Maxwell RSC Instrument from Promega

Product Specs
ItemMaxwell RSC Instrument
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS4500
QuantityEA
Processing Time30 to 60 min (depending on sample type and method)
CapacityUp to 16
Dimensions(WxDxH) 330.2 x 345.2 x 299.7 mm (13 x 13.6 x 11.8 in)
UV LampAvg. lifetime approximately 6,000 hrs
Molecular Weight~85kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(621) Comparative analysis of post-transplant lymphoproliferative disorders after solid organ and hematopoietic stem cell transplantation reveals differences in the tumor microenvironmentVirchows ArchivJune 1, 2021Mathis Overkamp, Massimo Granai, Irina Bonzheim, Julia Steinhilber, Jens Schittenhelm, Wolfgang Bethge, Leticia Quintanilla-Martinez, Falko Fend, Birgit Federmannwas extracted from macrodissected 5-μm paraffin sections using the Maxwell® RSC DNA FFPE Kit and the Maxwell® RSC Instrument (Promega, Madison, WI, USA) according to the manufacturer’s instructions. The PCR for IGH gene rearrangements

(622) Detection of PIK3CA Gene Mutation in Head and Neck Squamous Cell Carcinoma Using Droplet Digital PCR and RT-qPCRBiomoleculesMay 31, 2021Edyta M. Borkowska, Magda Barańska, Magdalena Kowalczyk, Wioletta PietruszewskaMaxwell RSC DNA FFPE Kit (Promega Corporations, Madison, WI, USA, cat No AS1450) and automatic isolation on AS4500 device (Promega Corporations, Madison, WI, USA). The purity of extracted genomic DNA was checked by measuring

(623) Classification of molecular subtypes of high-grade serous ovarian cancer by MALDI-ImagingJournal of Clinical OncologyMay 20, 2021Wanja Nikolai Kassuhn et al.Classification by NanoString Technology Total RNA extraction was performed on FFPE tissue sections using the Maxwell RSC instrument by Promega (Promega GmbH, Walldorf, Germany) following the manufacturer’s instructions. Two 5 µm thick

(624) Digital Droplet PCR for SARS-CoV-2 Resolves Borderline CasesAmerican Journal of Clinical PathologyMay 18, 2021Jing Xu, Timothy Kirtek, Yan Xu, Hui Zheng, Huiyu Yao, Emily Ostman, Dwight Oliver, James S Malter, Jeffrey R Gagan, Jeffrey A SoRellenucleic acids from pooled or individual residual NP collections and controls were obtained using a Maxwell RSC instrument (Promega), with an offboard lysis protocol and the following volume parameters: 400 µL input, 500 µL

(625) Molecular Detection and Phylogeny of Tick-Borne Pathogens in Ticks Collected from Dogs in the Republic of KoreaPathogensMay 17, 2021A-Tai Truong, Jinhyeong Noh, Yeojin Park, Hyun-Ji Seo, Keun-Ho Kim, Subin Min, Jiyeon Lim, Mi-Sun Yoo, Heung-Chul Kim, Terry A. Klein, et al.Precellys 24 tissue homogenizer (Bertin Instruments, Montigny-le-Bretonux, France). The tick homogenate was placed in a Maxwell RSC instrument (Promega, Madison, WI, USA) according to the manufacturer’s instructions. The purification of the total

(626) Characterization of Plasma Cell-Free DNA Integrity Using Droplet-Based Digital PCR: Toward the Development of Circulating Tumor DNA-Dedicated AssaysFrontiers in OncologyMay 6, 2021Geoffroy Poulet et al.with the use of the Maxwell RSC ccfDNA Plasma kit (Promega; called Ext.kit 1 in the manuscript) using Maxwell® RSC Instrument. In the PLACOL study and corresponding control group, plasma from healthy individuals or mCRC patients

(627) Genetic Causes of Cardiomyopathy in Children: First Results From the Pediatric Cardiomyopathy Genes StudyJournal of the American Heart AssociationMay 4, 2021Stephanie M. Ware, James D. Wilkinson, Muhammad Tariq, Jeffrey A. Schubert, Arthi Sridhar, Steven D. Colan, Ling Shi, Charles E. Canter, Daphne T. Hsu, Steven A. Webber, et al.were collected at each site and shipped to the Genetic Coordinating Center. DNA was extracted on a Maxwell RSC instrument (Promega, Madison, WI) according to the manufacturer’s protocol. Exome sequencing was performed using sequence

