Maxwell RSC Instrument from Promega

Product Specs
ItemMaxwell RSC Instrument
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS4500
QuantityEA
Processing Time30 to 60 min (depending on sample type and method)
CapacityUp to 16
Dimensions(WxDxH) 330.2 x 345.2 x 299.7 mm (13 x 13.6 x 11.8 in)
UV LampAvg. lifetime approximately 6,000 hrs
Molecular Weight~85kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(281) Gut Microbiome and Metabolic and Immune Indices in Males with or without Evidence of Metabolic DysregulationJournal of Obesity & Metabolic SyndromeMarch 30, 2024Kyle M. Hatton-Jones, Nicholas P. West, Mike W.C. Thang, Pin-Yen Chen, Peter Davoren, Allan W. Cripps, Amanda J. Coxstored in PAXgene Blood RNA (BD Biosciences) tubes was thawed and RNA was isolated using the automated Maxwell RSC Instrument (Promega) and the RSC simplyRNA tissue kit (Promega). Isolated RNA was analysed using both the commercially

(282) Comparative Analyses of Targeted Myeloid Cancer Next-Generation Sequencing Panel in Fresh Blood, Bone Marrow and FFPE MaterialInt J Mol SciMarch 21, 2024Andrea Daniela Hobeck, Sophia Wendt, Saskia Krohn, Gudrun Knuebel, Stephan Bartels, Elisa Schipper, Christian Junghanss, Hugo Murua EscobarHannover Medical School, Germany. FFPE samples were prepared with the Maxwell© RSC DNA FFPE kit on a Maxwell© RSC instrument (Promega, Fitchburg, MA, USA) for gDNA isolation, and quantification was performed with a Qubit™ dsDNA HS Assay Kit

(283) A single workflow for multi-species blood transcriptomicsBMC GenomicsMarch 16, 2024Elody Orcel, Hayat Hage, May Taha, Noémie Boucher, Emilie Chautard, Virginie Courtois, Adrien Saliouthe RNA extraction and the library preparation. Our extraction protocol is already integrated on the Maxwell RSC Instrument (Promega). Conclusions We recommend the following workflow for blood transcriptomics analysis: (i)

(284) Betanin Attenuates Epigenetic Mechanisms and UV-Induced DNA Fragmentation in HaCaT Cells: Implications for Skin Cancer ChemopreventionNutrientsMarch 16, 2024Afshin Zand, Sodbuyan Enkhbilguun, John M. Macharia, Krisztina Varajti, Istvan Szabó, Gellért Gerencsér, Boglárka Bernadett Tisza, Bence L. Raposa, Zoltán Gyöngyi, Timea VarjasQuantitative Real-Time PCR Analysis Total RNA was isolated via a semiautomatic workflow with the help of the Maxwell® RSC Instrument (Promega, Fitchburg, WI, USA) using a Maxwell® RSC RNA FFPE Kit (AS1440, Promega, Fitchburg, WI, USA).

(285) First Detection of Benign Rabbit Caliciviruses in ChileVirusesMarch 12, 2024Elena Smertina, Luca M. Keller, Nina Huang, Gabriela Flores-Benner, Jennifer Paola Correa-Cuadros, Melanie Duclos, Fabian M. Jaksic, Cristóbal Briceño, Victor Neira Ramirez, Miguel Díaz-Gacitúa, et al.homogeniser (Bertin Technologies, Montigny-le-Bretonneux, France). Total RNA was extracted using the Maxwell® RSC instrument (Promega, Madison, WI, USA) in combination with the Maxwell® RSC SimplyRNA Tissue Kit (Promega, Madison,

(286) Leveraging Plasma-Activated Seawater for the Control of Human Norovirus and Bacterial Pathogens in Shellfish DepurationFoodsMarch 11, 2024Annamaria Pandiscia, Patrizio Lorusso, Alessio Manfredi, Gloria Sánchez, Valentina Terio, Walter Randazzomonolayers at 1 and 48 hpi to evaluate HuNoV replication. Briefly, viral RNA was extracted using the Maxwell® RSC Instrument (Promega, Madrid, Spain) according to the manufacturer’s instructions. Then, it was detected in duplicate

