Maxwell RSC Instrument from Promega

Product Specs
ItemMaxwell RSC Instrument
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS4500
QuantityEA
Processing Time30 to 60 min (depending on sample type and method)
CapacityUp to 16
Dimensions(WxDxH) 330.2 x 345.2 x 299.7 mm (13 x 13.6 x 11.8 in)
UV LampAvg. lifetime approximately 6,000 hrs
Molecular Weight~85kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(261) Impact of Nanoplastic Particles on Macrophage Inflammation and Intestinal Health in a Mouse Model of Inflammatory Bowel DiseaseNanomaterials (Basel)August 15, 2024Marlene Schwarzfischer, Tano S. Ruoss, Anna Niechcial, Sung Sik Lee, Marcin Wawrzyniak, Andrea Laimbacher, Kirstin Atrott, Roberto Manzini, Marijn Wilmink, Luise Linzmeier, et al.Isolation, RT-PCR, and Real-Time PCR RNA was extracted using the Maxwell RSC simplyRNA Tissue kit and Maxwell RSC instrument (Promega, Walldorf, Germany), following the manufacturer’s guidelines. RNA concentration was determined

(262) Targeting miR-497-5p rescues human keratinocyte dysfunction upon skin exposure to sulfur mustardCell Death & DiseaseAugust 10, 2024Virginia Egea, Karina Lutterberg, Dirk Steinritz, Simone Rothmiller, Konrad Steinestel, Jan Caca, Andreas Nerlich, Helmut Blum, Sarah Reschke, Sajjad Khani, et al.aliquots (0.5 × 106 cells) using the Maxwell® RSC miRNA Tissue Kit (Promega, Madison, WI, USA) on the Maxwell® RSC instrument according to the manufacturer’s instructions. Immediately before library construction, RNA aliquots

(263) Circulating microRNAs as biomarkers for stratifying different phases of liver cancer progression and response to therapyScientific ReportsAugust 9, 2024Lucilla D’Abundo, Cristian Bassi, Elisa Callegari, Farzaneh Moshiri, Paola Guerriero, Angelo Michilli, Fernanda Mora, Andrea Casadei Gardini, Angelo Sangiovanni, Fabio Piscaglia, et al.samples using the Maxwell RSC miRNA Plasma and Serum Kit (Promega Italia, Milano, Italy) in an automated Maxwell RSC Instrument (Promega). Purified RNA was collected in 40 μl of deionized sterile water; hence, each μl contained

(264) In pancreatic cancer patients, chemotherapy reshapes the gene expression profile and antigen receptor repertoire of T lymphocytes and enhances their effector response to tumor-associated antigensFrontiers in ImmunologyAugust 8, 2024Silvia Brugiapaglia, Sara Bulfamante, Claudia Curcio, Maddalena Arigoni, Raffaele Calogero, Lisa Bonello, Elisa Genuardi, Rosella Spadi, Maria Antonietta Satolli, Donata Campra, et al.extraction following the manufacturer’s instructions with Maxwell® RSC simplyRNA Cells Kit compatible with Maxwell® RSC Instrument (Promega, Madison, WI). The concentration of mRNA was evaluated with QuantiFluor® RNA System (Promega,

(265) Iron capture through CD71 drives perinatal and tumor-associated Treg expansionJCI InsightAugust 8, 2024Ilenia Pacella, Alessandra Pinzon Grimaldos, Alessandra Rossi, Gloria Tucci, Marta Zagaglioni, Elena Potenza, Valeria Pinna, Ivano Rotella, Ilenia Cammarata, Valeria Cancila, et al.concentration (5–10 ng/μL) for all samples was used for library preparation. Samples were subjected to robotic (Maxwell RSC Instrument, Promega) PCR amplification, library preparation, and sequencing according to the Illumina 16S metagenomics

(266) The intron binding protein EMB-4 is an opposite regulator of cold and high temperature tolerance in Caenorhabditis elegansPNAS NexusAugust 5, 2024Akane Ohta, Yuki Sato, Kazuho Isono, Takuma Kajino, Keisuke Tanaka, Teruaki Taji, Atsushi Kuharawas extracted from the crushed worms in the homogenization solution using the RSC Tissue RNA kit and Maxwell RSC instrument (Promega). RNA-seq libraries were prepared using TruSeq RNA sample prep kit v2 (Illumina) according

