DeadEnd Fluorometric TUNEL System G3250 from Promega

Product Specs
ItemDeadEnd Fluorometric TUNEL System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberG3250
Quantity60 reactions

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Protective Effect of Brain Derived Neurotrophic Factor-Overexpressing Wharton's Jelly-Derived Mesenchymal Stromal Cells in Severe Intraventricular Hemorrhage in Newborn RatsInternational Journal of Stem CellsFebruary 28, 2028So Yeon Jung, Misun Yang, Young Eun Kim, Dong Kyung Sung, Se In Sung, Chang-Woo Lee, Yun Sil Chang, So Yoon Ahnfluorescence following the manufacturer’s instructions for the DeadEnd Fluorometric TUNEL System kit (G3250; Promega) and counterstained with DAPI. TUNEL-positive cells were observed under a confocal laser scanning

(2) KLS-3021: Innovative Oncolytic Virotherapy for the Treatment of Advanced Primary and Metastatic Cutaneous Squamous Cell CarcinomaMolecular Therapy OncologyJune 18, 2026Kiwon Park, Jinwon Seo, Sungmin Lee, Hyesoo Kang, Joonsung Kim, Eunjin Lee, Jaeil Shin, Soon-Oh Hong, Sujeong Kim, Sun Jin Kim1:100). The TUNEL assay was performed with the DeadEnd Fluorometric TUNEL System (Promega, cat. no. G3250) according to the manufacturer’s instructions. Following phosphate-buffered saline washes, sections

(3) GPR124 regulates hyaloid blood vessel regression and is associated with endothelial-mesenchymal transitionSci RepApril 29, 2026Laura Hannig, Robin Heiden, Süleyman Ergün, Barbara M. Braunger, Mario Valloneach), followed by labeling apoptotic cells using the Dead-End Fluorometric TUNEL System (Promega #G3250) according to manufacturer’s instructions. Flatmounts were washed three times with PBS (5 min each)

(4) Targeting of Itch by Clomipramine or Gene Therapy improves cognitive defects related to Alzheimer's DiseaseiScienceApril 17, 2026Monika Chauhan, Komal Singh, Pushkar Sharmaassays,Critical commercial assays,Critical commercial assays ,, DeadEnd TUNEL Fluorometric kit,Promega,Cat#G3250 ,, Experimental models: Cell lines,Experimental models: Cell lines,Experimental models: Cell lines ,, HEK293,ATCC,Cat#

(5) Redox imbalance dictates dependence on GOT1 versus GOT2 for rod photoreceptor health during aging and stressbioRxivApril 7, 2026Meini Chen, Eric Weh, Moloy T. Goswami, Katherine M. Weh, Heather Hager, Peter Sajjakulnukit, Avi Weingarten, Shubha Subramanya, Nicholas Miller, Sraboni Chaudhury, et al.were washed and resuspended before staining using the DeadEnd Fluorometric TUNEL kit (Promega, Cat #G3250). Stained samples were analyzed using an Attune NXT flow cytometer (Thermo Fisher Scientific; FITC channel

(6) Pterostilbene attenuates lung ischemia-reperfusion injury: integrative insights from network pharmacology, molecular dynamics, and experimental validationFront PharmacolApril 1, 2026Haotian Bai, Heng Zhao, Jinteng Feng, Hongyi Wang, Yixing Li, Zhe Chen, Bin He, Chi Wang, Rui Gao, Rui Zhao, et al.gradient, embedded in paraffin, and sectioned at 4 µm. Sections were stained using a TUNEL assay kit (G3250, Promega, United States) following the manufacturer’s instructions and counterstained with DAPI. Apoptotic

(7) Transcription factor NFYA directs male meiotic entry by regulating accessible chromatin at meiotic promoters in miceThe EMBO JournalMarch 19, 2026Martin Säflund, Masomeh Askari, Atiyeh Eghbali, Mukhtar Mohamed Abdi, Dilay Deren Er, Ann-Kristin Östlund Farrants, Tianxiong Yu, Deniz M ÖzataNick-End Labeling (TUNEL) assay was performed using the DeadEnd Fluorometric TUNEL System (Promega; G3250) according to the manufacturer’s protocol. In all, 5-μm tissue sections were first deparaffinized in

