Maxwell RSC Instrument from Promega

Product Specs
ItemMaxwell RSC Instrument
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS4500
QuantityEA
Processing Time30 to 60 min (depending on sample type and method)
CapacityUp to 16
Dimensions(WxDxH) 330.2 x 345.2 x 299.7 mm (13 x 13.6 x 11.8 in)
UV LampAvg. lifetime approximately 6,000 hrs
Molecular Weight~85kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(341) Human motor neurons derived from induced pluripotent stem cells are susceptible to SARS-CoV-2 infectionFront Cell NeurosciDecember 5, 2023Gioia Cappelletti, Claudia Colombrita, Fiona Limanaqi, Sabrina Invernizzi, Micaela Garziano, Claudia Vanetti, Claudia Moscheni, Serena Santangelo, Silvia Zecchini, Daria Trabattoni, et al.assessment For SARS-CoV-2 replication assessment, RNA was extracted from iPSC-MN supernatants using the Maxwell® RSC Instrument with Maxwell® RSC Viral Total Nucleic Acid Purification Kit (Promega, Fitchburg, WI, USA). Viral RNA

(342) Semaphorin 3C promotes de novo steroidogenesis in prostate cancer cellsEndocrine-related cancerDecember 1, 2023Yenki P, Bhasin S, Liu L, Nabavi N, Cheng CW, Tam KJ, Peacock JW, Adomat HH, Tombe T, Fazli L, et al.RSC simply RNA Tissue Kit (Promega, Cat. No. AS1340) using Maxwell RSC Instrument (Promega, Cat. No. AS4500). Following quality control by TapeStation system, strand-specific RNA library was prepared using TruSeq

(343) Utilizing Molecular Epidemiology and Citizen Science for the Surveillance of Lagoviruses in AustraliaVirusesNovember 29, 2023Nias Y. G. Peng, Robyn N. Hall, Nina Huang, Peter West, Tarnya E. Cox, Jackie E. Mahar, Hugh Mason, Susan Campbell, Tiffany O’Connor, Andrew J. Read, et al.the community submissions. RNA from tissue samples (liver and bone marrow) was extracted using the Maxwell® RSC instrument (Promega, Madison, WI, USA) in combination with the Maxwell® RSC SimplyRNA Tissue Kit (Promega) or the

(344) Proteomic analyses identify HK1 and ATP5A to be overexpressed in distant metastases of lung adenocarcinomas compared to matched primary tumorsScientific reportsNovember 28, 2023Pasternack H, Polzer M, Gemoll T, Kümpers C, Sauer T, Lazar-Karsten P, Hinrichs S, Bohnet S, Perner S, Dressler FF, et al.acid extractions. Isolation of genomic DNA was performed using the Maxwell RSC DNA FFPE Kit and the Maxwell RSC instrument (Promega, Fitchburg WI, U.S.A.). DNA samples were quantified using the Qubit fluorimeter (TermoFisher,

(345) Wheat stripe rust resistance locus YR63 is a hot spot for evolution of defence genes – a pangenome discoveryBMC Plant BiologyNovember 27, 2023Amy Mackenzie, Michael Norman, Mesfin Gessese, Chunhong Chen, Chris Sørensen, Mogens Hovmøller, Lina Ma, Kerrie Forrest, Lee Hickey, Harbans Bariana, et al.was extracted using the Maxwell® RSC Plant RNA Kit (Promega) using the manufacturers protocol on the Maxwell® RSC instrument. RNA samples were sent to Novogene for paired end read sequencing. Quality of the raw RNA reads was

(346) Genetic Variants in Matrix Metalloproteinases MMP3 (rs3025058) and MMP9 (rs3918242) Associated with Colonic DiverticulosisMedicina (Kaunas)November 17, 2023Piotr Nehring, Grażyna Gromadzka, Miłosz Jastrzębski, Adam Przybyłkowski−80 °C. Genetic material was isolated from whole blood samples collected in EDTA tubes employing the Maxwell® RSC Instrument and Maxwell® 16 Blood DNA Purification Kit (Promega Corporation, Madison, WI, USA). The genotyping was

(347) Comparative Study of Intestinal Microbiome in Patients with Ulcerative Colitis and Healthy Controls in KoreaMicroorganismsNovember 11, 2023Kyung-Hyo Do, Seung-Hyun Ko, Ki Bae Kim, Kwangwon Seo, Wan-Kyu Lee°C. Bacterial DNA was extracted from 200 mg of fecal sample collected from each individual using a Maxwell RSC Instrument (Promega, Madison, WI, USA) and a Maxwell RSC Fecal Microbiome DNA Kit AS1700 (Promega), according to

