Maxwell RSC Instrument from Promega

Product Specs
ItemMaxwell RSC Instrument
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS4500
QuantityEA
Processing Time30 to 60 min (depending on sample type and method)
CapacityUp to 16
Dimensions(WxDxH) 330.2 x 345.2 x 299.7 mm (13 x 13.6 x 11.8 in)
UV LampAvg. lifetime approximately 6,000 hrs
Molecular Weight~85kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(221) Prognostic Factors, FLT-3 Mutations, and Treatment Outcomes with pediatric inspired protocols in adolescent and young adults (AYA) and adult patients with Acute Lymphoblastic LeukemiaActa HaematologicaJanuary 30, 2025Noa Gross Even Zohar, Uriel Oanunu, Noa Gross Even-Zohar, Shlomzion Aumann, Vladimir Vainstein, Alexander Gural, Moshe E Gatt, Arnon Haran, Boaz Nachmiasred blood cell lysis buffer. Cell pellet was subjected for DNA extraction with Maxwell® RSC Blood DNA Kit using Maxwell® RSC Instrument. Detection of FLT-3-ITD Mutation DNA fragment encompassing exons 11–12 of FLT-3 gene was amplified

(222) MUC2 expression modulates immune infiltration in colorectal cancerFrontiers in ImmunologyJanuary 24, 2025Christophe M. Raynaud, Ayesha Jabeen, Eiman I. Ahmed, Satanay Hubrack, Apryl Sanchez, Shimaa Sherif, Ahmad A. Al-Shaibi, Bernice Lo, Jessica Roelands, Davide Bedognetti, et al.(Promega, #AS1390) according to the manufacturer’s recommendations using Maxwell® RSC Instrument (Promega, #AS4500). RNA was recovered with 50 µL of RNase free water and measured with QuantiFluor® RNA System (Promega,

(223) Impact of Genetic Variants on Pregabalin Pharmacokinetics and SafetyPharmaceuticals (Basel)January 23, 2025Sofía Calleja, Andrea Rodríguez-López, Dolores Ochoa, Sergio Luquero, Marcos Navares-Gómez, Manuel Román, Gina Mejia-Abril, Samuel Martín-Vilchez, Francisco Abad-Santos, Pablo Zubiaurconcentrate was resuspended in 0.9% NaCl to preserve the blood sample. DNA extraction was performed using the Maxwell® RSC Instrument (Promega, Madison, WI, USA). Genotyping was carried out using a QuantStudio 12k Flex real-time PCR System

(224) Identification of the clinical and genetic characteristics of gliomas with gene fusions by integrated genomic and transcriptomic analysisEur J Med ResJanuary 23, 2025Guo-Zhong Yi, Hua-Yang Zhang, Tian-Shi Que, Shan-Qiang Qu, Zhi-Yong Li, Song-Tao Qi, Wen-Yan Feng, Guang-Long Huangthe Maxwell RSC Blood DNA Kit (cat# AS1400, Promega, Madison, WI, USA) on a Maxwell RSC system (cat# AS4500, Promega). For formalin-fixed, paraffin-embedded (FFPE) tissue, DNA was extracted using the MagMAX FFPE DNA/RNA Ultra Kit

(225) Transcriptomic analysis reinforces the implication of spatacsin in neuroinflammation and neurodevelopmentSci RepJanuary 18, 2025Liriopé Toupenet Marchesi, Daniel Stockholm, Typhaine Esteves, Marion Leblanc, Nicolas Auger, Julien Branchu, Khalid Hamid El Hachimi, Giovanni StevaninSimplyRNA Tissue - Promega kit). Subsequently, RNA extraction was performed in a Maxwell RSC instrument (AS4500) by transferring the tissue lysates to the first well of each RSC cartridge pre-filled with buffer (Maxwell

(226) Genomic Insights Into Red Squirrels in Scotland Reveal Loss of Heterozygosity Associated With Extreme Founder EffectsEvol ApplJanuary 15, 2025Melissa M. Marr, Emily Humble, Peter W. W. Lurz, Liam A. Wilson, Elspeth Milne, Katie M. Beckmann, Jeffrey Schoenebeck, Uva-Yu-Yan Fung, Andrew C. Kitchener, Kenny Kortland, et al.DNA Extraction and Sequencing DNA was extracted from c. 50 mg of tissue using the automated Promega Maxwell RSC instrument in conjunction with the Maxwell RSC PureFood and GMO Authentication Kit. Double‐stranded, single‐index,

