Maxwell RSC Instrument from Promega

Product Specs
ItemMaxwell RSC Instrument
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS4500
QuantityEA
Processing Time30 to 60 min (depending on sample type and method)
CapacityUp to 16
Dimensions(WxDxH) 330.2 x 345.2 x 299.7 mm (13 x 13.6 x 11.8 in)
UV LampAvg. lifetime approximately 6,000 hrs
Molecular Weight~85kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(361) Salivary miRNA Profiles in COVID-19 Patients with Different Disease SeveritiesInternational Journal of Molecular SciencesJuly 1, 2023Irma Saulle, Micaela Garziano, Gioia Cappelletti, Fiona Limanaqi, Sergio Strizzi, Claudia Vanetti, Sergio Lo Caputo, Mariacristina Poliseno, Teresa Antonia Santantonio, Mario Clerici, et al.using the Maxwell RSC Viral Total Nucleic Acid Purification Kit (Promega, Fitchburg, WI, USA) with the Maxwell® RSC Instrument (Promega, Fitchburg, WI, USA), as previously described [51]. Briefly, single-step, real-time RT-qPCR

(362) TXNIP contributes to induction of pro-inflammatory phenotype and caspase-3 activation in astrocytes during Alzheimer's diseasesRedox biologyJuly 1, 2023Kim J, Lim J, Yoo ID, Park S, Moon JS.(AS1340, Promega), or Maxwell® RSC simplyRNA cells kit (AS1390, Promega) with Maxwell® RSC Instrument (AS4500, Promega). Amounts of RNA were measured with QuantiFluor® RNA System (E3310, Promega) using Quantus™ Fluorometer

(363) First Report of Olive Branch Dieback in Croatia Caused by Cytospora pruinosa DéfagoMicroorganismsJune 28, 2023Elena Petrović, Karolina Vrandečić, Dario Ivić, Jasenka Ćosić, Sara Godenamargins and used for genomic DNA extraction. Total genomic DNA from the isolate was extracted using a Maxwell® RSC Instrument (Promega, Madison, WI, USA) and Maxwell® RSC Plant DNA Kit (Promega, Madison, WI, USA). The nuclear

(364) Autosomal recessive hyposegmentation of granulocytes in Australian Shepherd Dogs indicates a role for LMBR1L in myeloid leukocytesPLOS GeneticsJune 22, 2023Bianca Lourdes Frehner, Matthias Christen, Iris M. Reichler, Vidhya Jagannathan, Marilisa Novacco, Barbara Riond, Laureen M. Peters, José Suárez Sánchez-Andrade, Aldona Pieńkowska-Schelling, Claude Schelling, et al.extraction Genomic DNA was isolated from EDTA blood with the Maxwell RSC Whole Blood Kit, using a Maxwell RSC instrument (Promega, Dübendorf, Switzerland). Genomic DNA from liver tissue samples of the stillborn puppies was

(365) A Comparison of Two Different FFPE Tissue Dissection Methods for Routine Diagnostics in Molecular Pathology: Manual Macrodissection versus Automated Microdissection Using the Roche “AVENIO Millisect” SystemCancers (Basel)June 20, 2023Jan Jeroch, Tobias Riedlinger, Christina Schmitt, Silvana Ebner, Ria Winkelmann, Peter J. Wild, Melanie Demesdissection methods was digested with proteinase K and the nucleic acid was extracted with the help of the MaxWell® RSC instrument (Promega, Madison, WI, USA) and the Maxwell® FFPE Plus LEV DNA Purification Kit (Promega, Madison, WI,

(366) Prevalence of Enterococcus spp. and the Whole-Genome Characteristics of Enterococcus faecium and Enterococcus faecalis Strains Isolated from Free-Living Birds in PolandPathogensJune 16, 2023Renata Kwit, Magdalena Zając, Aleksandra Śmiałowska-Węglińska, Magdalena Skarżyńska, Arkadiusz Bomba, Anna Lalak, Ewelina Skrzypiec, Dominika Wojdat, Weronika Koza, Emilia Mikos-Wojewoda, et al.Cultured Cells (AS1620 Promega, Madison, WI, USA), according to the manufacturer’s instructions, with a Maxwell® RSC Instrument (Promega, Madison, WI, USA). Sequencing libraries were prepared with KAPA HyperPlus (Roche, Pleasanton,

