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Automated DNA/RNA Purification Sample Preparation Workstations
Maxwell RSC Instrument
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Maxwell RSC Instrument
Maxwell RSC Instrument
Maxwell RSC Instrument from Promega
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Citations:
(881)
Figures:
(1)
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Promega for
Maxwell RSC Instrument
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Description
The Maxwell RSC Instrument is a platform for automated extraction of nucleic acid from a range of sample types. Extraction methods use sample lysis and binding to paramagnetic particles followed by washing steps and elution.
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Maxwell RSC Instrument
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
AS4500
Quantity
EA
Processing Time
30 to 60 min (depending on sample type and method)
Capacity
Up to 16
Dimensions
(WxDxH) 330.2 x 345.2 x 299.7 mm (13 x 13.6 x 11.8 in)
UV Lamp
Avg. lifetime approximately 6,000 hrs
Molecular Weight
~85kDa
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Promega
2800 Woods Hollow Rd
Madison
,
WI
53711
United States
Phone
: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516
Email: custserv@promega.com
techserv@promega.com
Company Profile
Website:
http://www.promega.com
Citations (881)
(261)
Targeting ErbB and tankyrase1/2 prevent the emergence of drug-tolerant persister cells in ALK-positive lung cancer
npj Precision Oncology
November 17, 2024
Takaaki Fujimura, Koh Furugaki, Hayato Mizuta, Satoshi Muraoka, Makoto Nishio, Jun Adachi, Ken Uchibori, Eisaku Miyauchi, Hidetoshi Hayashi, Ryohei Katayama,
et al.
using STAR, and read count data was obtained with prepDE.py.
RT-PCR assay
mRNA was purified using the
Maxwell RSC instrument
(
Promega
), and complementary DNA (cDNA) was synthesized using the PrimeScript RT reagent kit (Takara
(262)
Sclerosing mucoepidermoid carcinoma of salivary glands
Virchows Archiv
November 14, 2024
Bacem Khalele Othman, Martina Bradová, Roderick H. W. Simpson, Jan Laco, Abbas Agaimy, Miguel Rito, Stephan Ihrler, Petr Steiner, Petr Grossmann, Veronika Hájková,
et al.
Oncology 500 Kit (Illumina, San Diego, CA). RNA was extracted using the
Maxwell RSC DNA FFPE Kit
and the
Maxwell RSC Instrument
(
Promega
, Madison, WI) according to the manufacturer’s instructions and quantified using the Qubit HS
(263)
Dynamics of cell-free tumor DNA correlate with early MRI response during chemoradiotherapy in rectal cancer
Radiation Oncology
November 6, 2024
Kerstin Clasen, Cihan Gani, Leon Schuetz, Stephan Clasen, Nadja Ballin, Irina Bonzheim, Michael Orth, Stephan Ossowski, Olaf Riess, Maximilian Niyazi,
et al.
one to ten 5 μm paraffin sections and DNA was extracted using the
Maxwell RSC DNA FFPE Kit
and the
Maxwell RSC Instrument
(
Promega
, Madison, WI, USA). A Covaris E220 Ultrasonicatior was used to shear 200 ng genomic DNA into
(264)
Single Laboratory Evaluation of the Q20+ Nanopore Sequencing Kit for Bacterial Outbreak Investigations
International Journal of Molecular Sciences
November 5, 2024
Maria Hoffmann, Jay Hee Jang, Sandra M. Tallent, Narjol Gonzalez-Escalona
Measurement Genomic DNA from each
strain
was extracted using either the Maxwell RSC
Cultured Cell
DNA Kit with a
Maxwell RSC Instrument
(
Promega Corporation
, Madison, WI, USA) according to the manufacturer’s instructions for
Gram-negative bacteria
(265)
Association of clonal haematopoiesis with recurrent venous thromboembolism: A case–control study
British Journal of Haematology
November 5, 2024
Cornelia Englisch, Rafaela Vostatek, Theresa Schramm, Christoph J. Binder, Ingrid Pabinger, Roland Jäger, Cihan Ay
after the most recent VTE event as described previously, 20 using the
Maxwell® RSC Blood DNA Kit
on a
Maxwell® RSC Instrument
(all
Promega
, Vienna, Austria). Furthermore, routine laboratory parameters were measured centrally at
(266)
A chromosome-level genome assembly of a model conifer plant, the Japanese cedar, Cryptomeria japonica D. Don
BMC Genomics
November 5, 2024
Takeshi Fujino, Katsushi Yamaguchi, Toshiyuki T. Yokoyama, Toshiya Hamanaka, Yoritaka Harazono, Hiroaki Kamada, Wataru Kobayashi, Tokuko Ujino-Ihara, Kentaro Uchiyama, Asako Matsumoto,
et al.
