DeadEnd Fluorometric TUNEL System G3250 from Promega

Product Specs
ItemDeadEnd Fluorometric TUNEL System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberG3250
Quantity60 reactions

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Cerebral glucagon-like peptide-1 receptor activation alleviates traumatic brain injury by glymphatic system regulation in miceCNS neuroscience & therapeuticsDecember 1, 2023Lv C, Han S, Sha Z, Liu M, Dong S, Zhang C, Li Z, Zhang K, Lu S, Xu Z, et al.software. 2.12 TUNEL staining Apoptotic cells were detected by TUNEL assay using the TUNEL assay kit (G3250, Promega) according to the manufacturer's instructions. Cortical neurons were labeled using NeuN immunofluorescence

(2) Impaired lipophagy induced-microglial lipid droplets accumulation contributes to the buildup of TREM1 in diabetes-associated cognitive impairmentAutophagyOctober 1, 2023Li Q, Zhao Y, Guo H, Li Q, Yan C, Li Y, He S, Wang N, Wang Q.quantitative analysis. TUNEL assay To detect neuronal apoptosis in the brain, TUNEL assay (Promega, G3250) was performed on brain sections according to the manufacturer’s instructions. The stained sections

(3) Helicase-like transcription factor (Hltf)-deletion activates Hmgb1-Rage axis and granzyme A-mediated killing of pancreatic β cells resulting in neonatal lethalityPLoS OneAugust 25, 2023Gurvinder Kaur, Rebecca A. Helmer, Dalia Martinez-Marin, Souad R. Sennoune, Rachel L. Washburn, Raul Martinez-Zaguilan, Jannette M. Dufour, Beverly S. Chiltonantifade reagent with DAPI (P36935). The DeadEndTM Fluorometric TUNEL assay (Apoptosis Detection System, G3250) was from Promega Corporation (Madison, WI). 10X Genomics (Pleasanton, CA) was the source of all Visium

(4) MAP4Ks inhibition promotes retinal neuron regeneration from Müller glia in adult miceNPJ Regenerative medicineJuly 13, 2023Zhang H, Guo Y, Yang Y, Wang Y, Zhang Y, Zhuang J, Zhang Y, Shen M, Zhao J, Zhang R, et al.methods. TUNEL Assay TUNEL assay was performed using DeadEnd Fluorometric TUNEL System (Promega, G3250). Frozen sections of organs were rehydrated and incubated with Proteinase K in Tris/HCl (pH = 7.4,10

(5) IGFBP1 Sustains Cell Survival during Spatially-Confined Migration and Promotes Tumor MetastasisAdvanced science (Weinheim, Baden-Wurttemberg, Germany)July 1, 2023Cai G, Qi Y, Wei P, Gao H, Xu C, Zhao Y, Qu X, Yao F, Yang W.cells in lungs were examined by TUNEL staining using the DeadEnd Fluorometric TUNEL System (Promega, G3250) following the manufacturer's protocol. Immunohistochemical Analysis Paraffin‐embedded tissue sections

(6) Transient neurogenesis in ischemic cortex from Sox2(+) astrocytesNeural regeneration researchJuly 1, 2023Yang JL, Fan H, Fu FF, Guo BL, Huang Y, Sun L, Wang WT, Xing JL, Hu XT, Ding YQ, et al.were incubated for 3 hours at room temperature. To perform TUNEL staining, DeadEND™ TUNEL system (Cat#G3250, Promega, Madison, WI, USA) was used according to the product manual. For the combined staining of TUNEL

(7) Hypothalamic JNK1-hepatic fatty acid synthase axis mediates a metabolic rewiring that prevents hepatic steatosis in male mice treated with olanzapine via intraperitoneal: Additional effects of PTP1B inhibitionRedox BiologyJuly 1, 2023Vitor Ferreira, Cintia Folgueira, María García-Altares, Maria Guillén, Mónica Ruíz-Rosario, Giada DiNunzio, Irma Garcia-Martinez, Rosa Alen, Christoph Bookmeyer, John G. Jones, et al.detection of cell death induced by the treatment with OLA by using the DeadEnd™ Fluorometric TUNEL System (G3250, Promega, Madison, WI, USA) according to supplier's instructions. Briefly, tissue sections (5 μm) were

