Maxwell RSC Instrument from Promega

Product Specs
ItemMaxwell RSC Instrument
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS4500
QuantityEA
Processing Time30 to 60 min (depending on sample type and method)
CapacityUp to 16
Dimensions(WxDxH) 330.2 x 345.2 x 299.7 mm (13 x 13.6 x 11.8 in)
UV LampAvg. lifetime approximately 6,000 hrs
Molecular Weight~85kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(221) A Novel Chimeric Oncolytic Virus Mediates a Multifaceted Cellular Immune Response in a Syngeneic B16 Melanoma ModelCancers (Basel)October 6, 2024Sonja Glauß, Victoria Neumeyer, Lorenz Hanesch, Janina Marek, Nina Hartmann, Gabriela M. Wiedemann, Jennifer AltomonteFrance). RNA was isolated using a Maxwell RSC SimplyRNA tissue kit (Promega, Madison, WI, USA) and the Maxwell RSC instrument (Promega). GoTaq 1-step RT-qPCR Kit (Promega) and a 7500 Real Time PCR system (Applied Biosystems, Foster

(222) Targeted phage hunting to specific Klebsiella pneumoniae clinical isolates is an efficient antibiotic resistance and infection control strategyMicrobiology SpectrumOctober 3, 2024Celia Ferriol-González, Robby Concha-Eloko, Mireia Bernabéu-Gimeno, Felipe Fernández-Cuenca, Javier E. Cañada-García, Silvia García-Cobos, Rafael Sanjuán, Pilar Domingo-CalapClean & Concentrator 5-Kit (Zymo) or Maxwell PureFood GMO and Authentication Kit (Promega) with the Maxwell RSC Instrument (Promega). Phage sequencing and assembly Sequencing libraries were prepared using the Illumina Nextera

(223) Conserved allomorphs of MR1 drive the specificity of MR1-restricted TCRsFrontiers in OncologyOctober 3, 2024Terri V. Cornforth, Nathifa Moyo, Suzanne Cole, Emily P. S. Lam, Tatiana Lobry, Ron Wolchinsky, Angharad Lloyd, Katarzyna Ward, Eleanor M. Denham, Giulia Masi, et al.(Psi) and a reference gene (RPP30) measured from the same sample. Genomic DNA was extracted using a Maxwell RSC Instrument (Promega, USA) following the manufacturer’s protocol. Extracted DNA was digested using an EcoRI HF Restriction

(224) Aberrant p53 immunostaining patterns in breast carcinoma of no special type strongly correlate with presence and type of TP53 mutationsVirchows ArchivOctober 1, 2024Hannes Armbruster, Tilman Schotte, Isabell Götting, Mathis Overkamp, Massimo Granai, Lea Louise Volmer, Veronika Bahlinger, Sabine Matovina, André Koch, Dominik Dannehl, et al.was extracted from macrodissected 5 µm paraffin sections using the Maxwell® RSC DNA FFPE Kit and the Maxwell® RSC Instrument (Promega, Madison, WI, USA) and quantified with the Qubit Fluorometer employing the Qubit dsDNA HS Assay Kit

(225) An Investigational Study on the Role of CYP2D6, CYP3A4 and UGTs Genetic Variation on Fesoterodine Pharmacokinetics in Young Healthy VolunteersPharmaceuticals (Basel)September 19, 2024Andrea Rodríguez-Lopez, Dolores Ochoa, Paula Soria-Chacartegui, Samuel Martín-Vilchez, Marcos Navares-Gómez, Eva González-Iglesias, Sergio Luquero-Bueno, Manuel Román, Gina Mejía-Abril, Francisco Abad-SantosADRs. 4.4. Genotyping One EDTA-K2 tube was used for DNA extraction from the total peripheral blood. A Maxwell® RSC instrument (Promega, USA) was used for this purpose. After the extraction, the DNA concentration was measured with

(226) Comparison between standard hematological parameters and blood doping biomarkers in dried blood spots within the athlete population of Swiss Sport IntegrityFrontiers in Sports and Active LivingSeptember 19, 2024Jessica Almeida Oliveira, Francesco Loria, Céline Schobinger, Tiia Kuuranne, Claudia Mumenthaler, Nicolas LeuenbergerCartridges followed by the manufacture instructions, to do an automatically RNA purification with a Maxwell® RSC Instrument (Promega, USA) with a Maxwell® RSC miRNA Plasma and Serum Kit. Elution tubes were recovered and stored

