Maxwell RSC Instrument from Promega

Product Specs
ItemMaxwell RSC Instrument
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS4500
QuantityEA
Processing Time30 to 60 min (depending on sample type and method)
CapacityUp to 16
Dimensions(WxDxH) 330.2 x 345.2 x 299.7 mm (13 x 13.6 x 11.8 in)
UV LampAvg. lifetime approximately 6,000 hrs
Molecular Weight~85kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(181) Targeted C-to-T Base Editing in the Arabidopsis Plastid GenomeCurr ProtocJanuary 6, 2025Issei Nakazato, Shin-Ichi Arimuracat. no. MT020‐01H)Zirconia Ball φ5mm (NIKKATO, cat. no. 5‐4060‐33)Multi‐beads Shocker (Yasui Kikai)Maxwell® RSC Instrument (Promega) with compatible computer Additional reagents and equipment for aseptically sowing seeds (see

(182) Whole genome sequencing analysis of enteropathogenic Escherichia coli from human and companion animals in KoreaJournal of Veterinary ScienceJanuary 1, 2025Jae Young Oh, Kyung-Hyo Do, Jae Hong Jeong, SuMin Kwak, Sujin Choe, Dongheui An, Jong-Chan Chae, Kwangjun Lee, Kwang-Won Seohttp://pubmlst.org/biqsdb?db=pubmlst_ecoli_achtman_seqdef. Whole-genome sequencing Genomic DNA from each strain was extracted from overnight cultures using a Maxwell RSC Instrument (Promega, USA), following the manufacturer’s instructions. DNA concentration was determined using a

(183) Buccal DNA global methylation and cognitive performance in stunted children under five years of ageThe Journal of Biomedical ResearchJanuary 1, 2025Ahmad Rusdan Handoyo Utomo, Yusnita Yusnita, Siti Maulidya Sari, Octaviani Indrasari Ranakusuma, Sunu Bagaskara, Wening Sari, Yulia Suciati, Anggi Puspa Nur Hidayati, Silviatun Nihayah, Catur Anggono Putro, et al.(Maxwell® RSC Blood DNA Kit, Cat. AS1400, Promega, Madison, WI, USA) with magnetic bead technology (Maxwell® RSC Instrument, Promega). The DNA concentration was quantified using spectrophotometry (Tecan Infinite M200 Pro, Switzerland). Epigenetic

(184) Mitochondrial trifunctional protein deficiency caused by a deep intronic deletion leading to aberrant splicingJIMD ReportsJanuary 1, 2025Thomas Cassini, Sarah Silverstein, Molly Behan, Cynthia J. Tifft, May Christine Malicdan, David R. Adams, Undiagnosed Diseases Network, Sun‐Young Ahn, Debra S. Regier(A1285901, Gibco/Thermofisher), washed with PBS and pelleted. Total RNA was extracted using Maxwell® RSC (AS4500, Promega) and Maxwell® RSC simplyRNA Cells Kit (AS1390, Promega) following manufacturer's instructions.

(185) Overexpression of CCL-20 and CXCL-8 genes enhances tumor escape and resistance to cemiplimab, a programmed cell death protein-1 (PD-1) inhibitor, in patients with locally advanced and metastatic cutaneous squamous cell carcinomaOncoImmunologyDecember 31, 2024Vincenzo De Falco, Stefania Napolitano, Renato Franco, Federica Zito Marino, Luigi Formisano, Daniela Esposito, Gabriella Suarato, Rossella Napolitano, Alfonso Esposito, Francesco Caraglia, et al.tumor samples and 3 healthy samples was extracted using Maxwell® RSC RNA FFPE Kit (Promega) by using Maxwell RSC Instrument of Promega Corporation according to manufacturers’ standard protocol. Total RNA quantification and quality

(186) The genome of the sapphire damselfish Chrysiptera cyanea: a new resource to support further investigation of the evolution of PomacentridsGigabyteDecember 31, 2024Emma Gairin, Saori Miura, Hiroki Takamiyagi, Marcela Herrera, Vincent Laudetgill, liver, muscle, and brain tissue using the Maxwell® RSC simplyRNA tissue kits and an automated Maxwell® RSC instrument, following manufacturer recommendations (Promega, Cat. No. AS2000, Wisconsin, USA). The quality and

