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Assay Kit
DeadEnd Fluorometric TUNEL System
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DeadEnd Fluorometric TUNEL System
DeadEnd Fluorometric TUNEL System
DeadEnd Fluorometric TUNEL System from Promega
(1)
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Citations:
(645)
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DeadEnd Fluorometric TUNEL System
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Description
DNA fragmentation detection assay. Designed for the detection and quantitation of apoptotic cells within a cell population
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
DeadEnd Fluorometric TUNEL System
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
G3250
Quantity
60 reactions
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Promega
2800 Woods Hollow Rd.
Madison
,
Wisconsin
United States
Phone
: 800-356-9526
Fax: 800-356-1970
Company Profile
Website:
http://www.promega.com
Citations (645)
(41)
RNA m6A-Regulated circ-ZNF609 Suppression Ameliorates Doxorubicin-Induced Cardiotoxicity by Upregulating FTO
JACC: Basic to Translational Science
June 1, 2023
Pujiao Yu, Jiaqi Wang, Gui-e Xu, Xuan Zhao, Xinxin Cui, Jingyi Feng, Jiangpeng Sun, Tianhui Wang, Michail Spanos, Helge Immo Lehmann,
et al.
(
TUNEL
)
staining
in cardiac
frozen sections
and NRCMs was performed using a commercial kit (
Promega
, #
G3250
). Briefly, cardiac sections or NRCMs were fixed with 4% paraformaldehyde for 15 minutes and permeabilized
(42)
A combination of genetically engineered oncolytic virus and melittin-CpG for cancer viro-chemo-immunotherapy
BMC Medicine
May 24, 2023
Amirhossein Bahreyni, Huitao Liu, Yasir Mohamud, Yuan Chao Xue, Yiyun Michelle Fan, Yizhuo Lyanne Zhang, Honglin Luo
tissues (collected at humane endpoints) to assess apoptosis according to the manufacturer’s protocol (
G3250
,
Promega
). Safety analysis To assess the safety of each treatment, the
body weight
of
mice
in each
(43)
Forward genetic screening using fundus spot scale identifies an essential role for Lipe in murine retinal homeostasis
Communications Biology
May 17, 2023
Seher Yuksel, Bogale Aredo, Yeshumenesh Zegeye, Cynthia X. Zhao, Miao Tang, Xiaohong Li, John D. Hulleman, Laurent Gautron, Sara Ludwig, Eva M. Y. Moresco,
et al.
of first report65 and literature supplied with the
DeadEnd Fluorometric TUNEL System
(
Promega
Cat #
G3250
). Sections subjected to
TUNEL
were counterstained with propidium iodide. To check for the presence or
(44)
Self-assembled peptide-substance P hydrogels alleviate inflammation and ameliorate the cartilage regeneration in knee osteoarthritis
Biomaterials research
May 4, 2023
Kim SJ, Kim JE, Choe G, Song DH, Kim SJ, Kim TH, Yoo J, Kim SH, Jung Y.
TUNEL assay
Apoptosis in the
cartilage
of the knee joint was detected using the
DeadEnd™ fluorometric TUNEL system
(
G3250
,
Promega
, Madison, WI, USA) according to the manufacturer’s instructions. Stained specimens (n = 3 in
(45)
Critically short telomeres derepress retrotransposons to promote genome instability in embryonic stem cells
Cell Discovery
May 2, 2023
Nannan Zhao, Guoxing Yin, Chun Liu, Weiyu Zhang, Yang Shen, Dan Wang, Zhenzhen Lin, Jiao Yang, Jian Mao, Renpeng Guo,
et al.
ends with the terminal deoxynucleotidyl transferase recombinant enzyme (rTdT) using a commercial kit (
G3250
,
Promega
). Briefly, after being fixed in 4% paraformaldehyde, washed in PBS, and permeabilized in 0.2%
(46)
Microglia activation in the presence of intact blood–brain barrier and disruption of hippocampal neurogenesis via IL-6 and IL-18 mediate early diffuse neuropsychiatric lupus
Annals of the Rheumatic Diseases
May 1, 2023
Dionysis Nikolopoulos, Theodora Manolakou, Alexia Polissidis, Anastasia Filia, George Bertsias, Yassemi Koutmani, Dimitrios T Boumpas
4% PFA in PBS for 10 min.
