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Automated DNA/RNA Purification Sample Preparation Workstations
Maxwell RSC Instrument
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Maxwell RSC Instrument
Maxwell RSC Instrument
Maxwell RSC Instrument from Promega
Be the first to write a review!
Citations:
(802)
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(1)
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Maxwell RSC Instrument
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Description
The Maxwell RSC Instrument is a platform for automated extraction of nucleic acid from a range of sample types. Extraction methods use sample lysis and binding to paramagnetic particles followed by washing steps and elution.
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Maxwell RSC Instrument
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
AS4500
Quantity
EA
Processing Time
30 to 60 min (depending on sample type and method)
Capacity
Up to 16
Dimensions
(WxDxH) 330.2 x 345.2 x 299.7 mm (13 x 13.6 x 11.8 in)
UV Lamp
Avg. lifetime approximately 6,000 hrs
Molecular Weight
~85kDa
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Promega
2800 Woods Hollow Rd
Madison
,
WI
53711
United States
Phone
: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516
Email: custserv@promega.com
techserv@promega.com
Company Profile
Website:
http://www.promega.com
Citations (802)
(201)
Discovery of Novel TULP4/ACTN4/EWSR1/ACTB::MYB and ESRRG::DNM3 Fusions Expands Molecular Landscape of Adenoid Cystic Carcinoma Beyond Fusions Between MYB/MYBL1 and NFIB Genes
American Journal of Surgical Pathology
December 1, 2024
Alena Skálová, Natálie Klubíčková, Martina Bradová, Abbas Agaimy, Niels J. Rupp, Ivan Damjanov, Georgina Kolnikova, Petr Martínek, Petr Šteiner, Petr Grossmann,
et al.
TruSight Oncology 500 Kit (Illumina). RNA was extracted using the
Maxwell RSC DNA FFPE Kit
and the
Maxwell RSC Instrument
(
Promega
) according to the manufacturer’s instructions and quantified using the Qubit HS RNA
Assay
Kit
(202)
Comparative assessment of three commercial kits and in house optimized PCR assays for GMO screening in food and feed
MethodsX
December 1, 2024
Daniela Verginelli, Cinzia Quarchioni, Katia Spinella, Davide La Rocca, Pamela Bonini, Cristiana Fusco, Marisa Misto, Stefania Peddis, Lorella Peroni, Ugo Marchesi
method with minor modifications on buffer volume and, RSC PureFood GMO performed DNA extraction by using
Maxwell®RSC Instrument
(
Promega
Madison, WI, USA) as
nucleic acid purification
platform, according to the manufacturer's instructions.
(203)
Viral coexistence and insertional mutations in the ORF8 region of SARS-CoV-2: A possible mechanism of nucleotide insertion
Virus research
December 1, 2024
Kurose M, Yamamoto A, Elsayed AMA, Lawal-Ayinde BM, Nomura T, Higashiura A, Irie T, Fukushi M, Kanda M, Tahara H,
et al
.
analysis, viral RNA was purified from swab samples or
viruses
amplified in VeroE6/TMPRSS2 cells by using
Maxwell RSC Instrument
(
Promega
, Milwaukee, WI, USA). cDNA preparation and
amplification
were performed following protocols
(204)
DNA Methylation Panels for the Differentiation of Lung and Gastric Adenocarcinomas from Other Common Primary Adenocarcinomas
Cancers (Basel)
November 29, 2024
Tina Draškovič, Lara Omahen, Maja Jerše, Nina Zidar, Nina Hauptman
Bisulfite Conversion Total DNA was extracted using the automated
Maxwell® RSC FFPE Plus DNA Kit
with the
Maxwell® RSC Instrument
(
Promega Corporation
, Madison, WI, USA). Isolation followed the manufacturer’s instructions, utilizing
(205)
Upregulation of ABLIM1 Differentiates Intrahepatic Cholangiocarcinoma from Hepatocellular Carcinoma and Both Colorectal and Pancreatic Adenocarcinoma Liver Metastases
Genes (Basel)
November 28, 2024
Tina Draškovič, Branislava Ranković, Nina Zidar, Nina Hauptman
FFPE tissue blocks. RNA isolation from the punches was performed using the customized protocol on the
Maxwell® RSC Instrument
(
Promega Corporation
, Madison, WI, USA). The protocol combined the Maxwell RSC FFPE Plus RNA Kit (Promega
(206)
Human VCP mutant ALS/FTD microglia display immune and lysosomal phenotypes independently of GPNMB
Molecular Neurodegeneration
November 26, 2024
Benjamin E. Clarke, Oliver J. Ziff, Giulia Tyzack, Marija Petrić Petrić Howe, Yiran Wang, Pierre Klein, Claudia A. Smith, Cameron A. Hall, Adel Helmy, Michael Howell,
et al.
