DeadEnd Fluorometric TUNEL System G3250 from Promega

Product Specs
ItemDeadEnd Fluorometric TUNEL System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberG3250
Quantity60 reactions

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Protective Effect of Brain Derived Neurotrophic Factor-Overexpressing Wharton's Jelly-Derived Mesenchymal Stromal Cells in Severe Intraventricular Hemorrhage in Newborn RatsInternational Journal of Stem CellsFebruary 28, 2028So Yeon Jung, Misun Yang, Young Eun Kim, Dong Kyung Sung, Se In Sung, Chang-Woo Lee, Yun Sil Chang, So Yoon Ahnfluorescence following the manufacturer’s instructions for the DeadEnd Fluorometric TUNEL System kit (G3250; Promega) and counterstained with DAPI. TUNEL-positive cells were observed under a confocal laser scanning

(2) Surface-engineered milk-derived extracellular vesicles enable oral miRNA therapy against immunosuppressive pancreatic cancerMaterials Today BioOctober 1, 2026Youngri Ryu, Hochung Jang, Eun Hye Kim, Junha Lee, Byeongmin Park, Yewon Cho, Wonjun Kim, Hong-Yeol Yoon, Sun Hwa Kim, Dae-Hyuk Kweon, et al.RT. A fluorescent terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay (Promega, #G3250) was conducted according to the manufacturer's protocol to identify apoptotic cells in tumor tissues.

(3) Alpha-N-acetylglucosaminidase (NAGLU) inactivation causes morphological damage to the ultrastructural appearance of epididymal mononuclear phagocytes (eMPs) and epithelial cells of the epididymis in adult micePLoS OneSeptember 25, 2026Lorena Carvelli, Louis Hermo, Daniel G. Cyr, Mary Gregory, Alexey V. Pshezhetsky, Carlos R. Moraleslabeling was performed on paraffin sections using the DeadEnd™ Fluorometric TUNEL System (Promega, Cat# G3250), according to the manufacturer’s instructions, followed by DAPI counterstaining. Fluorescence images

(4) High‑fat diet exacerbates hepatic and adipose insulin signaling impairment and ovarian lesions in a rat model of letrozole‑induced polycystic ovary syndromeMol Med RepSeptember 15, 2026Sunju So, Sujin Kwon, La Yoon Choi, Dae Yong Kim, Mi Hye Kimanalyzed and averaged. TUNEL assay Apoptosis was assessed using the DeadEnd™ Fluorometric TUNEL System (G3250, Promega, WI, USA). Pancreatic sections were deparaffinized with xylene, rehydrated with ethanol, and

(5) Piezo channels contribute to the terminal cytodifferentiation of odontoblasts via modulation of canonical Wnt signalingInt J Oral SciSeptember 10, 2026Xuguang Nie, Kelley Huang, Emily Chu, Man-Kyo ChungDeadEnd Fluorometric TUNEL System, following the manufacturer’s protocol (Promega, Madison, WI, USA, G3250). Tooth germ ex vivo cultures Embryos at the E14.5 stage were collected from timely pregnant mice.

(6) Fibroblast growth factor receptor signaling in maturing osteoblasts controls cell-matrix interactions critical for osteocyte survivalBone ResSeptember 7, 2026Debabrata Patra, Craig Smith, Chenming Wei, Courtney M. Mazur, Ilemona Ameadaji, Tiandao Li, Marc N. Wein, Matthew J. Silva, David M. Ornitzwere performed on FFPE sections using the DeadEndTM Fluorometric TUNEL System staining kit (Promega, G3250) and manufacturer’s instructions provided. Images of stained bone sections were captured at high resolution

(7) Redox imbalance dictates dependence on GOT1 versus GOT2 for rod photoreceptor health during aging and stressRedox BiologySeptember 1, 2026Meini Chen, Eric Weh, Moloy T. Goswami, Katherine M. Weh, Heather Hager, Peter Sajjakulnukit, Avi J. Weingarten, Shubha Subramanya, Nicholas Miller, Sraboni Chaudhury, et al.were washed and resuspended before staining using the DeadEnd Fluorometric TUNEL kit (Promega, Cat #G3250). Stained samples were analyzed using an Attune NXT flow cytometer (Thermo Fisher Scientific; FITC channel