(628) Chromosome 2q gain and epigenetic silencing of GATA3 in microglandular adenosis of the breastThe Journal of Pathology: Clinical ResearchMay 1, 2021Martin Radner, Jana Lisa van Luttikhuizen, Stephan Bartels, Janin Bublitz, Isabel Grote, Luisa Rieger, Henriette Christgen, Helge Stark, Christopher Werlein, Marcel Lafos, et al.and RNA were extracted with the Maxwell RSC DNA and RNA FFPE kits (Promega, Madison, WI,USA) using a Maxwell RSC instrument (Promega) according to the manufacturer's recommendations. DNA was quantified using a Qubit 2.0 fluorometer

(629) L2HGDH Missense Variant in a Cat with L-2-Hydroxyglutaric AciduriaGenes (Basel)May 1, 2021Matthias Christen, Nils Janzen, Anne Fraser, Adrian C. Sewell, Vidhya Jagannathan, Julien Guevar, Tosso Leeb, Daniel Sanchez-MasianDNA Extraction Genomic DNA was isolated from EDTA blood with the Maxwell RSC Whole Blood Kit using a Maxwell RSC instrument (Promega, Dübendorf, Switzerland). 2.7. Whole-Genome Sequencing An Illumina TruSeq PCR-free DNA library

(630) Cranial Mandibular Fibrosis Syndrome in Adult Farmed Rainbow Trout Oncorhynchus mykissPathogensApril 30, 2021Irene Cano, John Worswick, Brian Mulhearn, Matt Green, Stephen W. Feist, Morag Clintonsupernatant of RTG-2 cells showing CPEs using a Maxwell® RSC viral total nucleic acid purification kit in a Maxwell® RSC instrument (Promega, Southampton, UK) following the manufacturer’s instructions. Reverse transcription (RT) was

(631) MicroRNA Expression in Extracellular Vesicles from Nasal Lavage Fluid in Chronic RhinosinusitisBiomedicinesApril 26, 2021Seungbin Cha, Eun-Hye Seo, Seung Hyun Lee, Kyung Soo Kim, Chung-Sik Oh, Jong-Seok Moon, Jin Kook Kimusing the Promega Maxwell® RSC miRNA Tissue Kit (AS1460; Promega Corporation, Fitchburg, WI, USA) and Maxwell® RSC instrument (Promega Corporation, Madison, WI, USA) according to the manufacturer’s instructions. The concentration

(632) Isolation and Characterization of Phosphate Solubilizing Streptomyces sp. Endemic from Sugar Beet Fields of the Beni-Mellal Region in MoroccoMicroorganismsApril 24, 2021Yassine Aallam, Driss Dhiba, SanaÃ¢ Lemriss, Amal Souiri, Fatma Karray, Taoufik El Rasafi, Nezha SaÃ¯di, Abdelmajid Haddioui, SaÃ¢d El Kabbaj, Marie JoÃ«lle Virolle, et al.centrifugation (16,000× g for 10 min) and the mycelial pellet was used for automated extraction of DNA with the Maxwell® RSC Instrument (Promega, Madison, WI, USA) and the Maxwell® RSC PureFood GMO and Authentication Kit (Promega) according

(633) Several Fusion Genes Identified in a Spermatic Cord Leiomyoma With Rearrangements of Chromosome Arms 3p and 21qCancer Genomics - ProteomicsApril 18, 2021IOANNIS PANAGOPOULOS, LUDMILA GORUNOVA, KRISTIN ANDERSEN, INGVILD LOBMAIER, SVERRE HEIManalyzed cytogenetically as previously described (25). Genomic DNA was extracted from the tumor using the Maxwell RSC Instrument and Maxwell RSC Tissue DNA Kit (Promega, Madison, WI, USA), the concentration was measured using Quantus Fluorometer