(227) Altered microbiome and metabolome profiling in fearful companion dogs: An exploratory studyPLoS OneJanuary 15, 2025Luigi Sacchettino, Michele Costanzo, Iolanda Veneruso, Valeria D'Argenio, Maria Mayer, Francesco Napolitano, Danila d'Angeloextracted from each collected faecal sample by using the RSC Blood DNA kit and was purified using the Maxwell RSC instrument (both from Promega, Madison, WI, USA). In detail, 100 mg of each sample were treated with 400 μL of

(228) Analysis of Differences in the Classification of Endometrial Cancer Patients in PolandCancers (Basel)January 10, 2025Wiktor Szatkowski, Tomasz Kluz, Małgorzata Cieślak-Steć, Magdalena Śliwińska, Małgorzata Nowak-Jastrząb, Izabela Winkler, Jacek Tomaszewski, Paweł BlecharzDNA isolation:QIAamp DSP DNA FFPE Tissue Kit (Qiagen, Hilden, Germany);Maxwell® RSC DNA FFPE Kit and Maxwell® RSC Instrument (Promega, Madison, WI, USA). 2.4. Molecular Analysis POLE Sequencing Sanger sequencing was conducted

(229) Synthetic GPR84 Agonists in Colorectal Cancer: Effective in THP-1 Cells but Ineffective in BMDMs and MC38 Mouse Tumor ModelsInternational journal of molecular sciencesJanuary 9, 2025Schwarzfischer M, Walker MR, Curcio M, Boshta NM, Marchand A, Soons E, Pöhlmann D, Wawrzyniak M, Morsy Y, Lang S, et al.washed twice with PBS (0.5 mL, pH 7.4). RNA was isolated using a Maxwell RSC simplyRNA Cell kit and the Maxwell RSC instrument (Promega, Madison, WI, USA) according to the manufacturer’s instructions. RNA concentration was quantified,

(230) The integrated stress response drives MET oncogene overexpression in cancersThe EMBO JournalJanuary 7, 2025Marina Cerqua, Marco Foiani, Carla Boccaccio, Paolo M Comoglio, Dogus M Altintasisolated from cells using the Maxwell RSC miRNA Tissue Kit (Catalogue number AS1460, Promega) with the Maxwell RSC Instrument and reverse transcribed using the SuperScript III First-Strand Synthesis System (ThermoFisher Scientific™,

(231) Targeted C-to-T Base Editing in the Arabidopsis Plastid GenomeCurr ProtocJanuary 6, 2025Issei Nakazato, Shin-Ichi Arimuracat. no. MT020‐01H)Zirconia Ball φ5mm (NIKKATO, cat. no. 5‐4060‐33)Multi‐beads Shocker (Yasui Kikai)Maxwell® RSC Instrument (Promega) with compatible computer Additional reagents and equipment for aseptically sowing seeds (see

(232) Molecular Profiling of Sinonasal Adenoid Cystic Carcinoma: Canonical and Noncanonical Gene Fusions and MutationAm J Surg PatholJanuary 6, 2025Alena Skálová, Martina Bradová, Abbas Agaimy, Jan Laco, Cécile Badual, Stephan Ihrler, Ivan Damjanov, Niels J. Rupp, Carlos E. Bacchi, Sarina Mueller, et al.Oncology 500 Kit (Illumina, San Diego, CA). RNA was extracted using the Maxwell RSC DNA FFPE Kit and the Maxwell RSC Instrument (Promega, Madison, WI) according to the manufacturer’s instructions and quantified using the Qubit HS

(233) Whole genome sequencing analysis of enteropathogenic Escherichia coli from human and companion animals in KoreaJournal of Veterinary ScienceJanuary 1, 2025Jae Young Oh, Kyung-Hyo Do, Jae Hong Jeong, SuMin Kwak, Sujin Choe, Dongheui An, Jong-Chan Chae, Kwangjun Lee, Kwang-Won Seohttp://pubmlst.org/biqsdb?db=pubmlst_ecoli_achtman_seqdef. Whole-genome sequencing Genomic DNA from each strain was extracted from overnight cultures using a Maxwell RSC Instrument (Promega, USA), following the manufacturer’s instructions. DNA concentration was determined using a

(234) Mitochondrial trifunctional protein deficiency caused by a deep intronic deletion leading to aberrant splicingJIMD ReportsJanuary 1, 2025Thomas Cassini, Sarah Silverstein, Molly Behan, Cynthia J. Tifft, May Christine Malicdan, David R. Adams, Undiagnosed Diseases Network, Sun‐Young Ahn, Debra S. Regier(A1285901, Gibco/Thermofisher), washed with PBS and pelleted. Total RNA was extracted using Maxwell® RSC (AS4500, Promega) and Maxwell® RSC simplyRNA Cells Kit (AS1390, Promega) following manufacturer's instructions.

