Maxwell RSC Instrument from Promega

Product Specs
ItemMaxwell RSC Instrument
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS4500
QuantityEA
Processing Time30 to 60 min (depending on sample type and method)
CapacityUp to 16
Dimensions(WxDxH) 330.2 x 345.2 x 299.7 mm (13 x 13.6 x 11.8 in)
UV LampAvg. lifetime approximately 6,000 hrs
Molecular Weight~85kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(321) Beneficial effect of heat-killed Lactiplantibacillus plantarum L-137 on intestinal barrier function of rat small intestinal epithelial cellsScientific ReportsMay 29, 2024Mototsugu Watanabe, Hiroko Nakai, Tatsuya Ohara, Kengo Kawasaki, Shinji Murosaki, Yoshitaka HiroseMaxwell® RSC simplyRNA Cells Kit (Promega, Madison, WI, USA) and automated RNA extraction system by using the Maxwell® RSC Instrument (Promega) according to the manufacturer’s instructions37–39. The RNA was eluted with 50 μL of nuclease-free

(322) Detection Method for Gene Doping in a Mouse Model Expressing Human Erythropoietin from Adeno-Associated Virus Vector-9Genes (Basel)May 29, 2024Takehito Sugasawa, Atsushi Hirokawa, Norihiro Otani, Yasuharu Kanki, Kieu DM Nguyen, Tohru Takemasa, Koichi Watanabe, Yoshinori Takeuchi, Naoya Yahagi, Yoichiro Takahashitotal DNA from 100 µL (short-term experiment) or 50 µL (long-term experiment) of whole blood, using a Maxwell RSC Instrument (Promega), according to the manufacturer’s instructions. The DNAs were dissolved in 50 µL of Milli-Q

(323) Field validation of an eDNA assay for nutria illuminates a role in invasive species managementEcology and EvolutionMay 23, 2024Stephanie S. Costerand subjected to centrifugation for 10 min. at 16,000 g and the supernatant was used downstream in a Maxwell RSC Instrument (Promega) with the Maxwell RSC PureFood GMO and Authentication Kit (Promega) following the recommended

(324) Capsid Integrity Detection of Enteric Viruses in Reclaimed WatersVirusesMay 21, 2024Pablo Puchades-Colera, Azahara Díaz-Reolid, Inés Girón-Guzmán, Enric Cuevas-Ferrando, Alba Pérez-Cataluña, Gloria SánchezQuantification Nucleic acid extraction from influent wastewater and reclaimed water concentrates was performed using the Maxwell® RSC Instrument (Promega, Madison, WI, USA) with the Maxwell RSC Pure Food GMO and authentication kit (Promega) and

(325) Isolation and Characterization of Cell-Free DNA from Cerebral OrganoidsInternational Journal of Molecular SciencesMay 18, 2024Brian B. Silver, Ashley Brooks, Kevin Gerrish, Erik J. TokarGene Expression Genomic DNA (gDNA) from organoids and cfDNA from conditioned media were extracted using the Promega Maxwell® RSC instrument (Promega, Madison, WI, USA) with the Tissue DNA Kit or ccfDNA Plasma Kit, respectively. DNA concentrations

(326) High-throughput surface epitope immunoaffinity isolation of extracellular vesicles and downstream analysisBiology Methods and ProtocolsMay 17, 2024Ramin Khanabdali, Michelle Mandrekar, Rick Grygiel, Phuoc-An Vo, Carlos Palma, Sara Nikseresht, Siena Barton, Mozhgan Shojaee, Sadman Bhuiyan, Kartini Asari, et al.analyses, bead-captured EVs were lysed with 1% SDS. SEI isolation of EVs was also implemented on the Maxwell RSC instrument (Promega) for medium-throughput sample processing (see Supplementary Fig. S1). EV isolation method

(327) Closing the gap: Oxford Nanopore Technologies R10 sequencing allows comparable results to Illumina sequencing for SNP-based outbreak investigation of bacterial pathogensJournal of Clinical MicrobiologyMay 8, 2024Bert Bogaerts, An Van den Bossche, Bavo Verhaegen, Laurence Delbrassinne, Wesley Mattheus, Stéphanie Nouws, Maxime Godfroid, Stefan Hoffman, Nancy H. C. Roosens, Sigrid C. J. De Keersmaecker, et al.described elsewhere (24, 25). The DNA extraction of the E. coli ONT data sets was performed using the Maxwell RSC Instrument (Promega, WI, USA) according to the manufacturer’s instructions. For the Illumina and ONT sequencing

