RBC Lysis/Fixation Solution (10X) from BioLegend

Product Specs
ItemRBC Lysis/Fixation Solution (10X)
CompanyBioLegend
Price $141.00Supplier Page View Company Product Page
Catalog Number422401
Quantity50 mL
ApplicationsFC - Quality tested
FormatThis RBC Lysis/Fixation Solution is supplied as a 10X solution and should be diluted to 1X in room temperature deionized water

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) HIF-2α accumulation in human monocytes upon transfer of hypoxia-associated miRNAs via plasma-derived small extracellular vesicles from head and neck cancer patientsFrontiers in OncologyJanuary 12, 2026Marie-Nicole Theodoraki, Linda Hofmann, Helen Bauer, Jonas Fleckner, Reinhard Depping, Christian Idel, Kirstin Plötze-Martin, Zuzana Penxova, Dirk Rades, Thomas K. Hoffmann, et al.BioLegend, San Diego, CA, USA). After 25-min staining in the dark, 650 µL RBC Lysis/Fixation Buffer (Cat: 422401; BioLegend, San Diego, CA, USA) was added to the samples and incubated for another 20 min. Subsequently,

(2) Parallel Evolution of Leukemic Clones in Myeloproliferative NeoplasmsResearch SquareNovember 17, 2025Tyler M. Challen, Aishwarya Krishnan, Infencia Xavier Krishnan, Infencia Xavier Young, David R. O'Leary, David O'Leary, Maggie Arand, Stephen T. Allen, Grant A. Challensurface marker staining, whole BM was lysed and fixed with RBC Lysis/Fixation Solution (BioLegend #422401) at 37C for 15 minutes and subsequent permeabilization with True Phos Perm Buffer (BioLegend #425401)

(3) A disrupted adenosinergic axis facilitates platelet activation in APS: exploring a novel therapeutic targetBlood AdvancesNovember 11, 2025Somanathapura K. NaveenKumar, Thalia G. Newman, Bruna Mazetto Mazetto Fonseca, Srilakshmi Yalavarthi, Mariane C. Flores Nascimento, Kaitlyn Sabb, Katarina Kmetova, Emily Chong, Kavya Sugur, Caroline H. Ranger, et al.remaining cells were fixed using the red blood cell lysis/fixation solution (BioLegend, catalog no. 422401). After centrifugation, cells were washed and analyzed with a BioRad-ZE5 flow cytometer. FlowJo software

(4) Humanization of CD47 enables development of functional human neutrophils via postirradiation remodeling of the bone marrowProceedings of the National Academy of SciencesSeptember 23, 2025Esen Sefik, William Philbrick, Fengrui Zhang, Kriti Agrawal, Brian Van Lee, Johannes Sam, Kutay Karatepe, Yunjiang Zheng, Kaixin Liang, Sophia Peng, et al.cocktail at room temperature for 20 min and then treated with RBC lysis/fixation solution (Biolegend #422401). Fixed cells were then washed and resuspended with PBS for analysis. BMs (femurs) were dissected and

(5) Evaluation of the podoplanin/C-type lectin-like receptor-2 (CLEC-2) pathway as a mediator of platelet and coagulation activation in sickle cell diseaseResearch and Practice in Thrombosis and HaemostasisSeptember 1, 2025Ivanio Teixeira Borba-Junior, Mayck da Silva Barbosa, Carla Roberta Peachazepi Moraes, Letícia Queiroz Silva, Irene Santos, Bruno Benites, Joyce M. Annichino-Bizzacchi, Fernando Ferreira Costa, Erich Vinicius De Paulalight, red blood cells were lysed using a lysing and fixing solution for 15 minutes (BioLegend cat. 422401). Samples were centrifuged (540g, 5 minutes), the supernatant was discarded, and the cells were then

(6) Gut microbiota and SCFAs improve the treatment efficacy of chemotherapy and immunotherapy in NSCLCNPJ Biofilms MicrobiomesJuly 28, 2025Yanping Yang, Maosong Ye, Yijun Song, Wenyu Xing, Xing Zhao, Yufan Li, Jiacheng Shen, Jian Zhou, Kinji Arikawa, Shengdi Wu, et al.mixture was incubated at room temperature in the dark for 20 min. 10× red blood cell lysate (BioLegend, 422401) was prepared into a 1× working solution with deionized water and equilibrated at room temperature before

