Maxwell RSC Instrument from Promega

Product Specs
ItemMaxwell RSC Instrument
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS4500
QuantityEA
Processing Time30 to 60 min (depending on sample type and method)
CapacityUp to 16
Dimensions(WxDxH) 330.2 x 345.2 x 299.7 mm (13 x 13.6 x 11.8 in)
UV LampAvg. lifetime approximately 6,000 hrs
Molecular Weight~85kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(241) Maternal genomic profile, gestational diabetes control, and Mediterranean diet to prevent low birth weightiScienceDecember 20, 2024Ana M. Ramos-Levi, Rocío Martín O'Connor, Ana Barabash, Maria Paz de Miguel, Angel Diaz-Perez, Clara Marcuello, Cristina Familiar, Inmaculada Moraga, Maria Arnoriaga-Rodriguez, Johanna Valerio, et al.overnight fast, at the time of the OGTT for screening of GDM, between 12 and 14 GW (Visit 1), using the Maxwell RSC instrument (Promega, Dubendorf, Switzerland). Genotyping was performed by IPLEX MassARRAY PCR using the Agena platform

(242) Proof of Concept Study: Comparison of Semi-Automated RNA Isolation Methods from Archived Formalin-Fixed, Paraffin-Embedded Tissues with Clinical Routine RNA Isolation MethodsMethods and ProtocolsDecember 19, 2024Patrick Hannibal Dalsbo Petersen, Jaslin Pallikkunnath James, Lene Buhl Riis, Claus Kim Høgdall, Estrid Vilma HøgdallµL of mineral oil using the Maxwell® RSC RNA FFPE Kit (Promega, Madison, WI, USA, Cat: AS1440) on a Maxwell® RSC Instrument (Promega) according to the manufacturer’s protocol [16]. The RNA was eluted in 55 µL of Tris-EDTA buffer. Roche

(243) Clinical exome next‑generation sequencing panel for hereditary pheochromocytoma and paraganglioma diagnosisExperimental and Therapeutic MedicineDecember 18, 2024Beatrice Melli, Vincenza Ylenia Cusenza, Sandra Martinelli, Federica Castiglione, Loretta Fornaciari, Andrea Palicelli, Luca Braglia, Enrico Farnetti, Aurelio Negro, Simonetta Rosato, et al.Maxwell® 16 LEV Blood DNA Kit (AS1290; Promega Corporation) according to the manufacturer's protocol on the Maxwell® RSC instrument (Promega Corporation) according to the manufacturer's specifications. The TruSight One clinical exome

(244) Effect of elite sport activity on salivary microbiota: The case of water poloHeliyonDecember 15, 2024Iolanda Veneruso, Cristina Mennitti, Alessandro Gentile, Gennaro Di Bonito, Jacopo Ulisse, Carmela Scarano, Barbara Lombardo, Daniela Terracciano, Raffaela Pero, Giovanni D'Alicandro, et al.all the collected and pelleted saliva samples by using the Maxwell® RSC Buccal Swab DNA Kit and the Maxwell RSC instrument (both from Promega, Madison, WI, USA). In particular, 200 μl of pelleted saliva samples were transferred

(245) Patients with a Wide Range of Disorders Related to WFS1 Gene Variants: Novel Mutations and Genotype–Phenotype CorrelationsGenes (Basel)December 12, 2024Julia Grzybowska-Adamowicz, Karolina Gadzalska, Paulina Jakiel, Ewa Juścińska, Monika Gorządek, Sebastian Skoczylas, Tomasz Płoszaj, Przemysława Jarosz-Chobot, Irina Kowalska, Małgorzata Myśliwiec, et al.procedures. 2.2. Sequencing 2.2.1. Library Preparation DNA was extracted from peripheral blood using a Maxwell® RSC Instrument (Maxwell® RSC Blood DNA Kit, Promega, Madison, WI, USA). The library was prepared using the Agilent

(246) In-House Validation of Four Duplex Droplet Digital PCR Assays to Quantify GM Soybean EventsFoodsDecember 11, 2024Daniela Verginelli, Sara Ciuffa, Katia Spinella, Davide La Rocca, Marisa Misto, Cinzia Quarchioni, Pamela Bonini, Cristiana Fusco, Lorella Peroni, Stefania Peddis, et al.Purity DNA was extracted from 150 mg of CRM powder by using the RSC PureFood GMO kit and the extractor Maxwell®RSC Instrument (Promega Madison, WI, USA), according to the manufacturer’s instructions. The concentration and the

