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Assay Kit
DeadEnd Fluorometric TUNEL System
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DeadEnd Fluorometric TUNEL System
DeadEnd Fluorometric TUNEL System
DeadEnd Fluorometric TUNEL System from Promega
(1)
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Citations:
(645)
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DeadEnd Fluorometric TUNEL System
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Description
DNA fragmentation detection assay. Designed for the detection and quantitation of apoptotic cells within a cell population
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
DeadEnd Fluorometric TUNEL System
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
G3250
Quantity
60 reactions
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Promega
2800 Woods Hollow Rd.
Madison
,
Wisconsin
United States
Phone
: 800-356-9526
Fax: 800-356-1970
Company Profile
Website:
http://www.promega.com
Citations (645)
(81)
Visceral adipose tissue remodeling in pancreatic ductal adenocarcinoma cachexia: the role of activin A signaling
Scientific Reports
December 1, 2022
Pauline C. Xu et al.
FL). TdT-mediated
dUTP
nick-end
labeling
(
TUNEL
)
TUNEL
was performed using the
DeadEnd™ Fluorometric TUNEL System
(
G3250
,
Promega
, Madison, WI). Light
and fluorescence microscopy
All images were taken with the
EVOS M7000 Imaging System
(82)
Synthetic lethality between TP53 and ENDOD1
Nature Communications
December 1, 2022
Zizhi Tang et al.
assays
were performed following the instructions of
DeadEnd™ Fluorometric TUNEL System
kit (
Promega
,
G3250
). Briefly, cells were fixed in freshly prepared 4% methanol-free formaldehyde PBS solution for 25 min
(83)
Elevated testicular apoptosis is associated with elevated sphingosine driven by gut microbiota in prediabetic sheep
BMC Biology
December 1, 2022
Yuanchao Sun, Peng Sun, Yanting Hu, Liying Shan, Qi Geng, Yutian Gong, Haitao Fan, Teng Zhang, Yang Zhou
gene was rectified by expression of Gapdh.
TUNEL assay
A
DeadEnd Fluorometric TUNEL System
(
Promega
,
G3250
) was used in
TUNEL assay
. According to the instructions, tissue sections were stained with sufficient
(84)
Altered proteome in translation initiation fidelity defective eIF5G31R mutant causes oxidative stress and DNA damage
Scientific Reports
December 1, 2022
Anup Kumar Ram, Monalisha Mallik, R. Rajendra Reddy, Amol Ratnakar Suryawanshi, Pankaj V. Alone
(1.52p).
Tunel assay
Tunel assay
was performed using
DeadEnd Fluorometric Tunel System
(
Promega
#
G3250
).
Yeast
cells OD600 ~ 0.8 (100 μl) was spun down at 4000×g for 2 min, followed by fixation in 3.7% formaldehyde
(85)
Adipose stem cell-derived extracellular vesicles ameliorates corticosterone-induced apoptosis in the cortical neurons via inhibition of ER stress
Stem Cell Research & Therapy
December 1, 2022
Sung-Ae Hyun et al.
performed according to manufacturer’s instructions (
DeadEnd™ Fluorometric TUNEL System
, catalog number:
G3250
,
Promega
, Madison, WI, USA) to detect cell death in the cultured cortical
neuron
. The
assay
stained
(86)
Tolerogenic nanoparticles induce type II collagen-specific regulatory T cells and ameliorate osteoarthritis
Science advances
November 25, 2022
Sohn HS, Choi JW, Jhun J, Kwon SP, Jung M, Yong S, Na HS, Kim JH, Cho ML, Kim BS.
kit (
DeadEnd fluorometric TUNEL system
) according to the manufacturer’s instruction (
Promega
, USA,
G3250
).
