Maxwell RSC Instrument from Promega

Product Specs
ItemMaxwell RSC Instrument
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS4500
QuantityEA
Processing Time30 to 60 min (depending on sample type and method)
CapacityUp to 16
Dimensions(WxDxH) 330.2 x 345.2 x 299.7 mm (13 x 13.6 x 11.8 in)
UV LampAvg. lifetime approximately 6,000 hrs
Molecular Weight~85kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(341) Precision metagenomics sequencing for food safety: hybrid assembly of Shiga toxin-producing Escherichia coli in enriched agricultural waterFrontiers in MicrobiologyAugust 31, 2023Meghan Maguire, Padmini Ramachandran, Sandra Tallent, Mark K. Mammel, Eric W. Brown, Marc W. Allard, Steven M. Musser, Narjol González-Escalonacontaminated sample, Water and Water + Ecoli1–5, using the Maxwell RSC Cultured Cells DNA kit with a Maxwell RSC Instrument (Promega Corporation, Madison, WI) according to the manufacturer's instructions for Gram-negative bacteria

(342) The relation of ApoE and COMT gene–gene interactions to cognitive and motor function in community-dwelling older adults: a pilot studyFrontiers in Aging NeuroscienceAugust 31, 2023Kendra L. Pizzonia, Julie A. Suhr, Leatha A. Clark, Brian C. ClarkNucleic Acid Extraction instrument (Promega Corporation, 2,800 Woods Hollow Road Madison, WI; Cat. #AS4500) and Maxwell® RSC Buccal Swab DNA Kit (Cat. #AS1640) as we have previously described (Moskowitz et al.,

(343) Identification of an ADAMTS2 frameshift variant in a cat family with Ehlers–Danlos syndromeG3: Genes, Genomes, GeneticsAugust 30, 2023Rebecca Simon, Sarah Kiener, Nina Thom, Laura Schäfer, Janina Müller, Elfi K Schlohsarczyk, Ulrich Gärtner, Christiane Herden, Tosso Leeb, Gesine LühkenDNA was isolated from muscle tissue of the deceased kitten using a Maxwell RSC Tissue DNA Kit and a Maxwell RSC instrument (Promega, Dübendorf, Switzerland). For genotyping, DNA extraction from buccal swaps (sterile transport

(344) Investigations into SARS-CoV-2 and other coronaviruses on mink farms in France late in the first year of the COVID-19 pandemicPLoS OneAugust 25, 2023Marine Wasniewski, Franck Boué, Céline Richomme, Etienne Simon-Lorière, Sylvie Van der Werf, Flora Donati, Vincent Enouf, Yannick Blanchard, Véronique Beven, Estelle Leperchois, et al.of lysis buffer. RNA was extracted with the Maxwell RSC PureFood GMO and Authentication kit using a Maxwell RSC instrument (Promega France, Charbonnières les Bains, France), according to the manufacturer’s instructions. RNA

(345) Real-World Data on Combined EGFR-TKI and Crizotinib Treatment for Acquired and De Novo MET Amplification in Patients with Metastatic EGFR-Mutated NSCLCInternational Journal of Molecular SciencesAugust 23, 2023Edyta M. Urbanska, Morten Grauslund, Peter R. Koffeldt, Sarah L. B. Truelsen, Johan O. Löfgren, Junia C. Costa, Linea C. Melchior, Jens B. Sørensen, Eric Santoni-Rugiumanufacturer’s instructions. RNA was purified from diagnostic biopsies and tumor rebiopsies utilizing the Maxwell RSC instrument (Promega, Madison, WI, USA) with a Maxwell RSC RNA FFPE kit (Promega) and quantified with the Qubit™

(346) Manipulation of components of the renin angiotensin system in renal proximal tubules fails to alter atherosclerosis in hypercholesterolemic miceFrontiers in Cardiovascular MedicineAugust 16, 2023Masayoshi Kukida, Naofumi Amioka, Dien Ye, Hui Chen, Jessica J. Moorleghen, Ching-Ling Liang, Deborah A. Howatt, Yuriko Katsumata, Motoko Yanagita, Hisashi Sawada, et al.of the renal cortex was extracted using a commercial kit (Cat # AS1340, Promega) configured with a Maxwell RSC Instrument (Promega). To quantify mRNA abundance, total RNA was reversely transcribed with an iScript™ cDNA Synthesis kit

