Maxwell RSC Instrument from Promega

Product Specs
ItemMaxwell RSC Instrument
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS4500
QuantityEA
Processing Time30 to 60 min (depending on sample type and method)
CapacityUp to 16
Dimensions(WxDxH) 330.2 x 345.2 x 299.7 mm (13 x 13.6 x 11.8 in)
UV LampAvg. lifetime approximately 6,000 hrs
Molecular Weight~85kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(621) The splicing regulator SLU7 is required to preserve DNMT1 protein stability and DNA methylationNucleic acids researchSeptember 7, 2021Recalde M, Gárate-Rascón M, Elizalde M, Azkona M, Latasa MU, Bárcena-Varela M, Sangro B, Fernández-Barrena MG, Ávila MA, Arechederra M, et al.(University of Utah, USA). Total DNA isolation Total DNA from cell lines was extracted using the automated Maxwell RSC Instrument with cultured cells DNA kit (Promega, Madison, WI, USA). DNA purity and concentration were measured

(622) Serum Vitamin D Is Associated with Antioxidant Potential in Peri-Parturient CowsAntioxidants (Basel)September 6, 2021Jaimie M. Strickland, Lauren Wisnieski, Vengai Mavangira, Lorraine M. Sordilloperformed as previously described by our group [41]. The RNA from BAECs was extracted using the (Promega Maxwell RSC instrument, Madison, WI, USA) following the manufacturer’s protocol. Quantitative real-time PCR (qRT-PCR) was performed

(623) Saliva Testing Is Accurate for Early-Stage and Presymptomatic COVID-19Microbiology SpectrumSeptember 3, 2021Abigail J. Johnson et al.or the Promega Maxwell RSC viral total nucleic acid kit (number AS1330; Promega, Madison, WI) on the Maxwell RSC instrument per the manufacturer’s instructions; bridging studies showed equivalent limits of detection between

(624) Prediction of False-Positive Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Molecular Results in a High-Throughput Open-Platform SystemThe Journal of Molecular DiagnosticsSeptember 1, 2021Ryan J. Martinez, Nathan Pankratz, Matthew Schomaker, Jerry Daniel, Kenneth Beckman, Amy B. Karger, Bharat Thyagarajan, Patricia Ferreri, Sophia L. Yohe, Andrew C. Nelson16-sample throughput Promega Maxwell RSC Viral Total Nucleic Acid kit (Promega, Madison, WI) on the Maxwell RSC instrument; iii) the automated 96-sample throughput MagMAX Viral and Pathogen Nucleic Acid Isolation kit on the

(625) Screening for extremely rare pathogenic variants of monogenic diabetes using targeted panel sequencingEndocrineSeptember 1, 2021Tomasz PÅ‚oszaj, Karolina Antosik, Paulina Jakiel, Agnieszka ZmysÅ‚owska, Maciej Borowiecreaction Subjects DNA isolation was performed from peripheral blood with a semi-automatic device from the Promega Maxwell® RSC Instrument. The library was prepared in accordance with the Agilent SureSelectQXT Target Enrichment protocol using

(626) Association of Initial Viral Load in Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Patients with Outcome and SymptomsThe American Journal of PathologySeptember 1, 2021Kimon V. Argyropoulos, Antonio Serrano, Jiyuan Hu, Margaret Black, Xiaojun Feng, Guomiao Shen, Melissa Call, Min J. Kim, Andrew Lytle, Brendan Belovarac, et al.included, serving as the internal control. Viral RNA was extracted within using the Promega (Madison, WI) Maxwell RSC Instrument and Swab kit, according to the manufacturer's instructions. All quantitative RT-PCR assays were performed

(627) Monitoring levels of circulating cell‐free DNA in patients with metastatic colorectal cancer as a potential biomarker of responses to regorafenib treatmentMolecular OncologySeptember 1, 2021Brice Pastor, Thierry André, Julie Henriques, Isabelle Trouilloud, Christophe Tournigand, Marine Jary, Thibault Mazard, Christophe Louvet, Simon Azan, Audrey Bauer, et al.at −20 °C until use if not used immediately. Circulating cfDNA was also extracted from 1 mL plasma (Maxwell® RSC Instrument) using the cfDNA Plasma Kit (Promega Corporation, Madison, WI, USA) in an elution volume of 130 µL.