(287) Screening for cold tolerance genes in C. elegans, whose expressions are affected by anticancer drugs camptothecin and leptomycin BSci RepMarch 5, 2024Misaki Okahata, Natsumi Sawada, Kenji Nakao, Akane Ohta, Atsushi Kuhara3 h. The Maxwell RSC Simply RNA tissue kit (Promega; AS1340) with Maxwell RSC Instrument (Promega; AS4500) was used to extract total RNA in accordance with the manufacturer’s instructions. Sequencing libraries

(288) Microenvironment‐induced restoration of cohesive growth associated with focal activation of P‐cadherin expression in lobular breast carcinoma metastatic to the colonThe Journal of Pathology: Clinical ResearchMarch 1, 2024Malte Gronewold, Isabel Grote, Stephan Bartels, Henriette Christgen, Leonie D Kandt, Maria Jose Brito, Gàbor Cserni, Maximilian E Daemmrich, Franz Fogt, Burkhard M Helmke, et al.brief, genomic DNA was extracted with the Maxwell RSC DNA FFPE kit (Promega, Madison, WI, USA) on a Maxwell RSC instrument (Promega). DNA was quantified using a Qubit 2.0 fluorometer (Invitrogen, Darmstadt, Germany) and the

(289) Combining Homologous Recombination-Deficient Testing and Functional RAD51 Analysis Enhances the Prediction of Poly(ADP-Ribose) Polymerase Inhibitor SensitivityJCO Precis OncolMarch 1, 2024Lea M. Korsholm, Maj Kjeldsen, Lorenzo Perino, Luca Mariani, Gitte-Bettina Nyvang, Elisabeth Kristensen, Frederik O. Bagger, Mansoor Raza Mirza, Maria Rossingusing the QIAsymphony GeneRead DNA FFPE Treatment Kit (Qiagen, Hilden, Germany) and purified using the Maxwell RSC instrument (Promega, Madison, WI). Extracted DNA was quantified using the Qubit Fluorometer (Thermo Fisher Scientific,

(290) Prediction of plasma ctDNA fraction and prognostic implications of liquid biopsy in advanced prostate cancerNature CommunicationsFebruary 28, 2024Nicolette M. Fonseca, Corinne Maurice-Dror, Cameron Herberts, Wilson Tu, William Fan, Andrew J. Murtha, Catarina Kollmannsberger, Edmond M. Kwan, Karan Parekh, Elena Schönlau, et al.fraction using the Qiagen (Germany) DNeasy Blood and Tissue Kit, or the Maxwell® RSC Blood DNA Kit and Maxwell® RSC Instrument (Promega, USA). Extracted gDNA was quantified with a NanoDrop spectrophotometer. Targeted DNA sequencing

(291) A Novel Postbiotic Product Based on Weissella cibaria for Enhancing Disease Resistance in Rainbow Trout: Aquaculture ApplicationAnimals (Basel)February 27, 2024Mario Quintanilla-Pineda, Francisco C. Ibañez, Chajira Garrote-Achou, Florencio Marzoindicated for the elimination of genomic DNA from samples. The cartridge was then placed on the tray of the Maxwell® RSC Instrument (Promega Corporation, Fitchburg, MA, USA). Finally, one elution tube of 0.5 mL per cartridge with 50

(292) Screening for Rare Mitochondrial Genome Variants Reveals a Potentially Novel Association between MT-CO1 and MT-TL2 Genes and Diabetes PhenotypeInternational Journal of Molecular SciencesFebruary 19, 2024Tomasz Płoszaj, Sebastian Skoczylas, Karolina Gadzalska, Paulina Jakiel, Ewa Juścińska, Monika Gorządek, Agnieszka Robaszkiewicz, Maciej Borowiec, Agnieszka ZmysłowskaPreparation and Sequencing Reaction DNA isolation was performed from peripheral blood with a semi-automatic Maxwell RSC Instrument (Promega, Madison, WI, USA). The library was prepared via the Agilent SureSelectQXT Target Enrichment

(293) Nuclear pore complex proteins are involved in centromere distributioniScienceFebruary 16, 2024Nanami Ito, Takuya Sakamoto, Yuka Oko, Hikaru Sato, Shigeru Hanamata, Yuki Sakamoto, Sachihiro Matsunagadetails qRT-PCR One-week-old plants were used for qRT-PCR. RNA was extracted using a Maxwell® RSC Plant RNA Kit (Promega) and Maxwell® RSC Instrument (Promega), and reverse-transcribed using a Verso cDNA Synthesis Kit (Thermo Scientific). Gene expression

