RBC Lysis Buffer (10X) from BioLegend

Product Specs
ItemRBC Lysis Buffer (10X)
CompanyBioLegend
Price $65.00Supplier Page View Company Product Page
Catalog Number420301
Quantity100 mL
FormatThis red blood cell (RBC) lysis buffer is supplied as a 10X solution and should be diluted to 1X in deionized water. 100 ml of 10X concentrate will yield a quantity of 1X solution that is sufficient to lyse 500 samples

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) DNA prime and peptide boost immunization elicits robust neoantigen-specific CD8 + T cell responses and therapeutic protection in mouse tumor modelsOncoImmunologyDecember 31, 2026Pablo Morgado-Cáceres, Francisca Hofmann-Vega, Diego Figueroa, Juan Saavedra-Almarza, Felipe Gálvez-Cancino, Ximena Díaz, Evelyn Menares, Eduardo Roa, Sofia Hidalgo, Manuel Varas-Godoy, et al.Red blood cells from spleen and blood samples collected were lysed using RBC lysis buffer (Biolegend 420301). Skin was cut into small fragments and digested in 1 mL non-supplemented RPMI 1640 medium containing

(2) Underlying MASLD-induced gut microbiome dysbiosis and intestinal inflammation are key to poor outcomes in vibriosis infections in a preclinical modelGut MicrobesDecember 31, 2026Punnag Saha, Subhajit Roy, Madhura More, Dipro Bose, Ayushi Trivedi, Bryan W. Brooks, Wing-Kin Syn, Anna Mae Diehl, Saurabh Chatterjeeg for 7 min at 4 °C to pellet down the NPCs, and 1x red blood cell (RBC) lysis buffer (Catalog No. 420301, BioLegend, San Diego, CA, USA) was added to remove any traces of erythrocytes. Finally, the isolated

(3) Bone targeted microenvironment actuated engineered exosomes for precision therapy of IBD associated bone lossBioactive MaterialsOctober 1, 2026Jiang Liu, Cong Wang, Taojin Feng, Siyu Chen, Siliang Ge, Yue Zhao, Ming Chen, Zhongqi Wang, Weier Bao, Pincong Fu, et al.out with sterile PBS, passed through a 70 μm cell strainer, and subjected to red blood cell lysis (420301, BioLegend). After washing, cells were resuspended in flow cytometry staining buffer. For Th17 analysis,

(4) Focused ultrasound thermal ablation and CD40 agonism reprograms breast tumor immunity to drive regression and memoryJ Immunother CancerAugust 4, 2026Zehra Ef Demir, Aeryon Kim, Beyzanur G Ak, Micaiah S Lee, Thomas Sherlock, Stefanyda O Maslova, Andrew T Thede, Matthew R DeWitt, Melanie Rutkowski, Natasha D Sheybanisamples were also subjected to hemolysis using BioLegend 1× red blood cell (RBC) lysis buffer (BioLegend #420301). Following RBC lysis, samples were quenched with complete media (RPMI or DMEM+10% FBS). Following,

(5) Pure Isolation, Culture, and Post-Injury Lineage Tracing of Mouse Visceral Pleural Mesothelial CellsAmerican Journal of Respiratory Cell and Molecular BiologyAugust 1, 2026Akikazu Endo, Yasunori Enomoto, Ryo Horiguchi, Shiori Meguro, Hideya Kawasaki, Isao Kosugi, Kenta Iijima, Shuji Takabayashi, Satoshi Baba, Toshihide IwashitaAfter centrifugation (400 g, 3 minutes), the cells were incubated with RBC lysis buffer (BioLegend, #420301) at room temperature for 3 minutes. Afterward, the cells were resuspended in DPBS with 3% FBS and centrifuged

(6) Bacteria-mimicking cancer cells reprogram macrophages via multiple pattern recognition receptor pathways for cancer immunotherapySignal Transduct Target TherJuly 27, 2026Seoyoon Song, Dongjun Yu, Haneul Kang, Deborah Lee, Jiye Lee, Sungjun Kwak, Jinhee Park, Lifeng Kang, Stanislav Y. Emelianov, Jong-Ho Cha, et al.syringes and strained with a 70 μm cell strainer. Red blood cells were lysed with RBC lysis buffer (#420301, BioLegend). The resulting cells were handled as described in the “Cells and cell culture” section. Cells

