Flow Cytometry Buffers

Buffers for use in flow cytometry serve various purposes, including optimizing cellular staining, minimizing non-specific binding, and dilution. Flow cytometry-based cell sorting also employs such buffers. Buffers may contain sodium azide as a preservative and animal serum proteins (such as FBS or BSA) that minimize non-specific binding of antibodies. Buffers that help with cell permeability can comprise of PBS, saponin, and EDTA in addition to a serum protein. Some buffers can also include stains. Fixation buffers can contain paraformaldehyde, and help preserve the light-scattering characteristics and fluorescence staining of cells for use in flow cytometry after immunofluorescence analysis. Some flow cytometry buffers may also contain metabolic inhibitors and antimicrobials. Visit the supplier page for more product information such as quantity and format.