(294) Multiple interspecies recombination events documented by whole-genome sequencing in multidrug-resistant Haemophilus influenzae clinical isolatesAccess MicrobiologyFebruary 12, 2024Charlotte Michel, Maria De Los Angeles Argudín, Magali Wautier, Fedoua Echahidi, Benoit Prevost, Olivier Vandenberg, Delphine Martiny, Marie HallinCO2 for 18 h +/−6 h as previously described [2]. WGS Genomic DNA extractions were performed on the Maxwell RSC instrument (Promega Corporation, Madison, WI, USA) with the Maxwell RSC Cell DNA purification kit. Genomic DNA

(295) Differential Photosensitivity of Fibroblasts Obtained from Normal Skin and Hypertrophic Scar TissuesInternational Journal of Molecular SciencesFebruary 9, 2024Junya Kusumoto, Masaya Akashi, Hiroto Terashi, Shunsuke SakakibaraHSFs. 4.2. Total RNA Extraction Total RNA was semi-automatically extracted from cultured cells using the Maxwell RSC Instrument (Promega, Madison, WI, USA) and the Maxwell RSC simplyRNA kit (AS1340, Promega), following the manufacturer’s

(296) Image‐guided metabolomics and transcriptomics reveal tumour heterogeneity in luminal A and B human breast cancer beyond glucose tracer uptakeClinical and Translational MedicineFebruary 8, 2024Qianlu Yang, Sisi Deng, Heike Preibsch, Tim‐Colin Schade, André Koch, Georgy Berezhnoy, Laimdota Zizmare, Anna Fischer, Brigitte Gückel, Annette Staebler, et al.was extracted from macro‐dissected 5 μm paraffin sections using the Maxwell® RSC RNA FFPE Kit and the Maxwell® RSC Instrument (Promega, Madison, WI, USA). The RNA samples were evaluated for yield and purity using a NanoDrop 2000 Spectrophotometer

(297) NDUFS7 variant in dogs with Leigh syndrome and its functional validation in a Drosophila melanogaster modelScientific ReportsFebruary 5, 2024Matthias Christen, Anne Gregor, Rodrigo Gutierrez-Quintana, Jos Bongers, Angie Rupp, Jacques Penderis, G. Diane Shelton, Vidhya Jagannathan, Christiane Zweier, Tosso LeebDNA was extracted from EDTA blood using the Maxwell RSC Whole Blood DNA kit in combination with the Maxwell RSC instrument (Promega, Dübendorf, Switzerland). An Illumina TruSeq PCR-free library with ~ 420 bp insert size was

(298) Genetic characterization of intramuscular myxomasPathology and Oncology ResearchJanuary 22, 2024William John Hatchett, Marta Brunetti, Kristin Andersen, Maren Randi Tandsæther, Ingvild Lobmaier, Marius Lund-Iversen, Thomas Lien-Dahl, Francesca Micci, Ioannis PanagopoulosS1). DNA was extracted from the frozen tumor samples, using the Maxwell RSC Tissue DNA Kit and the Maxwell RSC Instrument (Promega, Madison, WI, United States). For FFPE sample, DNA was extracted using the QIAamp DNA FFPE

(299) Conserved Role of Heterotrimeric G Proteins in Plant Defense and Cell Death ProgressionGenes (Basel)January 18, 2024Parastoo Karimian, Yuri Trusov, Jose Ramon BotellaRNA was isolated from leaves using a Maxwell® RSC Plant RNA Kit (Promega, Madison, WI, USA) with a Maxwell® RSC instrument. cDNA was synthesized using an iScript TM cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA) according

(300) New Gall-Forming Insect Model, Smicronyx madaranus: Critical Stages for Gall Formation, Phylogeny, and Effectiveness of Gene Functional AnalysisInsectsJanuary 16, 2024Ryo Ushima, Ryoma Sugimoto, Yota Sano, Hinako Ogi, Ryuichiro Ino, Hiroshi Hayakawa, Keisuke Shimada, Tsutomu TsuchidaTokyo, Japan). Eight days after dsRNA injection, total RNA was extracted from each individual using the Maxwell RSC instrument and Maxwell RSC simplyRNA Tissue Kit (Promega). Quantitative PCR and dissociation curve analysis for

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