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DeadEnd Fluorometric TUNEL System
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DeadEnd Fluorometric TUNEL System
DeadEnd Fluorometric TUNEL System
DeadEnd Fluorometric TUNEL System from Promega
(1)
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Citations:
(645)
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DeadEnd Fluorometric TUNEL System
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Description
DNA fragmentation detection assay. Designed for the detection and quantitation of apoptotic cells within a cell population
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
DeadEnd Fluorometric TUNEL System
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
G3250
Quantity
60 reactions
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Promega
2800 Woods Hollow Rd.
Madison
,
Wisconsin
United States
Phone
: 800-356-9526
Fax: 800-356-1970
Company Profile
Website:
http://www.promega.com
Citations (645)
(101)
Therapeutic hypothermia alleviates myocardial ischaemia–reperfusion injury by inhibiting inflammation and fibrosis via the mediation of the SIRT3 / NLRP3 signalling pathway
Journal of Cellular and Molecular Medicine
October 1, 2022
Jing Zhang, Yimei Lu, Peng Yu, Zhangwang Li, Yang Liu, Jun Zhang, Xiaoyi Tang, Shuchun Yu
level were collected for paraffin sectioning. Paraffin sections were stained with the
TUNEL assay
kit (
G3250
,
Promega
, Madison, WI, USA) to examine
cardiomyocyte
apoptosis, according to the manufacturer's instructions.
(102)
Preservation effect of imeglimin on pancreatic β-cell mass: Noninvasive evaluation using 111In-exendin-4 SPECT/CT imaging and the perspective of mitochondrial involvements
Front Endocrinol (Lausanne)
September 29, 2022
Muhammad Fauzi, Takaaki Murakami, Hiroyuki Fujimoto, Ainur Botagarova, Kentaro Sakaki, Sakura Kiyobayashi, Masahito Ogura, Nobuya Inagaki
examine the apoptosis rate, the slides were stained using
DeadEnd™ Fluorometric TUNEL System
(catalog no.
G3250
,
Promega
, Madison, WI), according to the manufacturer’s manual, as well as anti-insulin antibody and
(103)
Fibroblast growth factor receptor signaling in cardiomyocytes is protective in the acute phase following ischemia-reperfusion injury
Frontiers in Cardiovascular Medicine
September 23, 2022
Dzmitry Matsiukevich, Stacey L. House, Carla Weinheimer, Attila Kovacs, David M. Ornitz
Conjugate, Biotium,
1:100
dilution).
TUNEL
staining
was performed using the DeadEnd™ Fluorometric System (
G3250
,
Promega
) following the manufacturer's instructions. Quantitative
RT-PCR
was performed and analyzed
(104)
Pregabalin mitigates microglial activation and neuronal injury by inhibiting HMGB1 signaling pathway in radiation-induced brain injury
Journal of neuroinflammation
September 21, 2022
Zhang Z, Jiang J, He Y, Cai J, Xie J, Wu M, Xing M, Zhang Z, Chang H, Yu P,
et al
.
labeling
(
TUNEL) assay
Apoptotic cells were detected by
DeadEnd Fluorometric TUNEL System
(
Promega
,
G3250
) according to the manufacturer’s instructions, as previously described [4]. Briefly, the slices were
(105)
Effects of Elamipretide on Autophagy in Renal Cells of Pigs with Metabolic Syndrome
Cells
September 16, 2022
Siting Hong et al.
