Maxwell RSC Instrument from Promega

Product Specs
ItemMaxwell RSC Instrument
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS4500
QuantityEA
Processing Time30 to 60 min (depending on sample type and method)
CapacityUp to 16
Dimensions(WxDxH) 330.2 x 345.2 x 299.7 mm (13 x 13.6 x 11.8 in)
UV LampAvg. lifetime approximately 6,000 hrs
Molecular Weight~85kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(361) Immunologic constant of rejection as a predictive biomarker of immune checkpoint inhibitors efficacy in non-small cell lung cancerJournal of Translational MedicineSeptember 19, 2023Alice Mogenet, Pascal Finetti, Emilie Denicolai, Laurent Greillier, Pascaline Boudou-Rouquette, François Goldwasser, Gwenael Lumet, Michele Ceccarelli, Daniel Birnbaum, Davide Bedognetti, et al.informed consent for the use of archived material for research purpose. Tumor RNA was extracted using “Maxwell® RSC Instrument” (Promega) shortly after microtome dissection to prevent nucleic acid degradation. After extraction,

(362) Lung microenvironments harbor Mycobacterium tuberculosis phenotypes with distinct treatment responsesAntimicrobial Agents and ChemotherapySeptember 19, 2023Nicholas D. Walter, Jackie P. Ernest, Christian Dide-Agossou, Allison A. Bauman, Michelle E. Ramey, Karen Rossmassler, Lisa M. Massoudi, Samantha Pauly, Reem Al Mubarak, Martin I. Voskuil, et al.min incubation at 37°C after each DNase addition. RNA was further purified and DNA was digested on a Maxwell RSC instrument (Promega) with the Maxwell RSC simplyRNA tissue kit. Purification was performed following the manufacturer’s

(363) Wastewater surveillance of Mpox virus in BaltimoreScience of The Total EnvironmentSeptember 15, 2023Samendra P. Sherchan, Tamunobelema Solomon, Oladele Idris, Daniel Nwaubani, Ocean Thakaliincrease within a month,"Wannigama et al., 2023" Spain,Grab,Aluminum adsorption-precipitation method,Maxwell® RSC Instrument (Promega) using the Maxwell RSC Pure Food GMO and authentication kit (Promega),CDC G2R_G and G2R_WA

(364) Evaluation of commercial kits for isolation and bisulfite conversion of circulating cell-free tumor DNA from bloodClinical EpigeneticsSeptember 14, 2023Stine H. Kresse, Sara Brandt-Winge, Heidi Pharo, Bjørnar T. B. Flatin, Marine Jeanmougin, Hege Marie Vedeld, Guro E. LindcfDNA isolation was performed according to the manufacturer’s instructions. For the Maxwell kit, the Maxwell RSC Instrument (Promega) was used for automated isolation according to the manufacturer’s instructions. For the CNA

(365) Targeting microRNA-145-mediated progressive phenotypes of early bladder cancer in a molecularly defined in vivo modelMolecular Therapy - Nucleic AcidsSeptember 12, 2023Kazuki Heishima, Nobuhiko Sugito, Chikara Abe, Akihiro Hirata, Hiroki Sakai, Yukihiro AkaoMaxwell RSC simplyRNA Cells Kit (Promega, Madison, WI, AS1390) and a Maxwell RSC instrument (Promega, AS4500). BC cells (253 JB-V) were transfected and incubated for 48 h with miRNAs (miR-145-5p, 10 nM; control

(366) Comparison of Two Variant Analysis Programs for Next-Generation Sequencing Data of Whole Mitochondrial GenomeJournal of Korean Medical ScienceSeptember 11, 2023Seung Eun Lee, Ga Eun Kim, Hajin Kim, Doo Hyun Chung, Soong Deok Lee, Moon-Young Kimsample, DNA was extracted using Maxwell RSC DNA FFPE kit (Promega Corporation, Madison, WI, USA) on Maxwell RSC instrument (Promega Corporation). It was quantified with Invitrogen Qubit Flex Fluorometer (Thermo Fisher Scientific),