(247) Metabolic, genetic and immunological features of relatives of type 1 diabetes patients with elevated insulin resistanceJournal of Endocrinological InvestigationDecember 10, 2024V. Codazzi, V. Salvatore, F. Ragogna, I. Marzinotto, A. Anselmo, N. Baldoni, M. R. Pastore, S. Martinenghi, A. Stabilini, E. Bosi, et al.pg/mL. DNA extraction and T1D genetic risk score 1 DNA was extracted from whole blood samples using the Maxwell® RSC Instrument and the Maxwell® RSC Blood DNA Kit by Promega (cat #AS1400). The T1D genetic risk score 1 (GRS1), based

(248) Comparative Analysis of Gut Microbiota in Humans Living with and Without Companion AnimalsLife (Basel)December 6, 2024Kyung-Hyo Do, Jiwon Park, Nahee Kim, Dahye Ryu, Min-Gyu Kim, Hyunjung Ahn, Hakhyun Kim, Jun-Gi Hwang, Min-Kyu Park, Kwang-Won Seo, et al.−70 °C. From each individual’s fecal sample, 200 mg was used to extract bacterial DNA utilizing the Maxwell RSC Instrument (Promega, Madison, WI, USA) and the Maxwell RSC Fecal Microbiome DNA Kit AS1700 (Promega) following

(249) Bioactivated Glucoraphanin Improves Cell Survival, Upregulating Phospho-AKT, and Modulates Genes Involved in DNA Repair in an In Vitro Alzheimer’s Disease Model: A Network-Transcriptomic AnalysisNutrientsDecember 5, 2024Aurelio Minuti, Emanuela Mazzon, Renato Iori, Luigi Chiricosta, Osvaldo Artimagnellaextracted using the Maxwell® RSC simplyRNA Cells Kit (#AS1390, Promega, Madison, WI, USA) with the Maxwell® RSC instrument according to the instructions. Afterwards, library preparation (from 100 ng of total RNA) of two biological

(250) Detection of QTLs regulating the second internode length in rice dwarf mutant d1Breeding ScienceDecember 3, 2024Quynh T. Ha, Sandar Moe, Vincent Pamugas Reyes, Kazuyuki Doi, Kotaro Miura, Mio Mizushima, Akiteru Maeno, Katsutoshi Tsuda, Keisuke Nagai, Motoyuki Ashikarilines were sampled and frozen in liquid nitrogen for RNA extraction. Total RNA was extracted using a Maxwell RSC Instrument (Promega), and first-strand cDNA was synthesized with the Omniscript RT Kit (Qiagen) and oligo(dT) 20

(251) Generation of Replication‐Competent Hepatitis B Virus Harboring Tagged Polymerase for Visualization and Quantification of the InfectionMicrobiology and ImmunologyDecember 2, 2024Chiharu Morita, Masami Wada, Eriko Ohsaki, Shihoko Kimura‐Ohba, Keiji Uedasubjected to viral DNA extraction using Maxwell RSC Viral Total Nucleic Acid Purification Kit (Promega) and Maxwell RSC Instrument (Promega) according to the manufacturer's instruction. For the preparation of HBV DNA from CsCl fractions,

(252) Comparative assessment of three commercial kits and in house optimized PCR assays for GMO screening in food and feedMethodsXDecember 1, 2024Daniela Verginelli, Cinzia Quarchioni, Katia Spinella, Davide La Rocca, Pamela Bonini, Cristiana Fusco, Marisa Misto, Stefania Peddis, Lorella Peroni, Ugo Marchesimethod with minor modifications on buffer volume and, RSC PureFood GMO performed DNA extraction by using Maxwell®RSC Instrument (Promega Madison, WI, USA) as nucleic acid purification platform, according to the manufacturer's instructions.