(8) ALY688 Protects Against Myocardial Ischemia-Reperfusion Injury via Direct Effects and Rab8a-Dependent Extracellular VesiclesJ Extracell VesiclesAugust 29, 2026Hye Kyoung Sung, Jialing Tang, Yubin Lei, Khang Nguyen, Eddie Tam, Jun Wu, Ren-Ke Li, Vincent Richard, Christoph H. Borchers, Lina Antounians, et al.sections were subjected to H&E (Sigma, HT1079, HT110216), Masson Trichrome (Sigma, HT15), TUNEL (Promega, G3250) according to manufacturer's protocol and visualized using a bright field slide scanning microscopy

(9) Klotho-Derived Peptide 1 Protects against Acute Kidney Injury by Directly Targeting Mitochondrial ATAD3AAdv Sci (Weinh)August 25, 2026Xiaoyao Zhang, Shihui Lin, Tianyu Wu, Zhixin Zhang, Hong Zhou, Xue Hong, Youhua Liu(PAS) staining was performed to evaluate the extent of renal tubular injury, while TUNEL staining (G3250; Promega, Madison, WI) was employed to quantify apoptosis. After antigen unmasking, sections were incubated

(10) Hypertensive mt. tRNAIle4263A>G mutation orchestrates vascular senescence and apoptosis by activation of mitochondria-ER interplayRedox BiologyAugust 20, 2026Nian Cao, Haijing Zhao, Zhengfeng Wu, Shuagnlei Li, Wei Tong, Lihao Jin, Ming Wu, Jie Liu, Aihong Liu, Meng Wang, et al.staining. TdT-mediated dUTP Nick-End Labeling staining was assessed using a commercial kit (Promega, G3250) following the manufacturer's protocol. Briefly, 6-μm frozen sections of VOs were permeabilized with

(11) Neurodevelopmental outcomes relevant to autism in juvenile mice exposed to PCB 11 in the maternal diet throughout gestation and lactationFront ToxicolAugust 12, 2026Rebecca J. Wilson, Harmanpreet K. Panesar, Ilknur Dursun, Rosalia Mendieta, Peter M. Andrew, Xueshu Li, Hans-Joachim Lehmler, Pamela J. Leinstaining TUNEL staining was performed using the DeadEnd Fluorometric TUNEL System (Promega, catalog # G3250; Madison, WI, United States) according to the manufacturer’s instructions using solutions provided in

(12) TSPO Modulation by PIGA-1138 Attenuates Oxidative Stress and Preserves Retinal Function in Experimental Diabetic RetinopathyAntioxidants (Basel)August 12, 2026Alessia Galante, Francesca Corsi, Rosario Amato, Sabrina Taliani, Federico Da Settimo, Maurizio Cammalleri, Ilaria Piano, Massimo Dal Monte, Claudia Garginifor 5 min. Nuclear DNA fragmentation was assessed using the DeadEnd™ Fluorometric TUNEL System (cat #G3250, Promega, Madison, WI, USA), following the manufacturer’s instructions. Nuclei were counterstained with

(13) Targeting CD44 reverses sphingomyelin-induced oligodendrocyte maturation arrest in acid sphingomyelinase deficiencyEMBO Molecular MedicineAugust 12, 2026Marta Guerrero-Valero, Elena Melgarejo, Miguel A Marchena, Beatriz Fernández-Gómez, Jaime Mulero-Franco, Fernando de Castro, Edward H Schuchman, María Dolores Ledesmaprocessed using the DeadEnd™ Fluorometric TUNEL (TdT-mediated dUTP Nick-End Labeling) System (Promega; # G3250). In vivo analyses of OL and myelin The OL lineage was characterized using stage-specific markers,

(14) Genome-Wide Identification of the SRZ Gene Family in Rice and Arabidopsis, and Functional Analysis of OsSRZ1 in Regulating Panicle ExsertionBiology (Basel)August 8, 2026Chao Zhang, Sitian Fei, Yingxiang Hou, Jingjing Xu, Qiannan Sun, Chaofeng Shen, Siyu Liu, Lan Li, Yong Luoparaffin wax [38]. TUNEL labeling was performed using a DeadEnd Fluorometric TUNEL system (Promega, #G3250, Madison, WI, USA) according to the manufacturer’s instructions. 3. Results 3.1. Identification of