sections with terminal deoxynucleotide transferase-mediated
dUTP
nick-end
labeling
(
TUNEL
) (
Promega
, #
G3250
) and by calculating the ratio of renal mitochondrial to cytoplasmic cytochrome-c
protein expression
(106)
Mesenchymal Stem Cells and Formyl Peptide Receptor 2 Activity in Hyperoxia-Induced Lung Injury in Newborn Mice
International Journal of Molecular Sciences
September 13, 2022
Young Eun Kim, So Yoon Ahn, Dong Kyung Sung, Yun Sil Chang, Won Soon Park
staining
according to the manufacturer’s protocol using the
DeadEnd Fluorometric TUNEL System
kit (
G3250
;
Promega
, Madison, WI, USA). The
TUNEL
-stained lung area was counter-stained with 4′,6-diamidine-2′-phenylindole
(107)
MicroRNA-4516 in Urinary Exosomes as a Biomarker of Premature Ovarian Insufficiency
Cells
September 7, 2022
Zobia Umair, Mi-Ock Baek, Jisue Song, Seona An, Seung Joo Chon, Mee-Sup Yoon
Immunohistochemistry
Apoptosis in ovary tissues was detected by using a
DeadEnd™ Fluorometric TUNEL System
(
G3250
,
Promega
, Fitchburg, WI, USA), following the manufacturer’s instructions. The slides were examined by
(108)
Exercise Promotes Pro-Apoptotic Ceramide Signaling in a Mouse Melanoma Model
Cancers
September 2, 2022
Lee J, Savage H, Maegawa S, Ballarò R, Pareek S, Guerrouahen BS, Gopalakrishnan V, Schadler K.
prepared in accordance with the manufacturer’s protocol for the DeadEndTM fluorometric
TUNEL
system (
G3250
;
Promega
, Madison, WI, USA). Images (20× magnification) were processed using a Leica
fluorescence microscope
(109)
Pramlintide: A Novel Therapeutic Approach for Osteosarcoma through Metabolic Reprogramming
Cancers (Basel)
September 2, 2022
Yuanzheng Yang, Zhanglong Peng, Elsa R. Flores, Eugenie S. Kleinerman
(4.5.5). 2.7.
TUNEL
Staining
TUNEL
staining
was performed using the
DeadEnd Fluorometric TUNEL System
(
G3250
,
Promega
, Madison, WI, USA). Briefly, fresh
frozen sections
(5 µm) were air dried at room temperature
(110)
IRES-mediated Wnt2 translation in apoptotic neurons triggers astrocyte dedifferentiation
npj Regenerative Medicine
September 2, 2022
Hong Fan et al.
temperature in dark.
TUNEL
staining
was performed according to the manual of DeadENDTM
TUNEL
system (Cat:
G3250
,
Promega
). For combination of
TUNEL
staining
with
immunohistochemistry
,
TUNEL
staining
was performed
(111)
First Evidence of the Expression and Localization of Prothymosin α in Human Testis and Its Involvement in Testicular Cancers
Biomolecules
August 31, 2022
Venditti M, Arcaniolo D, De Sio M, Minucci S.
deoxynucleotidyl transferase
dUTP
nick end
labeling
(
TUNEL)-assay
using the
DeadEnd™ Fluorometric TUNEL System
(#
G3250
;
Promega
Corp., Madison, WI, USA) following the manufacturer’s protocol. The
cell nuclei
were marked
(112)
In vivo assessment of simultaneous G1 cyclins silencing by a tumor-specific bidirectional promoter on the mammary tumor in nude mice
Frontiers in Veterinary Science
August 22, 2022
Gholamreza Mesbah, Fatemeh Namazi, Fatemeh T. Shamsabadi, Zahra Maleki, Mehrab Nasirikenari, Majid Shahbazi
from
Santa Cruz Biotechnology
(Texas, United States). The DeadEndTM Fluorometric
TUNEL
System (Cat No.
G3250
) was purchased from
Promega
(Madison, USA). The bipSUR targets the G1 cyclins through a bidirectional
(113)
Atorvastatin combined with low-dose dexamethasone improves the neuroinflammation and survival in mice with intracerebral hemorrhage
Frontiers in Neuroscience
August 22, 2022
Yiming Song et al.
stained using a
TUNEL
(terminal deoxynucleotidyl transferase-
dUTP
nick end
labeling
)
staining
kit (
G3250
,
Promega
, Madison, WI, United States). Finally, the nuclei was stained by 4’,6-diamidino-2-phenylindole
(114)
Senolytic drugs relieve pain by reducing peripheral nociceptive signaling without modifying joint tissue damage in spontaneous osteoarthritis
Aging (Albany NY)
August 15, 2022
Tae-Hwan Gil, Haiyan Zheng, Hyo Gyeong Lee, Ji-Won Shin, Sun Wook Hwang, Ki-Mo Jang, Ok Hee Jeon
staining
as described above. The
DeadEnd™ Fluorometric TUNEL System
, tunnel
staining
kit (
Promega
, #
G3250
, USA) was used for
staining
per the manufacturer’s instruction. DAPI was added for counterstaining.
