pRL-TK Vector from Promega

Product Specs
ItempRL-TK Vector
CompanyPromega
Price $0.00Supplier Page View Company Product Page
Catalog NumberE2241
HostMammalian
PromoterHSV TK promoter
Vector NamepRL-TK
Quantity20microg
ReporterRenilla luciferase
ResistanceAmpR
Molecular Weight~73kDa
Selectable MarkerAmpicillin

Specifications/Features

Vector Size:
4045 bp

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(161) Repression of rRNA gene transcription by endothelial SPEN deficiency normalizes tumor vasculature via nucleolar stressJ Clin InvestOctober 16, 2023Zi-Yan Yang, Xian-Chun Yan, Jia-Yu-Lin Zhang, Liang Liang, Chun-Chen Gao, Pei-Ran Zhang, Yuan Liu, Jia-Xing Sun, Bai Ruan, Juan-Li Duan, et al.transfected with 200 ng of the p53 reporter plasmid (p53-luc, Yeasen, 11540ES03) and 10 ng pRL-TK (Promega, E2241). The cells were harvested 24 hours after transfection, and the luciferase activity was analyzed with

(162) Identification of miR-128 Target mRNAs That Are Expressed in B Cells Using a Modified Dual Luciferase VectorBiomoleculesOctober 13, 2023Sandra Schreiber, Patrick Daum, Heike Danzer, Manuela Hauke, Hans-Martin Jäck, Jürgen Wittmannfragments were inserted into the single luciferase vector pRL-TK (Promega, Mannheim, Germany, Cat. No. E2241) or the dual luciferase vector psiCHECK-4 (this work) by restriction digest cloning (all restriction

(163) Remote regulation of rs80245547 and rs72673891 mediated by transcription factors C-Jun and CREB1 affect GSTCD expressioniScienceAugust 18, 2023Jin-Xiu Li, Xue-Zhen Huang, Wei-ping Fu, Xiao-hua Zhang, David H. Mauki, Jing Zhang, Chang Sun, Lu-Ming Dai, Li Zhong, Li Yu, et al.Scientific,RPCI11.C pGL3-Basic Vector,Promega Corporation,E1751 pRL-TK Vector,Promega Corporation,E2241 pSpCas9(BB)-2A-GFP (PX458),Addgene,48138 ,, Biological samples,Biological samples,Biological samples ,, Human

(164) Integrative omics reveals rapidly evolving regulatory sequences driving primate brain evolutionMolecular Biology and EvolutionAugust 3, 2023Xiao-Lin Zhuang, Jin-Jin Zhang, Yong Shao, Yaxin Ye, Chun-Yan Chen, Long Zhou, Zheng-bo Wang, Xin Luo, Bing Su, Yong-Gang Yao, et al.pGL3-promoter vector (Promega, Cat. # E1761) and cotransfected with the internal control vector pRL-TK (Promega, Cat. #E2241) into mouse cell line N2A or human cell lines HEK293T and SH-SY5Y. Cells were harvested after 48 h,

(165) Aicardi Syndrome Is a Genetically Heterogeneous DisorderGenesJuly 31, 2023Ha TT, Burgess R, Newman M, Moey C, Mandelstam SA, Gardner AE, Ivancevic AM, Pham D, Kumar R, Smith N, et al.from Randall Moon [44]. Renilla luciferase vector, pRL-TK plasmid, was obtained from Promega (Cat #E2241, Promega, Alexandria, Australia). The WNT8B p.L70P vector was cloned using a PCR insert, with a mutant

(166) HSPB1 facilitates chemoresistance through inhibiting ferroptotic cancer cell death and regulating NF-κB signaling pathway in breast cancerCell Death & DiseaseJuly 15, 2023Yiran Liang, Yajie Wang, Yan Zhang, Fangzhou Ye, Dan Luo, Yaming Li, Yuhan Jin, Dianwen Han, Zekun Wang, Bing Chen, et al.(11501ES03, Shanghai, China). The NF-κB reporter vector and reference Renilla luciferase vector (Promega, E2241, Madison, Wisconsin, USA) were co-transfected into breast cancer cells at a ratio of 10:1. At 48 h post-transfection,