with 200 µL of
Lysis Buffer
of the
Maxwell RSC Plant RNA Kit
(
Promega
). RNA was then extracted using a
Maxwell RSC Instrument
(
Promega
) according to the manufacturer’s instructions. The extracted RNA was sequenced by Macrogen
(267)
DNA methylation biomarker panels for differentiating various liver adenocarcinomas, including hepatocellular carcinoma, cholangiocarcinoma, colorectal liver metastases and pancreatic adenocarcinoma liver metastases
Clinical Epigenetics
November 4, 2024
Tina Draškovič, Branislava Ranković, Nina Zidar, Nina Hauptman
isolation was performed using the commercially available
Maxwell RSC FFPE Plus DNA Kit
(
Promega
) on the
Maxwell® RSC Instrument
(
Promega
) according to the manufacturer's instructions. Quantification and quality of DNA isolates were
(268)
Gut Bacteria Metabolize Natural and Synthetic Steroid Hormones via the Reductive OsrABC Pathway
bioRxiv
October 19, 2024
Christian Jacoby, Kaylie Scorza, Lia Ecker, Mary McMillin, Ramanujam Ramaswamy, Anitha Sundararajan, Ashley M. Sidebottom, Huaiying Lin, Keith Dufault-Thompson, Brantley Hall,
et al.
subsequent processing. Cell pellets were thawed and total RNA from biological replicates extracted using the
Maxwell RSC instrument
(
Promega
). Extracted RNA was quantified using Qubit, and integrity was measured using TapeStation (Agilent
(269)
Tenacibaculum maritimum can boost inflammation in Dicentrarchus labrax upon peritoneal injection but cannot trigger tenacibaculosis disease
Frontiers in Immunology
October 14, 2024
Inês A. Ferreira, Paulo Santos, Javier Sanz Moxó, Carla Teixeira, Ana do Vale, Benjamin Costas
µL of lysis buffer to the samples, all total RNA isolations were performed by Maxwell® RSC (Cat. #
AS4500
). RNA samples were quantified, and purity was evaluated by
spectrophotometry
using
DeNovix
DS-11 FX
(270)
Tumour-informed liquid biopsies to monitor advanced melanoma patients under immune checkpoint inhibition
Nature Communications
October 9, 2024
Christopher Schroeder, Sergios Gatidis, Olga Kelemen, Leon Schütz, Irina Bonzheim, Francesc Muyas, Peter Martus, Jakob Admard, Sorin Armeanu-Ebinger, Brigitte Gückel,
et al.
paraffin sections depending on tumour size. DNA was extracted using the
Maxwell RSC DNA FFPE Kit
and the
Maxwell RSC Instrument
(
Promega
, Madison, WI, USA) according to the manufacturer’s instructions. There was no pre-defined threshold
(271)
A Novel Chimeric Oncolytic Virus Mediates a Multifaceted Cellular Immune Response in a Syngeneic B16 Melanoma Model
Cancers (Basel)
October 6, 2024
Sonja Glauß, Victoria Neumeyer, Lorenz Hanesch, Janina Marek, Nina Hartmann, Gabriela M. Wiedemann, Jennifer Altomonte
France). RNA was isolated using a
Maxwell RSC SimplyRNA tissue kit
(
Promega
, Madison, WI, USA) and the
Maxwell RSC instrument
(
Promega
). GoTaq
1-step RT-qPCR
Kit (Promega) and a
7500 Real Time PCR system
(
Applied Biosystems
, Foster
(272)
Conserved allomorphs of MR1 drive the specificity of MR1-restricted TCRs
Frontiers in Oncology
October 3, 2024
Terri V. Cornforth, Nathifa Moyo, Suzanne Cole, Emily P. S. Lam, Tatiana Lobry, Ron Wolchinsky, Angharad Lloyd, Katarzyna Ward, Eleanor M. Denham, Giulia Masi,
et al.
(Psi) and a reference gene (RPP30) measured from the same sample. Genomic DNA was extracted using a
Maxwell RSC Instrument
(
Promega
, USA) following the manufacturer’s protocol. Extracted DNA was digested using an
EcoRI HF
Restriction
(273)
Targeted phage hunting to specific Klebsiella pneumoniae clinical isolates is an efficient antibiotic resistance and infection control strategy
Microbiology Spectrum
October 3, 2024
Celia Ferriol-González, Robby Concha-Eloko, Mireia Bernabéu-Gimeno, Felipe Fernández-Cuenca, Javier E. Cañada-García, Silvia García-Cobos, Rafael Sanjuán, Pilar Domingo-Calap
Clean & Concentrator 5-Kit (Zymo) or Maxwell PureFood GMO and Authentication Kit (Promega) with the
Maxwell RSC Instrument
(
Promega
).
