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Automated DNA/RNA Purification Sample Preparation Workstations
Maxwell RSC Instrument
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Maxwell RSC Instrument
Maxwell RSC Instrument
Maxwell RSC Instrument from Promega
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Citations:
(802)
Figures:
(1)
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Maxwell RSC Instrument
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Description
The Maxwell RSC Instrument is a platform for automated extraction of nucleic acid from a range of sample types. Extraction methods use sample lysis and binding to paramagnetic particles followed by washing steps and elution.
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Maxwell RSC Instrument
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
AS4500
Quantity
EA
Processing Time
30 to 60 min (depending on sample type and method)
Capacity
Up to 16
Dimensions
(WxDxH) 330.2 x 345.2 x 299.7 mm (13 x 13.6 x 11.8 in)
UV Lamp
Avg. lifetime approximately 6,000 hrs
Molecular Weight
~85kDa
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Promega
2800 Woods Hollow Rd
Madison
,
WI
53711
United States
Phone
: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516
Email: custserv@promega.com
techserv@promega.com
Company Profile
Website:
http://www.promega.com
Citations (802)
(321)
Naturally occurring canine laminopathy leading to a dilated and fibrosing cardiomyopathy in the Nova Scotia Duck Tolling Retriever
Scientific Reports
November 4, 2023
Danika L. Bannasch, Danielle T. Oertle, Julia Vo, Kevin L. Batcher, Joshua A. Stern, Joanna L. Kaplan, Ronald H. L. Li, Indiana E. Madden, Matthias Christen, Tosso Leeb,
et al.
samples according to standard methods using the Maxwell RSX Whole Blood DNA kit in combination with the
Maxwell RSC instrument
(
Promega
, Dübendorf, Switzerland).
Genome-wide association study
The Illumina Canine HD BeadChip was
(322)
Food Administration and Not Genetic Variants Causes Pharmacokinetic Variability of Tadalafil and Finasteride
Journal of Personalized Medicine
October 31, 2023
Gonzalo Villapalos-García, Pablo Zubiaur, Cristina Marián-Revilla, Paula Soria-Chacartegui, Marcos Navares-Gómez, Gina Mejía-Abril, Andrea Rodríguez-Lopez, Eva González-Iglesias, Samuel Martín-Vílchez, Manuel Román,
et al.
performed using either a MagNa Pure automatic DNA extractor (Roche Applied Science, Penzberg, Germany) or a
Maxwell® RSC instrument
(
Promega
, Madison, WI, USA). DNA concentration was measured with a
Qubit 3.0 Fluorometer
(
ThermoFisher
,
(323)
Spermidine Ameliorates Colitis via Induction of Anti-Inflammatory Macrophages and Prevention of Intestinal Dysbiosis
Journal of Crohn's and Colitis
October 20, 2023
Anna Niechcial, Marlene Schwarzfischer, Marcin Wawrzyniak, Kirstin Atrott, Andrea Laimbacher, Yasser Morsy, Egle Katkeviciute, Janine Häfliger, Patrick Westermann, Cezmi A Akdis,
et al.
[PBS], and RNA isolation was performed using the
Maxwell RSC simplyRNA Tissue Kit
[
Promega
] and the
Maxwell RSC Instrument
[
Promega
] according to the manufacturer’s instructions. RNA concentration was determined by measuring
(324)
Genetic signature detected in T cell receptors from patients with severe COVID-19
iScience
October 20, 2023
Manuel Corpas, Carmen de Mendoza, Víctor Moreno-Torres, Ilduara Pintos, Pedro Seoane, James R. Perkins, Juan A.G. Ranea, Segun Fatumo, Tamas Korcsmaros, José Manuel Martín-Villa,
et al.
isolated from buffy coat frozen samples with the
Maxwell RSC buffy coat DNA kit
(
Promega
) using the
Maxwell RSC Instrument
(
Promega
), following manufacturer recommendations. After isolation, the purity of genomic DNA was analyzed
(325)
Semaphorin 3C promotes de novo steroidogenesis in prostate cancer cells
Endocrine-Related Cancer
October 6, 2023
Parvin Yenki, Satyam Bhasin, Liang Liu, Noushin Nabavi, Chi Wing Cheng, Kevin J Tam, James W Peacock, Hans H Adomat, Tabitha Tombe, Ladan Fazli,
et al.
RSC simply RNA Tissue Kit (
Promega
, Cat. No.
AS1340
) using
Maxwell RSC Instrument
(
Promega
, Cat. No.
AS4500
). Following quality control by TapeStation system, strand-specific RNA library was prepared using TruSeq
(326)
Epithelial ovarian cancer is infiltrated by activated effector T cells co-expressing CD39, PD-1, TIM-3, CD137 and interacting with cancer cells and myeloid cells
Frontiers in Immunology
October 4, 2023
Elena Tassi, Alice Bergamini, Jessica Wignall, Miriam Sant’Angelo, Emanuela Brunetto, Chiara Balestrieri, Miriam Redegalli, Alessia Potenza, Danilo Abbati, Francesco Manfredi,
et al.
calculated.