(167) Systematic characterization of regulatory variants of blood pressure genesCell GenomicsJuly 12, 2023Winona Oliveros, Kate Delfosse, Daniella F. Lato, Katerina Kiriakopulos, Milad Mokhtaridoost, Abdelrahman Said, Brandon J. McMurray, Jared W.L. Browning, Kaia Mattioli, Guoliang Meng, et al.paper,N/A ,, Recombinant DNA,Recombinant DNA,Recombinant DNA ,, pGL4.29,Promega,Cat#E8471 pRL-TK,Promega,Cat#E2241 MPRA_backbone_empty_pGL4-2.3_cloning_site_polyA,Mattioli et al.21,N/A pCMV-PE2,Anzalone et al.88,Addgene

(168) SELENOP modifies sporadic colorectal carcinogenesis and WNT signaling activity through LRP5/6 interactionsJ Clin InvestJuly 3, 2023Jennifer M. Pilat, Rachel E. Brown, Zhengyi Chen, Nathaniel J. Berle, Adrian P. Othon, M. Kay Washington, Shruti A. Anant, Suguru Kurokawa, Victoria H. Ng, Joshua J. Thompson, et al.M50 Super 8x TOPFlash reporter plasmid (12456, Addgene) and 0.05 μg pRL-TK control reporter plasmid (E2241, Promega) using Lipofectamine 2000. Forty-eight hours later, cells were treated with or without WNT3A

(169) SOX17-mediated LPAR4 expression plays a pivotal role in cardiac development and regeneration after myocardial infarctionExperimental & molecular medicineJuly 1, 2023Lee JW, Lee CS, Son H, Lee J, Kang M, Chai J, Cho HJ, Kim HS.reporter vector (E1751, Promega, Madison, WI, USA) and the Renilla luciferase reporter plasmid pRL-TK (E2241, Promega) using the FuGENE® HD transfection reagent (E2311, Promega). The Dual-Glo® Luciferase Assay system

(170) A novel αB-crystallin R123W variant drives hypertrophic cardiomyopathy by promoting maladaptive calcium-dependent signal transductionFrontiers in Cardiovascular MedicineJune 26, 2023Chun Chou, Gregory L. Martin, Gayani Perera, Junya Awata, Amy Larson, Robert Blanton, Michael T. Chintranscriptional activity in vitro, a mixture of 9X NFAT-alpha-MHC-Luc (51,941, Addgene) and pRL-TK (E2241, Promega) plasmids at a ratio of 3:1 was transfected into H9c2 cell lines expressing human wild-type

(171) Integrated analysis of genome-wide association studies and 3D epigenomic characteristics reveal the BMP2 gene regulating loin muscle depth in Yorkshire pigsPLOS GeneticsJune 20, 2023Yuanxin Miao, Yunxia Zhao, Siqi Wan, Quanshun Mei, Heng Wang, Chuanke Fu, Xinyun Li, Shuhong Zhao, Xuewen Xu, Tao Xiang48-well plates and co-transfected with reporter vectors and pRL-TK renilla luciferase control vector (E2241, Promega) using Lipofectamine 2000 (11668500, Invitrogen). The transfected cells were incubated for

(172) Krüppel-like factor 10 modulates stem cell phenotypes of pancreatic adenocarcinoma by transcriptionally regulating notch receptorsJournal of biomedical scienceJune 12, 2023Tsai YC, Cheng KH, Jiang SS, Hawse JR, Chuang SE, Chen SL, Huang TS, Ch'ang HJ.S3A, B). Cells were transfected with individual reporter constructs and co-transfected with pRLTK (E2241, Promega), which constitutively expresses renilla luciferase, to normalize transfection efficiency.