(8) Astrocyte-oligodendrocyte interaction regulates central nervous system regenerationNature communicationsJune 8, 2023Molina-Gonzalez I, Holloway RK, Jiwaji Z, Dando O, Kent SA, Emelianova K, Lloyd AF, Forbes LH, Mahmood A, Skripuletz T, et al.a Tunel assay was performed prior to staining according to the manufacturer’s instructions (Promega G3250). Ex vivo organotypic mouse brain explants Following terminal anaesthesia of P0-P2 CD-1 mouse pups

(9) Characterization of chimeric antigen receptor modified T cells expressing scFv-IL-13Rα2 after radiolabeling with (89)Zirconium oxine for PET imagingJournal of translational medicineJune 7, 2023Leland P, Kumar D, Nimmagadda S, Bauer SR, Puri RK, Joshi BH.performed the assay using DeadEnd fluorometric TUNEL system as per manufacturer’s instructions (Cat# G3250, Promega Corporation, Madison, WI). After a brief wash with 1X PBS, 4 fields in each well were counted

(10) Myoglobin-derived iron causes wound enlargement and impaired regeneration in pressure injuries of muscleeLifeJune 2, 2023Nasir NJM, Heemskerk H, Jenkins J, Hamadee NH, Bunte R, Tucker-Kellogg L.saline-treated mPI and DFO-treated mPI (8 μm sections) were stained with the DeadEnd Fluorometric TUNEL System (G3250; Promega, WI) according to the manufacturer’s protocol. DAPI was used for nuclear staining. Images were

(11) Conserved transcriptional connectivity of regulatory T cells in the tumor microenvironment informs new combination cancer therapy strategiesNature ImmunologyJune 1, 2023Ariella Glasner, Samuel A. Rose, Roshan Sharma, Herman Gudjonson, Tinyi Chu, Jesse A. Green, Sham Rampersaud, Izabella K. Valdez, Emma S. Andretta, Bahawar S. Dhillon, et al.were processed under standardized conditions using the DeadEnd Fluorometric Detection System (Promega, G3250), and subsequent immunohistochemistry was carried out using BOND Polymer Refine Detection Kit (Leica,

(12) Srs11‐92, a ferrostatin‐1 analog, improves oxidative stress and neuroinflammation via Nrf2 signal following cerebral ischemia/reperfusion injuryCNS Neuroscience & TherapeuticsJune 1, 2023Yuhua Chen, Wei He, Hong Wei, Cuicui Chang, Lingjian Yang, Jiao Meng, Tianlin Long, Quanhua Xu, Chi Zhangthe sections were dehydrated and mounted. For TUNEL assay, a DeadEnd™ fluorometric TUNEL system (Cat: G3250; Promega, Madison, WI, USA) was used according to the manufacturer's directions, followed by incubation

(13) RNA m6A-Regulated circ-ZNF609 Suppression Ameliorates Doxorubicin-Induced Cardiotoxicity by Upregulating FTOJACC: Basic to Translational ScienceJune 1, 2023Pujiao Yu, Jiaqi Wang, Gui-e Xu, Xuan Zhao, Xinxin Cui, Jingyi Feng, Jiangpeng Sun, Tianhui Wang, Michail Spanos, Helge Immo Lehmann, et al.(TUNEL) staining in cardiac frozen sections and NRCMs was performed using a commercial kit (Promega, #G3250). Briefly, cardiac sections or NRCMs were fixed with 4% paraformaldehyde for 15 minutes and permeabilized

(14) Self-assembled peptide-substance P hydrogels alleviate inflammation and ameliorate the cartilage regeneration in knee osteoarthritisBiomaterials researchMay 4, 2023Kim SJ, Kim JE, Choe G, Song DH, Kim SJ, Kim TH, Yoo J, Kim SH, Jung Y.TUNEL assay Apoptosis in the cartilage of the knee joint was detected using the DeadEnd™ fluorometric TUNEL system (G3250, Promega, Madison, WI, USA) according to the manufacturer’s instructions. Stained specimens (n = 3 in

