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Assay Kit
DeadEnd Fluorometric TUNEL System
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DeadEnd Fluorometric TUNEL System
DeadEnd Fluorometric TUNEL System
DeadEnd Fluorometric TUNEL System from Promega
(1)
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Citations:
(645)
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DeadEnd Fluorometric TUNEL System
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Description
DNA fragmentation detection assay. Designed for the detection and quantitation of apoptotic cells within a cell population
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
DeadEnd Fluorometric TUNEL System
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
G3250
Quantity
60 reactions
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Promega
2800 Woods Hollow Rd.
Madison
,
Wisconsin
United States
Phone
: 800-356-9526
Fax: 800-356-1970
Company Profile
Website:
http://www.promega.com
Citations (645)
(61)
Cocktails of NSAIDs and 17α Ethinylestradiol at Environmentally Relevant Doses in Drinking Water Alter Puberty Onset in Mice Intergenerationally
International journal of molecular sciences
March 20, 2023
Philibert P, Déjardin S, Girard M, Durix Q, Gonzalez AA, Mialhe X, Tardat M, Poulat F, Boizet-Bonhoure B.
the Dead-End Fluorometric
TUNEL
Apoptosis Assay
kit according to the supplier’s
procedure
(
Promega
,
G3250
). After mounting in a Fluorsave mounting medium, IF images were captured with a
Zeiss
AxioImager
apotome
(62)
The JAK-STAT pathway promotes persistent viral infection by activating apoptosis in insect vectors
PLOS Pathogens
March 16, 2023
Yan Zhang, Bo-Xue Li, Qian-Zhuo Mao, Ji-Chong Zhuo, Hai-Jian Huang, Jia-Bao Lu, Chuan-Xi Zhang, Jun-Min Li, Jian-Ping Chen, Gang Lu
software. Terminal uridine nick-end
labeling
(
TUNEL) assay
The
DeadEnd Fluorometric TUNEL System
(
G3250
,
Promega
, USA) was used for
TUNEL
staining
. 15
insect
midguts and 15
salivary glands
were dissected
(63)
The simultaneous administration of microplastics and cadmium alters rat testicular activity and changes the expression of PTMA, DAAM1 and PREP
Frontiers in Cell and Developmental Biology
March 9, 2023
Massimo Venditti, Majida Ben Hadj Hassine, Imed Messaoudi, Sergio Minucci
Germany). Apoptotic cells were investigated by
TUNEL-assay
using the
DeadEnd™ Fluorometric TUNEL System
(#
G3250
;
Promega
Corp., Madison, WI, United States) following manufacturer’s protocol. The sections were then
(64)
Ambra1 haploinsufficiency in CD1 mice results in metabolic alterations and exacerbates age-associated retinal degeneration
Autophagy
March 1, 2023
Ramírez-Pardo I, Villarejo-Zori B, Jiménez-Loygorri JI, Sierra-Filardi E, Alonso-Gil S, Mariño G, de la Villa P, Fitze PS, Fuentes JM, García-Escudero R,
et al
.
wet chamber before
DAPI
counterstaining.
TUNEL assay
(
DeadEnd™ Fluorometric TUNEL System
;
Promega
,
G3250
) was used to detect apoptotic cells in
cryosections
following the manufacturer’s directions. Briefly,
(65)
TNF-α contributes to sarcopenia through caspase-8/caspase-3/GSDME-mediated pyroptosis
Cell death discovery
February 24, 2023
Wu J, Lin S, Chen W, Lian G, Wu W, Chen A, Sagor MIH, Luo L, Wang H, Xie L.
terminal deoxynucleotidyl transferase-mediated digoxigenin-deoxyuridine nick-end
labeling
(
TUNEL
) System (
G3250
) was obtained from
Promega
(WI, USA). LDH cytotoxicity assay detection kit was obtained from Beyotime
(66)
Spatio-temporal dynamics enhance cellular diversity, neuronal function and further maturation of human cerebral organoids
Communications Biology
February 14, 2023
Pelin Saglam-Metiner, Utku Devamoglu, Yagmur Filiz, Soheil Akbari, Goze Beceren, Bakiye Goker, Burcu Yaldiz, Sena Yanasik, Cigir Biray Avci, Esra Erdal,
et al.
light microscopy
(Zeiss Axio Vert.A1).