(634) Reactive astrocytes in ALS display diminished intron retentionNucleic Acids ResearchApril 6, 2021Oliver J Ziff, Doaa M Taha, Hamish Crerar, Benjamin E Clarke, Anob M Chakrabarti, Gavin Kelly, Jacob Neeves, Giulia E Tyzack, Nicholas M Luscombe, Rickie Pataniisolation and RT-qPCR The Promega Maxwell RSC simplyRNA cells kit including DNase treatment, alongside the Maxwell RSC instrument, was used for RNA extractions. The Nanodrop was used to assess RNA concentration and the 260/280 ratio,

(635) Circulating HPV DNA in the Management of Oropharyngeal and Cervical Cancers: Current Knowledge and Future PerspectivesJournal of Clinical MedicineApril 6, 2021Eriseld Krasniqi et al.a refrigerated centrifuge, serum samples were stored at –80 ◦ C. DNA was extracted with an automated Maxwell RSC Instrument (Promega). To detect single copies of HPV16 or HPV18 DNA, a ddPCR method was developed using the sequences

(636) CovidArray: A Microarray-Based Assay with High Sensitivity for the Detection of Sars-Cov-2 in Nasopharyngeal SwabsSensors (Basel)April 3, 2021Francesco Damin, Silvia Galbiati, Stella Gagliardi, Cristina Cereda, Francesca Dragoni, Claudio Fenizia, Valeria Savasi, Laura Sola, Marcella Chiarithe five nasopharyngeal swabs preservation media (from L. Sacco COVID19-hub Hospital) employing the Maxwell® RSC Instrument (Promega, Fitchburg, WI, USA) while RNAs from 350 µL of the seven nasopharyngeal swabs UTM (IRCCS Mondino

(637) Gut microbiota composition reflects disease severity and dysfunctional immune responses in patients with COVID-19GutApril 1, 2021Yun Kit Yeoh, Tao Zuo, Grace Chung-Yan Lui, Fen Zhang, Qin Liu, Amy YL Li, Arthur CK Chung, Chun Pan Cheung, Eugene YK Tso, Kitty SC Fung, et al.0.1 g of homogenised faecal samples using the Maxwell RSC PureFood GMO and Authentication Kit and a Maxwell RSC Instrument nucleic acid extraction platform (Promega, Wisconsin, USA) according to manufacturer’s instructions.

(638) Human Pumilio proteins directly bind the CCR4-NOT deadenylase complex to regulate the transcriptomeRNAApril 1, 2021Isioma I.I. Enwerem, Nathan D. Elrod, Chung-Te Chang, Ai Lin, Ping Ji, Jennifer A. Bohn, Yevgen Levdansky, Eric J. Wagner, Eugene Valkov, Aaron C. Goldstrohm× 106 HCT116 cells harvested 48 h following reporter transfection using the SimplyRNA cells kit and Maxwell RSC instrument (Promega) following the manufacturer's protocol. Twice the amount of DNase was added for the on-bead

(639) Somatic Mutation Profiling in the Liquid Biopsy and Clinical Analysis of Hereditary and Familial Pancreatic Cancer Cases Reveals KRAS Negativity and a Longer Overall SurvivalCancers (Basel)March 31, 2021Julie Earl, Emma Barreto, MarÃa E. Castillo, Raquel Fuentes, Mercedes RodrÃguez-Garrote, Reyes Ferreiro, Pablo Reguera, Gloria MuÃ±oz, David Garcia-Seisdedos, Jorge VillalÃ³n LÃ³pez, et al.circulating nucleic acid kit (Qiagen, Venlo, Netherlands). DNA was extracted from 1 mL of plasma using the Maxwell® RSC Instrument (Promega, Madison, WI, USA) and eluted in a final volume of 50 µL from 136 patients diagnosed with PDAC

(640) Classification of Molecular Subtypes of High-Grade Serous Ovarian Cancer by MALDI-ImagingCancers (Basel)March 25, 2021Wanja Kassuhn et al.Classification by NanoString Technology Total RNA extraction was performed on FFPE tissue sections using the Maxwell RSC instrument by Promega (Promega GmbH, Walldorf, Germany) following the manufacturer’s instructions. Two 5 µm thick

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