(628) Geographic Variations in Test Reactivity for the Serological Diagnosis of Trypanosoma cruzi InfectionJ Clin MicrobiolSeptember 1, 2021Carine Truyens, Eric Dumonteil, Jackeline Alger, Maria Luisa Cafferata, Alvaro Ciganda, Luz Gibbons, Claudia Herrera, Sergio Sosa-Estani, Pierre BuekensDNA was extracted from 300 μl of blood conserved in 6 M guanidine HCl, 0.2 M EDTA using an automated Maxwell RSC instrument (Promega Benelux, Leiden, The Netherland) as described previously (13). All DNA extractions and PCR assays

(629) A Missense Variant in SLC39A4 in a Litter of Turkish Van Cats with Acrodermatitis EnteropathicaGenes (Basel)August 25, 2021Sarah Kiener, Robert Cikota, Monika Welle, Vidhya Jagannathan, Susanne Ã…hman, Tosso Leebbreeds. Genomic DNA was isolated from ETDA blood samples with the Maxwell RSC Whole Blood Kit using a Maxwell RSC instrument (Promega, Dübendorf, Switzerland). 2.2. Histopathological Examinations Skin biopsies (6 mm punch and

(630) Molecular Detection and Phylogenetic Analysis of Tick-Borne Pathogens in Ticks Collected from Horses in the Republic of KoreaPathogensAugust 24, 2021Hyun-Ji Seo, A-Tai Truong, Keun-Ho Kim, Ji-Yeon Lim, Subin Min, Heung-Chul Kim, Mi-Sun Yoo, Soon-Seek Yoon, Terry A. Klein, Yun Sang Chotube. After incubating at 56 ℃ for 10 min purification of nucleic acids was done by using an automated Maxwell RSC Instrument (Promega, Madison, WI, USA). Isolated material was stored at −80 °C until further molecular analysis. 4.3.

(631) Variants Affecting the C-Terminal Tail of UNC93B1 Are Not a Common Risk Factor for Systemic Lupus ErythematosusGenes (Basel)August 19, 2021Sarah Kiener, Camillo Ribi, Irene Keller, Carlo Chizzolini, Marten Trendelenburg, Uyen Huynh-Do, Johannes von Kempis, Tosso on behalf of Swiss SLE Cohort Study (SSCS), Tosso on behalf of Swiss SLE Cohort Study (SSCS)[27,28,29]. Genomic DNA was isolated from ETDA blood samples with the Maxwell RSC Whole Blood Kit using a Maxwell RSC instrument (Promega, Dübendorf, Switzerland). 2.2. UNC93B1 Targeted Sanger Sequencing A 1000 bp PCR amplicon

(632) Co-occurring gain-of-function mutations in HER2 and HER3 modulate HER2/HER3 activation, oncogenesis, and HER2 inhibitor sensitivityCancer CellAugust 9, 2021Ariella B. Hanker et al.and ERBB3 RNA was isolated from CW2 cells using the Maxwell RSC simplyRNA Cells Kit (Promega) on the Maxwell RSC Instrument (Promega). RNA was isolated from SA493 organoids using the Qiagen RNeasy Micro Kit. Reverse transcription

(633) Whole genome sequencing of low input circulating cell‐free DNA obtained from normal human subjectsPhysiological ReportsAugust 4, 2021Julie F. Foley et al.(Maxwell® RSC ccfDNA Plasma Kit, Promega) and eluted with nuclease‐free water (50 μl volume) on a Promega Maxwell® RSC instrument. Sample yield was determined by a fluorometric method (Quantus™ Fluorometer, Promega). Fragmentation

