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Automated DNA/RNA Purification Sample Preparation Workstations
Maxwell RSC Instrument
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Maxwell RSC Instrument
Maxwell RSC Instrument
Maxwell RSC Instrument from Promega
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Citations:
(849)
Figures:
(1)
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Maxwell RSC Instrument
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Description
The Maxwell RSC Instrument is a platform for automated extraction of nucleic acid from a range of sample types. Extraction methods use sample lysis and binding to paramagnetic particles followed by washing steps and elution.
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Maxwell RSC Instrument
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
AS4500
Quantity
EA
Processing Time
30 to 60 min (depending on sample type and method)
Capacity
Up to 16
Dimensions
(WxDxH) 330.2 x 345.2 x 299.7 mm (13 x 13.6 x 11.8 in)
UV Lamp
Avg. lifetime approximately 6,000 hrs
Molecular Weight
~85kDa
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Promega
2800 Woods Hollow Rd
Madison
,
WI
53711
United States
Phone
: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516
Email: custserv@promega.com
techserv@promega.com
Company Profile
Website:
http://www.promega.com
Citations (849)
(281)
Target enrichment metaviromics enables comprehensive surveillance of coronaviruses in environmental and animal samples
Heliyon
June 15, 2024
Sandra Martínez-Puchol, Maria Tarradas-Alemany, Cristina Mejías-Molina, Marta Itarte, Marta Rusiñol, Jordi Baliellas, Nerea Abasolo, Núria Canela, Abir Monastiri, Marc López-Roig,
et al.
Enviro Wastewater TNA Kit (
Promega
, Wisconsin, USA), the VacMan® Vacuum Manifold and the automated
Maxwell RSC instrument
into a final volume of 60 μL. From all the samples tested,
SARS-CoV-2
N1 gene quantification was performed
(282)
Shifting from Immunohistochemistry to Screen for ALK Rearrangements: Real-World Experience in a Large Single-Center Cohort of Patients with Non-Small-Cell Lung Cancer
Cancers (Basel)
June 14, 2024
Marius Ilié, Samantha Goffinet, Guylène Rignol, Virginie Lespinet-Fabre, Salomé Lalvée, Olivier Bordone, Katia Zahaf, Christelle Bonnetaud, Kevin Washetine, Sandra Lassalle,
et al.
routine clinical practice (www.cofrac.fr, accessed on 15 March 2024). Nucleic acids were extracted using
Maxwell RSC Instrument
(
Promega
, Madison, WI, USA) with the
Maxwell RSC FFPE Plus DNA kit
or the
Maxwell RSC RNA FFPE kit
(
Promega
).
(283)
Evaluating Single-Nucleotide Polymorphisms in Inflammasome Proteins and Serum Levels of IL-18 and IL-1β in Kidney Interstitial Damage in Anti-Neutrophilic Cytoplasmic Antibody-Associated Vasculitis
International journal of molecular sciences
June 12, 2024
Martinez Valenzuela L, Vidal-Alabró A, Rubio B, Antón-Pàmpols P, Gómez-Preciado F, Fulladosa X, Cruzado JM, Torras J, Lloberas N, Draibe J.
recruitment. Genomic deoxyribonucleic acid (DNA) was extracted from the peripheral whole blood of patients using a
Maxwell RSC® instrument
(
Promega Corporation
, Sydney, Australia). The DNA concentration and purity (260/280) were checked using
(284)
Cannabinerol Prevents Endoplasmic Reticulum and Mitochondria Dysfunctions in an In Vitro Model of Alzheimer’s Disease: A Network-Based Transcriptomic Analysis
Cells
June 10, 2024
Luigi Chiricosta, Aurelio Minuti, Agnese Gugliandolo, Stefano Salamone, Federica Pollastro, Emanuela Mazzon, Osvaldo Artimagnella
extracted using the
Maxwell® RSC simplyRNA Cells Kit
(#
AS1390
,
Promega
, Madison, WI, USA) with the
Maxwell® RSC instrument
. Afterwards, library preparation (from 100 ng of total RNA) of two biological replicates was carried
(285)
Enterovirus A71 crosses a human blood–brain barrier model through infected immune cells
Microbiology Spectrum
June 4, 2024
Léa Gaume, Hélène Chabrolles, Maxime Bisseux, Igor Lopez-Coqueiro, Lucie Dehouck, Audrey Mirand, Cécile Henquell, Fabien Gosselet, Christine Archimbaud, Jean-Luc Bailly
nucleic acids were extracted from 200 µL of infected hBLEC samples with the Maxwell RSC Blood Kit and
Maxwell RSC instrument
(
Promega
). The quantification of viral genomes in the samples was performed by the commercial semi-quantitative
(286)
Novel Ralstonia species from human infections: improved matrix-assisted laser desorption/ionization time-of-flight mass spectrometry-based identification and analysis of antimicrobial resistance patterns
Microbiology Spectrum
June 4, 2024
Stephanie Steyaert, Charlotte Peeters, Anneleen D. Wieme, Astrid Muyldermans, Kristof Vandoorslaer, Theodore Spilker, Ingrid Wybo, Denis Piérard, John J. LiPuma, Peter Vandamme
using the
Maxwell RSC Cultured Cells DNA kit
(
AS1620
,
Promega
, USA) and the
Maxwell RSC instrument
(
AS4500
,
Promega
, USA) according to the manufacturer’s instructions, except for the final step, in which the
(287)
Neuroinflammation and Lysosomal Abnormalities Characterise the Essential Role for Oxidation Resistance 1 in the Developing and Adult Cerebellum
Antioxidants (Basel)
June 3, 2024
Eboni M. V. Bucknor, Errin Johnson, Stephanie Efthymiou, Javeria R. Alvi, Tipu Sultan, Henry Houlden, Reza Maroofian, Ehsan G. Karimiani, Mattéa J. Finelli, Peter L. Oliver
above. 2.5. RNA Extraction,
cDNA Synthesis
and
qPCR
Total RNA was extracted from cerebellar tissue using the
Maxwell RSC Instrument
and simplyRNA Tissue Kit (
Promega
, Madison, WI, USA) as per manufacturer’s instructions.