(347) Trade-offs between immunity and competitive ability in fighting ant malesBMC Ecol EvolAugust 7, 2023Sina Metzler, Jessica Kirchner, Anna V Grasse, Sylvia Cremertranscribed to cDNA before performing gene expression analysis using ddPCR. Total RNA was extracted using the Maxwell® RSC instrument together with the Maxwell® simplyRNA Tissue Kit (Promega) according to the manufacturer’s instructions

(348) Concordance in detection of microsatellite instability by PCR and NGS in routinely processed tumor specimens of several cancer typesCancer MedicineAugust 1, 2023Stephan Bartels, Isabel Grote, Madeleine Wagner, Jannik Boog, Elisa Schipper, Tanja Reineke‐Plaass, Hans Kreipe, Ulrich Lehmannfrom serial unstained sections. DNA isolation was performed with the Maxwell© RSC DNA FFPE kit on a Maxwell© RSC instrument (Promega, Madison). Measurement of the DNA concentration was performed with dsDNA high sensitivity kit

(349) Comparative Genetic Characterization of CTX-M-Producing Escherichia coli Isolated from Humans and Pigs with Diarrhea in Korea Using Next-Generation SequencingMicroorganismsJuly 28, 2023Kwang-Won Seo, Kyung-Hyo Do, Wan-Kyu LeeExtraction and Quantification Genomic DNA from 24 CTX-M-producing E. coli was extracted using the Maxwell RSC Instrument (Promega, Madison, WI, USA) and Maxwell® RSC Cultured Cells DNA Kit AS1620 (Promega), according to the

(350) Genomic and functional portrait of multidrug-resistant, hydrogen sulfide (H2S)-producing variants of Escherichia coliFrontiers in MicrobiologyJuly 27, 2023Razib Mazumder, Arif Hussain, Mohammad Mustafizur Rahman, Jody E. Phelan, Susana Campino, Ahmed Abdullah, Taane G. Clark, Dinesh Mondalclasses (Magiorakos et al., 2012). Whole-genome sequencing Total bacterial DNA was extracted using the Maxwell RSC Instrument and Culture Cell DNA extraction Kit (Promega) for gram-negative bacteria with an additional RNaseA treatment

(351) RALGAPA1 Deletion in Belgian Shepherd Dogs with Cerebellar AtaxiaGenes (Basel)July 25, 2023Matthias Christen, Isabel Zdora, Michael Leschnik, Vidhya Jagannathan, Christina Puff, Enrice Hünerfauth, Holger A. Volk, Wolfgang Baumgärtner, Tessa C. Koch, Wencke Schäfer, et al.Extraction We extracted genomic DNA from EDTA blood samples with a Maxwell RSC whole blood DNA kit using a Maxwell RSC instrument (Promega, Madison, WI, USA). A Maxwell RSC DNA FFPE kit was used for DNA isolation from FFPE tissue

(352) A Comparative Analysis of NOX4 Protein Expression in Malignant and Non-Malignant Thyroid TumorsCurrent Issues in Molecular BiologyJuly 13, 2023Salma Fenniche, Mohamed Oukabli, Yassire Oubaddou, Hafsa Chahdi, Amal Damiri, Abir Alghuzlan, Abdelilah Laraqui, Nadia Dakka, Youssef Bakri, Corinne Dupuy, et al.DNA was extracted using the ‘Maxwell® RSC DNA FFPE Kit’ from Promega/‘Maxwell® RSC Instruments (Cat.# AS4500, AS8500)’ according to the manufacturer’s protocols (ASB1450). The extracted genomic DNA was sequenced

(353) A low-coverage 3′ RNA-seq to detect homeolog expression in polyploid wheatNAR Genomics and BioinformaticsJuly 12, 2023Jianqiang Sun, Moeko Okada, Toshiaki Tameshige, Rie Shimizu-Inatsugi, Reiko Akiyama, Atsushi J Nagano, Jun Sese, Kentaro K Shimizuand H2SO4 to adjust pH 5.2). The total RNA was extracted using the Maxwell® RSC Plant RNA Kit with Maxwell® RSC Instrument (Promega Corp., Madison, WI) according to the manufacturer’s instructions. For each of the six CS RNA

