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DeadEnd Fluorometric TUNEL System
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DeadEnd Fluorometric TUNEL System
DeadEnd Fluorometric TUNEL System
DeadEnd Fluorometric TUNEL System from Promega
(1)
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Citations:
(645)
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DeadEnd Fluorometric TUNEL System
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Description
DNA fragmentation detection assay. Designed for the detection and quantitation of apoptotic cells within a cell population
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
DeadEnd Fluorometric TUNEL System
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
G3250
Quantity
60 reactions
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Promega
2800 Woods Hollow Rd.
Madison
,
Wisconsin
United States
Phone
: 800-356-9526
Fax: 800-356-1970
Company Profile
Website:
http://www.promega.com
Citations (645)
(21)
NMOSD IgG Impact Retinal Cells in Murine Retinal Explants
Current Issues in Molecular Biology
September 7, 2023
Hannah Nora Wolf, Veronika Ehinger, Larissa Guempelein, Pratiti Banerjee, Tania Kuempfel, Joachim Havla, Diana Pauly
analyse apoptotic cell death,
TUNEL
staining
was performed using a DeadEnd Fluorometric
TUNEL
kit (#
G3250
,
Promega
, Fitchburg, WI, USA) following the manufacturer’s instructions. 2.4.
Quantitative Real-Time PCR
(22)
NRF2 Dysregulation in Mice Leads to Inadequate Beta-Cell Mass Expansion during Pregnancy and Gestational Diabetes
bioRxiv
September 2, 2023
Sharon Baumel-Alterzon, Isabelle Tse, Fatema Heidery, Adolfo Garcia-Ocaña, Donald K. Scott
performed according to the manufacturer instructions, using the
DeadEnd Fluorometric TUNEL System
(
G3250
;
Promega
). Samples were then immunostained with insulin antibody (
GTX39371
; Genetex). The percentage
(23)
L‐Arginine‐Loaded Gold Nanocages Ameliorate Myocardial Ischemia/Reperfusion Injury by Promoting Nitric Oxide Production and Maintaining Mitochondrial Function
Advanced Science
September 1, 2023
Zekun Wang, Nana Yang, Yajun Hou, Yuqing Li, Chenyang Yin, Endong Yang, Huanhuan Cao, Gaofei Hu, Jing Xue, Jialei Yang,
et al.
Apoptosis in the myocardial tissue region was analyzed using the DeadEnd™ Fluorescent
TUNEL
System (
G3250
,
Promega
).
Immunohistochemistry
and
immunofluorescence
were conducted to examine the expression of active
(24)
Metabolic transcriptomics dictate responses of cone photoreceptors to retinitis pigmentosa
Cell Reports
September 1, 2023
Sang Joon Lee, Douglas Emery, Eric Vukmanic, Yekai Wang, Xiaoqin Lu, Wei Wang, Enzo Fortuny, Robert James, Henry J. Kaplan, Yongqing Liu,
et al.
staining
, we used a commercially available fluorescent
TUNEL
kit (
DeadEnd Fluorometric TUNEL System
,
G3250
;
Promega
, USA) according to the manufacturer’s instructions. The
cell nuclei
were counterstained with
(25)
eIF3i promotes colorectal cancer cell survival via augmenting PHGDH translation
Journal of Biological Chemistry
September 1, 2023
Yaguang Zhang, Xiaowen Wan, Xuyang Yang, Xueqin Liu, Qing Huang, Lian Zhou, Su Zhang, Sicheng Liu, Qunli Xiong, Mingtian Wei,
et al.