(367) Implementation of pharmacogenetic testing in oncology: DPYD-guided dosing to prevent fluoropyrimidine toxicity in British ColumbiaFrontiers in PharmacologySeptember 8, 2023Angela Wu, Helen Anderson, Curtis Hughesman, Sean Young, Caroline Lohrisch, Colin J. D. Ross, Bruce C. Carleton(gDNA) was extracted from 150 µL of isolated buffy coat using the Maxwell RSC Buffy Coat DNA Kit and Maxwell RSC instrument (Promega, Wisconsin, United States). DPYD testing was performed by quantitative PCR (qPCR) on a QuantStudio

(368) Effects of a novel, non-invasive pre-hatch application of probiotic for broilers on development of cecum microbiota and production performanceAnimal MicrobiomeSeptember 5, 2023Kasper Rømer Villumsen, Dorthe Sandvang, Gisle Vestergård, Mia Son Räfle Olsen, Johanne Juul, Morten Dencker, Johannes Kudsk, Louise Ladefoged Poulsenbefore the supernatant was transferred to a new tube and used as DNA template. II) Extracted by use of Maxwell® RSC Instrument (Promega, Denmark) using Maxwell® RSC Cultured Cells DNA kit (AS 1620) (Promega, Denmark). Subsequently

(369) BL-MOL-AR Project, Preliminary Results about Liquid Biopsy: Molecular Approach Experience and Research Activity in Oncological SettingsGlobal Medical GeneticsSeptember 1, 2023Alessandro Pancrazzi, Francesco Bloise, Alice Moncada, Roberta Perticucci, Stefania Vecchietti, Francesca Pompili, Francesca Ricciarini, Silvia Lenzi, Cristina Gatteschi, Sabrina Giusti, et al.for purification of poor plasma. 20 21 The cfDNA extraction was performed with the Maxwell RSC Extractor, code AS4500, using the Maxwell RSC ccfDNA Plasma Kit, code AS1480 (Promega SRL, Milan, Italy). 22 The amount of

(370) Precision metagenomics sequencing for food safety: hybrid assembly of Shiga toxin-producing Escherichia coli in enriched agricultural waterFrontiers in MicrobiologyAugust 31, 2023Meghan Maguire, Padmini Ramachandran, Sandra Tallent, Mark K. Mammel, Eric W. Brown, Marc W. Allard, Steven M. Musser, Narjol González-Escalonacontaminated sample, Water and Water + Ecoli1–5, using the Maxwell RSC Cultured Cells DNA kit with a Maxwell RSC Instrument (Promega Corporation, Madison, WI) according to the manufacturer's instructions for Gram-negative bacteria

(371) The relation of ApoE and COMT gene–gene interactions to cognitive and motor function in community-dwelling older adults: a pilot studyFrontiers in Aging NeuroscienceAugust 31, 2023Kendra L. Pizzonia, Julie A. Suhr, Leatha A. Clark, Brian C. ClarkNucleic Acid Extraction instrument (Promega Corporation, 2,800 Woods Hollow Road Madison, WI; Cat. #AS4500) and Maxwell® RSC Buccal Swab DNA Kit (Cat. #AS1640) as we have previously described (Moskowitz et al.,

(372) Identification of an ADAMTS2 frameshift variant in a cat family with Ehlers–Danlos syndromeG3: Genes, Genomes, GeneticsAugust 30, 2023Rebecca Simon, Sarah Kiener, Nina Thom, Laura Schäfer, Janina Müller, Elfi K Schlohsarczyk, Ulrich Gärtner, Christiane Herden, Tosso Leeb, Gesine LühkenDNA was isolated from muscle tissue of the deceased kitten using a Maxwell RSC Tissue DNA Kit and a Maxwell RSC instrument (Promega, Dübendorf, Switzerland). For genotyping, DNA extraction from buccal swaps (sterile transport

(373) Investigations into SARS-CoV-2 and other coronaviruses on mink farms in France late in the first year of the COVID-19 pandemicPLoS OneAugust 25, 2023Marine Wasniewski, Franck Boué, Céline Richomme, Etienne Simon-Lorière, Sylvie Van der Werf, Flora Donati, Vincent Enouf, Yannick Blanchard, Véronique Beven, Estelle Leperchois, et al.of lysis buffer. RNA was extracted with the Maxwell RSC PureFood GMO and Authentication kit using a Maxwell RSC instrument (Promega France, Charbonnières les Bains, France), according to the manufacturer’s instructions. RNA