(354) Expression and possible functions of a horizontally transferred glycosyl hydrolase gene, GH6-1, in Ciona embryogenesisEvoDevoJuly 11, 2023Kun-Lung Li, Keisuke Nakashima, Kanako Hisata, Noriyuki Satohefficiency. Genomic DNA of 15–30 embryos (12–16 hpf) was extracted with a Maxwell RSC Blood DNA Kit on a Maxwell RSC Instrument (Promega). Extracted genomic DNA was amplified with primers targeting a part of the GH6-1 gene; Forward:

(355) Secondary leiomyosarcoma of the nasal cavity in a treated patient with possible hereditary retinoblastoma with germline reciprocal translocation of RB1 and DMXL1 and somatic TP53 mutation: A case reportMolecular and Clinical OncologyJuly 10, 2023Toshinari Yagi, Harumi Nakamura, Yoji Kukita, Toru Wakamatsu, Hironari Tamiya, Shou Nakai, Makiyo Watanabe, Shigeki Kakunaga, Haruna Takami, Rie Suzuki, et al.formalin-fixed, paraffin-embedded (FFPE) tissues RNAs were extracted from slices of FFPE tissue using Maxwell RSC Instrument and Maxwell RCS RNA FFPE Kit (Promega) and their concentrations were measured with the QuantiFluor RNA System

(356) Evolution of a globally unique SARS-CoV-2 Spike E484T monoclonal antibody escape mutation in a persistently infected, immunocompromised individualVirus EvolutionJuly 7, 2023Peter J Halfmann, Nicholas R Minor, Luis A Haddock III, Robert Maddox, Gage K Moreno, Katarina M Braun, David A Baker, Kasen K Riemersa, Ankur Prasad, Kirsten J Alman, et al.isolated viral RNA from an additional nasopharyngeal swab sample using the Total Nucleic Acid kit for the Maxwell RSC instrument (Promega, Madison, WI). We used the N1 qRT-PCR assay developed by the United States Centers for Disease

(357) Investigations on SARS-CoV-2 and other coronaviruses in mink farms in France at the end of the first year of COVID-19 pandemicbioRxivJuly 7, 2023Marine Wasniewski, Franck Boué, Céline Richomme, Etienne Simon-Lorière, Sylvie Van der Werf, Flora Donati, Vincent Enouf, Yannick Blanchard, Véronique Beven, Estelle Leperchois, et al.Lysis buffer. RNA extraction was performed with the Maxwell RSC PureFood GMO and Authentication kit using a Maxwell RSC instrument (Promega France, Charbonnières les Bains, France), according to the manufacturer’s instructions. RNA

(358) Novel potentially pathogenic variants detected in genes causing intellectual disability and epilepsy in Polish familiesneurogeneticsJuly 5, 2023S. Skoczylas, P. Jakiel, T. Płoszaj, K. Gadzalska, M. Borowiec, A. Pastorczak, H. Moczulska, M. Malarska, A. Eckersdorf-Mastalerz, E. Budzyńska, et al.list of genes is described in Supplementary Table 1. DNA was extracted from peripheral blood using Maxwell® RSC Instrument (Maxwell® RSC Blood DNA Kit, Promega, Madison, USA). The library was prepared using the Agilent SureSelectQXT

(359) A Two-Step Diagnostic Approach for NTRK Gene Fusion Detection in Biliary Tract and Pancreatic AdenocarcinomasThe OncologistJuly 5, 2023Anne Demols, Laureen Rocq, Luis Perez-Casanova, Manon Charry, Nancy De Nève, Ali Ramadhan, Claude Van Campenhout, Sarah De Clercq, Calliope Maris, Jean Closset, et al.Quantification Nucleic acids were extracted from FFPE tumor samples, after macrodissection of the tumor area, using the Maxwell RSC Instrument (Promega Corporation, Madison, WI, USA) according to the manufacturer’s instructions and as already

(360) Wastewater-Based Epidemiology to Describe the Evolution of SARS-CoV-2 in the South-East of Spain, and Application of Phylogenetic Analysis and a Machine Learning ApproachVirusesJuly 3, 2023Jose A. Férez, Enric Cuevas-Ferrando, María Ayala-San Nicolás, Pedro J. Simón Andreu, Román López, Pilar Truchado, Gloria Sánchez, Ana Allendeto the loading cartridge, and 300 μL of lysis buffer was added. The cartridge was then loaded in the Maxwell® RSC Instrument (Promega, Madison, WI, USA) using the “Maxwell RSC Viral total Nucleic Acid” running program for the

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