(328) The stress-responsive cytotoxic effect of diesel exhaust particles on lymphatic endothelial cellsScientific ReportsMay 7, 2024Yu Sakurai, Eiki Oba, Akiko Honda, Hiroki Tanaka, Hirohisa Takano, Hidetaka AkitaPBS, and then total RNA was extracted from the cells using the Maxwell RSC simplyRNA Tissue Kit and Maxwell RSC Instrument (Promega, Madison, WI, U.S.A). The extracted RNAs from 3 samples individually treated with each concentration

(329) An Evaluation of High-Risk HPV in Squamous Cell Carcinomas of the Lip in a South African CohortHead and Neck PathologyMay 6, 2024Sharon N. Harbor, Johann W. Schneider, Nadine Solomons, Micheline Sanderson, Amir H. AfroghehTotal RNA was extracted from samples using the Maxwell® RSC RNA FFPE kit (Promega, Wisconsin, USA) and Maxwell RSC instrument (Promega, Wisconsin, USA), following the manufacturer’s instructions. RNA quality was assessed using

(330) Acute Lymphoblastic Leukemia with Myeloid Mutations Is a High-Risk Disease Associated with Clonal HematopoiesisBlood Cancer DiscoveryMay 1, 2024Caner Saygin, Pu Zhang, Jacob Stauber, Ibrahim Aldoss, Adam S. Sperling, Lachelle D. Weeks, Marlise R. Luskin, Todd C. Knepper, Pankhuri Wanjari, Peng Wang, et al.extracted from peripheral blood or bone marrow collected in EDTA tubes using the simplyRNA Blood Kit on Maxwell RSC instrument (Promega) and quantified using a Qubit fluorometric assay (Thermo Fisher Scientific), adjusted for the

(331) Role of CES1 and ABCB1 Genetic Polymorphisms on Functional Response to Dabigatran in Patients with Atrial FibrillationJournal of Clinical MedicineApril 26, 2024Luca Cumitini, Giulia Renda, Mara Giordano, Roberta Rolla, Tarek Shail, Sara Sacchetti, Lorena Iezzi, Luca Giacomini, Valentina Zanotti, Raffaella Auciello, et al.extracted <1 month after collection. DNA extraction was performed using the Maxwell Whole Blood DNA kit on Maxwell® RSC instrument (Promega, Hollow Road Madison, WI, USA). After 5 min at room temperature, approximately 400 μL of each

(332) A MiR181/Sirtuin1 regulatory circuit modulates drug response in biliary cancersClinical and experimental medicineApril 10, 2024Barbato A, Piscopo F, Salati M, Pollastro C, Evangelista L, Ferrante L, Limongello D, Brillante S, Iuliano A, Reggiani-Bonetti L, et al.Maxwell RSC simplyRNA Cells (Glomax Discover System, Promega, Madison, WI, USA) and processed with Maxwell RSC Instrument (Glomax Discover System, Promega, Madison, WI, USA) following manufacturer’s instructions [21]. Single-stranded

(333) IKZF1plus is a frequent biomarker of adverse prognosis in Mexican pediatric patients with B-acute lymphoblastic leukemiaFrontiers in OncologyApril 3, 2024Joaquin Garcia-Solorio, Juan Carlos Núñez-Enriquez, Marco Jiménez-Olivares, Janet Flores-Lujano, Fernanda Flores-Espino, Carolina Molina-Garay, Alejandra Cervera, Diana Casique-Aguirre, José Gabriel Peñaloza-Gonzalez, Ma. Del Rocío Baños-Lara, et al.patients was 1.4 years. 2.2 Nucleic acid extraction and quantification DNA was isolated using the Maxwell® RSC Instrument (Promega Corporation, Madison, WI, USA). DNA concentration and quality were evaluated using a NanoDrop® 2000 spectrophotometer