(15) Cross-species functional analysis of a de novo DCLK1 variant associated with a neurodevelopmental disorderResearchSquareJuly 23, 2026David F. Butler, Weimin Butler, Hirokazu Hashimoto, Hirokazu Hashimoto, Jonathan H. Sheehan, Jonathan Sheehan, Vanessa Rosenfeld, Lindsay C. Rosenfeld, Lindsay Silverman, Patricia Silverman, et al.assay Apoptotic cells were detected using the Promega DeadEnd™ Fluorometric TUNEL System (Promega Product # G3250) based on the manufacturer’s protocol. Following fixation, cells with permeabilized 0.2% Triton X-100

(16) Long non-coding RNA LOC101926915-encoded micropeptide carboplatin-induced upregulation factor confers carboplatin resistance in ovarian cancerCytojournalJuly 21, 2026Man Li, YingRu Xu, ZhiBiao Mai, Liangliang Ren, Xuexin Yu, SuWen Chen, ZunFu Ke, Ruyuan Yulabeling (TUNEL) assay Apoptotic cells were identified with the DeadEnd ™ Fluorometric TUNEL System (Cat# G3250, Promega, Madison, WI, USA) following the protocol provided by the manufacturer. For paraffin-embedded

(17) Surface-cleaned black phosphorus nanosheets chelate iron and rebalance the ferroptosis-immune axis in acute kidney injuryTheranosticsJuly 20, 2026Wan-Lin Tan, Jin-Ming Zhu, Xiao-Xiao Xu, Jing Jia, Rong-Yu Chen, Xiong Yu, Lu-Qun Liang, Yuan-Yuan Ruan, Fang-Fang Wang, Yu-Ting Chen, et al.terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) Apoptosis Assay Kit (Promega, G3250, USA). After deparaffinization, sections were incubated with 20 μg/mL proteinase K for 15 min at room

(18) Pharmacological modulation of p75 neurotrophin receptor in microglial cells improves resilience to rotenone cytotoxicityFront PharmacolJuly 20, 2026Alessio Valenza, Daniele Pensabene, Francesca Rendina, Maurizio Muzzi, Anna Fracassi, Sandra Moreno, Marco Segattosecondary-electron detection at 5 kV. 2.6 TUNEL assay Detection of apoptotic cells by TUNEL assay (G3250, Promega) was performed as previously reported (Marioli et al., 2024). Following experimental treatments,

(19) GenX exposure induces neurodevelopmental impairment and synaptic toxicity in hESC-derived cerebral organoidsMater Today BioJuly 15, 2026Sung-Ae Hyun, Young-Ju Lee, Moon Yi Ko, Euijun Min, Heejin Park, Younhee Kim, Dae Youn Hwang, Byoung-Seok Lee, Minhan Kadeath in day 20 cerebral organoids was assessed using the DeadEnd™ Fluorometric TUNEL System (Promega, G3250) according to the manufacturer's instructions. Briefly, organoid sections were fixed in 4% formaldehyde

(20) Saccharomyces boulardii CNCM I-745 protects against small intestinal injury in a model of gliadin-peptide-induced sterile inflammatory enteropathyFront ImmunolJuly 6, 2026Aluminé Lezcano, Emanuel Miculan, Geronimo Ducca, Carolina Ruera, María Luz Iribarren, Philippe Tixador, Xavier Roux, Fernando Gabriel Chirdodeparaffinization and rehydration, the sections were treated with the DeadEnd™ Fluorometric TUNEL System (Cat# G3250, Promega) according to the manufacturer’s instructions. Nuclear staining was performed with propidium

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8/11/2015Detecting and Quantifying Apoptotic Cells using DeadEnd™ Fluorometric TUNEL System Lenin VeeravalThis kit was used to detect and quantify the apoptotic cells in a cell population treated with anticancer compounds compared to control cells. The product is simple to use and has high throughput.

DeadEnd Fluorometric TUNEL System

DeadEnd Fluorometric TUNEL System from Promega

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DeadEnd Fluorometric TUNEL System from Promega

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