(7) Dysregulated myelopoiesis and enhanced glutaminase-dependent neutrophil survival in preclinical obesityImmunohorizonsJuly 25, 2026Madison Babcock, Aric Lechner, Julia Drolet, Lynn Agostini, Ruth Weinstock, Norifumi Uraodifferential blood cell counts. Whole blood cells were treated with 1X RBC lysis buffer (Biolegend, cat. no. 420301). Following lysis and thorough washing, blood monocytes were stained with CD45 (2D1), LIN—CD3, CD19,

(8) Increased Expression of HLA-DR and CD69 on Peripheral CD4+ T Cells Predicts Better Clinical Outcomes in Cutaneous MelanomaImmunotargets TherJuly 22, 2026Ana Tomás, José Maximino, Hugo Nunes, Rute Salvador, Rafael Luís, Cheila Brito, Diana P Saraiva, Emanuel Gouveia, Carolina Pereira, Filipe Gonçalves, et al.without brake). PBMCs were washed and red blood cells (RBC) lysed with RBC lysis buffer 1× (BioLegend, 420301) for 15 min at room temperature. After a final wash step, PBMCs were cryopreserved at 2×106 cells/mL

(9) Targeting the AHR-IDO1-kynurenine pathway in TREM2+ macrophages restores antitumor immunity in thyroid cancerFront ImmunolJuly 21, 2026Fei Le, Xianming Huang, Youfang Jiang, Saiping Lv, Yaoyao Chenfiltered through a 40 μm strainer (Falcon, 352340) and treated for red blood cell lysis (BioLegend, 420301). Cells were incubated with anti-mouse CD16/32 (Fc block; BioLegend, 101320) for 10 minutes, and cell

(10) Impact of high-fat Western diet on chronic lymphocytic leukemia disease progression and gut microbiome profile in Eµ-TCL1 miceFront OncolJuly 21, 2026Sydney A. Skupa, Jordan B. Hernandez, Audrey L. Smith, Erin M. Drengler, Jayesh Rai, Jianmin Pan, Anand K. Seth, Shesh N. Rai, Jonathan B. Clayton, Christopher R. D'Angelo, et al.fluorochrome-labeled antibodies at 4°C for 20 minutes followed by RBC lysis with 1X RBC Lysis Buffer (Cat. #420301; BioLegend; San Diego, CA, USA) per manufacturer protocol prior to flow cytometry acquisition. Fluorochrome

(11) Aripiprazole modulates splenic microbiota and immune response elicited by single prolonged stress in ratsPharmacol RepJuly 20, 2026Peter Vargovic, Andrej Tillinger, Lubica Horvathova, Lila Dziewiczova, Jana Osackacentrifuged for 10 min at 300 × g, 4 °C. Red blood cells were removed using red blood cells lysis buffer (#420301, BioLegend, USA) and twice washed. Samples were incubated at 37 °C for 3 h in the following experimental

(12) Single-Cell Dissection of Therapy-Induced Remodeling Uncovers a Fibroblast-Driven Immunosuppressive Niche and Targetable Vulnerabilities in Lethal Prostate CancerAdv Sci (Weinh)July 17, 2026Yang Chen, Dandan Dong, Jinling Liao, Naikai Liao, Mengqi Liu, Qin Zhang, Siting Chen, Qi Liu, Ying Lu, Tianyu Li, et al.disaggregation into single cells. Following digestion, 3 mL of 1× RBC lysis buffer (BioLegend, Cat. no. 420301) was applied to eliminate erythrocyte contamination. Cells were centrifuged, and the supernatant was

(13) Regulatory T cells establish an IL-10-IL10Rα immunometabolic checkpoint that limits HSL activation and lipolysisbioRxivJuly 13, 2026Ramazan Yildiz, Kajal Davi, Niki F. Brisnovali, James W.R. McMullen, Chung Hwan Cho, Khatanzul Ganbold, YoungUk Jang, Njeri Z.R. Sparman, Aidan Warnock, Gabriel Deards, et al.Pellets from blood and adipose tissue depots were resuspended in 1mL of RBC lysis buffer (BioLegend #420301) to lyse erythrocytes and incubated at room temperature for 10 minutes, and quenched with 9 mL of PBS.