TUNEL assay
was performed as described by the manufacturer (
Promega
-CatNo#
G3250
). For proliferation
assays
, cells permeabilised with 0.25% Triton X-100 in PBS followed by blocking
(47)
Pharmacological inhibition of the acetyltransferase Tip60 mitigates myocardial infarction injury
Disease Models & Mechanisms
May 1, 2023
Xinrui Wang, Tina C. Wan, Katherine R. Kulik, Amelia Lauth, Brian C. Smith, John W. Lough, John A. Auchampach
described below.
TUNEL
TUNEL
staining
was assessed using a
DeadEnd Fluorometric TUNEL System
(
Promega
, #
G3250
) as per the manufacturer's instructions. The total number of
TUNEL
-positive cells within sections representing
(48)
A Rational Design of Metal-Organic Framework Nanozyme with High-Performance Copper Active Centers for Alleviating Chemical Corneal Burns
Nano-micro letters
April 30, 2023
Tang Y, Han Y, Zhao J, Lv Y, Fan C, Zheng L, Zhang Z, Liu Z, Li C, Lin Y.
different formulations for 6 h. Subsequently, the cells were fixed, and the
TUNEL
staining
(catalog no.
G3250
;
Promega
, Madison, WI, USA) was performed per the manufacturer's instructions. The coverslips were last
(49)
Differential Expression of NME4 in Trophoblast Stem-Like Cells and Peripheral Blood Mononuclear Cells of Normal Pregnancy and Preeclampsia
Journal of Korean medical science
April 24, 2023
Yu JH, Jung YJ, Kim MS, Cho SR, Kim YH.
(
TUNEL) assay
The levels of cell death in PE were evaluated using a clinical recombinant
TUNEL
kit (
G3250
;
Promega
, Madison, WI, USA). TSLCs from iPSCs were fixed in fresh 4% paraformaldehyde, permeabilized
(50)
Zeb1 facilitates corneal epithelial wound healing by maintaining corneal epithelial cell viability and mobility
Communications Biology
April 20, 2023
Yingnan Zhang, Khoi K. Do, Fuhua Wang, Xiaoqin Lu, John Y. Liu, Chi Li, Brian P. Ceresa, Lijun Zhang, Douglas C. Dean, Yongqing Liu
prepared and the
TUNEL
assays
were performed using the
DeadEnd Fluorometric TUNEL System
(
Promega
, Cat. #
G3250
) according to the manufacturer’s instruction. Statistics and reproducibility Student’s t-tests were
(51)
Evidence of Spaceflight-Induced Adverse Effects on Photoreceptors and Retinal Function in the Mouse Eye
International Journal of Molecular Sciences
April 17, 2023
Xiaowen Mao, Seta Stanbouly, Jacob Holley, Michael Pecaut, James Crapo
paraffin-embedded sections were processed using
DeadEnd™ Fluorometric TUNEL system
kit (catalog no.
G3250
,
Promega
Corp., Madison, WI, USA). The same sections were then incubated with DyLight 594 Lycopersicon
(52)
Sphingomyelin 16:0 is a therapeutic target for neuronal death in acid sphingomyelinase deficiency
Cell death & disease
April 6, 2023
Gaudioso Á, Jiang X, Casas J, Schuchman EH, Ledesma MD.
determined using DeadEnd™ Fluorometric
TUNEL
(TdT-mediated
dUTP
Nick-End
Labeling
) System (
Promega
; #
G3250
) following the manufacturer instructions. Propargyl SM Cultured
neurons
were treated with 40 µM propargyl
(53)
Serine-arginine protein kinase 1 (SRPK1) promotes EGFR-TKI resistance by enhancing GSK3β Ser9 autophosphorylation independent of its kinase activity in non-small-cell lung cancer
Oncogene
April 6, 2023
Jing-Qiang Huang, Ling-Xin Duan, Qiu-Yu Liu, He-Feng Li, Ao-Ping Hu, Jun-Wei Song, Chuxuan Lin, Bingsheng Huang, Da Yao, Bin Peng,
et al.
for 2 min. The cells were then washed with PBS and incubated with 50 µL
TUNEL
reaction mixture (cat:
G3250
;
Promega
, USA) containing the rTdT enzyme for 1 h at 37°C. After washing three times with PBS, the cells
(54)
SCAP contributes to embryonic angiogenesis by negatively regulating KISS-1 expression in mice
Cell death & disease
April 6, 2023
Zheng G, Su Y, Wei L, Yao Y, Wang Y, Luo X, Wang X, Ruan XZ, Li D, Chen Y.