(253) Discovery of Novel TULP4/ACTN4/EWSR1/ACTB::MYB and ESRRG::DNM3 Fusions Expands Molecular Landscape of Adenoid Cystic Carcinoma Beyond Fusions Between MYB/MYBL1 and NFIB GenesAmerican Journal of Surgical PathologyDecember 1, 2024Alena Skálová, Natálie Klubíčková, Martina Bradová, Abbas Agaimy, Niels J. Rupp, Ivan Damjanov, Georgina Kolnikova, Petr Martínek, Petr Šteiner, Petr Grossmann, et al.TruSight Oncology 500 Kit (Illumina). RNA was extracted using the Maxwell RSC DNA FFPE Kit and the Maxwell RSC Instrument (Promega) according to the manufacturer’s instructions and quantified using the Qubit HS RNA Assay Kit

(254) Viral coexistence and insertional mutations in the ORF8 region of SARS-CoV-2: A possible mechanism of nucleotide insertionVirus researchDecember 1, 2024Kurose M, Yamamoto A, Elsayed AMA, Lawal-Ayinde BM, Nomura T, Higashiura A, Irie T, Fukushi M, Kanda M, Tahara H, et al.analysis, viral RNA was purified from swab samples or viruses amplified in VeroE6/TMPRSS2 cells by using Maxwell RSC Instrument (Promega, Milwaukee, WI, USA). cDNA preparation and amplification were performed following protocols

(255) DNA Methylation Panels for the Differentiation of Lung and Gastric Adenocarcinomas from Other Common Primary AdenocarcinomasCancers (Basel)November 29, 2024Tina Draškovič, Lara Omahen, Maja Jerše, Nina Zidar, Nina HauptmanBisulfite Conversion Total DNA was extracted using the automated Maxwell® RSC FFPE Plus DNA Kit with the Maxwell® RSC Instrument (Promega Corporation, Madison, WI, USA). Isolation followed the manufacturer’s instructions, utilizing

(256) Upregulation of ABLIM1 Differentiates Intrahepatic Cholangiocarcinoma from Hepatocellular Carcinoma and Both Colorectal and Pancreatic Adenocarcinoma Liver MetastasesGenes (Basel)November 28, 2024Tina Draškovič, Branislava Ranković, Nina Zidar, Nina HauptmanFFPE tissue blocks. RNA isolation from the punches was performed using the customized protocol on the Maxwell® RSC Instrument (Promega Corporation, Madison, WI, USA). The protocol combined the Maxwell RSC FFPE Plus RNA Kit (Promega

(257) Human VCP mutant ALS/FTD microglia display immune and lysosomal phenotypes independently of GPNMBMolecular NeurodegenerationNovember 26, 2024Benjamin E. Clarke, Oliver J. Ziff, Giulia Tyzack, Marija Petrić Petrić Howe, Yiran Wang, Pierre Klein, Claudia A. Smith, Cameron A. Hall, Adel Helmy, Michael Howell, et al.Promega Maxwell RSC simplyRNA cells kit including DNase treatment was used for RNA extractions using the Maxwell RSC instrument. A nanodrop was used to assess RNA concentration and the 260/280 ratio. RNA integrity was assessed using

(258) Analytical Validation and Performance Evaluation of Amplicon-Based Next-Generation Sequencing Assays for Detecting ERBB2 and Other Gene Amplifications in Solid TumorsCancers (Basel)November 23, 2024Ekaterina Olkhov-Mitsel, Danny Chan, Kenneth J. Craddock, August Lin, Grace Luk, Rashmi S. Goswami, Hong Wang, Anna Plotkin, Sharon Nofech-Mozes, David M. Hwang, et al.(gDNA) was extracted from archival FFPE tumor samples (10 slides, 5 micron each) using the Promega Maxwell RSC Instrument (Promega, Madison, WI, USA). The extracted gDNA was quantified via Qubit 3 Fluorometer using Qubit dsDNA

(259) Isolation and Detection of the Emerging Pathogen Escherichia albertii in Clinical Stool Samples and the Potential Transmission by Meat Samples in RetailMicroorganismsNovember 23, 2024Muhammad Zeeshan Zafar, Klara De Rauw, Anne-Marie Van den Abeele, Marie Joossens, Lore Heyvaert, Kurt HoufMaxwell RSC Cultured Cells DNA kit (AS1620, Promega, Madison, WI, USA) on the Maxwell RSC instrument (AS4500, Promega, USA). Paired-end 2 × 150 bp sequencing was performed using the Illumina Novaseq X plus platform

(260) Nanopore Sequencing Allows Recovery of High-Quality Completely Closed Genomes of All Cronobacter Species from Powdered Infant Formula Overnight EnrichmentsMicroorganismsNovember 22, 2024Narjol Gonzalez-Escalona, Hee Jin Kwon, Yi ChenDNA from each PIF inoculated sample was extracted using the Maxwell RSC Cultured Cells DNA kit with a Maxwell RSC Instrument (Promega Corporation, Madison, WI, USA) according to the manufacturer’s instructions for Gram-negative bacteria

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