(8) IncRNAs transcriptomics elucidates the potential mechanism of Naoshuantong capsule in alleviating synaptic dysfunction in a murine model of cerebral ischemia/reperfusion injuryFront PharmacolMarch 5, 2026Ke Song, Hongrui Zhang, Haoqi Liu, Yuanyuan Li, Yikun Sun, Xinglu Dong, Chenxi Tao, Yannan He, Zhenhong Liu, Yonghong Gao, et al.DeadEnd™ Fluorometric TUNEL System was sourced from Promega Corporation (Madison, Wisconsin, United States; Catalog Number: G3250). RIPA lysis buffer was procured from Beijing Pulilai Gene Technology Co., Ltd. (Beijing, China; Catalog

(9) NanoScript-Enabled Nonviral Transient Repression of Phosphatase and Tensin Homolog for Axonal Regeneration and Central Nervous System Injury RepairACS NanoMarch 3, 2026Brandon Conklin, Yanting Liu, Sarah Nevins, Byeong-Gwan Song, Sy-Tsong Dean Chueng, Qiu Xiaowen, Sungyun Kim, Heyin Cheung, Seong Bae An, JongMin Lee, et al.cells in the spinal cord tissue were detected using the DeadEnd Fluorometric TUNEL System (Cat. No. G3250; Promega, Madison, WI, USA) following the manufacturer’s protocol. Paraffin-embedded sections were deparaffinized

(10) H3K9me2 is a determinant for the mitosis-to-meiosis transition in female germ cellsCell Death DisMarch 2, 2026Yanting Hu, Heyang Zhou, Lining Shi, Ke Liu, Xiangyue Meng, Xinru Guo, Liying Shan, Futeng Hu, Yongbin Liu, Teng Zhang, et al.fluorescence microscope. TUNEL assay TUNEL assay was performed using a DeadEndTM Fluorescence TUNEL System (Promega, G3250). According to instructions, store in a humidified environment at 37 °C for 60 min with sufficient rTdT

(11) Botulinum toxin-induced masseter muscle atrophy is associated with impaired autophagic flux without signs of apoptosis in miceCell Death DiscoveryFebruary 28, 2026Esteban R. Quezada, Noelia Blanco, Paola Llanos, Alfredo Criollo, Mario Chiong, Sonja BuvinicObject Research Systems, Canada). TUNEL The TUNEL Kit (DeadEnd™ Fluorometric TUNEL System, Promega, G3250, WI, USA) was used on the samples mounted on the slide. 100 μL of equilibration buffer was added for

(12) LRP1 in Adult-Born Neural Stem Cells Modulates Neurogenesis and Hippocampal MemoryCellsFebruary 28, 2026Kristi Dietert, Nicole Marion, Meng Wang, Pamela Reed, Erzsebet Kokovay, Naomi L. Sayresubjected to the TUNEL procedure, which was performed using DeadEnd™ Fluorometric TUNEL System (Promega G3250, Madison, WI, USA) according to the manufacturer’s protocol. Immediately following the TUNEL procedure,

(13) A de novo H3.2K9me2 deposition pathway establishes heterochromatin for suppressing transposon mobilization during fly somatic developmentNucleic Acids ResearchFebruary 27, 2026Yi Ni Luo, Yazi Deng, Yu Liang, Wei Wu, Wei Wu, Lu Wanglabeling) experiments were performed by using the DeadEnd Fluorometric TUNEL System (Promega, CAT#: G3250) following the standard protocol in this kit. Briefly, 5–7 pupal hindguts were dissected in cold PBS.

(14) Mitochondrial permeability transition pore desensitization by a novel dispiranic derivative prevents cardiac reperfusion injuryRedox BiologyFebruary 19, 2026Giampaolo Morciano, Gaia Pedriali, Giulia Turrin, Cristina Algieri, Esther Densu Agyapong, Debora La Mantia, Chiara Bernardini, Giorgia Canini, Anna Fantinati, Elena Nicoletta Colarusso, et al.transferase-mediated deoxyuridine 5-triphosphate nick-end labeling (TUNEL) assay (DeadEnd™ Fluorometric TUNEL System, G3250, Promega) to identify fragmented nuclei in the tissue. Before immunochemistry staining the samples were

