC Whole Blood Kit using a Maxwell RSC instrument (Promega, Dübendorf, Switzerland). 2.7. Whole-Genome Sequencing An Illumina TruSeq PCR-free DNA library

(678) Cranial Mandibular Fibrosis Syndrome in Adult Farmed Rainbow Trout Oncorhynchus mykissPathogensApril 30, 2021Irene Cano, John Worswick, Brian Mulhearn, Matt Green, Stephen W. Feist, Morag Clintonsupernatant of RTG-2 cells showing CPEs using a Maxwell® RSC viral total nucleic acid purification kit in a Maxwell® RSC instrument (Promega, Southampton, UK) following the manufacturer’s instructions. Reverse transcription (RT) was

(679) MicroRNA Expression in Extracellular Vesicles from Nasal Lavage Fluid in Chronic RhinosinusitisBiomedicinesApril 26, 2021Seungbin Cha, Eun-Hye Seo, Seung Hyun Lee, Kyung Soo Kim, Chung-Sik Oh, Jong-Seok Moon, Jin Kook Kimusing the Promega Maxwell® RSC miRNA Tissue Kit (AS1460; Promega Corporation, Fitchburg, WI, USA) and Maxwell® RSC instrument (Promega Corporation, Madison, WI, USA) according to the manufacturer’s instructions. The concentration

(680) Isolation and Characterization of Phosphate Solubilizing Streptomyces sp. Endemic from Sugar Beet Fields of the Beni-Mellal Region in MoroccoMicroorganismsApril 24, 2021Yassine Aallam, Driss Dhiba, SanaÃ¢ Lemriss, Amal Souiri, Fatma Karray, Taoufik El Rasafi, Nezha SaÃ¯di, Abdelmajid Haddioui, SaÃ¢d El Kabbaj, Marie JoÃ«lle Virolle, et al.centrifugation (16,000× g for 10 min) and the mycelial pellet was used for automated extraction of DNA with the Maxwell® RSC Instrument (Promega, Madison, WI, USA) and the Maxwell® RSC PureFood GMO and Authentication Kit (Promega) according

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