Promega
Maxwell RSC simplyRNA cells kit
including DNase treatment was used for RNA extractions using the
Maxwell RSC instrument
. A nanodrop was used to assess RNA concentration and the 260/280 ratio. RNA integrity was assessed using
(207)
Analytical Validation and Performance Evaluation of Amplicon-Based Next-Generation Sequencing Assays for Detecting ERBB2 and Other Gene Amplifications in Solid Tumors
Cancers (Basel)
November 23, 2024
Ekaterina Olkhov-Mitsel, Danny Chan, Kenneth J. Craddock, August Lin, Grace Luk, Rashmi S. Goswami, Hong Wang, Anna Plotkin, Sharon Nofech-Mozes, David M. Hwang,
et al.
(gDNA) was extracted from archival FFPE
tumor
samples (10 slides, 5 micron each) using the
Promega
Maxwell RSC Instrument
(Promega, Madison, WI, USA). The extracted gDNA was quantified via Qubit 3 Fluorometer using Qubit dsDNA
(208)
Isolation and Detection of the Emerging Pathogen Escherichia albertii in Clinical Stool Samples and the Potential Transmission by Meat Samples in Retail
Microorganisms
November 23, 2024
Muhammad Zeeshan Zafar, Klara De Rauw, Anne-Marie Van den Abeele, Marie Joossens, Lore Heyvaert, Kurt Houf
Maxwell RSC Cultured Cells DNA kit
(
AS1620
,
Promega
, Madison, WI, USA) on the
Maxwell RSC instrument
(
AS4500
,
Promega
, USA). Paired-end 2 × 150 bp
sequencing
was performed using the
Illumina
Novaseq X plus
platform
(209)
Nanopore Sequencing Allows Recovery of High-Quality Completely Closed Genomes of All Cronobacter Species from Powdered Infant Formula Overnight Enrichments
Microorganisms
November 22, 2024
Narjol Gonzalez-Escalona, Hee Jin Kwon, Yi Chen
DNA from each PIF inoculated sample was extracted using the
Maxwell RSC Cultured Cells DNA kit
with a
Maxwell RSC Instrument
(
Promega Corporation
, Madison, WI, USA) according to the manufacturer’s instructions for
Gram-negative bacteria
(210)
Targeting ErbB and tankyrase1/2 prevent the emergence of drug-tolerant persister cells in ALK-positive lung cancer
npj Precision Oncology
November 17, 2024
Takaaki Fujimura, Koh Furugaki, Hayato Mizuta, Satoshi Muraoka, Makoto Nishio, Jun Adachi, Ken Uchibori, Eisaku Miyauchi, Hidetoshi Hayashi, Ryohei Katayama,
et al.
using STAR, and read count data was obtained with prepDE.py.
RT-PCR assay
mRNA was purified using the
Maxwell RSC instrument
(
Promega
), and complementary DNA (cDNA) was synthesized using the PrimeScript RT reagent kit (Takara
(211)
Sclerosing mucoepidermoid carcinoma of salivary glands
Virchows Archiv
November 14, 2024
Bacem Khalele Othman, Martina Bradová, Roderick H. W. Simpson, Jan Laco, Abbas Agaimy, Miguel Rito, Stephan Ihrler, Petr Steiner, Petr Grossmann, Veronika Hájková,
et al.
Oncology 500 Kit (Illumina, San Diego, CA). RNA was extracted using the
Maxwell RSC DNA FFPE Kit
and the
Maxwell RSC Instrument
(
Promega
, Madison, WI) according to the manufacturer’s instructions and quantified using the Qubit HS
(212)
Dynamics of cell-free tumor DNA correlate with early MRI response during chemoradiotherapy in rectal cancer
Radiation Oncology
November 6, 2024
Kerstin Clasen, Cihan Gani, Leon Schuetz, Stephan Clasen, Nadja Ballin, Irina Bonzheim, Michael Orth, Stephan Ossowski, Olaf Riess, Maximilian Niyazi,
et al.