PCR
of the whole joint mRNA levels of inflammatory and anti-inflammatory cytokines in the joints
(87)
Mutation of Leaf Senescence 1 Encoding a C2H2 Zinc Finger Protein Induces ROS Accumulation and Accelerates Leaf Senescence in Rice
International Journal of Molecular Sciences
November 21, 2022
Chao Zhang, Ni Li, Zhongxiao Hu, Hai Liu, Yuanyi Hu, Yanning Tan, Qiannan Sun, Xiqin Liu, Langtao Xiao, Weiping Wang,
et al.
previously described [77]. Apoptosis was detected using a
DeadEnd Fluorometric TUNEL system
(
Promega
, #
G3250
, USA) according to the manufacturer’s instructions. 4.8. Subcellular Localization To further determine
(88)
METTL14 is required for exercise-induced cardiac hypertrophy and protects against myocardial ischemia-reperfusion injury
Nature communications
November 9, 2022
Wang L, Wang J, Yu P, Feng J, Xu GE, Zhao X, Wang T, Lehmann HI, Li G, Sluijter JPG,
et al
.
was used to quantify cell size. For
TUNEL
staining
, Dead End Fluorometric
TUNEL
System (
Promega
, Cat#
G3250
) were used to perform
TUNEL
staining
on 5 μm frozen heart sections according to the manufacturer’s protocol
(89)
Neurovascular injury associated non-apoptotic endothelial caspase-9 and astroglial caspase-9 mediate inflammation and contrast sensitivity decline
Cell Death Dis
November 8, 2022
Crystal Colón Ortiz, Albertine M. Neal, Maria I. Avrutsky, Monica Choi, Jade Smart, Jacqueline Lawson, Carol M. Troy
0.1% Triton X-100 for two hours.
TUNEL
was then done following the manufacturer’s protocol (
Promega
G3250
). Sections were then processed
for IHC
as described above. Analysis of
cell counts
Images were first
(90)
BAF60c prevents abdominal aortic aneurysm formation through epigenetic control of vascular smooth muscle cell homeostasis
Journal of Clinical Investigation
November 1, 2022
Guizhen Zhao, Yang Zhao, Haocheng Lu, Ziyi Chang, Hongyu Liu, Huilun Wang, Wenying Liang, Yuhao Liu, Tianqing Zhu, Oren Rom,
et al.
section) for each
mouse
was calculated.
Apoptosis assay
. The
DeadEnd Fluorometric TUNEL system
(
Promega
,
G3250
) was used to detect cell apoptosis according to the manufacturer’s protocol. Briefly, to detect cell
(91)
Optic neuropathy associated with TGFβ dysregulation in mice with a glaucoma-causative mutation of ADAMTS10
Matrix Biology
November 1, 2022
Hang-Jing Wu, Rachel W. Kuchtey, John Kuchtey
were detected by terminal deoxynucleotidyl transferase mediated
dUTP
nick-end labelling (
TUNEL
, Cat. #
G3250
,
Promega
, USA) following manufacturer’s instructions. Images were acquired using an upright
fluorescence microscope
(92)
MEK1/2 inhibition rescues neurodegeneration by TFEB-mediated activation of autophagic lysosomal function in a model of Alzheimer’s Disease
Molecular Psychiatry
November 1, 2022
Yoon Sun Chun, Mi-Yeon Kim, Sun-Young Lee, Mi Jeong Kim, Tae-Joon Hong, Jae Kyong Jeon, Dulguun Ganbat, Hyoung Tae Kim, Sang Seong Kim, Tae-In Kam,
et al.
dehydrogenase (LDH)
assay
DNA fragmentation was determined using
DeadEnd™ Fluorometric TUNEL System
(
Promega
,
G3250
) according to the supplier’s instruction. Briefly, cells were fixed, permeabilized, labeled with the
(93)
Exercise attenuates angiotensinⅡ-induced muscle atrophy by targeting PPARγ/miR-29b
Journal of sport and health science
November 1, 2022
Liu Q, Chen L, Liang X, Cao Y, Zhu X, Wang S, Li J, Gao J, Xiao J.
KGY00850; KeyGEN) at room temperature for 2 h, incubated with the
DeadEnd Fluorometric TUNEL System
(
G3250
;
Promega
) at 37°C for 1 h, and stopped with 2 × saline-sodium citrate (SSC, G329; Promega). The
frozen sections
(94)
Circular RNA circBNC2 inhibits epithelial cell G2-M arrest to prevent fibrotic maladaptive repair
Nature communications
October 31, 2022
Wang P, Huang Z, Peng Y, Li H, Lin T, Zhao Y, Hu Z, Zhou Z, Zhou W, Liu Y,
et al
.
staining
, a
DeadEnd Fluorometric TUNEL System
was applied according to manufacturer’s instructions (
G3250
,
Promega
). The degree of
hepatocyte
apoptosis for each sample was quantified by an experienced pathologist
(95)
OsMADS58 Stabilizes Gene Regulatory Circuits during Rice Stamen Development
Plants (Basel)
October 28, 2022
Liping Shen, Feng Tian, Zhukuan Cheng, Qiang Zhao, Qi Feng, Yan Zhao, Bin Han, Yuhan Fang, Yanan Lin, Rui Chen,
et al.