(15) Critically short telomeres derepress retrotransposons to promote genome instability in embryonic stem cellsCell DiscoveryMay 2, 2023Nannan Zhao, Guoxing Yin, Chun Liu, Weiyu Zhang, Yang Shen, Dan Wang, Zhenzhen Lin, Jiao Yang, Jian Mao, Renpeng Guo, et al.ends with the terminal deoxynucleotidyl transferase recombinant enzyme (rTdT) using a commercial kit (G3250, Promega). Briefly, after being fixed in 4% paraformaldehyde, washed in PBS, and permeabilized in 0.2%

(16) Pharmacological inhibition of the acetyltransferase Tip60 mitigates myocardial infarction injuryDisease Models & MechanismsMay 1, 2023Xinrui Wang, Tina C. Wan, Katherine R. Kulik, Amelia Lauth, Brian C. Smith, John W. Lough, John A. Auchampachdescribed below. TUNEL TUNEL staining was assessed using a DeadEnd Fluorometric TUNEL System (Promega, #G3250) as per the manufacturer's instructions. The total number of TUNEL-positive cells within sections representing

(17) Microglia activation in the presence of intact blood–brain barrier and disruption of hippocampal neurogenesis via IL-6 and IL-18 mediate early diffuse neuropsychiatric lupusAnnals of the Rheumatic DiseasesMay 1, 2023Dionysis Nikolopoulos, Theodora Manolakou, Alexia Polissidis, Anastasia Filia, George Bertsias, Yassemi Koutmani, Dimitrios T Boumpas4% PFA in PBS for 10 min. TUNEL assay was performed as described by the manufacturer (Promega-CatNo#G3250). For proliferation assays, cells permeabilised with 0.25% Triton X-100 in PBS followed by blocking

(18) A Rational Design of Metal-Organic Framework Nanozyme with High-Performance Copper Active Centers for Alleviating Chemical Corneal BurnsNano-micro lettersApril 30, 2023Tang Y, Han Y, Zhao J, Lv Y, Fan C, Zheng L, Zhang Z, Liu Z, Li C, Lin Y.different formulations for 6 h. Subsequently, the cells were fixed, and the TUNEL staining (catalog no. G3250; Promega, Madison, WI, USA) was performed per the manufacturer's instructions. The coverslips were last

(19) Differential Expression of NME4 in Trophoblast Stem-Like Cells and Peripheral Blood Mononuclear Cells of Normal Pregnancy and PreeclampsiaJournal of Korean medical scienceApril 24, 2023Yu JH, Jung YJ, Kim MS, Cho SR, Kim YH.(TUNEL) assay The levels of cell death in PE were evaluated using a clinical recombinant TUNEL kit (G3250; Promega, Madison, WI, USA). TSLCs from iPSCs were fixed in fresh 4% paraformaldehyde, permeabilized

(20) Zeb1 facilitates corneal epithelial wound healing by maintaining corneal epithelial cell viability and mobilityCommunications BiologyApril 20, 2023Yingnan Zhang, Khoi K. Do, Fuhua Wang, Xiaoqin Lu, John Y. Liu, Chi Li, Brian P. Ceresa, Lijun Zhang, Douglas C. Dean, Yongqing Liuprepared and the TUNEL assays were performed using the DeadEnd Fluorometric TUNEL System (Promega, Cat. # G3250) according to the manufacturer’s instruction. Statistics and reproducibility Student’s t-tests were

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8/11/2015Detecting and Quantifying Apoptotic Cells using DeadEnd™ Fluorometric TUNEL System Lenin VeeravalThis kit was used to detect and quantify the apoptotic cells in a cell population treated with anticancer compounds compared to control cells. The product is simple to use and has high throughput.

DeadEnd Fluorometric TUNEL System

DeadEnd Fluorometric TUNEL System from Promega

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DeadEnd Fluorometric TUNEL System from Promega

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