(334) Intestinal permeability, gut inflammation, and gut immune system response are linked to aging-related changes in gut microbiota composition: a study in female miceThe Journals of Gerontology, Series A: Biological Sciences and Medical SciencesApril 1, 2024Paola Elizabeth González-Muniesa, , Paola Elizabeth Félix-Soriano, Mirian Félix-Soriano, Mirian Samblas, María Jesús Moreno-Aliaga, , María Jesús González-Muniesaestablished protocol. Briefly, the Maxwell RSC Fecal Microbiome DNA kit (Promega, Madison, WI, USA) in the Maxwell RSC Instrument was used to efficiently isolate DNA from OMNIgene. GUT kits following the manufacturer’s instructions;

(335) Chemi-Northern: a versatile chemiluminescent northern blot method for analysis and quantitation of RNA moleculesRNAApril 1, 2024Katherine M. McKenney, Robert P. Connacher, Elise B. Dunshee, Aaron C. Goldstrohmmin. RNA was purified from the cell pellet using the Maxwell simplyRNA Cells Kit (Promega, AS1390) and Maxwell RSC instrument, following the manufacturer's protocol. For the on-bead DNase digestion, the amount of DNase I (Promega,

(336) Gut Microbiome and Metabolic and Immune Indices in Males with or without Evidence of Metabolic DysregulationJournal of Obesity & Metabolic SyndromeMarch 30, 2024Kyle M. Hatton-Jones, Nicholas P. West, Mike W.C. Thang, Pin-Yen Chen, Peter Davoren, Allan W. Cripps, Amanda J. Coxstored in PAXgene Blood RNA (BD Biosciences) tubes was thawed and RNA was isolated using the automated Maxwell RSC Instrument (Promega) and the RSC simplyRNA tissue kit (Promega). Isolated RNA was analysed using both the commercially

(337) Comparative Analyses of Targeted Myeloid Cancer Next-Generation Sequencing Panel in Fresh Blood, Bone Marrow and FFPE MaterialInt J Mol SciMarch 21, 2024Andrea Daniela Hobeck, Sophia Wendt, Saskia Krohn, Gudrun Knuebel, Stephan Bartels, Elisa Schipper, Christian Junghanss, Hugo Murua EscobarHannover Medical School, Germany. FFPE samples were prepared with the Maxwell© RSC DNA FFPE kit on a Maxwell© RSC instrument (Promega, Fitchburg, MA, USA) for gDNA isolation, and quantification was performed with a Qubit™ dsDNA HS Assay Kit

(338) A single workflow for multi-species blood transcriptomicsBMC GenomicsMarch 16, 2024Elody Orcel, Hayat Hage, May Taha, Noémie Boucher, Emilie Chautard, Virginie Courtois, Adrien Saliouthe RNA extraction and the library preparation. Our extraction protocol is already integrated on the Maxwell RSC Instrument (Promega). Conclusions We recommend the following workflow for blood transcriptomics analysis: (i)

(339) Betanin Attenuates Epigenetic Mechanisms and UV-Induced DNA Fragmentation in HaCaT Cells: Implications for Skin Cancer ChemopreventionNutrientsMarch 16, 2024Afshin Zand, Sodbuyan Enkhbilguun, John M. Macharia, Krisztina Varajti, Istvan Szabó, Gellért Gerencsér, Boglárka Bernadett Tisza, Bence L. Raposa, Zoltán Gyöngyi, Timea VarjasQuantitative Real-Time PCR Analysis Total RNA was isolated via a semiautomatic workflow with the help of the Maxwell® RSC Instrument (Promega, Fitchburg, WI, USA) using a Maxwell® RSC RNA FFPE Kit (AS1440, Promega, Fitchburg, WI, USA).

(340) First Detection of Benign Rabbit Caliciviruses in ChileVirusesMarch 12, 2024Elena Smertina, Luca M. Keller, Nina Huang, Gabriela Flores-Benner, Jennifer Paola Correa-Cuadros, Melanie Duclos, Fabian M. Jaksic, Cristóbal Briceño, Victor Neira Ramirez, Miguel Díaz-Gacitúa, et al.homogeniser (Bertin Technologies, Montigny-le-Bretonneux, France). Total RNA was extracted using the Maxwell® RSC instrument (Promega, Madison, WI, USA) in combination with the Maxwell® RSC SimplyRNA Tissue Kit (Promega, Madison,

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