Gene expression analysis
RNA was extracted from FFPE samples by using
Maxwell RSC RNA FFPE Kit
and
Maxwell RSC Instrument
(
Promega
), according to the manufacturer’s instructions. Elution was performed in 50 μl and RNA was
(327)
The Virucidal Effect of the Chlorination of Water at the Initial Phase of Disinfection May Be Underestimated If Contact Time Calculations Are Used
Pathogens
October 3, 2023
Fredy Saguti, Inger Kjellberg, Marianela Patzi Churqui, Hao Wang, Timur Tunovic, Jakob Ottoson, Olof Bergstedt, Helene Norder, Kristina Nyström
each three
viruses
from both control and treated samples with disinfectants in
Maxwell RSC instrument
AS4500
(
Promega
, Madison, WI, USA) using
Maxwell RSC Viral Total Nucleic Acid Purification kit
(
Promega
, WI,
(328)
UV-radiation and MC1R germline mutations are risk factors for the development of conventional and spitzoid melanomas in children and adolescents
eBioMedicine
October 1, 2023
Alexandra Liebmann, Jakob Admard, Sorin Armeanu-Ebinger, Hannah Wild, Michael Abele, Axel Gschwind, Olga Seibel-Kelemen, Christian Seitz, Irina Bonzheim, Olaf Riess,
et al.
tumour and normal tissue paraffin sections using the Maxwell® RSC DNA FFPE Plus Kit (
AS1720
) and the
Maxwell® RSC Instrument
(
Promega
, Madison, WI, USA) according to the manufacturer’s instructions. Three samples had to be excluded
(329)
Genetic variants in melanogenesis proteins TYRP1 and TYR are associated with the golden rhesus macaque phenotype
G3: Genes, Genomes, Genetics
September 30, 2023
Samuel M Peterson, Marina M Watowich, Lauren M Renner, Samantha Martin, Emma Offenberg, Amanda Lea, Michael J Montague, James P Higham, Noah Snyder-Mackler, Martha Neuringer,
et al.
accessed through the ONPRC Genetic Core DNA Bank or extracted from archived tissue samples using a
Maxwell RSC Instrument
and following the manufacturer's protocol (
Promega
, Inc.). Genotyping-by-sequencing (GBS) libraries
(330)
CD8+ cells and small viral reservoirs facilitate post-ART control of SIV replication in M3+ Mauritian cynomolgus macaques initiated on ART two weeks post-infection
PLOS Pathogens
September 25, 2023
Olivia E. Harwood, Lea M. Matschke, Ryan V. Moriarty, Alexis J. Balgeman, Abigail J. Weaver, Amy L. Ellis-Connell, Andrea M. Weiler, Lee C. Winchester, Courtney V. Fletcher, Thomas C. Friedrich,
et al.
lymph node
(LN) mononuclear cells (LNMC) isolated from LN
biopsies
using the SimplyRNA Tissue kit for the
Maxwell RSC instrument
(
Promega
, Madison, WI). RNA was then tested for both SIV gag and a cellular gene, β-actin, by
RT-qPCR
.
(331)
Next-generation sequencing improves precision medicine in hearing loss
Frontiers in Genetics
September 22, 2023
T. Imizcoz, C. Prieto-Matos, R. Manrique-Huarte, D. Calavia, A. Huarte, P. C. Pruneda, G. R. Ordoñez, E. Cañada-Higueras, A. Patiño-García, G. Alkorta-Aranburu,
et al.
bp of flanking intronic regions. Briefly, DNA was isolated from peripheral blood or saliva using the
Maxwell RSC Instrument
(
Promega
) and characterized by Qubit (ThermoFisher). Target-region capture using
Agilent
SureSelect
(332)
Immunologic constant of rejection as a predictive biomarker of immune checkpoint inhibitors efficacy in non-small cell lung cancer
Journal of Translational Medicine
September 19, 2023
Alice Mogenet, Pascal Finetti, Emilie Denicolai, Laurent Greillier, Pascaline Boudou-Rouquette, François Goldwasser, Gwenael Lumet, Michele Ceccarelli, Daniel Birnbaum, Davide Bedognetti,
et al.
informed consent for the use of archived material for research purpose.
Tumor
RNA was extracted using “
Maxwell® RSC Instrument
” (
Promega
) shortly after microtome
dissection
to prevent nucleic acid degradation. After extraction,
(333)
Lung microenvironments harbor Mycobacterium tuberculosis phenotypes with distinct treatment responses
Antimicrobial Agents and Chemotherapy
September 19, 2023
Nicholas D. Walter, Jackie P. Ernest, Christian Dide-Agossou, Allison A. Bauman, Michelle E. Ramey, Karen Rossmassler, Lisa M. Massoudi, Samantha Pauly, Reem Al Mubarak, Martin I. Voskuil,
et al.
min
incubation
at 37°C after each DNase addition. RNA was further purified and DNA was digested on a
Maxwell RSC instrument
(
Promega
) with the
Maxwell RSC simplyRNA tissue kit
.