terminal deoxynucleotidyl transferase–mediated dutp nick end
labeling
(
TUNEL
) System Kit (
Promega
,
G3250
) was used for the
TUNEL assay
according to the instruction. Briefly, the tissue sections were deparaffinized
(26)
Helicase-like transcription factor (Hltf)-deletion activates Hmgb1-Rage axis and granzyme A-mediated killing of pancreatic β cells resulting in neonatal lethality
PLoS One
August 25, 2023
Gurvinder Kaur, Rebecca A. Helmer, Dalia Martinez-Marin, Souad R. Sennoune, Rachel L. Washburn, Raul Martinez-Zaguilan, Jannette M. Dufour, Beverly S. Chilton
antifade reagent with DAPI (
P36935
). The DeadEndTM Fluorometric
TUNEL assay
(
Apoptosis Detection
System,
G3250
) was from
Promega Corporation
(Madison, WI). 10X Genomics (Pleasanton, CA) was the source of all Visium
(27)
Decreased Klotho Expression Causes Accelerated Decline of Male Fecundity through Oxidative Injury in Murine Testis
Antioxidants (Basel)
August 25, 2023
Ya-Yun Wang, Ying-Hung Lin, Vin-Cent Wu, Yu-Hua Lin, Chia-Yen Huang, Wei-Chi Ku, Chiao-Yin Sun
TUNEL Assay
Apoptotic cells in the testes were examined using the
DeadEnd Fluorometric TUNEL System
(
G3250
;
Promega
, Madison, WI, USA). The sections were deparaffinized, rehydrated, and fixed with 4% formaldehyde
(28)
Metabolic Alterations Caused by Simultaneous Loss of HK2 and PKM2 Leads to Photoreceptor Dysfunction and Degeneration
Cells
August 10, 2023
Eric Weh, Moloy Goswami, Sraboni Chaudhury, Roshini Fernando, Nick Miller, Heather Hager, Sarah Sheskey, Vikram Sharma, Thomas J. Wubben, Cagri G. Besirli
were rinsed and stained using the DeadEnd Fluorometric
TUNEL assay
(
Promega
, Madison, WI, USA, Cat#
G3250
) following the manufacturer’s instructions. As a negative control, retinal cell suspension from an attached
(29)
IMT504 protects beta cells against apoptosis and maintains beta cell identity, without modifying proliferation
Physiological reports
August 1, 2023
Converti A, Bianchi MS, Martinez MD, Montaner AD, Lux-Lantos V, Bonaventura MM.
with the IMT4 dose only.
TUNEL
labeling
was performed using the DeadEndFluorometric
TUNEL
System (#
G3250
,
Promega
) according to the manufacturer's instructions.
Confocal
images were obtained by an
IX83
Olympus
(30)
MAP4Ks inhibition promotes retinal neuron regeneration from Müller glia in adult mice
NPJ Regenerative medicine
July 13, 2023
Zhang H, Guo Y, Yang Y, Wang Y, Zhang Y, Zhuang J, Zhang Y, Shen M, Zhao J, Zhang R,
et al
.
methods.
TUNEL Assay
TUNEL assay
was performed using
DeadEnd Fluorometric TUNEL System
(
Promega
,
G3250
).
Frozen sections
of organs were rehydrated and incubated with
Proteinase K
in Tris/HCl (pH = 7.4,10
(31)
IGFBP1 Sustains Cell Survival during Spatially-Confined Migration and Promotes Tumor Metastasis
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
July 1, 2023
Cai G, Qi Y, Wei P, Gao H, Xu C, Zhao Y, Qu X, Yao F, Yang W.
cells in lungs were examined by
TUNEL
staining
using the
DeadEnd Fluorometric TUNEL System
(
Promega
,
G3250
) following the manufacturer's protocol.
Immunohistochemical
Analysis
Paraffin‐embedded tissue
sections
(32)
Transient neurogenesis in ischemic cortex from Sox2(+) astrocytes
Neural regeneration research
July 1, 2023
Yang JL, Fan H, Fu FF, Guo BL, Huang Y, Sun L, Wang WT, Xing JL, Hu XT, Ding YQ,
et al
.
were incubated for 3 hours at room temperature. To perform
TUNEL
staining
, DeadEND™
TUNEL
system (Cat#
G3250
,
Promega
, Madison, WI, USA) was used according to the product manual. For the combined
staining
of
TUNEL
(33)
Hypothalamic JNK1-hepatic fatty acid synthase axis mediates a metabolic rewiring that prevents hepatic steatosis in male mice treated with olanzapine via intraperitoneal: Additional effects of PTP1B inhibition
Redox Biology
July 1, 2023
Vitor Ferreira, Cintia Folgueira, María García-Altares, Maria Guillén, Mónica Ruíz-Rosario, Giada DiNunzio, Irma Garcia-Martinez, Rosa Alen, Christoph Bookmeyer, John G. Jones,
et al.
detection of cell death induced by the treatment with OLA by using the
DeadEnd™ Fluorometric TUNEL System
(
G3250
,
Promega
, Madison, WI, USA) according to supplier's instructions. Briefly, tissue sections (5 μm) were
(34)
Bioprosthetic Valve Deterioration: Accumulation of Circulating Proteins and Macrophages in the Valve Interstitium
JACC: Basic to Translational Science
July 1, 2023
Tomohisa Sakaue, Tadaaki Koyama, Yoshitsugu Nakamura, Keitaro Okamoto, Takayuki Kawashima, Tadashi Umeno, Yasuhide Nakayama, Shinji Miyamoto, Fumiaki Shikata, Mika Hamaguchi,
et al.