(14) Fenofibrate alleviates the brain edema after traumatic brain injury by enhancing endothelial OXPHOSRedox BiologyJuly 1, 2026Qiyuan Feng, Yingwei Gao, Xiaokun Gu, Xinxin Zhao, Xinwen Liu, Zixuan Ma, Wenlan Qi, Zhenghui He, Yuhan Han, Jian Zhang, et al.400 × g for 25 min to enrich for microvascular cells. The pellet was treated with RBC lysis buffer (420301; BioLegend, USA), washed with PBS, and resuspended in staining buffer for downstream applications. For

(15) Therapeutic inhibition of telomeric DNA damage response rescues hematopoietic dysfunction driven by telomere shortening and agingNature AgingJune 30, 2026Alessia Oppezzo, Sara Sepe, Giada Cicio, Valeria Cancila, Emanuele Sasso, Anastasia Conti, Eugenia Marinelli, Matteo Cabrini, Rani Pallavi, Nicola Iacomino, et al.filters, followed by washing in PBS. RBCs were eliminated using RBC Lysis Buffer (BioLegend, cat. no. 420301), washed again in PBS, and frozen in freezing medium (90% fetal bovine serum, 10% dimethyl sulfoxide).

(16) Cigarette Smoke‐Induced Alveolar Macrophage Senescence via GEM/SIRT3‐Mediated Mitochondrial DysfunctionAdvanced ScienceJune 22, 2026Jin Wang, Meidan Wei, Xiangrong Song, Jiaxin Zhang, Lisha Zhang, Sixian Chen, Yaoyu Hu, Hui Gao, Jianxiang Lisupernatant was discarded. Red blood cells were removed via red blood cell lysis buffer (BioLegend, #420301). After centrifugation, the pellet was resuspended in 1 mL of phosphate‐buffered saline (PBS). Cell counts

(17) PERK orchestrates an endoplasmic reticulum stress alternative splicing program via CLK1/SRSF1Nature CommunicationsJune 19, 2026Céline Philippe, Shoshana Burke, Arantxa Carrasco-Leon, Andrea Martisova, Pedro Casado, Eleni Maniati, Jimena Castorena, Pantelitsa Protopapa, Alyssa Fronk, Ru-Pin Alicia Chi, et al.Ficoll-Paque density centrifugation method (GE Healthcare Life Sciences) and red blood cell lysis (BioLegend, #420301). Following CD34+ cells selection by magnet (StemCell Tech, #17898), the CD34- fraction was kept and

(18) Induction of broadly neutralizing HIV antibodies by a two-step mechanism informs vaccine designScienceJune 18, 2026Ashwin N. Skelly, Harry B. Gristick, Hui Li, Edem Gavor, Andrew J. Connell, Edward F. Kreider, Lorie Marchitto, Michael P. Hogarty, Maddy L. Newby, Joel D. Allen, et al.°C. If heavy RBC contamination was observed, lysis was performed using RBC Lysis Buffer (Biolegend 420301). The cell pellet was resuspended in 30 mL of HBSS with Mg2+/Ca2+ and 1% FBS, and cell count and viability

(19) Improved expansion of peripheral blood and iPSC-derived natural killer cells for clinical applicationsCell Reports MethodsJune 15, 2026Jaya Lakshmi Thangaraj, Edith Lopez, Dan S. KaufmanC34571 CTS NK-Xpander Medium,Life Technologies,Cat# A5019001 RBC Lysis Buffer (10X),Biolegend,Cat# 420301 ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, EasySep Human NK Cell Enrichment Kit

(20) Comparative effects of platelets and plasma-derived mitochondria in early-phase Achilles tendon inflammationRegenerative BiomaterialsJune 13, 2026Seong-Hoon Kim, Eun-Seo Back, Mina Lim, Mi Jin Kim, Chang-Koo Yun, Yong-Soo Choistrainer to remove undigested debris. Red blood cells were removed using RBC lysis buffer (BioLegend, 420301). An aliquot of the resulting cells was reserved for flow cytometry, and the remaining cells were used

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RBC Lysis Buffer (10X) from BioLegend

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RBC Lysis Buffer (10X) from BioLegend

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