Phage
sequencing
and assembly
Sequencing
libraries were prepared using the Illumina Nextera
(274)
Aberrant p53 immunostaining patterns in breast carcinoma of no special type strongly correlate with presence and type of TP53 mutations
Virchows Archiv
October 1, 2024
Hannes Armbruster, Tilman Schotte, Isabell Götting, Mathis Overkamp, Massimo Granai, Lea Louise Volmer, Veronika Bahlinger, Sabine Matovina, André Koch, Dominik Dannehl,
et al.
was extracted from macrodissected 5 µm paraffin sections using the
Maxwell® RSC DNA FFPE Kit
and the
Maxwell® RSC Instrument
(
Promega
, Madison, WI, USA) and quantified with the
Qubit Fluorometer
employing the
Qubit dsDNA HS Assay Kit
(275)
Comparison between standard hematological parameters and blood doping biomarkers in dried blood spots within the athlete population of Swiss Sport Integrity
Frontiers in Sports and Active Living
September 19, 2024
Jessica Almeida Oliveira, Francesco Loria, Céline Schobinger, Tiia Kuuranne, Claudia Mumenthaler, Nicolas Leuenberger
Cartridges followed by the manufacture instructions, to do an automatically
RNA purification
with a
Maxwell® RSC Instrument
(
Promega
, USA) with a
Maxwell® RSC miRNA Plasma and Serum Kit
. Elution tubes were recovered and stored
(276)
An Investigational Study on the Role of CYP2D6, CYP3A4 and UGTs Genetic Variation on Fesoterodine Pharmacokinetics in Young Healthy Volunteers
Pharmaceuticals (Basel)
September 19, 2024
Andrea Rodríguez-Lopez, Dolores Ochoa, Paula Soria-Chacartegui, Samuel Martín-Vilchez, Marcos Navares-Gómez, Eva González-Iglesias, Sergio Luquero-Bueno, Manuel Román, Gina Mejía-Abril, Francisco Abad-Santos
ADRs. 4.4.
Genotyping
One EDTA-K2 tube was used for DNA extraction from the total peripheral blood. A
Maxwell® RSC instrument
(
Promega
, USA) was used for this purpose. After the extraction, the DNA concentration was measured with
(277)
CRISPR-Cas9-mediated genome editing delivered by a single AAV9 vector inhibits HSV-1 reactivation in a latent rabbit keratitis model
Molecular Therapy - Methods & Clinical Development
September 12, 2024
Nadia Amrani, Kevin Luk, Pankaj Singh, Mason Shipley, Meltem Isik, Martina Donadoni, Anna Bellizzi, Kamel Khalili, Ilker K. Sariyer, Donna Neumann,
et al.
homogenizer (Omni International) as described by the manufacturer. The genomic DNA was extracted using
Maxwell RSC Instrument
with the tissue DNA kit following the manufacturer’s protocol (
Promega
). In the corneal scarification
(278)
Discovery of Novel TULP4/ACTN4/EWSR1/ACTB::MYB and ESRRG::DNM3 Fusions Expands Molecular Landscape of Adenoid Cystic Carcinoma Beyond Fusions Between MYB/MYBL1 and NFIB Genes
Am J Surg Pathol
September 4, 2024
Alena Skálová, Natálie Klubíčková, Martina Bradová, Abbas Agaimy, Niels J. Rupp, Ivan Damjanov, Georgina Kolnikova, Petr Martínek, Petr Šteiner, Petr Grossmann,
et al.
TruSight Oncology 500 Kit (Illumina). RNA was extracted using the
Maxwell RSC DNA FFPE Kit
and the
Maxwell RSC Instrument
(
Promega
) according to the manufacturer’s instructions and quantified using the Qubit HS RNA
Assay
Kit
(279)
A New Variant of the IER3IP1 Gene: The First Case of Microcephaly, Epilepsy, and Diabetes Syndrome 1 from Turkey
Journal of Clinical Research in Pediatric Endocrinology
September 4, 2024
Elif Söbü, Gül Demet Kaya Kaya Özçora, Elif Yılmaz Yılmaz Güleç, Bahtiyar Şahinoğlu, Feride Tahmiscioğlu Tahmiscioğlu Bucak
was performed according to manufacturer’s instruction (
Maxwell RSC Blood DNA Kit
,
Promega
, USA) using
Maxwell RSC Instrument
(
Promega
, USA). 30 µL of
proteinase K (PK) solution
was added into a 200 µL blood sample. Then 300 µL
(280)
Clinical phenotype of adult-onset systemic histiocytosis harboring
BRAF
in-frame deletions
Haematologica
September 1, 2024
Matthias Papo, Jérôme Razanamahéry, Malik Da Silva, Zofia Hélias-Rodzewicz, Vsevolod Potapenko, Suzanna Bota, Vanessa Leguy-Seguin, Stéphane Dominique, Raphaël Lhote, Quentin Moyon,
et al.
follow-up. DNA was extracted as previously described.8 Since 2020, DNA extraction has been automated on a
Maxwell® RSC Instrument
(
Promega
, France), with extraction performed according to the supplier’s recommendations. For
formalin-fixed paraffin-embedded
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Maxwell RSC Instrument from Promega
Supplier Page
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