(374) Real-World Data on Combined EGFR-TKI and Crizotinib Treatment for Acquired and De Novo MET Amplification in Patients with Metastatic EGFR-Mutated NSCLCInternational Journal of Molecular SciencesAugust 23, 2023Edyta M. Urbanska, Morten Grauslund, Peter R. Koffeldt, Sarah L. B. Truelsen, Johan O. Löfgren, Junia C. Costa, Linea C. Melchior, Jens B. Sørensen, Eric Santoni-Rugiumanufacturer’s instructions. RNA was purified from diagnostic biopsies and tumor rebiopsies utilizing the Maxwell RSC instrument (Promega, Madison, WI, USA) with a Maxwell RSC RNA FFPE kit (Promega) and quantified with the Qubit™

(375) Manipulation of components of the renin angiotensin system in renal proximal tubules fails to alter atherosclerosis in hypercholesterolemic miceFrontiers in Cardiovascular MedicineAugust 16, 2023Masayoshi Kukida, Naofumi Amioka, Dien Ye, Hui Chen, Jessica J. Moorleghen, Ching-Ling Liang, Deborah A. Howatt, Yuriko Katsumata, Motoko Yanagita, Hisashi Sawada, et al.of the renal cortex was extracted using a commercial kit (Cat # AS1340, Promega) configured with a Maxwell RSC Instrument (Promega). To quantify mRNA abundance, total RNA was reversely transcribed with an iScript™ cDNA Synthesis kit

(376) Trade-offs between immunity and competitive ability in fighting ant malesBMC Ecol EvolAugust 7, 2023Sina Metzler, Jessica Kirchner, Anna V Grasse, Sylvia Cremertranscribed to cDNA before performing gene expression analysis using ddPCR. Total RNA was extracted using the Maxwell® RSC instrument together with the Maxwell® simplyRNA Tissue Kit (Promega) according to the manufacturer’s instructions

(377) Concordance in detection of microsatellite instability by PCR and NGS in routinely processed tumor specimens of several cancer typesCancer MedicineAugust 1, 2023Stephan Bartels, Isabel Grote, Madeleine Wagner, Jannik Boog, Elisa Schipper, Tanja Reineke‐Plaass, Hans Kreipe, Ulrich Lehmannfrom serial unstained sections. DNA isolation was performed with the Maxwell© RSC DNA FFPE kit on a Maxwell© RSC instrument (Promega, Madison). Measurement of the DNA concentration was performed with dsDNA high sensitivity kit

(378) Comparative Genetic Characterization of CTX-M-Producing Escherichia coli Isolated from Humans and Pigs with Diarrhea in Korea Using Next-Generation SequencingMicroorganismsJuly 28, 2023Kwang-Won Seo, Kyung-Hyo Do, Wan-Kyu LeeExtraction and Quantification Genomic DNA from 24 CTX-M-producing E. coli was extracted using the Maxwell RSC Instrument (Promega, Madison, WI, USA) and Maxwell® RSC Cultured Cells DNA Kit AS1620 (Promega), according to the

(379) Genomic and functional portrait of multidrug-resistant, hydrogen sulfide (H2S)-producing variants of Escherichia coliFrontiers in MicrobiologyJuly 27, 2023Razib Mazumder, Arif Hussain, Mohammad Mustafizur Rahman, Jody E. Phelan, Susana Campino, Ahmed Abdullah, Taane G. Clark, Dinesh Mondalclasses (Magiorakos et al., 2012). Whole-genome sequencing Total bacterial DNA was extracted using the Maxwell RSC Instrument and Culture Cell DNA extraction Kit (Promega) for gram-negative bacteria with an additional RNaseA treatment

(380) RALGAPA1 Deletion in Belgian Shepherd Dogs with Cerebellar AtaxiaGenes (Basel)July 25, 2023Matthias Christen, Isabel Zdora, Michael Leschnik, Vidhya Jagannathan, Christina Puff, Enrice Hünerfauth, Holger A. Volk, Wolfgang Baumgärtner, Tessa C. Koch, Wencke Schäfer, et al.Extraction We extracted genomic DNA from EDTA blood samples with a Maxwell RSC whole blood DNA kit using a Maxwell RSC instrument (Promega, Madison, WI, USA). A Maxwell RSC DNA FFPE kit was used for DNA isolation from FFPE tissue

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