Purification
was performed following the manufacturer’s
(334)
Wastewater surveillance of Mpox virus in Baltimore
Science of The Total Environment
September 15, 2023
Samendra P. Sherchan, Tamunobelema Solomon, Oladele Idris, Daniel Nwaubani, Ocean Thakali
increase within a month,"Wannigama et al., 2023" Spain,Grab,Aluminum adsorption-precipitation method,
Maxwell® RSC Instrument
(
Promega
) using the Maxwell RSC Pure Food GMO and authentication kit (Promega),CDC G2R_G and G2R_WA
(335)
Evaluation of commercial kits for isolation and bisulfite conversion of circulating cell-free tumor DNA from blood
Clinical Epigenetics
September 14, 2023
Stine H. Kresse, Sara Brandt-Winge, Heidi Pharo, Bjørnar T. B. Flatin, Marine Jeanmougin, Hege Marie Vedeld, Guro E. Lind
cfDNA isolation was performed according to the manufacturer’s instructions. For the Maxwell kit, the
Maxwell RSC Instrument
(
Promega
) was used for automated isolation according to the manufacturer’s instructions. For the CNA
(336)
Targeting microRNA-145-mediated progressive phenotypes of early bladder cancer in a molecularly defined in vivo model
Molecular Therapy - Nucleic Acids
September 12, 2023
Kazuki Heishima, Nobuhiko Sugito, Chikara Abe, Akihiro Hirata, Hiroki Sakai, Yukihiro Akao
Maxwell RSC simplyRNA Cells Kit
(
Promega
, Madison, WI,
AS1390
) and a
Maxwell RSC instrument
(
Promega
,
AS4500
). BC cells (253 JB-V) were
transfected
and incubated for 48 h with miRNAs (miR-145-5p, 10 nM; control
(337)
Comparison of Two Variant Analysis Programs for Next-Generation Sequencing Data of Whole Mitochondrial Genome
Journal of Korean Medical Science
September 11, 2023
Seung Eun Lee, Ga Eun Kim, Hajin Kim, Doo Hyun Chung, Soong Deok Lee, Moon-Young Kim
sample, DNA was extracted using
Maxwell RSC DNA FFPE kit
(
Promega Corporation
, Madison, WI, USA) on
Maxwell RSC instrument
(
Promega Corporation
). It was quantified with
Invitrogen Qubit Flex Fluorometer
(
Thermo Fisher Scientific
),
(338)
Implementation of pharmacogenetic testing in oncology: DPYD-guided dosing to prevent fluoropyrimidine toxicity in British Columbia
Frontiers in Pharmacology
September 8, 2023
Angela Wu, Helen Anderson, Curtis Hughesman, Sean Young, Caroline Lohrisch, Colin J. D. Ross, Bruce C. Carleton
(gDNA) was extracted from 150 µL of isolated buffy coat using the
Maxwell RSC Buffy Coat DNA Kit
and
Maxwell RSC instrument
(
Promega
, Wisconsin, United States). DPYD testing was performed by
quantitative PCR
(
qPCR
) on a QuantStudio
(339)
Effects of a novel, non-invasive pre-hatch application of probiotic for broilers on development of cecum microbiota and production performance
Animal Microbiome
September 5, 2023
Kasper Rømer Villumsen, Dorthe Sandvang, Gisle Vestergård, Mia Son Räfle Olsen, Johanne Juul, Morten Dencker, Johannes Kudsk, Louise Ladefoged Poulsen
before the supernatant was transferred to a new tube and used as DNA template. II) Extracted by use of
Maxwell® RSC Instrument
(
Promega
, Denmark) using
Maxwell® RSC Cultured Cells DNA kit
(AS 1620) (
Promega
, Denmark). Subsequently
(340)
BL-MOL-AR Project, Preliminary Results about Liquid Biopsy: Molecular Approach Experience and Research Activity in Oncological Settings
Global Medical Genetics
September 1, 2023
Alessandro Pancrazzi, Francesco Bloise, Alice Moncada, Roberta Perticucci, Stefania Vecchietti, Francesca Pompili, Francesca Ricciarini, Silvia Lenzi, Cristina Gatteschi, Sabrina Giusti,
et al.
for purification
of poor plasma. 20 21 The cfDNA extraction was performed with the Maxwell RSC Extractor, code
AS4500
, using the
Maxwell RSC ccfDNA Plasma Kit
, code
AS1480
(
Promega
SRL, Milan, Italy). 22 The amount of
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Maxwell RSC Instrument from Promega
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