RNA quantification
(288)
A phased genome of the highly heterozygous ‘Texas’ almond uncovers patterns of allele-specific expression linked to heterozygous structural variants
Horticulture Research
June 3, 2024
Raúl Castanera, Carlos de Tomás, Valentino Ruggieri, Carlos Vicient, Iban Eduardo, Maria José Aranzana, Pere Arús, Josep M Casacuberta
chloroform
centrifugation
step. Total RNA was extracted using the
Maxwell RSC Plant RNA Kit
and the
Maxwell RSC instrument
(
Promega Corporation
, Madison, WI, USA). Complete DNA removal was obtained using the
DNA-free
DNA Removal
(289)
Improved prognostic stratification of patients with isocitrate dehydrogenase-mutant astrocytoma
Acta Neuropathologica
June 1, 2024
Michael Weller, Jörg Felsberg, Bettina Hentschel, Dorothee Gramatzki, Nadezhda Kubon, Marietta Wolter, Matthias Reusche, Patrick Roth, Dietmar Krex, Ulrich Herrlinger,
et al.
Germany), the
GeneRead DNA FFPE Kit
(
Qiagen
), or the
Maxwell® RSC FFPE Plus DNA Kit
together with the
Maxwell® RSC instrument
(
Promega
, Mannheim, Germany).
Tumor
tissue samples used for DNA extraction were histologically evaluated
(290)
Inborn errors of type I interferon immunity in patients with symptomatic acute hepatitis E
Hepatology
June 1, 2024
Ali Saadat, Jérôme Gouttenoire, Paolo Ripellino, David Semela, Soraya Amar, Beat M. Frey, Stefano Fontana, Elise HEV Human Genetics Collaborators, Darius Mdawar-Bailly, Jacques Moradpour,
et al.
participant. DNA was extracted using the DNeasy Blood and Tissue Kit from Qiagen (Hilden, Germany) or on the
Maxwell RSC Instrument
using the Maxwell RSC DNA Blood Kit (
Promega
, Madison, WI). DNA concentration was measured on Qubit
(291)
Beneficial effect of heat-killed Lactiplantibacillus plantarum L-137 on intestinal barrier function of rat small intestinal epithelial cells
Scientific Reports
May 29, 2024
Mototsugu Watanabe, Hiroko Nakai, Tatsuya Ohara, Kengo Kawasaki, Shinji Murosaki, Yoshitaka Hirose
Maxwell® RSC simplyRNA Cells Kit
(
Promega
, Madison, WI, USA) and automated RNA extraction system by using the
Maxwell® RSC Instrument
(
Promega
) according to the manufacturer’s instructions37–39. The RNA was eluted with 50 μL of nuclease-free
(292)
Detection Method for Gene Doping in a Mouse Model Expressing Human Erythropoietin from Adeno-Associated Virus Vector-9
Genes (Basel)
May 29, 2024
Takehito Sugasawa, Atsushi Hirokawa, Norihiro Otani, Yasuharu Kanki, Kieu DM Nguyen, Tohru Takemasa, Koichi Watanabe, Yoshinori Takeuchi, Naoya Yahagi, Yoichiro Takahashi
total DNA from 100 µL (short-term experiment) or 50 µL (long-term experiment) of whole blood, using a
Maxwell RSC Instrument
(
Promega
), according to the manufacturer’s instructions. The DNAs were dissolved in 50 µL of Milli-Q
(293)
Field validation of an eDNA assay for nutria illuminates a role in invasive species management
Ecology and Evolution
May 23, 2024
Stephanie S. Coster
and subjected to
centrifugation
for 10 min. at 16,000 g and the supernatant was used downstream in a
Maxwell RSC Instrument
(
Promega
) with the
Maxwell RSC PureFood GMO and Authentication Kit
(Promega) following the recommended
(294)
Capsid Integrity Detection of Enteric Viruses in Reclaimed Waters
Viruses
May 21, 2024
Pablo Puchades-Colera, Azahara Díaz-Reolid, Inés Girón-Guzmán, Enric Cuevas-Ferrando, Alba Pérez-Cataluña, Gloria Sánchez
Quantification Nucleic acid extraction from influent wastewater and reclaimed water concentrates was performed using the