(348) Standardization of Molecular MRD Levels in AML Using an Integral Vector Bearing ABL and the Mutation of InterestCancers (Basel)November 10, 2023Boaz Nachmias, Svetlana Krichevsky, Moshe E. Gatt, Noa Gross Even-Zohar, Adir Shaulov, Arnon Haran, Shlomzion Aumann, Vladimir VainsteinMaxwell® RSC simply RNA Blood Kit (Promega, Madison, WI, USA) were used for DNA and RNA extraction with a Maxwell® RSC Instrument (Promega, Madison, WI, USA). cDNA was prepared from 2-µg RNA using the High-Capacity cDNA Reverse Transcription

(349) A pig model of chronic hepatitis E displaying persistent viremia and a downregulation of innate immune responses in the liverHepatol CommunNovember 8, 2023Nancy León-Janampa, Ignacio Caballero-Posadas, Céline Barc, François Darrouzain, Alain Moreau, Thibault Guinoiseau, Philippe Gatault, Isabelle Fleurot, Mickaël Riou, Anne Pinard, et al.solution. RNA was extracted from the tissue lysates with the Maxwell RSC simplyRNA Tissue Kit and a Maxwell RSC instrument (Promega). HEV RNA was quantified by ORF3 RT-qPCR (limit of detection: 2 log IU/mL), as described.35

(350) A pig model of chronic hepatitis E displaying persistent viremia and a downregulation of innate immune responses in the liverHepatology CommunicationsNovember 8, 2023Nancy León-Janampa, Ignacio Caballero-Posadas, Céline Barc, François Darrouzain, Alain Moreau, Thibault Guinoiseau, Philippe Gatault, Isabelle Fleurot, Mickaël Riou, Anne Pinard, et al.solution. RNA was extracted from the tissue lysates with the Maxwell RSC simplyRNA Tissue Kit and a Maxwell RSC instrument (Promega). HEV RNA was quantified by ORF3 RT-qPCR (limit of detection: 2 log IU/mL), as described.35

(351) Naturally occurring canine laminopathy leading to a dilated and fibrosing cardiomyopathy in the Nova Scotia Duck Tolling RetrieverScientific ReportsNovember 4, 2023Danika L. Bannasch, Danielle T. Oertle, Julia Vo, Kevin L. Batcher, Joshua A. Stern, Joanna L. Kaplan, Ronald H. L. Li, Indiana E. Madden, Matthias Christen, Tosso Leeb, et al.samples according to standard methods using the Maxwell RSX Whole Blood DNA kit in combination with the Maxwell RSC instrument (Promega, Dübendorf, Switzerland). Genome-wide association study The Illumina Canine HD BeadChip was

(352) Food Administration and Not Genetic Variants Causes Pharmacokinetic Variability of Tadalafil and FinasterideJournal of Personalized MedicineOctober 31, 2023Gonzalo Villapalos-García, Pablo Zubiaur, Cristina Marián-Revilla, Paula Soria-Chacartegui, Marcos Navares-Gómez, Gina Mejía-Abril, Andrea Rodríguez-Lopez, Eva González-Iglesias, Samuel Martín-Vílchez, Manuel Román, et al.performed using either a MagNa Pure automatic DNA extractor (Roche Applied Science, Penzberg, Germany) or a Maxwell® RSC instrument (Promega, Madison, WI, USA). DNA concentration was measured with a Qubit 3.0 Fluorometer (ThermoFisher,

(353) Spermidine Ameliorates Colitis via Induction of Anti-Inflammatory Macrophages and Prevention of Intestinal DysbiosisJournal of Crohn's and ColitisOctober 20, 2023Anna Niechcial, Marlene Schwarzfischer, Marcin Wawrzyniak, Kirstin Atrott, Andrea Laimbacher, Yasser Morsy, Egle Katkeviciute, Janine Häfliger, Patrick Westermann, Cezmi A Akdis, et al.[PBS], and RNA isolation was performed using the Maxwell RSC simplyRNA Tissue Kit [Promega] and the Maxwell RSC Instrument [Promega] according to the manufacturer’s instructions. RNA concentration was determined by measuring

