DeadEnd Fluorometric TUNEL System G3250 from Promega

Product Specs
ItemDeadEnd Fluorometric TUNEL System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberG3250
Quantity60 reactions

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Protective Effect of Brain Derived Neurotrophic Factor-Overexpressing Wharton's Jelly-Derived Mesenchymal Stromal Cells in Severe Intraventricular Hemorrhage in Newborn RatsInternational Journal of Stem CellsFebruary 28, 2028So Yeon Jung, Misun Yang, Young Eun Kim, Dong Kyung Sung, Se In Sung, Chang-Woo Lee, Yun Sil Chang, So Yoon Ahnfluorescence following the manufacturer’s instructions for the DeadEnd Fluorometric TUNEL System kit (G3250; Promega) and counterstained with DAPI. TUNEL-positive cells were observed under a confocal laser scanning

(2) Black Phosphorus Nanosheets Penetrate the Blood–Testis Barrier and Induce Reproductive Toxicity in Male MiceEnviron Health (Wash)June 19, 2026Suhua Jiang, Fengkai Ruan, Fucong Zhang, Zewei Long, Xiaoyan Ding, Sihao Zhu, Zhineng Fu, Zhenghong Zuo, Xiaoming Jiang, Chengyong Hethe examination of apoptosis. The TUNEL assay was conducted with a DeadEnd Fluorometric TUNEL System (G3250, Promega, China) in accordance with the specifications outlined by the manufacturer. Apoptotic cells

(3) KLS-3021: Innovative Oncolytic Virotherapy for the Treatment of Advanced Primary and Metastatic Cutaneous Squamous Cell CarcinomaMolecular Therapy OncologyJune 18, 2026Kiwon Park, Jinwon Seo, Sungmin Lee, Hyesoo Kang, Joonsung Kim, Eunjin Lee, Jaeil Shin, Soon-Oh Hong, Sujeong Kim, Sun Jin Kim1:100). The TUNEL assay was performed with the DeadEnd Fluorometric TUNEL System (Promega, cat. no. G3250) according to the manufacturer’s instructions. Following phosphate-buffered saline washes, sections

(4) Oncogenic KRAS-driven type I interferon signalling primes pancreatic cancer for necroptosisNat CommunJune 15, 2026Sofya Tishina, Alina Dahlhaus, Marta Manik, Lejla Mulalic, Janine Murr, Michael Kotliar, Hassan Rakhsh-Khorshid, Myrto Kostopoulou, Florian Hocher, Jenny Stroh, et al.to the study design. TUNEL stainings were performed according to manufacturer’s protocol (Promega, G3250) for paraffin embedded tissue. The propidium iodide step was omitted, and slides were instead covered

(5) 17-DMAG-Loaded HER2-Targeted Extracellular Vesicles Induce PARP/Caspase3-Mediated Apoptosis in Gastric CarcinomaInt J Mol SciJune 15, 2026Sin Hye Park, Deok Yong Sim, Do Sang Lee, Chan Mi Lee, Joo Won Moon, Ji Won Choi, Dong Jin Kimassessed using the DeadEndTM Fluorometric TUNEL system kit according to Promega’s instructions (Cat No G3250, Promega, Madison, WI, USA). In brief, NCI-N87 cells treated with 17-DMAG, Ct-Exo17-DMAG, and p51-Exo17-DMAG

(6) Monoclonal antibodies targeting PCDH7 inhibit tumor growth and enhance immune responses in KRAS-mutant non-small cell lung cancerSci AdvJune 5, 2026Nicole Novaresi, Poorva Ghosh, Shayna Thomas-Jardin, Hui Deng, Xuejun Fan, Zhiqiang Ku, Wei Xiong, Xiaorong Zhou, Jingfei Zhu, Huiyu Li, et al.formalin-fixed paraffin-embedded tumor tissue sections were performed using the DeadEnd Fluorometric TUNEL System (Promega, G3250) according to the manufacturer’s instructions. Slides were mounted using Vectashield Hardset antifade

(7) Chemical priming potentiates mesothelin-targeting chimeric antigen receptor-engineered NK-92 antitumor activity by improving tumor trafficking and cytotoxic killing dynamicsFront ImmunolJune 1, 2026Ki Seo Ryu, Hail Park, Eunchong Maeng, Jun-Yeon Lim, Duck Cho, Seung Hee Choi, Kyung-Soon ParkTUNEL assay Apoptotic cell death in tumor tissues was assessed using the DeadEnd™ Fluorometric TUNEL System (G3250, Promega) according to the manufacturer’s instructions. Briefly, tumor tissues were embedded in OCT