visualize the apoptotic cells in the
goat
tissues, the
DeadEnd Fluorometric TUNEL System
(catalog #
G3250
,
Promega
) was used according to the manufacturer’s protocol. Deparaffinized samples were fixed with
(35)
Astrocyte-oligodendrocyte interaction regulates central nervous system regeneration
Nature communications
June 8, 2023
Molina-Gonzalez I, Holloway RK, Jiwaji Z, Dando O, Kent SA, Emelianova K, Lloyd AF, Forbes LH, Mahmood A, Skripuletz T,
et al
.
a
Tunel assay
was performed prior to
staining
according to the manufacturer’s instructions (
Promega
G3250
). Ex vivo organotypic
mouse
brain explants Following terminal anaesthesia of P0-P2 CD-1
mouse
pups
(36)
Characterization of chimeric antigen receptor modified T cells expressing scFv-IL-13Rα2 after radiolabeling with (89)Zirconium oxine for PET imaging
Journal of translational medicine
June 7, 2023
Leland P, Kumar D, Nimmagadda S, Bauer SR, Puri RK, Joshi BH.
performed the
assay
using
DeadEnd fluorometric TUNEL system
as per manufacturer’s instructions (Cat#
G3250
,
Promega Corporation
, Madison, WI). After a brief wash with 1X PBS, 4 fields in each well were counted
(37)
Casein Kinase 2 Alpha Inhibition Protects against Sepsis-Induced Acute Kidney Injury
International Journal of Molecular Sciences
June 6, 2023
Jeung-Hyun Koo, Hwang Chan Yu, Seonhwa Nam, Dong-Chan Kim, Jun Ho Lee
Zeiss, Oberkochen, Germany). 4.7.
TUNEL Assay
TUNEL
staining
was used to detect apoptotic cells (#
G3250
,
Promega
, Madison, WI, USA). In brief,
after treatment
with a nucleotide mix and rTdT (terminal deoxynucleotide
(38)
Myoglobin-derived iron causes wound enlargement and impaired regeneration in pressure injuries of muscle
eLife
June 2, 2023
Nasir NJM, Heemskerk H, Jenkins J, Hamadee NH, Bunte R, Tucker-Kellogg L.
saline-treated mPI and DFO-treated mPI (8 μm sections) were stained with the
DeadEnd Fluorometric TUNEL System
(
G3250
;
Promega
, WI) according to the manufacturer’s protocol. DAPI was used for nuclear
staining
. Images were
(39)
Srs11‐92, a ferrostatin‐1 analog, improves oxidative stress and neuroinflammation via Nrf2 signal following cerebral ischemia/reperfusion injury
CNS Neuroscience & Therapeutics
June 1, 2023
Yuhua Chen, Wei He, Hong Wei, Cuicui Chang, Lingjian Yang, Jiao Meng, Tianlin Long, Quanhua Xu, Chi Zhang
the sections were dehydrated and mounted. For
TUNEL assay
, a
DeadEnd™ fluorometric TUNEL system
(Cat:
G3250
;
Promega
, Madison, WI, USA) was used according to the manufacturer's directions, followed by
incubation
(40)
Conserved transcriptional connectivity of regulatory T cells in the tumor microenvironment informs new combination cancer therapy strategies
Nature Immunology
June 1, 2023
Ariella Glasner, Samuel A. Rose, Roshan Sharma, Herman Gudjonson, Tinyi Chu, Jesse A. Green, Sham Rampersaud, Izabella K. Valdez, Emma S. Andretta, Bahawar S. Dhillon,
et al.
were processed under standardized conditions using the DeadEnd Fluorometric Detection System (
Promega
,
G3250
), and subsequent
immunohistochemistry
was carried out using BOND Polymer Refine Detection Kit (Leica,
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Reviews
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(1)
8/11/2015
Detecting and Quantifying Apoptotic Cells using DeadEnd Fluorometric TUNEL System
Lenin Veeraval
This kit was used to detect and quantify the apoptotic cells in a cell population treated with anticancer compounds compared to control cells. The product is simple to use and has high throughput.
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DeadEnd Fluorometric TUNEL System from Promega
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