(7) The TLR7/8 agonist INI-4001 enhances the immunogenicity of a Powassan virus-like-particle vaccineNPJ VaccinesJuly 16, 2025Michael W. Crawford, Walid M. Abdelwahab, Karthik Siram, Christopher J. Parkins, Henry F. Harrison, E. Taylor Stone, Samantha R. Osman, Dillon Schweitzer, David J. Burkhart, Amelia K. Pinto, et al.at room temperature. Cells were then washed and fixed using RBC lysis/fixation solution (BioLegend #422401) for 10 min, washed, and resuspended in FACS buffer. Cells were then analyzed by flow cytometry using

(8) Biologically targeted dual adaptive and innate nano-Immunotherapy for clear cell renal cell carcinoma treatmentMol CancerJune 18, 2025Kin Man Au, Siqing Li, Tian Zhang, Andrew Z. Wangstained cells were fixed, and red blood cells were lysed by RBC Lysis/Fixation solution (catalog number 422401, BioLegend) according to the manufacturer’s protocol. Fixed cells were analyzed in a BD LSR II flow cytometer

(9) A Participant-Derived Xenograft Mouse Model to Decode Autologous Mechanisms of HIV Control and Evaluate ImmunotherapiesBio ProtocApril 5, 2025Emma Falling Iversen, Itzayana G. Miller, Ole Søgaard, Ali Danesh, Brad R. Joneslaser/filter configurations 21. RBC lysis buffer/fixation solution (10×) (BioLegend, catalog number: 422401) 22. Hanks balanced salt solution (HBSS) (Gibco, catalog number: 14025076) 23. DMSO (Thermo Scientific

(10) A Participant-Derived Xenograft Mouse Model to Decode Autologous Mechanisms of HIV Control and Evaluate ImmunotherapiesBIO-PROTOCOLApril 5, 2025Emma Falling Iversen, Itzayana G. Miller, Ole Søgaard, Ali Danesh, Brad R. Joneslaser/filter configurations 21. RBC lysis buffer/fixation solution (10×) (BioLegend, catalog number: 422401) 22. Hanks balanced salt solution (HBSS) (Gibco, catalog number: 14025076) 23. DMSO (Thermo Scientific

(11) The TLR7/8 agonist INI-4001 enhances the immunogenicity of a Powassan virus-like-particle vaccinebioRxivDecember 6, 2024Michael W. Crawford, Walid M. Abdelwahab, Karthik Siram, Christopher J. Parkins, Henry F. Harrison, Samantha R. Osman, Dillon Schweitzer, Jay T. Evans, David J. Burkhart, Amelia K. Pinto, et al.at room temperature. Cells were then washed and fixed using RBC lysis/fixation solution (BioLegend #422401) for 10 minutes, washed, and resuspend in FACS buffer. Cells were then analyzed by flow cytometry using

(12) Unveiling novel insights into human IL-6 - IL-6R interaction sites through 3D computer-guided docking and systematic site mutagenesisScientific reportsAugust 7, 2024Li K, Cai J, Jiang Z, Meng Q, Meng Z, Xiao H, Chen G, Qiao C, Luo L, Yu J, et al.ng/mL) for 15 min at 37 °C. Then, 2 mL of cold red blood cell lysing/fixation buffer (Biolegend, Cat. 422401) was added to lyse the erythrocytes and fix the phosphorylated status of STAT3, followed by incubation

(13) Parallelized Immunomagnetic Isolation of Basophils Directly from Whole BloodAdvanced NanoBiomed ResearchJuly 15, 2024Justin Myles, Nicolas Castaño, Sungu Kim, Zhenyun Zhu, Sindy K.Y. Tangexperiments to measure purity, we fixed and lysed the cells using a 1x RBC lysis/fixation solution (catalog #422401, BioLegend). 900 μL of the fix/lyse solution was added to each sample and incubated for 30 minutes at