(367) Detection of circulating cell-free HPV DNA of 13 HPV types for patients with cervical cancer as potential biomarker to monitor therapy response and to detect relapseBritish Journal of CancerJune 15, 2023Suzana Mittelstadt, Olga Kelemen, Jakob Admard, Axel Gschwind, André Koch, Sarah Wörz, Ernst Oberlechner, Tobias Engler, Irina Bonzheim, Annette Staebler, et al.necessary. Genomic DNA was extracted from the sections using the Maxwell® RSC DNA FFPE Kit and the Maxwell® RSC Instrument (Promega, Madison, WI, USA) according to the manufacturer’s instructions. Next-Generation Sequencing

(368) Circulating miR-26b-5p and miR-451a as diagnostic biomarkers in medullary thyroid carcinoma patientsJournal of Endocrinological InvestigationJune 7, 2023Z. M. Besharat, S. Trocchianesi, A. Verrienti, R. Ciampi, S. Cantara, C. Romei, C. Sabato, T. M. R. Noviello, A. Po, A. Citarella, et al.pEV samples RNA was extracted using PROMEGA Maxwell RSC miRNA Plasma and Serum kit (#AS1680) by the Maxwell® RSC Instrument according to manufacturer’s instruction. Extracted RNA was eluted in 30 μl of H2O RNase-free. Prior

(369) Establishment of wastewater-based SARS-CoV-2 monitoring system over two years: Case studies in South KoreaJournal of Environmental Chemical EngineeringJune 5, 2023Lan Hee Kim, Viktorija Mikolaityte, Sungpyo Kimvortexed for 10 s. The extractants were incubated for 10 min at 56 °C, and the RNA was extracted using a Maxwell® RSC instrument (Promega, USA). For the sewage sludge samples, the 0.5 g of sludge was placed in a 1.5 mL microtube

(370) Epidemiology and zoonotic transmission of mcr-positive and carbapenemase-producing Enterobacterales on German turkey farmsFrontiers in MicrobiologyJune 2, 2023Katja Nordhoff, Martina Scharlach, Natalie Effelsberg, Carolin Knorr, Dagmar Rocker, Katja Claussen, Richard Egelkamp, Alexander C. Mellmann, Andreas Moss, Ilona Müller, et al.these cultures were used for DNA extraction. Genomic DNA of E. coli isolates was extracted with the Maxwell® RSC Instrument (an automated nucleic acid purification platform) using the Maxwell® RSC Whole Blood DNA Kit (Promega,

(371) PKD1 Nonsense Variant in a Lagotto Romagnolo Family with Polycystic Kidney DiseaseGenes (Basel)June 1, 2023Michaela Drögemüller, Nadine Klein, Rikke Lill Steffensen, Miriam Keiner, Vidhya Jagannathan, Tosso LeebBiobank, were used. Genomic DNA was isolated from EDTA blood using the Maxwell RSC Whole Blood Kit on a Maxwell RSC instrument (Promega, Dübendorf, Switzerland). 2.3. Whole Genome Sequencing (WGS) Three Illumina TruSeq PCR-free

(372) Cell-free DNA as a potential biomarker of differentiation and toxicity in cardiac organoidseLifeJune 1, 2023Silver B, Gerrish K, Tokar E.Kit AS1610) and cfDNA from media (Promega, ccfDNA Plasma Kit AS1480) were extracted using the Promega Maxwell RSC instrument. RNA quality and purity were assessed using a NanoDrop 2000 spectrophotometer, and DNA concentrations

(373) Two sporadic cases of childhood-onset Hailey-Hailey disease with superimposed mosaicismEuropean Journal of Human GeneticsJune 1, 2023Yasuhiko Asahina, Umi Tahara, Satomi Aoki, Kazuhiko Nakabayashi, Chiharu Tateishi, Daisuke Hayashi, Masayuki Amagai, Daisuke Tsuruta, Akiharu Kubowas collected. Genomic DNA was obtained from the epidermis and peripheral blood leukocytes using a Maxwell RSC Instrument and Maxwell RSC Blood DNA Kit (Promega, Madison, WI). Targeted exome sequencing Targeted exome sequencing,