(15) KLS-3021, a multifunctional oncolytic virus, demonstrates potent early-intervention efficacy in preclinical models of prostate-confined and locally advanced prostate cancerFrontiers in OncologyFebruary 13, 2026Jinwon Seo, Kiwon Park, Sungmin Lee, Hyesoo Kang, Joonsung Kim, Eunjin Lee, Jaeil Shin, Soon-Oh Hong, Sujeong Kim, Sun Jin Kimrespectively. The TUNEL assay was performed using the DeadEnd Fluorometric TUNEL System (Promega, Cat. No. G3250) according to the manufacturer’s instructions, followed by co-immunostaining with CD31 (BD Pharmingen)

(16) Mild hypothermia (35°C) reduces myocardial ischemia-reperfusion injury and attenuates hypoxia induced apoptosis of H9C2 cardiomyocytes by changing the phosphorylation level of Connexin43 (Cx43) proteinAfrican Health SciencesFebruary 5, 2026Yuanming Lu, Fang Wang, Nanping Xiao, Zilian Fan, Lan Xiong, Jing Kou, Tianxun Wang, Dianyuan Liincubate at room temperature for 15 minutes, and PBS was washed for 2-3 times. Then TUNEL test kit (G3250, Promega, Madison, WI, USA) was used to detect DNA fragments broken during apoptosis. Dye the nucleus

(17) Gestational progesterone restores menstrual cycle in PCOS patients via enhancing ovary estrogen productionLife MetabolismFebruary 2, 2026Qiwen Yang, Na Kong, Jing Wu, Yan Yang, Xinge Zhang, Yuan Lin, Zhibin Hu, Guijun Yan, Haixiang Sun, Chaojun Liin 10 mmol/L citrate buffer, pH 6.0) and rinsed in PBS. The cell death detection kit TUNEL (Promega, G3250) was used, and the protocol of the TUNEL manufacturer was followed. All sections were examined under

(18) Mechanistic Insights Into NFIX‐Mediated DNA Recognition and Transcriptional Regulation in Skeletal MuscleSmart MedicineFebruary 1, 2026Ci Zhu, Shuang Liu, Xi Chen, Chengxiao Qin, Yueyu Wang, Chunchun Xue, Lingxing Li, Wenlan Du, Xin Chen, Xiaofeng Li, et al.replicates. 4.7 TUNEL Assay Apoptotic cells were detected using the DeadEnd Fluorometric TUNEL System (Promega, #G3250) according to the manufacturer's instructions. Human skeletal muscle cells were seeded in a 35‐mm glass‐bottom

(19) Oxidized MIF is an Alzheimer’s disease drug target relaying external risk factors to tau pathologyCell Reports MedicineJanuary 20, 2026Andreas Müller-Schiffmann, Felix Torres, Anatoliy Kitaygorodskyy, Anand Ramani, Argyro Alatza, Sarah K. Tschirner, Julien Orts, Arthur Haltrich, Ingrid Prikulis, Shaofeng Yu, et al.DC Protein Assay Kit I,BioRad,5000111 IsoStrip,Roche,11493027001 PepSpots with 52 MIF peptides,JPT,N/A DeadEnd Fluorometric TUNEL System,Promega,G3250 QuikChange Site-directed Mutagenesis Kit,Stratagene,200519 ,, Deposited data,Deposited data,Deposited

(20) p16INK4a promotes myocardial ischemia-reperfusion injury by regulating bile acid transport via Slco1a4EMBO ReportsJanuary 12, 2026Tingting Yang, Qiulian Zhou, Yihua Bei, Danni Meng, Songwei Ai, Yuhui Zhang, Jian Zhang, Li Liu, Hongjian Chen, Xue Pan, et al.A111-03), while apoptosis in heart tissue was assessed using Dead End Fluorometric TUNEL System (Promega, G3250) according to the manufacturer’s protocol. The cell nuclei were stained with Hoechst (1:2000, KeyGEN,

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8/11/2015Detecting and Quantifying Apoptotic Cells using DeadEnd™ Fluorometric TUNEL System Lenin VeeravalThis kit was used to detect and quantify the apoptotic cells in a cell population treated with anticancer compounds compared to control cells. The product is simple to use and has high throughput.

DeadEnd Fluorometric TUNEL System

DeadEnd Fluorometric TUNEL System from Promega

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DeadEnd Fluorometric TUNEL System from Promega

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