ImageJ software.
TUNEL
staining
The
assay
was conducted according to the manufacturer’s protocol (
G3250
,
Promega
, Madison, WI, USA). To detect DNA strand breaks occurring in apoptotic cells,
frozen sections
(55)
Asparagine protects pericentral hepatocytes during acute liver injury
Journal of Clinical Investigation
April 3, 2023
Yu Sun, Hadrien Demagny, Adrien Faure, Francesca Pontanari, Antoine Jalil, Nadia Bresciani, Ece Yildiz, Melanie Korbelius, Alessia Perino, Kristina Schoonjans
was incubated for 10 minutes for nuclear
staining
.
TUNEL assay
. A
TUNEL assay
kit (
Promega
, catalog
G3250
) was used to detect DNA damage, according to the manufacturer’s instructions. After
TUNEL
labeling
,
(56)
A stress-induced cilium-to-PML-NB route drives senescence initiation
Nature communications
April 3, 2023
Ma X, Zhang Y, Zhang Y, Zhang X, Huang Y, He K, Chen C, Hao J, Zhao D, LeBrasseur NK,
et al
.
of the cells in senescence was detected with a
TUNEL assay
kit (
DeadEnd™ Fluorometric TUNEL System
,
G3250
,
Promega
) following the manufacturer’s protocol.
In vitro
SUMOylation The in vitro SUMOylation kit
(57)
D-Aspartate Depletion Perturbs Steroidogenesis and Spermatogenesis in Mice
Biomolecules
March 30, 2023
Alessandra Santillo, Sara Falvo, Massimo Venditti, Anna Di Maio, Gabriella Chieffi Baccari, Francesco Errico, Alessandro Usiello, Sergio Minucci, Maria Maddalena Di Fiore
TUNEL Assay
Apoptosis was examined in paraffin sections by the
TUNEL-assay
using
DeadEnd™ Fluorometric TUNEL System
(#
G3250
;
Promega
Corp., Madison, WI, USA) following the manufacturer’s protocol with modifications. Before the
(58)
In vivo RNA-seq and ChIP-seq analyses show an obligatory role for the C terminus of p53 in conferring tissue-specific radiation sensitivity
Cell Reports
March 28, 2023
Lois Resnick-Silverman, Royce Zhou, Moray J. Campbell, Ian Leibling, Ramon Parsons, James J. Manfredi
-
SYBR Green PCR Master Mix
,
Thermo Fisher
-
Applied Biosystems
,
4367659
DeadEnd™ Fluorometric TUNEL System
,
ProMega
,
G3250
RNAeasy Mini Kit,
Qiagen
,
74106
QIA Shredder,
Qiagen
,
79656
QIAquick PCR purification kit,
Qiagen
,
28104
,, Deposited
(59)
An actin filament branching surveillance system regulates cell cycle progression, cytokinesis and primary ciliogenesis
Nature communications
March 27, 2023
Cao M, Zou X, Li C, Lin Z, Wang N, Zou Z, Ye Y, Seemann J, Levine B, Tang Z,
et al
.
staining
was performed on
tumor
xenograft sections according to the manufacturer’s instructions (
Promega
,
G3250
). Clinical
human
specimens
Colon cancer
and
lung cancer
sections, with corresponding normal tissue
(60)
Therapeutic Effects of WT1 Silencing via Respiratory Administration of Neutral DOPC Liposomal-siRNA in a Lung Metastasis Melanoma Murine Model
Non-Coding RNA
March 22, 2023
Martin R. Ramos-Gonzalez, Eduardo Vazquez-Garza, Gerardo Garcia-Rivas, Cristian Rodriguez-Aguayo, Arturo Chavez-Reyes
coverslips were washed with PBS and stained with the kit
DeadEnd Fluorometric TUNEL System
(
Promega
, Cat.
G3250
) following the merchant’s instructions. Lastly, the coverslips were mounted over a slide using
VectaShield with DAPI
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Reviews
Write a Review
(1)
8/11/2015
Detecting and Quantifying Apoptotic Cells using DeadEnd Fluorometric TUNEL System
Lenin Veeraval
This kit was used to detect and quantify the apoptotic cells in a cell population treated with anticancer compounds compared to control cells. The product is simple to use and has high throughput.
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DeadEnd Fluorometric TUNEL System from Promega
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