one to ten 5 μm paraffin sections and DNA was extracted using the
Maxwell RSC DNA FFPE Kit
and the
Maxwell RSC Instrument
(
Promega
, Madison, WI, USA). A Covaris E220 Ultrasonicatior was used to shear 200 ng genomic DNA into
(213)
Association of clonal haematopoiesis with recurrent venous thromboembolism: A case–control study
British Journal of Haematology
November 5, 2024
Cornelia Englisch, Rafaela Vostatek, Theresa Schramm, Christoph J. Binder, Ingrid Pabinger, Roland Jäger, Cihan Ay
after the most recent VTE event as described previously, 20 using the
Maxwell® RSC Blood DNA Kit
on a
Maxwell® RSC Instrument
(all
Promega
, Vienna, Austria). Furthermore, routine laboratory parameters were measured centrally at
(214)
A chromosome-level genome assembly of a model conifer plant, the Japanese cedar, Cryptomeria japonica D. Don
BMC Genomics
November 5, 2024
Takeshi Fujino, Katsushi Yamaguchi, Toshiyuki T. Yokoyama, Toshiya Hamanaka, Yoritaka Harazono, Hiroaki Kamada, Wataru Kobayashi, Tokuko Ujino-Ihara, Kentaro Uchiyama, Asako Matsumoto,
et al.
with 200 µL of
Lysis Buffer
of the
Maxwell RSC Plant RNA Kit
(
Promega
). RNA was then extracted using a
Maxwell RSC Instrument
(
Promega
) according to the manufacturer’s instructions. The extracted RNA was sequenced by Macrogen
(215)
Single Laboratory Evaluation of the Q20+ Nanopore Sequencing Kit for Bacterial Outbreak Investigations
International Journal of Molecular Sciences
November 5, 2024
Maria Hoffmann, Jay Hee Jang, Sandra M. Tallent, Narjol Gonzalez-Escalona
Measurement Genomic DNA from each
strain
was extracted using either the Maxwell RSC
Cultured Cell
DNA Kit with a
Maxwell RSC Instrument
(
Promega Corporation
, Madison, WI, USA) according to the manufacturer’s instructions for
Gram-negative bacteria
(216)
DNA methylation biomarker panels for differentiating various liver adenocarcinomas, including hepatocellular carcinoma, cholangiocarcinoma, colorectal liver metastases and pancreatic adenocarcinoma liver metastases
Clinical Epigenetics
November 4, 2024
Tina Draškovič, Branislava Ranković, Nina Zidar, Nina Hauptman
isolation was performed using the commercially available
Maxwell RSC FFPE Plus DNA Kit
(
Promega
) on the
Maxwell® RSC Instrument
(
Promega
) according to the manufacturer's instructions. Quantification and quality of DNA isolates were
(217)
High protection and transmission-blocking immunity elicited by single-cycle SARS-CoV-2 vaccine in hamsters
NPJ vaccines
October 30, 2024
Lett MJ, Otte F, Hauser D, Schön J, Kipfer ET, Hoffmann D, Halwe NJ, Breithaupt A, Ulrich L, Britzke T,
et al
.
sequencing
of viral stocks Viral RNA was extracted using the automated Promega Maxwell RSC system (
Promega
,
AS4500
) using either the
Maxwell® RSC Viral Total Nucleic Acid Purification Kit
(
Promega
,
AS1330
) or the Maxwell®
(218)
Gut Bacteria Metabolize Natural and Synthetic Steroid Hormones via the Reductive OsrABC Pathway
bioRxiv
October 19, 2024
Christian Jacoby, Kaylie Scorza, Lia Ecker, Mary McMillin, Ramanujam Ramaswamy, Anitha Sundararajan, Ashley M. Sidebottom, Huaiying Lin, Keith Dufault-Thompson, Brantley Hall,
et al.
subsequent processing. Cell pellets were thawed and total RNA from biological replicates extracted using the
Maxwell RSC instrument
(
Promega
). Extracted RNA was quantified using Qubit, and integrity was measured using TapeStation (Agilent
(219)
Tenacibaculum maritimum can boost inflammation in Dicentrarchus labrax upon peritoneal injection but cannot trigger tenacibaculosis disease
Frontiers in Immunology
October 14, 2024
Inês A. Ferreira, Paulo Santos, Javier Sanz Moxó, Carla Teixeira, Ana do Vale, Benjamin Costas
µL of lysis buffer to the samples, all total RNA isolations were performed by Maxwell® RSC (Cat. #
AS4500
). RNA samples were quantified, and purity was evaluated by
spectrophotometry
using
DeNovix
DS-11 FX
(220)
Tumour-informed liquid biopsies to monitor advanced melanoma patients under immune checkpoint inhibition
Nature Communications
October 9, 2024
Christopher Schroeder, Sergios Gatidis, Olga Kelemen, Leon Schütz, Irina Bonzheim, Francesc Muyas, Peter Martus, Jakob Admard, Sorin Armeanu-Ebinger, Brigitte Gückel,
et al.
paraffin sections depending on tumour size. DNA was extracted using the
Maxwell RSC DNA FFPE Kit
and the
Maxwell RSC Instrument
(
Promega
, Madison, WI, USA) according to the manufacturer’s instructions. There was no pre-defined threshold
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Maxwell RSC Instrument from Promega
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