Maxwell® RSC Instrument
(
Promega
, Madison, WI, USA) with the Maxwell RSC Pure Food GMO and authentication kit (Promega) and
(295)
Isolation and Characterization of Cell-Free DNA from Cerebral Organoids
International Journal of Molecular Sciences
May 18, 2024
Brian B. Silver, Ashley Brooks, Kevin Gerrish, Erik J. Tokar
Gene Expression
Genomic DNA (gDNA) from
organoids
and cfDNA from conditioned media were extracted using the
Promega
Maxwell® RSC instrument
(Promega, Madison, WI, USA) with the Tissue DNA Kit or ccfDNA Plasma Kit, respectively. DNA concentrations
(296)
High-throughput surface epitope immunoaffinity isolation of extracellular vesicles and downstream analysis
Biology Methods and Protocols
May 17, 2024
Ramin Khanabdali, Michelle Mandrekar, Rick Grygiel, Phuoc-An Vo, Carlos Palma, Sara Nikseresht, Siena Barton, Mozhgan Shojaee, Sadman Bhuiyan, Kartini Asari,
et al.
analyses, bead-captured EVs were lysed with 1% SDS. SEI isolation of EVs was also implemented on the
Maxwell RSC instrument
(
Promega
) for medium-throughput sample processing (see Supplementary Fig. S1). EV isolation method
(297)
Closing the gap: Oxford Nanopore Technologies R10 sequencing allows comparable results to Illumina sequencing for SNP-based outbreak investigation of bacterial pathogens
Journal of Clinical Microbiology
May 8, 2024
Bert Bogaerts, An Van den Bossche, Bavo Verhaegen, Laurence Delbrassinne, Wesley Mattheus, Stéphanie Nouws, Maxime Godfroid, Stefan Hoffman, Nancy H. C. Roosens, Sigrid C. J. De Keersmaecker,
et al.
described elsewhere (24, 25). The DNA extraction of the
E. coli
ONT data sets was performed using the
Maxwell RSC Instrument
(
Promega
, WI, USA) according to the manufacturer’s instructions. For the Illumina and ONT
sequencing
(298)
The stress-responsive cytotoxic effect of diesel exhaust particles on lymphatic endothelial cells
Scientific Reports
May 7, 2024
Yu Sakurai, Eiki Oba, Akiko Honda, Hiroki Tanaka, Hirohisa Takano, Hidetaka Akita
PBS, and then total RNA was extracted from the cells using the
Maxwell RSC simplyRNA Tissue Kit
and
Maxwell RSC Instrument
(
Promega
, Madison, WI, U.S.A). The extracted RNAs from 3 samples individually treated with each concentration
(299)
An Evaluation of High-Risk HPV in Squamous Cell Carcinomas of the Lip in a South African Cohort
Head and Neck Pathology
May 6, 2024
Sharon N. Harbor, Johann W. Schneider, Nadine Solomons, Micheline Sanderson, Amir H. Afrogheh
Total RNA was extracted from samples using the
Maxwell® RSC RNA FFPE kit
(
Promega
, Wisconsin, USA) and
Maxwell RSC instrument
(
Promega
, Wisconsin, USA), following the manufacturer’s instructions. RNA quality was assessed using
(300)
Acute Lymphoblastic Leukemia with Myeloid Mutations Is a High-Risk Disease Associated with Clonal Hematopoiesis
Blood Cancer Discovery
May 1, 2024
Caner Saygin, Pu Zhang, Jacob Stauber, Ibrahim Aldoss, Adam S. Sperling, Lachelle D. Weeks, Marlise R. Luskin, Todd C. Knepper, Pankhuri Wanjari, Peng Wang,
et al.
extracted from peripheral blood or
bone
marrow collected in EDTA tubes using the simplyRNA Blood Kit on
Maxwell RSC instrument
(
Promega
) and quantified using a Qubit fluorometric
assay
(Thermo Fisher Scientific), adjusted for the
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Maxwell RSC Instrument from Promega
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