(227) CRISPR-Cas9-mediated genome editing delivered by a single AAV9 vector inhibits HSV-1 reactivation in a latent rabbit keratitis modelMolecular Therapy - Methods & Clinical DevelopmentSeptember 12, 2024Nadia Amrani, Kevin Luk, Pankaj Singh, Mason Shipley, Meltem Isik, Martina Donadoni, Anna Bellizzi, Kamel Khalili, Ilker K. Sariyer, Donna Neumann, et al.homogenizer (Omni International) as described by the manufacturer. The genomic DNA was extracted using Maxwell RSC Instrument with the tissue DNA kit following the manufacturer’s protocol (Promega). In the corneal scarification

(228) A New Variant of the IER3IP1 Gene: The First Case of Microcephaly, Epilepsy, and Diabetes Syndrome 1 from TurkeyJournal of Clinical Research in Pediatric EndocrinologySeptember 4, 2024Elif Söbü, Gül Demet Kaya Kaya Özçora, Elif Yılmaz Yılmaz Güleç, Bahtiyar Şahinoğlu, Feride Tahmiscioğlu Tahmiscioğlu Bucakwas performed according to manufacturer’s instruction (Maxwell RSC Blood DNA Kit, Promega, USA) using Maxwell RSC Instrument (Promega, USA). 30 µL of proteinase K (PK) solution was added into a 200 µL blood sample. Then 300 µL

(229) Refinement of prognostication for IDH ‐mutant astrocytomas using DNA methylation‐based classificationBrain PathologySeptember 1, 2024Teresia Kling, Sandra Ferreyra Vega, Medha Suman, Anna Dénes, Anna Lipatnikova, Stina Lagerström, Thomas Olsson Bontell, Asgeir Store Jakola, Helena CarénDNA methylation profiling DNA was isolated from FFPE tumor samples with the Maxwell® FFPE Plus DNA Kit using the Maxwell® RSC Instrument (Promega, Madison, Wisconsin, USA) as indicated by the developers. Extraction of DNA from FF tumor samples

(230) Colorectal adenocarcinoma with clear cell changes: immunohistological and molecular findings in three casesVirchows ArchivSeptember 1, 2024Andreas Gocht, Carsten Heidel, Jutta Kirfel, Rita Vesce, Pamela Lazar-Karsten, Helen Pasternack, Madelaine Melzer, Phillip Hildebrand, Nicole Warkentin, Hendrik Schimmelpenning, et al.acid extractions. Isolation of genomic DNA was performed using the Maxwell RSC DNA FFPE Kit and the Maxwell RSC instrument (Promega, Fitchburg WI, U.S.A.). DNA samples were quantified using the Qubit fluorimeter (Thermo Fisher,

(231) Clinical phenotype of adult-onset systemic histiocytosis harboring BRAF in-frame deletionsHaematologicaSeptember 1, 2024Matthias Papo, Jérôme Razanamahéry, Malik Da Silva, Zofia Hélias-Rodzewicz, Vsevolod Potapenko, Suzanna Bota, Vanessa Leguy-Seguin, Stéphane Dominique, Raphaël Lhote, Quentin Moyon, et al.follow-up. DNA was extracted as previously described.8 Since 2020, DNA extraction has been automated on a Maxwell® RSC Instrument (Promega, France), with extraction performed according to the supplier’s recommendations. For formalin-fixed paraffin-embedded

(232) Comparison of DNA extraction methods for genotyping equine histidine-rich glycoprotein insertion/deletion polymorphisms using oral mucosa swabs and fecesVeterinary and Animal ScienceSeptember 1, 2024Ryo Muko, Yoshinobu Ojima, Hiroshi Matsuda, Yuko Toishi, Masa-aki Oikawa, Taekyun Shin, Hiroaki Sato, Akane TanakaMadison, WI, USA) according to the manufacturer's protocol. The kit is optimized for the use with the Maxwell RSC Instrument (Promega) which enables the automated extraction of nucleic acids. An oral mucosa swab sample was collected

(233) A new gene signature for endothelial senescence identifies self-RNA sensing by retinoic acid-inducible gene I as a molecular facilitator of vascular agingAging cellSeptember 1, 2024Guduric-Fuchs J, Pedrini E, Bertelli PM, McDonnell S, Pathak V, McLoughlin K, O'Neill CL, Stitt AW, Medina RJ.was based on an absolute log 2‐fold change >1.0 and p < 0.05. RNA from HRMECs was extracted using the Maxwell® RSC instrument and simplyRNA Cells Kit (Promega). Total RNA samples were sent to Edinburgh Genomics (The University