(267) Phishing vulnerability compounded by older age, apolipoprotein E e4 genotype, and lower cognitionPNAS NexusAugust 5, 2024Didem Pehlivanoglu, Alayna Shoenfelt, Ziad Hakim, Amber Heemskerk, Jialong Zhen, Mario Mosqueda, Robert C Wilson, Matthew Huentelman, Matthew D Grilli, Gary Turner, et al.completely at room temperature for a minimum of 3 hours. DNA was isolated from the Mitra device using the Maxwell RSC instrument (Promega, Madison, WI, USA) according to the manufacturer's instructions in the customized Product Application

(268) Lipolysis pathways modulate lipid mediator release and endocannabinoid system signaling in dairy cows’ adipocytesJournal of Animal Science and BiotechnologyAugust 3, 2024Madison N. Myers, Miguel Chirivi, Jeff C. Gandy, Joseph Tam, Maya Zachut, G. Andres Contreras°C. Prior to extraction, samples were thawed on ice. RNA was extracted and purified using the automated Maxwell® RSC Instrument (Promega Corp.) and simplyRNA Cells Kit (Promega Corp.) according to the manufacturer’s instructions.

(269) SMARCB1-deficient sinonasal adenocarcinoma: a rare variant of SWI/SNF-deficient malignancy often misclassified as high-grade non-intestinal-type sinonasal adenocarcinoma or myoepithelial carcinomaVirchows ArchivAugust 1, 2024Alena Skálová, Touraj Taheri, Martina Bradová, Tomáš Vaněček, Alessandro Franchi, David Slouka, Tomáš Kostlivý, Gisele de Rezende, Jaroslav Michálek, Natálie Klubíčková, et al.Oncology 500 Kit (Illumina, San Diego, CA). RNA was extracted using the Maxwell RSC DNA FFPE Kit and the Maxwell RSC Instrument (Promega, Madison, WI) according to the manufacturer’s instructions and quantified using the Qubit HS

(270) α7 nicotinic receptor activation mitigates herpes simplex virus type 1 infection in microglia cellsAntiviral ResearchAugust 1, 2024Shih-Heng Chen, Joanne C. Damborsky, Belinda C. Wilson, Rick D. Fannin, James M. Ward, Kevin E. Gerrish, Bo He, Negin P. Martin, Jerrel L. Yakelselection for 48 hours prior to use. 2.5. RNA analysis Total RNA was extracted from cultures using the Maxwell RSC instrument and Maxwell miRNA Tissue Kit (Promega, Cat. # AS1460) according to the manufacturer's protocol. Reverse

(271) Rapid response to selpercatinib in RET fusion positive pancreatic neuroendocrine carcinoma confirmed by smartwatchnpj Precision OncologyJuly 31, 2024Barbara Deschler-Baier, Markus Krebs, Matthias Kroiss, Manik Chatterjee, Daniel Gundel, Christian Kestler, Alexander Kerscher, Volker Kunzmann, Silke Appenzeller, Katja Maurus, et al.ensure adequate tumor cellularity for analysis. DNA and RNA were extracted from FFPE tissue using a Maxwell®RSC instrument (Promega GmbH, Walldorf, Germany) according to standard protocols. Genomic profiling was performed using

(272) miR-29a-3p orchestrates key signaling pathways for enhanced migration of human mesenchymal stem cellsCell Communication and SignalingJuly 17, 2024Dayeon Kang, Taehwan Kim, Ga-Eun Choi, Arum Park, Jin Yoon, Jinho Yu, Nayoung SuhQuantitative real-time PCR Total RNA was isolated from cultured cells using the Maxwell RSC miRNA Isolation Kit (Promega) on a Maxwell RSC Instrument (Promega) according to the manufacturer’s instructions. cDNA was prepared using random hexamers and

(273) Immunogenic Comparison of Nucleic Acid-Based Vaccines Administered by Pyro-Drive Jet InjectorVaccines (Basel)July 9, 2024Jiayu A. Tai, Tomoyuki Nishikawa, Hiroki Hayashi, Yu-Diao Kuan, Kunihiko Yamashita, Hironori Nakagamiwas extracted from all the samples using the Maxwell RSC simplyRNA Tissue Kit (#AS1340, Promega) and Maxwell RSC Instrument (Promega). Total RNA (1 µg) was reverse transcribed into cDNA using the ReverTra Ace qPCR RT kit (#FSQ-201,