(14) Vpr attenuates antiviral immune responses and is critical for full pathogenicity of SIV(mac239) in rhesus macaquesiScienceDecember 15, 2023Laliberté A, Prelli Bozzo C, Stahl-Hennig C, Hunszinger V, Joas S, Sauermann U, Roshani B, Klippert A, Daskalaki M, Mätz-Rensing K, et al.DNA Library Preparation Kit,Illumina,Cat#FC-131-1096 RBC Lysis/Fixation Solution (10X),Biolegend,Cat#422401 FOXP3 Fix/Perm Buffer Set,Biolegend,Cat#421403 Rhesus Macaque BLC ELISA Kit,Invitrogen,Cat#EP3RB TaKaRa

(15) BRISC is required for optimal activation of NF-κB in Kupffer cells induced by LPS and contributes to acute liver injuryCell Death & DiseaseNovember 15, 2023Wen Zhang, Kai Liu, Guang-Ming Ren, Yu Wang, Ting Wang, Xian Liu, Dong-Xu Li, Yang Xiao, Xu Chen, Ya-Ting Li, et al.CD11b-eFluor 450. Red blood cells (RBCs) were then lysed using RBC Lysis/Fixation Solution (Biolegend, 422401). Murine liver leukocytes were isolated with a modification method [56]. In brief, after the two-step

(16) The effect of chronic kidney disease on tissue formation of in situ tissue-engineered vascular graftsAPL bioengineeringJune 1, 2023Besseling PJ, Krebber MM, Fledderus JO, Teraa M, den Ouden K, van de Kaa M, de Bree PM, Serrero A, Bouten CVC, Dankers PYW, et al.and age matched Sham animals. Red blood cells were lysed with RBC Lysis/Fixation Solution (Biolegend #422401). Fixed cells were labeled with the following antibodies; anti-RP1-BV421 (1:100, BD Biosciences #743053),

(17) NLRP12-expressing dendritic cells mediate both dissemination of infection and adaptive immune responses in visceral leishmaniasisiScienceMarch 17, 2023Valadares DG, Clay OS, Chen Y, Scorza BM, Cassel SL, Sutterwala FS, Wilson ME.P8139 Permeabilization Buffer (10X),Thermofisher,Cat# 00-8333-56 RBC Lysis/Fixation Solution (10X),Biolegend,Cat# 422401 Recombinant mouse CCL21,PeproTech,Cat# 250-13 Recombinant mouse CCL19,PeproTech,Cat# 250-27B Recombinant

(18) Rapid and robust flow cytometric method to analyze neutrophil functions in human and mouse whole blood samplesJournal of Immunological MethodsJanuary 1, 2023Carola Ledderose, Naoyuki Hashiguchi, Eleftheria-Angeliki Valsami, Christian Rusu, Wolfgang G. JungernM W-peptide,Tocris,1800,HBSS,0.5 mM,HBSS,0.125-25 μM,0.05-10 μM RBC Lysis/Fixation Solution,BioLegend,422401,DI H2O,10x,-,1x, Sources of key reagents and preparation of stock and working solutions

(19) The alterations of circulating mucosal-associated invariant T cells in polycystic ovary syndromeFront Endocrinol (Lausanne)November 28, 2022Hong Zhou, Junting Xu, Ling Hong, Yanping Jia, Lilo Valerie Burk, Fengli Chi, Mei Zhao, Xiaohong Guan, Dan Liu, Xiangjie Yin, et al.the dark for 20 minutes. Cell lysis and fixation was performed with 1x RBC Lysis/Fixation Solution (422401, BioLegend, San Diego, USA) for 15 minutes at room temperature in the dark. After centrifuging at 350g

(20) Human neutrophil development and functionality are enabled in a humanized mouse modelProceedings of the National Academy of Sciences of the United States of AmericaOctober 25, 2022Zheng Y, Sefik E, Astle J, Karatepe K, Öz HH, Solis AG, Jackson R, Luo HR, Bruscia EM, Halene S, et al.temperature for 20 min and then treated with red blood cell (RBC) lysis/fixation solution (BioLegend #422401). Fixed cells were then washed and resuspended with PBS supplemented with 2% FBS and 1 mM EDTA (FACS

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RBC Lysis/Fixation Solution (10X) from BioLegend

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RBC Lysis/Fixation Solution (10X) from BioLegend

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