(354) Genetic signature detected in T cell receptors from patients with severe COVID-19iScienceOctober 20, 2023Manuel Corpas, Carmen de Mendoza, Víctor Moreno-Torres, Ilduara Pintos, Pedro Seoane, James R. Perkins, Juan A.G. Ranea, Segun Fatumo, Tamas Korcsmaros, José Manuel Martín-Villa, et al.isolated from buffy coat frozen samples with the Maxwell RSC buffy coat DNA kit (Promega) using the Maxwell RSC Instrument (Promega), following manufacturer recommendations. After isolation, the purity of genomic DNA was analyzed

(355) Epithelial ovarian cancer is infiltrated by activated effector T cells co-expressing CD39, PD-1, TIM-3, CD137 and interacting with cancer cells and myeloid cellsFrontiers in ImmunologyOctober 4, 2023Elena Tassi, Alice Bergamini, Jessica Wignall, Miriam Sant’Angelo, Emanuela Brunetto, Chiara Balestrieri, Miriam Redegalli, Alessia Potenza, Danilo Abbati, Francesco Manfredi, et al.calculated. Gene expression analysis RNA was extracted from FFPE samples by using Maxwell RSC RNA FFPE Kit and Maxwell RSC Instrument (Promega), according to the manufacturer’s instructions. Elution was performed in 50 μl and RNA was

(356) The Virucidal Effect of the Chlorination of Water at the Initial Phase of Disinfection May Be Underestimated If Contact Time Calculations Are UsedPathogensOctober 3, 2023Fredy Saguti, Inger Kjellberg, Marianela Patzi Churqui, Hao Wang, Timur Tunovic, Jakob Ottoson, Olof Bergstedt, Helene Norder, Kristina Nyströmeach three viruses from both control and treated samples with disinfectants in Maxwell RSC instrument AS4500 (Promega, Madison, WI, USA) using Maxwell RSC Viral Total Nucleic Acid Purification kit (Promega, WI,

(357) UV-radiation and MC1R germline mutations are risk factors for the development of conventional and spitzoid melanomas in children and adolescentseBioMedicineOctober 1, 2023Alexandra Liebmann, Jakob Admard, Sorin Armeanu-Ebinger, Hannah Wild, Michael Abele, Axel Gschwind, Olga Seibel-Kelemen, Christian Seitz, Irina Bonzheim, Olaf Riess, et al.tumour and normal tissue paraffin sections using the Maxwell® RSC DNA FFPE Plus Kit (AS1720) and the Maxwell® RSC Instrument (Promega, Madison, WI, USA) according to the manufacturer’s instructions. Three samples had to be excluded

(358) Genetic variants in melanogenesis proteins TYRP1 and TYR are associated with the golden rhesus macaque phenotypeG3: Genes, Genomes, GeneticsSeptember 30, 2023Samuel M Peterson, Marina M Watowich, Lauren M Renner, Samantha Martin, Emma Offenberg, Amanda Lea, Michael J Montague, James P Higham, Noah Snyder-Mackler, Martha Neuringer, et al.accessed through the ONPRC Genetic Core DNA Bank or extracted from archived tissue samples using a Maxwell RSC Instrument and following the manufacturer's protocol (Promega, Inc.). Genotyping-by-sequencing (GBS) libraries

(359) CD8+ cells and small viral reservoirs facilitate post-ART control of SIV replication in M3+ Mauritian cynomolgus macaques initiated on ART two weeks post-infectionPLOS PathogensSeptember 25, 2023Olivia E. Harwood, Lea M. Matschke, Ryan V. Moriarty, Alexis J. Balgeman, Abigail J. Weaver, Amy L. Ellis-Connell, Andrea M. Weiler, Lee C. Winchester, Courtney V. Fletcher, Thomas C. Friedrich, et al.lymph node (LN) mononuclear cells (LNMC) isolated from LN biopsies using the SimplyRNA Tissue kit for the Maxwell RSC instrument (Promega, Madison, WI). RNA was then tested for both SIV gag and a cellular gene, β-actin, by RT-qPCR.

(360) Next-generation sequencing improves precision medicine in hearing lossFrontiers in GeneticsSeptember 22, 2023T. Imizcoz, C. Prieto-Matos, R. Manrique-Huarte, D. Calavia, A. Huarte, P. C. Pruneda, G. R. Ordoñez, E. Cañada-Higueras, A. Patiño-García, G. Alkorta-Aranburu, et al.bp of flanking intronic regions. Briefly, DNA was isolated from peripheral blood or saliva using the Maxwell RSC Instrument (Promega) and characterized by Qubit (ThermoFisher). Target-region capture using Agilent SureSelect

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