(274) Nematode Galectin Inhibits Basophilic Leukaemia RBL-2H3 Cells Apoptosis in IgE-Mediated ActivationInternational Journal of Molecular SciencesJuly 6, 2024Marta Maruszewska-Cheruiyot, Ludmiła Szewczak, Katarzyna Krawczak-Wójcik, Michael James Stear, Katarzyna Donskow-ŁysoniewskaGene Expression Microarray RNA was extracted from cells using Maxwell® RSC simply RNA Blood Kit on a Maxwell RSC Instrument (Promega, Madison, WA, USA) according to the manufacturer’s instructions. Concentration and quality

(275) Diversity and Pathogenicity of Botryosphaeriaceae Species Isolated from Olives in Istria, Croatia, and Evaluation of Varietal ResistancePlants (Basel)July 1, 2024Elena Petrović, Karolina Vrandečić, Andreina Belušić Vozila, Jasenka Ćosić, Sara Godenacolony margins was aseptically sampled using a sterile laboratory needle for genomic DNA extraction. Maxwell® RSC Instrument (Promega, Madison, WI, USA) and Maxwell® RSC Plant DNA Kit (Promega, Madison, WI, USA) were used to

(276) Seltene Differenzialdiagnose bei osteolytischer Läsion des Unterkiefers einer jungen ErwachsenenPathologie (Heidelb)July 1, 2024Hyunkyu Shin, Andreas Naros, Sinja Kieninger, Joachim Polligkeit, Falko Fend, Jakob MillaHierzu wurde RNA aus Formalin-fixierten Paraffinschnitten mit dem Maxwell® RSC RNA FFPE Kit und dem Maxwell® RSC Instrument (Promega, Madison, WI, USA) gemäß den Anweisungen des Herstellers extrahiert. Zur Analyse von Fusionsgenen

(277) Cannabinol Regulates the Expression of Cell Cycle-Associated Genes in Motor Neuron-like NSC-34: A Transcriptomic AnalysisBiomedicinesJune 17, 2024Alessandra Trainito, Agnese Gugliandolo, Luigi Chiricosta, Stefano Salamone, Federica Pollastro, Emanuela Mazzon, Maria LuiMaxwell® RSC simplyRNA Cells Kit (#AS1390, Promega, Madison, WI, USA) was used to extract the total RNA with a Maxwell® RSC instrument. Library preparation was carried out with TruSeq® RNA Exome protocol (Illumina, San Diego, CA, USA)

(278) Linking preterm infant gut microbiota to nasograstric enteral feeding tubes: exploring potential interactions and microbial strain transmissionFrontiers in PediatricsJune 17, 2024J. Jara, C. Alba, R. Del Campo, L. Fernández, M. Sáenz de Pipaón, J. M. Rodríguez, B. Orgazprocessing. 2.4 DNA extraction from fecal samples DNA extraction from feces was performed using a Maxwell® RSC instrument and a Maxwell® RSC PureFood GMO and Authentication Kit (Promega Corporation, Madison, WI, USA) as described

(279) Application of comprehensive molecular genetic profiling in precision cancer medicine, Hungarian experiencesActa OncologicaJune 16, 2024Erika Tóth, Zsófia Kürönya, Edina Soós, Tamás Pintér, Henriett Butz, Zsolt Horváth, Erzsébet Csernák, Vince Kornél Grolmusz, Judit Székely, Tamás Straussz, et al.macrodissection. Nucleic acid isolation was performed using either Maxwell RSC DNA/RNA FFPE Kit on Maxwell RSC Instrument (Promega, USA) or MagMAX FFPE DNA/RNA Ultra Kit on the KingFisher Duo Prime purification system (Thermo Fisher Scientific

(280) Application of comprehensive molecular genetic profiling in precision cancer medicine, Hungarian experiencesActa OncolJune 16, 2024Erika Tóth, Zsófia Küronya, Edina Soós, Tamás Pintér, Henriett Butz, Zsolt Horváth, Erzsébet Csernák, Vince Kornél Grolmusz, Judit Székely, Tamás Strausz, et al.macrodissection. Nucleic acid isolation was performed using either Maxwell RSC DNA/RNA FFPE Kit on Maxwell RSC Instrument (Promega, USA) or MagMAX FFPE DNA/RNA Ultra Kit on the KingFisher Duo Prime purification system (Thermo Fisher Scientific

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