(8) Fibroblast Growth Factor Receptor Signaling in Maturing Osteoblasts Controls Cell-Matrix Interactions Critical for Osteocyte SurvivalbioRxivMay 5, 2026Debabrata Patra, Craig Smith, Chenming Wei, Courtney M. Mazur, Ilemona Ameadaji, Tiandao Li, Marc N. Wein, Mathew J. Silva, David M. Ornitzwere performed on FFPE sections using the DeadEnd™ Fluorometric TUNEL System staining kit (Promega, G3250) and manufacturer’s instructions provided. Images of stained bone sections were captured at high resolution

(9) Cuproptosis and Disulfidptosis Converge to Empower PD‐L1 Checkpoint Therapy via Cadict‐Induced PD‐L1 TranslationAdvanced ScienceMay 1, 2026Shaoqing Huang, Shiyao Song, Xinhua Zhang, Jialing Gao, Ruihan Pu, Jiatong Dai, Xiaoxue Wu, Lulu Chen, Qinghai Li, Xiaoting Liu, et al.CRC tissue, a tissue apoptosis assay was performed using a Fluorometric TUNEL assay kit (Promega, #G3250). Immunofluorescent staining for tumor tissue was performed under the same conditions as the cells processing

(10) GPR124 regulates hyaloid blood vessel regression and is associated with endothelial-mesenchymal transitionSci RepApril 29, 2026Laura Hannig, Robin Heiden, Süleyman Ergün, Barbara M. Braunger, Mario Valloneach), followed by labeling apoptotic cells using the Dead-End Fluorometric TUNEL System (Promega #G3250) according to manufacturer’s instructions. Flatmounts were washed three times with PBS (5 min each)

(11) Targeting of Itch by Clomipramine or Gene Therapy improves cognitive defects related to Alzheimer's DiseaseiScienceApril 17, 2026Monika Chauhan, Komal Singh, Pushkar Sharmaassays,Critical commercial assays,Critical commercial assays ,, DeadEnd TUNEL Fluorometric kit,Promega,Cat#G3250 ,, Experimental models: Cell lines,Experimental models: Cell lines,Experimental models: Cell lines ,, HEK293,ATCC,Cat#

(12) Uttroside B, a US FDA-designated ‘Orphan Drug’, mitigates the development of hepatocellular carcinoma and its pulmonary metastasis via EGFR/ERK-mediated inhibition of SREBP-1 and STAT-3Cell Death DiscoveryApril 16, 2026Chenicheri Kizhakkeveettil Keerthana, Tennyson P. Rayginia, Kalishwaralal Kalimuthu, Sandhini Saha, Nair H. Haritha, Amrutha Nisthul Areekkara, Mundanattu Swetha, Sreekumar U. Aiswarya, Lekshmi R. Nath, Sanjay Suresh Varma, et al.was purchased from Invitrogen. DeadEnd™ Fluorimetric TUNEL System was procured from Promega (Cat. No. G3250). Silencer Select EGFR siRNA and negative control siRNA were purchased from Thermo Fisher Scientific.

(13) Interleukin 27-induced photoreceptor survival is associated with suppression of a novel Muller glia subpopulationCell Communication and SignalingApril 16, 2026Laura D. McGee, Brandon M. Bessen, James J. Dollar, Marco Galizia, Saili Khorjekar, Ashley N. Zuniga, Stefan Kurtenbach, Abigail S. Hackam5, saline n = 8. Photoreceptor death The DeadEnd Fluorometric TUNEL system kit (Promega, catalog G3250) was used to quantify dying cells on ten-micron frozen retinal sections according to the manufacturer’s

(14) Tiki2 restrains Wnt5a/Ror signaling to limit inflammation in acute kidney injuryCommunications BiologyApril 14, 2026Cui Yang, Shujie Wang, Mei Zhang, Bryan T. MacDonald, Zheng Wang, Xi He, Rui Zeng, Xinjun Zhangstaining Apoptosis and pyroptosis in kidney sections were assessed via the DeadEnd™ Fluorometric TUNEL System (G3250, Promega, USA). After deparaffinization, hydration, and rinse, the sections were incubated with the