(187) Effect of Genetic Variants on Rosuvastatin Pharmacokinetics in Healthy Volunteers: Involvement of ABCG2, SLCO1B1 and NAT2International Journal of Molecular SciencesDecember 30, 2024Eva González-Iglesias, Clara Méndez-Ponce, Dolores Ochoa, Manuel Román, Gina Mejía-Abril, Samuel Martín-Vilchez, Alejandro de Miguel, Antía Gómez-Fernández, Andrea Rodríguez-Lopez, Paula Soria-Chacartegui, et al.reference formulations. 4.4. Genotyping and Phenotyping DNA was extracted from peripheral blood using a Maxwell® RSC instrument (Promega, Madison, WI, USA). DNA concentration was measured using a Qubit 3.0 fluorometer (ThermoFisher,

(188) Distinct microbes, metabolites, and the host genome define the multi-omics profiles in right-sided and left-sided colon cancerMicrobiomeDecember 28, 2024Lei Liang, Cheng Kong, Jinming Li, Guang Liu, Jinwang Wei, Guan Wang, Qinying Wang, Yongzhi Yang, Debing Shi, Xinxiang Li, et al.a Maxwell RSC Blood DNA Kit (cat# AS1400, Promega, Madison, WI, USA) on a Maxwell RSC system (cat# AS4500, Promega). For formalin-fixed, paraffin-embedded (FFPE) tissue, DNA was extracted using a MagMAX FFPE DNA/RNA Ultra Kit

(189) Air pollution exposure is associated with gene expression in childrenEnvironmental EpigeneticsDecember 24, 2024Siddhartha Das, Amanda Rundblad, Irene Fontes Marques, Ana Goncalves Soares, Vincent W Jaddoe, Martine Vrijheid, Mark Nieuwenhuijsen, Joost Verlouw, Jason Matthews, Kirsten B Holven, et al.before RNA isolation. The Maxwell® RSC miRNA Tissue kits and Maxwell® RSC instrument (artnr. AS1460 and AS4500, respectively; Promega, Madison, WI, USA) were used for extraction of total RNA, including microRNA.

(190) Maternal genomic profile, gestational diabetes control, and Mediterranean diet to prevent low birth weightiScienceDecember 20, 2024Ana M. Ramos-Levi, Rocío Martín O'Connor, Ana Barabash, Maria Paz de Miguel, Angel Diaz-Perez, Clara Marcuello, Cristina Familiar, Inmaculada Moraga, Maria Arnoriaga-Rodriguez, Johanna Valerio, et al.overnight fast, at the time of the OGTT for screening of GDM, between 12 and 14 GW (Visit 1), using the Maxwell RSC instrument (Promega, Dubendorf, Switzerland). Genotyping was performed by IPLEX MassARRAY PCR using the Agena platform

(191) Proof of Concept Study: Comparison of Semi-Automated RNA Isolation Methods from Archived Formalin-Fixed, Paraffin-Embedded Tissues with Clinical Routine RNA Isolation MethodsMethods and ProtocolsDecember 19, 2024Patrick Hannibal Dalsbo Petersen, Jaslin Pallikkunnath James, Lene Buhl Riis, Claus Kim Høgdall, Estrid Vilma HøgdallµL of mineral oil using the Maxwell® RSC RNA FFPE Kit (Promega, Madison, WI, USA, Cat: AS1440) on a Maxwell® RSC Instrument (Promega) according to the manufacturer’s protocol [16]. The RNA was eluted in 55 µL of Tris-EDTA buffer. Roche

(192) Clinical exome next‑generation sequencing panel for hereditary pheochromocytoma and paraganglioma diagnosisExperimental and Therapeutic MedicineDecember 18, 2024Beatrice Melli, Vincenza Ylenia Cusenza, Sandra Martinelli, Federica Castiglione, Loretta Fornaciari, Andrea Palicelli, Luca Braglia, Enrico Farnetti, Aurelio Negro, Simonetta Rosato, et al.Maxwell® 16 LEV Blood DNA Kit (AS1290; Promega Corporation) according to the manufacturer's protocol on the Maxwell® RSC instrument (Promega Corporation) according to the manufacturer's specifications. The TruSight One clinical exome

(193) Effect of elite sport activity on salivary microbiota: The case of water poloHeliyonDecember 15, 2024Iolanda Veneruso, Cristina Mennitti, Alessandro Gentile, Gennaro Di Bonito, Jacopo Ulisse, Carmela Scarano, Barbara Lombardo, Daniela Terracciano, Raffaela Pero, Giovanni D'Alicandro, et al.all the collected and pelleted saliva samples by using the Maxwell® RSC Buccal Swab DNA Kit and the Maxwell RSC instrument (both from Promega, Madison, WI, USA). In particular, 200 μl of pelleted saliva samples were transferred