TUNEL assay
was performed using the
DeadEnd Fluorometric TUNEL system
(
Promega
, Madison, MI, USA, #
G3250
), according to the manufacturer’s instructions. Signals were observed and imaged using an Imager D2
(96)
Intrapericardial Exosome Therapy Dampens Cardiac Injury via Activating Foxo3
Circulation Research
October 28, 2022
Dashuai Zhu, Shuo Liu, Ke Huang, Zhenzhen Wang, Shiqi Hu, Junlang Li, Zhenhua Li, Ke Cheng
was used for nucleus
staining
.
TUNEL
staining
was performed by referring to the user manual (
Promega
,
G3250
). The antibodies used in this study were listed in Supplementary Table 2. Imaging was performed using
(97)
Downregulation of hepatic ceruloplasmin ameliorates NAFLD via SCO1-AMPK-LKB1 complex
Cell reports
October 18, 2022
Xie L, Yuan Y, Xu S, Lu S, Gu J, Wang Y, Wang Y, Zhang X, Chen S, Li J,
et al
.
staining
assay
was performed using the
DeadEnd™ Fluorometric TUNEL System
(
Promega Corporation
, USA;
G3250
) following the manufacturer’s instructions. For Ki67,
Ndufb8
and
Hmgcs2
staining
, the sections were
(98)
Hypoxia-Inducible Factor-1α in Rods Is Neuroprotective Following Retinal Detachment
Investigative ophthalmology & visual science
October 3, 2022
Ross BX, Jia L, Kong D, Wang T, Yao J, Hager HM, Abcouwer SF, Zacks DN.
optic nerve head. To evaluate cell death, the
TUNEL assay
(
DeadEnd Fluorometric TUNEL System
catalog #
G3250
;
Promega
, Madison, WI, USA) was performed at 3 days post RD according to the manufacturer's instructions.
(99)
Exocyst inactivation in urothelial cells disrupts autophagy and activates non-canonical NF-κB signaling
Disease Models & Mechanisms
October 1, 2022
Michael A. Ortega, Ross K. Villiger, Malia Harrison-Chau, Suzanna Lieu, Kadee-Kalia Tamashiro, Amanda J. Lee, Brent A. Fujimoto, Geetika Y. Patwardhan, Joshua Kepler, Ben Fogelgren
apoptotic cells in formalin-fixed histological sections,
DeadEnd Fluorometric TUNEL System
(
Promega
,
G3250
) was used according to the manufacturer's instructions. The primary antibodies used were as follows:
(100)
Melatonin drives apoptosis in head and neck cancer by increasing mitochondrial ROS generated via reverse electron transport
Journal of Pineal Research
October 1, 2022
Javier Florido, Laura Martinezâ€Ruiz, César Rodriguezâ€Santana, Alba Lópezâ€RodrÃguez, AgustÃn Hidalgoâ€Gutiérrez, Cécile Cottetâ€Rousselle, Frédéric Lamarche, Uwe Schlattner, Ana Guerraâ€Librero, Paula Arandaâ€MartÃnez,
et al.
using the DeadEnd Fluorometric terminal deoxynucleotidyl transferase
dUTP
nick end
labeling
(
TUNEL
) (
G3250
;
Promega
) system in
tumor
tissue sections following the manufacturer's indications. Hoechst (33342;
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Reviews
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(1)
8/11/2015
Detecting and Quantifying Apoptotic Cells using DeadEnd Fluorometric TUNEL System
Lenin Veeraval
This kit was used to detect and quantify the apoptotic cells in a cell population treated with anticancer compounds compared to control cells. The product is simple to use and has high throughput.
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DeadEnd Fluorometric TUNEL System from Promega
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