(115)
CircGSAP regulates the cell cycle of pulmonary microvascular endothelial cells via the miR-942-5p sponge in pulmonary hypertension
Frontiers in Cell and Developmental Biology
August 11, 2022
Yuanyuan Sun et al.
prepared based on the instructions in the
TUNEL
cell
apoptosis detection
kit (Dead End Fluorimetric Kit,
G3250
,
Promega
, United States). The samples were then observed under a
fluorescence microscope
, with an excitation
(116)
Efficacy of Nerve-Derived Hydrogels to Promote Axon Regeneration Is Influenced by the Method of Tissue Decellularization
International journal of molecular sciences
August 6, 2022
Kuna VK, Lundgren A, Anerillas LO, Kelk P, Brohlin M, Wiberg M, Kingham PJ, Novikova LN, Andersson G, Novikov LN.
temperature in the dark. The slides were coverslipped with ProLong mounting media containing DAPI (
G3250
,
Promega
Biotech AB, Nacka, Sweden). The
staining
specificity
was tested by omission of primary antibodies.
(117)
FGF1ΔHBS delays the progression of diabetic nephropathy in late-stage type 2 diabetes mouse model by alleviating renal inflammation, fibrosis, and apoptosis
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease
August 1, 2022
Qian Lin, Oscar Chen, John P. Wise, HongXue Shi, Kupper A. Wintergerst, Lu Cai, Yi Tan
apoptosis in kidney.
TUNEL assay
was performed using the
DeadEnd™ Fluorometric TUNEL System
(Cat No:
G3250
,
Promega Corporation
, US) according to the manufacturer’s instructions. In brief, sections were deparaffinized
(118)
Resveratrol, novel application by preconditioning to attenuate myocardial ischemia/reperfusion injury in mice through regulate AMPK pathway and autophagy level
Journal of Cellular and Molecular Medicine
August 1, 2022
Haiyan Li, Fuchun Zheng, Yanmei Zhang, Jiajia Sun, Fenfei Gao, Ganggang Shi
sectioned at a thickness of 4 μm and performed using the
DeadEnd™ Fluorometric TUNEL System
(
Promega
,
G3250
) following the manufacturer's protocols. DAPI solution (Beyotime) was used to mark the nucleus. A Zeiss
(119)
Maladaptive positive feedback production of ChREBPβ underlies glucotoxic β-cell failure
Nature communications
July 30, 2022
Katz LS, Brill G, Zhang P, Kumar A, Baumel-Alterzon S, Honig LB, Gómez-Banoy N, Karakose E, Tanase M, Doridot L,
et al
.
labeling
was performed according to the manufacturer, using the DeadEnd Fluormetric
TUNEL
System (Cat#
G3250
,
Promega
). Annexin V and PI
staining
were performed as described previously72 to distinguish dead (PI-AnnexinV+)
(120)
In vitro radiosensitization by eribulin in human cancer cell lines
Reports of Practical Oncology and Radiotherapy
July 29, 2022
Raquel Benlloch, Raquel Castejón, Silvia Rosado, María José Coronado, Patricia Sánchez, Jesús Romero
an indicator of apoptosis by
TUNEL assay
using the
DeadEnd™ Fluorometric TUNEL System
kit (
Promega
G3250
). Cells were cultured in 24-well plates. After 24 h, eribulin was added at 3 nM and 1.5 nM or 0.75 nM
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Reviews
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(1)
8/11/2015
Detecting and Quantifying Apoptotic Cells using DeadEnd Fluorometric TUNEL System
Lenin Veeraval
This kit was used to detect and quantify the apoptotic cells in a cell population treated with anticancer compounds compared to control cells. The product is simple to use and has high throughput.
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DeadEnd Fluorometric TUNEL System from Promega
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