(234) Association of genetic variants with autism spectrum disorder in Japanese children revealed by targeted sequencingFrontiers in GeneticsAugust 30, 2024Yuka Shiota, Tomoaki Nishiyama, Shigeru Yokoyama, Yuko Yoshimura, Chiaki Hasegawa, Sanae Tanaka, Sumie Iwasaki, Mitsuru Kikuchiprocessing Buccal mucosa was collected using a swab. Genomic DNA was extracted from the buccal mucosa using a Maxwell RSC Instrument (Promega, Madison, WI, United States) with the Maxwell RSC Blood DNA Kit (Promega), according to the

(235) Pitfalls When Determining HNA-1 Genotypes and Finding Novel AllelesInternational Journal of Molecular SciencesAugust 22, 2024Kirstine Kløve-Mogensen, Tom Browne, Thure Mors Haunstrup, Rudi Steffensenblood or buccal swabs using the Maxwell RSC Blood DNA Kit or the Maxwell RSC Buccal Swab Kit on the Maxwell RSC instrument according to the manufacturer’s protocol (Promega, Madison, WI, USA). Written and oral informed consent

(236) Case report: Pathology, antimicrobial resistance, and molecular characterization of bovine abortion cases caused by Nocardia farcinica in Korean native cattleFrontiers in Veterinary ScienceAugust 21, 2024Eun-Mi Kim, Chi Sun Yun, You-Chan Bae, Hyunkyoung Lee, Bo-Youn Moon, Kichan Lee, Hye-Young Jeoung, Bok-Kyung Ku, Jongho Kimskeletal muscle, small and large intestines, mesenteric lymph nodes, and endocrine organs) using the Maxwell RSC instrument (Promega, Madison, Wisconsin, United States) with the Maxwell® RSC Blood DNA Kit (Promega) and RSC Viral

(237) Impact of Nanoplastic Particles on Macrophage Inflammation and Intestinal Health in a Mouse Model of Inflammatory Bowel DiseaseNanomaterials (Basel)August 15, 2024Marlene Schwarzfischer, Tano S. Ruoss, Anna Niechcial, Sung Sik Lee, Marcin Wawrzyniak, Andrea Laimbacher, Kirstin Atrott, Roberto Manzini, Marijn Wilmink, Luise Linzmeier, et al.Isolation, RT-PCR, and Real-Time PCR RNA was extracted using the Maxwell RSC simplyRNA Tissue kit and Maxwell RSC instrument (Promega, Walldorf, Germany), following the manufacturer’s guidelines. RNA concentration was determined

(238) Targeting miR-497-5p rescues human keratinocyte dysfunction upon skin exposure to sulfur mustardCell Death & DiseaseAugust 10, 2024Virginia Egea, Karina Lutterberg, Dirk Steinritz, Simone Rothmiller, Konrad Steinestel, Jan Caca, Andreas Nerlich, Helmut Blum, Sarah Reschke, Sajjad Khani, et al.aliquots (0.5 × 106 cells) using the Maxwell® RSC miRNA Tissue Kit (Promega, Madison, WI, USA) on the Maxwell® RSC instrument according to the manufacturer’s instructions. Immediately before library construction, RNA aliquots

(239) Circulating microRNAs as biomarkers for stratifying different phases of liver cancer progression and response to therapyScientific ReportsAugust 9, 2024Lucilla D’Abundo, Cristian Bassi, Elisa Callegari, Farzaneh Moshiri, Paola Guerriero, Angelo Michilli, Fernanda Mora, Andrea Casadei Gardini, Angelo Sangiovanni, Fabio Piscaglia, et al.samples using the Maxwell RSC miRNA Plasma and Serum Kit (Promega Italia, Milano, Italy) in an automated Maxwell RSC Instrument (Promega). Purified RNA was collected in 40 μl of deionized sterile water; hence, each μl contained

(240) Iron capture through CD71 drives perinatal and tumor-associated Treg expansionJCI InsightAugust 8, 2024Ilenia Pacella, Alessandra Pinzon Grimaldos, Alessandra Rossi, Gloria Tucci, Marta Zagaglioni, Elena Potenza, Valeria Pinna, Ivano Rotella, Ilenia Cammarata, Valeria Cancila, et al.concentration (5–10 ng/μL) for all samples was used for library preparation. Samples were subjected to robotic (Maxwell RSC Instrument, Promega) PCR amplification, library preparation, and sequencing according to the Illumina 16S metagenomics

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