(374) IPSC reprogramming of two patients with spondyloepiphyseal dysplasia congenita (SEDC)Stem Cell ResearchJune 1, 2023Pauline De Kinderen, Laura Rabaut, Melanie H.A.M. Perik, Silke Peeters, Peter Ponsaerts, Bart Loeys, Geert Mortier, Josephina A.N. Meester, Aline Verstraetenanalysis) Genomic DNA of patient PBMCs and the resulting iPSCs (passage 12) was isolated using the Maxwell® RSC Instrument and Maxwell® RSC Cultured Cells DNA Kit (Promega). Subsequently, a HumanCytoSNP-12 assay (Illumina)

(375) Performance of the procedure for ultra-rapid extraction and loop-mediated isothermal amplification (PURE-LAMP) method to detect malaria in HaitiInfectious Diseases of PovertyMay 22, 2023Jeanne Perpétue Vincent, Alexandre Valcena Existe, Kanako Komaki-Yasuda, Jacques Boncy, Shigeyuki Kanoa diameter of 3 mm using a Maxwell® RSC DNA FFPE Kit (Promega, Madison, WI, USA) and the automated Maxwell® RSC Instrument (Promega, Madison, WI, USA). Minor modifications to the manufacturer’s guidelines can be found in a

(376) RNA-seq analysis to identify genes related to resting egg production of panarctic Daphnia pulexBMC GenomicsMay 17, 2023Natsumi Maruoka, Takashi Makino, Jotaro Urabelonger observed. RNA was extracted using a Maxwell RSC Plant RNA Kit with a Maxwell® RSC Instrument AS4500 (Promega, Madison, WI, USA) following the manufacturer’s protocol and RNA concentrations were measured

(377) Biomolecular Monitoring Tool Based on Lab-on-Chip for Virus DetectionBiosensors (Basel)May 12, 2023Francesca Costantini, Nicola Lovecchio, Manasa Nandimandalam, Ariana Manglli, Francesco Faggioli, Mara Biasin, Cesare Manetti, Pio Federico Roversi, Augusto Nascetti, Giampiero de Cesare, et al.performed on VeroE6 cells, and RNA was extracted from six days post-infection (dpi) supernatants using the Maxwell® RSC Instrument with Maxwell® RSC Viral Total Nucleic Acid Purification (Promega, Fitchburg, WI, USA). The RNA extracts

(378) A Real-World Experience from a Single Center (LPCE, Nice, France) Highlights the Urgent Need to Abandon Immunohistochemistry for ROS1 Rearrangement Screening of Advanced Non-Squamous Non-Small Cell Lung CancerJournal of Personalized MedicineMay 10, 2023Véronique Hofman, Samantha Goffinet, Christophe Bontoux, Elodie Long-Mira, Sandra Lassalle, Marius Ilié, Paul Hofmanpositive. 2.2. Next Generation Sequencing The nucleic acid extraction was conducted using either the Maxwell RSC Instrument (Promega, Madison, WI, USA) in combination with the Maxwell RSC FFPE Plus DNA kit or the Maxwell RSC RNA FFPE kit

(379) New, fast and cheap prediction tests for BRCA1 gene mutations identification in clinical samplesScientific ReportsMay 5, 2023Aleksandra Gajda-Walczak, Agnieszka Potęga, Agata Kowalczyk, Slawomir Sek, Sebastian Zięba, Artur Kowalik, Andrzej Kudelski, Anna M. Nowickaisolated from 300 μL whole blood with Maxwell® RSC Whole Blood DNA Kit (Promega, Madison, WI, USA) using Maxwell® RSC Instrument (Promega, Madison, WI, USA) according to manufacturer protocol. Two microliters of isolated DNA were

(380) The DNA integrity number and concentration are useful parameters for successful comprehensive genomic profiling test for cancer using formalin‐fixed paraffin embedded tissuePathology InternationalMay 1, 2023Emmy Yanagita, Hiroshi Yamada, Tetsuro Kobayashi, Eriko Aimono, Kohei Nakamura, Akira Hirasawa, Hiroshi Nishihara3 Target amplicon sequence Genomic DNA, which was extracted from thin sliced FFPE tissue using the Maxwell RSC Instrument（Promega), was applied for library construction with the GeneRead DNAseq Targeted Panels V2 (Human Comprehensive

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