TUNEL
apoptosis test
DeadEnd™ Fluorometric TUNEL System
(
Promega
,
G3250
)
assay
was used to examine apoptotic regions in matured
organoids
. Initially, the paraffin embedded
(67)
Combination treatment of T1-44, a PRMT5 inhibitor with Vactosertib, an inhibitor of TGF-β signaling, inhibits invasion and prolongs survival in a mouse model of pancreatic tumors
Cell death & disease
February 10, 2023
Hong E, Barczak W, Park S, Heo JS, Ooshima A, Munro S, Hong CP, Park J, An H, Park JO,
et al
.
detect the apoptotic
tumor
cells in
tumor
tissues,
DeadEnd™ Fluorometric TUNEL System
(
Promega
, Cat. No.
G3250
) was used with manufacturer’s protocol. Briefly, the
tumor
tissue slides were deparaffinized and rehydrated
(68)
Reprogramming of ovarian aging epigenome by resveratrol
PNAS Nexus
February 3, 2023
Mo Gou, Jie Li, Lizhi Yi, Huiyu Li, Xiaoying Ye, Huasong Wang, Linlin Liu, Baofa Sun, Song Zhang, Zhengmao Zhu,
et al.
tissues or cells was performed strictly in accordance with the instructions provided in the DeadEnd™ kit (
G3250
,
Promega
). Briefly, for tissues, after deparaffinization, the slide was fixed with 4% paraformaldehyde
(69)
lncRNA LINC00960 promotes apoptosis by sponging ubiquitin ligase Nrdp1-targeting miR-183-5p
Acta Biochimica et Biophysica Sinica
January 31, 2023
Hao Yang, Tianxia Jiang, Libin Fan, Xiaobo Qiu
laser at 488 or 594 nm for excitation.
TUNEL assay
Apoptosis was analysed using a
TUNEL assay
kit (
G3250
;
Promega
,Madison, USA) according to the manufacturer’s instructions. Briefly, after growing cells on
(70)
Germ Cell Maintenance and Sustained Testosterone and Precursor Hormone Production in Human Prepubertal Testis Organ Culture with Tissues from Boys 7 Years+ under Conditions from Adult Testicular Tissue
Cells
January 26, 2023
Neels Lennart Aden, Matthias Bleeke, Uwe R. Kordes, Bianka Brunne, Barbara Holstermann, Ronald Biemann, Uta Ceglarek, Armin Soave, Andrea Salzbrunn, Stefan W. Schneider,
et al.
cells/areas in the tissue,
TUNEL
staining
was performed using the DeadEndTM Fluorometric
TUNEL
System (#
G3250
,
Promega
, Madison, WI, USA). For the protocol, rehydrated sections as for
immunofluorescence
staining
,
(71)
A male-sterile mutant with necrosis-like dark spots on anthers was generated in cotton
Frontiers in Plant Science
January 9, 2023
Jun Zhang, Peng Wu, Ning Li, Xiaolan Xu, Songxin Wang, Siyuan Chang, Yuping Zhang, Xingxing Wang, Wangshu Liu, Yizan Ma,
et al.
TUNEL
analysis of the fragmented DNA of apoptotic cells using the
DeadEnd™ Fluorometric TUNEL System
(
G3250
,
Promega
). The analytical wavelengths of fluorescein and propidium iodide were 520 ± 10 nm and 640 ±
(72)
Auditory or Audiovisual Stimulation Ameliorates Cognitive Impairment and Neuropathology in ApoE4 Knock-In Mice
International Journal of Molecular Sciences
January 4, 2023
Harry Jung, Yeonkyeong Lee, Sang-Hwa Lee, Jong-Hee Sohn
(
TUNEL) Assay
Apoptotic cells in the hippocampus and cortex were assessed using the
DeadEnd fluorometric TUNEL system
(
G3250
,
Promega
, Madison, WI, USA) in tissue slices permeabilized in 0.2% Triton X-100 (TX1061, Georgiachem,
(73)
Preclinical investigation of patient-derived cervical cancer organoids for precision medicine
Journal of Gynecologic Oncology
January 1, 2023
Hyang Sook Seol, Ju Hee Oh, Eunhye Choi, SangMin Kim, Hyunki Kim, Eun Ji Nam
radiation treatment
at each dose (0, 2, 6, 12 Gy). Paraffin-embedded sections were treated with
TUNEL
kit reagents (
G3250
;
Promega
) according to the manufacturer’s instructions. After
incubation
with the
staining
reagents,
(74)
Deletion of histone demethylase Lsd1 (Kdm1a) during retinal development leads to defects in retinal function and structure
Frontiers in cellular neuroscience
January 1, 2023
Ferdous S, Shelton DA, Getz TE, Chrenek MA, L'Hernault N, Sellers JT, Summers VR, Iuvone PM, Boss JM, Boatright JH,
et al
.
Table 1.