(194) Patients with a Wide Range of Disorders Related to WFS1 Gene Variants: Novel Mutations and Genotype–Phenotype CorrelationsGenes (Basel)December 12, 2024Julia Grzybowska-Adamowicz, Karolina Gadzalska, Paulina Jakiel, Ewa Juścińska, Monika Gorządek, Sebastian Skoczylas, Tomasz Płoszaj, Przemysława Jarosz-Chobot, Irina Kowalska, Małgorzata Myśliwiec, et al.procedures. 2.2. Sequencing 2.2.1. Library Preparation DNA was extracted from peripheral blood using a Maxwell® RSC Instrument (Maxwell® RSC Blood DNA Kit, Promega, Madison, WI, USA). The library was prepared using the Agilent

(195) In-House Validation of Four Duplex Droplet Digital PCR Assays to Quantify GM Soybean EventsFoodsDecember 11, 2024Daniela Verginelli, Sara Ciuffa, Katia Spinella, Davide La Rocca, Marisa Misto, Cinzia Quarchioni, Pamela Bonini, Cristiana Fusco, Lorella Peroni, Stefania Peddis, et al.Purity DNA was extracted from 150 mg of CRM powder by using the RSC PureFood GMO kit and the extractor Maxwell®RSC Instrument (Promega Madison, WI, USA), according to the manufacturer’s instructions. The concentration and the

(196) Metabolic, genetic and immunological features of relatives of type 1 diabetes patients with elevated insulin resistanceJournal of Endocrinological InvestigationDecember 10, 2024V. Codazzi, V. Salvatore, F. Ragogna, I. Marzinotto, A. Anselmo, N. Baldoni, M. R. Pastore, S. Martinenghi, A. Stabilini, E. Bosi, et al.pg/mL. DNA extraction and T1D genetic risk score 1 DNA was extracted from whole blood samples using the Maxwell® RSC Instrument and the Maxwell® RSC Blood DNA Kit by Promega (cat #AS1400). The T1D genetic risk score 1 (GRS1), based

(197) Comparative Analysis of Gut Microbiota in Humans Living with and Without Companion AnimalsLife (Basel)December 6, 2024Kyung-Hyo Do, Jiwon Park, Nahee Kim, Dahye Ryu, Min-Gyu Kim, Hyunjung Ahn, Hakhyun Kim, Jun-Gi Hwang, Min-Kyu Park, Kwang-Won Seo, et al.−70 °C. From each individual’s fecal sample, 200 mg was used to extract bacterial DNA utilizing the Maxwell RSC Instrument (Promega, Madison, WI, USA) and the Maxwell RSC Fecal Microbiome DNA Kit AS1700 (Promega) following

(198) Bioactivated Glucoraphanin Improves Cell Survival, Upregulating Phospho-AKT, and Modulates Genes Involved in DNA Repair in an In Vitro Alzheimer’s Disease Model: A Network-Transcriptomic AnalysisNutrientsDecember 5, 2024Aurelio Minuti, Emanuela Mazzon, Renato Iori, Luigi Chiricosta, Osvaldo Artimagnellaextracted using the Maxwell® RSC simplyRNA Cells Kit (#AS1390, Promega, Madison, WI, USA) with the Maxwell® RSC instrument according to the instructions. Afterwards, library preparation (from 100 ng of total RNA) of two biological

(199) Detection of QTLs regulating the second internode length in rice dwarf mutant d1Breeding ScienceDecember 3, 2024Quynh T. Ha, Sandar Moe, Vincent Pamugas Reyes, Kazuyuki Doi, Kotaro Miura, Mio Mizushima, Akiteru Maeno, Katsutoshi Tsuda, Keisuke Nagai, Motoyuki Ashikarilines were sampled and frozen in liquid nitrogen for RNA extraction. Total RNA was extracted using a Maxwell RSC Instrument (Promega), and first-strand cDNA was synthesized with the Omniscript RT Kit (Qiagen) and oligo(dT) 20

(200) Generation of Replication‐Competent Hepatitis B Virus Harboring Tagged Polymerase for Visualization and Quantification of the InfectionMicrobiology and ImmunologyDecember 2, 2024Chiharu Morita, Masami Wada, Eriko Ohsaki, Shihoko Kimura‐Ohba, Keiji Uedasubjected to viral DNA extraction using Maxwell RSC Viral Total Nucleic Acid Purification Kit (Promega) and Maxwell RSC Instrument (Promega) according to the manufacturer's instruction. For the preparation of HBV DNA from CsCl fractions,

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