(634) Deletion of the SELENOP gene leads to CNS atrophy with cerebellar ataxia in dogsPLOS GeneticsAugust 2, 2021Matthias Christen, Sandra HÃ¶gler, Miriam Kleiter, Michael Leschnik, Corinna Weber, Denise Thaller, Vidhya Jagannathan, Tosso Leebmethods using the Maxwell RSC Whole Blood DNA and the Maxwell RSC Blood DNA Kits in combination with the Maxwell RSC instrument (Promega, Dübendorf, Switzerland). Linkage analysis and homozygosity mapping Genotype data for the

(635) SARS-CoV-2 genomic surveillance in Costa Rica: Evidence of a divergent population and an increased detection of a spike T1117I mutationInfection, Genetics and EvolutionAugust 1, 2021Jose Arturo Molina-Mora, Estela Cordero-Laurent, Adriana GodÃnez, Melany CalderÃ³n-Osorno, Hebleen Brenes, Claudio Soto-Garita, Cristian PÃ©rez-Corrales, COINGESA-CR Consorcio Interinstitucional de Estudios GenÃ³micos del SARS-CoV-2 Costa Rica, Jan Felix Drexler, Andres Moreira-Soto, et al.in a QIACube extractor or with Maxwell® RSC Viral Total Nucleic Acid Purification Kit (Promega) in a Maxwell® RSC instrument following the manufacturer's protocol. cDNA for library preparation was then synthesized and purified

(636) Gene therapy with AR isoform 2 rescues spinal and bulbar muscular atrophy phenotype by modulating AR transcriptional activityScience advancesAugust 1, 2021Lim WF, Forouhan M, Roberts TC, Dabney J, Ellerington R, Speciale AA, Manzano R, Lieto M, Sangha G, Banerjee S, et al.was isolated from murine tissues or cell lines using Maxwell RSC simplyRNA tissue or cells kit and Maxwell RSC Instrument (Promega) according to the manufacturer’s protocol. One microgram of RNA was used for cDNA synthesis

(637) MiRNA Let-7a and Let-7d Are Induced by Globotriaosylceramide via NF-kB Activation in Fabry DiseaseGenes (Basel)July 30, 2021Nadine Maier, Constantin Gatterer, Patrick Haider, Manuel Salzmann, Christoph Kaun, Walter S. Speidl, Gere Sunder-Plassmann, Bruno K. Podesser, Johann Wojta, Senta Graf, et al.previously [16]. RNA from cell culture lysates was prepared using Maxwell RSC simplyRNA Tissue kits on a Maxwell RSC Instrument (both Promega, Madison, WI, USA). To create cDNA from the extracted RNA, 10 μL sample were added to

(638) SARS-CoV-2 Infected Pediatric Cerebral Cortical Neurons: Transcriptomic Analysis and Potential Role of Toll-like Receptors in PathogenesisInternational Journal of Molecular SciencesJuly 28, 2021Agnese Gugliandolo et al.effect. SARS-CoV-2 RNA was extracted from 1, 3 and 6 days post infection (dpi) supernatant using the Maxwell® RSC Instrument with Maxwell® RSC Viral Total Nucleic Acid Purification Kit (Promega, Fitchburg, WI, USA). Viral RNA

(639) Gene Expression Analysis Provides New Insights into the Mechanism of Intramuscular Fat Formation in Japanese Black CattleGenes (Basel)July 21, 2021Shuji Ueda, Mana Hosoda, Ken-ichi Yoshino, Minoru Yamanoue, Yasuhito Shirai°C. Total RNA was purified using ~100 mg of fat tissue by the Maxwell RSC simply RNA tissue kit using a Maxwell RSC instrument (Promega K. K., Tokyo, Japan) according to manufacturer instructions. The concentration of the obtained

(640) Comparative genomic analysis of Escherichia coli isolates from cases of bovine clinical mastitis identifies nine specific pathotype marker genesMicrobial GenomicsJuly 6, 2021Dongyun Jung, Soyoun Park, Janina Ruffini, Forest Dussault, Simon Dufour, Jennifer RonholmDNA from isolates that did not produce high-quality DNA via this method was re-extracted using the Maxwell RSC instrument and the recommended Blood DNA kit (Promega), according to the manufacturer’s instructions. A DNA concentration

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