TUNEL
The manufacturer instructions for the Promega DeadEnd
TUNEL
Fluorometric kit (
Promega
G3250
) were followed. In brief, tissue sections were deparaffinized in 5 steps of xylene for 2 min each. The
(75)
Apoptotic extracellular vesicles are metabolized regulators nurturing the skin and hair
Bioactive materials
January 1, 2023
Ma L, Chen C, Liu D, Huang Z, Li J, Liu H, Kin Kwok RT, Tang B, Sui B, Zhang X,
et al
.
and permeabilized. According to the manufacture's protocol, cells were incubated with
TUNEL
reagent (
G3250
,
Promega
) for 60 min at 37 °C. Positive cells were counted after counterstained with DAPI under microscope. 4.6
(76)
L- and T-type Ca2+ channels dichotomously contribute to retinal ganglion cell injury in experimental glaucoma
Neural Regeneration Research
January 1, 2023
Xing-Huai Wang, Zhong-Feng Wang, Hong-Ning Wang, Wen-Jing Qian, Yan-Ying Zhao, Fang Lei, Xing-Huai Miao, Zhong-Feng Wang
flat-mounted retinas (Dong et al., 2015; Xu et al., 2020) using the
DeadEnd Fluorometric TUNEL System
G3250
kit (
Promega
, Madison, WI, USA). All
TUNEL
-positive RGCs that were merged well with 4′,6-diamidino-2-phenylindole
(77)
K. ZHENG ET AL.Gold-nanoparticle-based multistage drug delivery system for antitumor therapy
Drug Delivery
December 31, 2022
Kaikai Zheng, Dong Zhou, Lili Wu, Jian Li, Bing Zhao, Shihao Zhang, Ruiying He, Lan Xiao, Iqbal Zoya, Li Yu,
et al.
transferase-mediated deoxynucleotide triphosphate nick-end
labeling
(
TUNEL
) bright green
apoptosis detection
kit (
G3250
,
Promega
Biotech Co., Ltd., USA) according to the manufacturer’s instructions. Nuclei were detected
(78)
Protective effect of salidroside on streptozotocin-induced diabetic nephropathy by inhibiting oxidative stress and inflammation in rats via the Akt/GSK-3β signalling pathway
Pharmaceutical Biology
December 31, 2022
Delong Pei, Shengri Tian, Yanqiu Bao, Jun Zhang, Dongyuan Xu, Minhu Piao
Biotechnology Malondialdehyde assay kit,A003-1-2,Jiancheng Biotechnology
DeadEnd Fluorometric TUNEL System
,
G3250
,
Promega
Bcl-2
,
sc-7382
,
Santa Cruz Biotechnology
Bax
,
sc-7480
,
Santa Cruz Biotechnology
pSer473-
Akt
,
sc-514032
,
Santa Cruz Biotechnology
(79)
Chimeric efferocytic receptors improve apoptotic cell clearance and alleviate inflammation
Cell
December 22, 2022
Sho Morioka, Daiki Kajioka, Yusuke Yamaoka, Rochelle M. Ellison, Turan Tufan, Inge L. Werkman, Shinji Tanaka, Brady Barron, Satoshi T. T Ito, Sarah Kucenas,
et al.
Assay
Kit,abcam,ab105134 Plasma creatinine
assay
kit,Diazyme Laboratories,DZ072B
TUNEL assay
,
Promega
,
G3250
Apoptag ISOL dual
fluorescence
apoptosis detection
kit,
Millipore
,
APT1000
Deposited data,Deposited
(80)
Targeting aberrant replication and DNA repair events for treating breast cancers
Communications Biology
December 1, 2022
Subapriya Rajamanickam, Jun Hyoung Park, Panneerdoss Subbarayalu, Santosh Timilsina, Kaitlyn Bates, Pooja Yadav, Saif S. R. Nirzhor, Vijay Eedunuri, Tabrez A. Mohammad, Kwang Hwa Jung,
et al.
antibody against Ki67 (#NB500, Novus,
1:100
) or subjected to
apoptosis assay
using a
TUNEL assay
kit (#
G3250
,
Promega
Inc.) as described previously15. Ki67 and
TUNEL
-positive cells were counted at ten arbitrarily
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Reviews
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(1)
8/11/2015
Detecting and Quantifying Apoptotic Cells using DeadEnd Fluorometric TUNEL System
Lenin Veeraval
This kit was used to detect and quantify the apoptotic cells in a cell population treated with anticancer compounds compared to control cells. The product is simple to use and has high throughput.
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DeadEnd Fluorometric TUNEL System from Promega
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