(235) Buccal DNA global methylation and cognitive performance in stunted children under five years of ageThe Journal of Biomedical ResearchJanuary 1, 2025Ahmad Rusdan Handoyo Utomo, Yusnita Yusnita, Siti Maulidya Sari, Octaviani Indrasari Ranakusuma, Sunu Bagaskara, Wening Sari, Yulia Suciati, Anggi Puspa Nur Hidayati, Silviatun Nihayah, Catur Anggono Putro, et al.(Maxwell® RSC Blood DNA Kit, Cat. AS1400, Promega, Madison, WI, USA) with magnetic bead technology (Maxwell® RSC Instrument, Promega). The DNA concentration was quantified using spectrophotometry (Tecan Infinite M200 Pro, Switzerland). Epigenetic

(236) The genome of the sapphire damselfish Chrysiptera cyanea: a new resource to support further investigation of the evolution of PomacentridsGigabyteDecember 31, 2024Emma Gairin, Saori Miura, Hiroki Takamiyagi, Marcela Herrera, Vincent Laudetgill, liver, muscle, and brain tissue using the Maxwell® RSC simplyRNA tissue kits and an automated Maxwell® RSC instrument, following manufacturer recommendations (Promega, Cat. No. AS2000, Wisconsin, USA). The quality and

(237) Overexpression of CCL-20 and CXCL-8 genes enhances tumor escape and resistance to cemiplimab, a programmed cell death protein-1 (PD-1) inhibitor, in patients with locally advanced and metastatic cutaneous squamous cell carcinomaOncoImmunologyDecember 31, 2024Vincenzo De Falco, Stefania Napolitano, Renato Franco, Federica Zito Marino, Luigi Formisano, Daniela Esposito, Gabriella Suarato, Rossella Napolitano, Alfonso Esposito, Francesco Caraglia, et al.tumor samples and 3 healthy samples was extracted using Maxwell® RSC RNA FFPE Kit (Promega) by using Maxwell RSC Instrument of Promega Corporation according to manufacturers’ standard protocol. Total RNA quantification and quality

(238) Effect of Genetic Variants on Rosuvastatin Pharmacokinetics in Healthy Volunteers: Involvement of ABCG2, SLCO1B1 and NAT2International Journal of Molecular SciencesDecember 30, 2024Eva González-Iglesias, Clara Méndez-Ponce, Dolores Ochoa, Manuel Román, Gina Mejía-Abril, Samuel Martín-Vilchez, Alejandro de Miguel, Antía Gómez-Fernández, Andrea Rodríguez-Lopez, Paula Soria-Chacartegui, et al.reference formulations. 4.4. Genotyping and Phenotyping DNA was extracted from peripheral blood using a Maxwell® RSC instrument (Promega, Madison, WI, USA). DNA concentration was measured using a Qubit 3.0 fluorometer (ThermoFisher,

(239) Distinct microbes, metabolites, and the host genome define the multi-omics profiles in right-sided and left-sided colon cancerMicrobiomeDecember 28, 2024Lei Liang, Cheng Kong, Jinming Li, Guang Liu, Jinwang Wei, Guan Wang, Qinying Wang, Yongzhi Yang, Debing Shi, Xinxiang Li, et al.a Maxwell RSC Blood DNA Kit (cat# AS1400, Promega, Madison, WI, USA) on a Maxwell RSC system (cat# AS4500, Promega). For formalin-fixed, paraffin-embedded (FFPE) tissue, DNA was extracted using a MagMAX FFPE DNA/RNA Ultra Kit

(240) Air pollution exposure is associated with gene expression in childrenEnvironmental EpigeneticsDecember 24, 2024Siddhartha Das, Amanda Rundblad, Irene Fontes Marques, Ana Goncalves Soares, Vincent W Jaddoe, Martine Vrijheid, Mark Nieuwenhuijsen, Joost Verlouw, Jason Matthews, Kirsten B Holven, et al.before RNA isolation. The Maxwell® RSC miRNA Tissue kits and Maxwell® RSC instrument (artnr. AS1460 and AS4500, respectively; Promega, Madison, WI, USA) were used for extraction of total RNA, including microRNA.

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