(15) Extracellular Vesicles Modulation by an Adiponectin Receptor Agonist Provides Cardioprotection for Myocardial Ischemic InjuryAdvanced Healthcare MaterialsApril 14, 2026Jialing Tang, Wing Yan Chung, Serena M. Pulente, Yubin Lei, Khang Nguyen, Eddie Tam, Nadya M. Morrow, Ilka Lorenzen‐Schmidt, Fengxia Xiao, Augusto Zani, et al.staining for 10 min at room temperature (Thermo, W11262) and followed with TUNEL staining (Promega, G3250) according to manufacturer's protocol. Sections cover with mounting medium contained DAPI (Vector Laboratories,

(16) Redox imbalance dictates dependence on GOT1 versus GOT2 for rod photoreceptor health during aging and stressbioRxivApril 7, 2026Meini Chen, Eric Weh, Moloy T. Goswami, Katherine M. Weh, Heather Hager, Peter Sajjakulnukit, Avi Weingarten, Shubha Subramanya, Nicholas Miller, Sraboni Chaudhury, et al.were washed and resuspended before staining using the DeadEnd Fluorometric TUNEL kit (Promega, Cat #G3250). Stained samples were analyzed using an Attune NXT flow cytometer (Thermo Fisher Scientific; FITC channel

(17) Pterostilbene attenuates lung ischemia-reperfusion injury: integrative insights from network pharmacology, molecular dynamics, and experimental validationFront PharmacolApril 1, 2026Haotian Bai, Heng Zhao, Jinteng Feng, Hongyi Wang, Yixing Li, Zhe Chen, Bin He, Chi Wang, Rui Gao, Rui Zhao, et al.gradient, embedded in paraffin, and sectioned at 4 µm. Sections were stained using a TUNEL assay kit (G3250, Promega, United States) following the manufacturer’s instructions and counterstained with DAPI. Apoptotic

(18) Transcription factor NFYA directs male meiotic entry by regulating accessible chromatin at meiotic promoters in miceThe EMBO JournalMarch 19, 2026Martin Säflund, Masomeh Askari, Atiyeh Eghbali, Mukhtar Mohamed Abdi, Dilay Deren Er, Ann-Kristin Östlund Farrants, Tianxiong Yu, Deniz M ÖzataNick-End Labeling (TUNEL) assay was performed using the DeadEnd Fluorometric TUNEL System (Promega; G3250) according to the manufacturer’s protocol. In all, 5-μm tissue sections were first deparaffinized in

(19) IncRNAs transcriptomics elucidates the potential mechanism of Naoshuantong capsule in alleviating synaptic dysfunction in a murine model of cerebral ischemia/reperfusion injuryFront PharmacolMarch 5, 2026Ke Song, Hongrui Zhang, Haoqi Liu, Yuanyuan Li, Yikun Sun, Xinglu Dong, Chenxi Tao, Yannan He, Zhenhong Liu, Yonghong Gao, et al.DeadEnd™ Fluorometric TUNEL System was sourced from Promega Corporation (Madison, Wisconsin, United States; Catalog Number: G3250). RIPA lysis buffer was procured from Beijing Pulilai Gene Technology Co., Ltd. (Beijing, China; Catalog

(20) NanoScript-Enabled Nonviral Transient Repression of Phosphatase and Tensin Homolog for Axonal Regeneration and Central Nervous System Injury RepairACS NanoMarch 3, 2026Brandon Conklin, Yanting Liu, Sarah Nevins, Byeong-Gwan Song, Sy-Tsong Dean Chueng, Qiu Xiaowen, Sungyun Kim, Heyin Cheung, Seong Bae An, JongMin Lee, et al.cells in the spinal cord tissue were detected using the DeadEnd Fluorometric TUNEL System (Cat. No. G3250; Promega, Madison, WI, USA) following the manufacturer’s protocol. Paraffin-embedded sections were deparaffinized

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8/11/2015Detecting and Quantifying Apoptotic Cells using DeadEnd™ Fluorometric TUNEL System Lenin VeeravalThis kit was used to detect and quantify the apoptotic cells in a cell population treated with anticancer compounds compared to control cells. The product is simple to use and has high throughput.

DeadEnd Fluorometric TUNEL System

DeadEnd Fluorometric TUNEL System from Promega

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DeadEnd Fluorometric TUNEL System from Promega

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