(173) A microRNA Arising from the Negative Strand of SARS-CoV-2 Genome Targets FOS to Reduce AP-1 ActivityNon-Coding RNAMay 23, 2023Francesco Greco, Elisa Lorefice, Claudia Carissimi, Ilaria Laudadio, Fabiola Ciccosanti, Martina Di Rienzo, Francesca Colavita, Silvia Meschi, Fabrizio Maggi, Gian Maria Fimia, et al.(HCT116) or 100 ng (HEK293T) 3xAP1pGL3 reporter and 25 ng (HCT116) or 10 ng (HEK293T) pRL-TK (Promega E2241) plasmid were transfected using Jetprime transfection reagent (Polyplus 115). Eighteen hours later renilla

(174) Ctnnb1/β-catenin inactivation in UCP1-positive adipocytes augments the browning of white adipose tissueiScienceMay 19, 2023Chen N, Yuan M, Zhang N, Chen M, Liu R, Wang J, Lu P.pGL4.12,Promega,Cat#E6671 Plasmid: pGL4.12-UCP1 promoter,This paper,N/A Plasmid: pRL-TK,Promega,Cat#E2241 ,, Software and algorithms,Software and algorithms,Software and algorithms ,, GraphPad Prism (v9.0.0),GraphPad,http://www.graphpad.com/scientific-software/prism/ SPSS

(175) miR‑4732‑5p promotes ovarian cancer mobility by targeting MCUR1Oncology LettersApril 20, 2023Xin Li, Xiaoqin Wang, Jun Wu“CUCUACA” and corresponding mutation with sequences of “AGAGCAG”, 26 ng of pRL-TK plasmids (cat. no. E2241, Promega Corporation) containing Renilla luciferase, along with 10 pmol/ml miR-4732-5p mimics or negative

(176) Genetic regulatory and biological implications of the 10q24.32 schizophrenia risk locusBrainApril 19, 2023Junyang Wang, Jiewei Liu, Shiwu Li, Xiaoyan Li, Jinfeng Yang, Xinglun Dang, Changgai Mu, Yifan Li, Kaiqin Li, Jiao Li, et al.respectively. The recombinant vectors (150 ng) and the pRL-TK (internal control, 50 ng, Promega, Cat. No. E2241) were then co-transfected into three cell lines (HEK293T, SK-N-SH and SH-SY5Y) using the LipofectamineTM

(177) Single Nucleotide Polymorphism rs9277336 Controls the Nuclear Alpha Actinin 4‐Human Leukocyte Antigen‐DPA1 Axis and Pulmonary Endothelial Pathophenotypes in Pulmonary Arterial HypertensionJournal of the American Heart AssociationApril 4, 2023Neha Hafeez, Anna Kirillova, Yunshan Yue, Rashmi J. Rao, Neil J. Kelly, Wadih El Khoury, Yassmin Al Aaraj, Yi‐Yin Tai, Adam Handen, Ying Tang, et al.amount of the vector pRL‐TK that provides constitutive expression of Renilla luciferase (Catalog #: E2241, Promega). The firefly luciferase reporter activity was measured by normalizing the firefly luciferase

(178) Single Nucleotide Polymorphism rs9277336 Controls the Nuclear Alpha Actinin 4‐Human Leukocyte Antigen‐DPA1 Axis and Pulmonary Endothelial Pathophenotypes in Pulmonary Arterial HypertensionJ Am Heart AssocMarch 28, 2023Neha Hafeez, Anna Kirillova, Yunshan Yue, Rashmi J. Rao, Neil J. Kelly, Wadih El Khoury, Yassmin Al Aaraj, Yi‐Yin Tai, Adam Handen, Ying Tang, et al.amount of the vector pRL‐TK that provides constitutive expression of Renilla luciferase (Catalog #: E2241, Promega). The firefly luciferase reporter activity was measured by normalizing the firefly luciferase

(179) PHF6 recruits BPTF to promote HIF-dependent pathway and progression in YAP-high breast cancerJournal of Translational MedicineMarch 26, 2023Sheng Gao, Wensheng Zhang, Jingjing Ma, Xiaojian Niwith 200 ng of YAP/YAP-mutant construct and 10 ng of Renilla luciferase pRL-TK plasmid (Promega, Cat.#E2241). After 48 h, the dual luciferase assay was performed using the Dual-Luciferase® Reporter Assay System

(180) Identification of Potentially Functional Circular RNA/Long Noncoding RNA-MicroRNA-mRNA Regulatory Networks Associated with Vascular Injury in Type 2 Diabetes Mellitus by Integrated Microarray AnalysisJournal of Diabetes ResearchMarch 10, 2023Yi Leng, Ming-zhu Wang, Kang-ling Xie, Ying Caibioinformatically predicted miR-92a-2-5p to target genes. With pRL-TK vector expressing Renilla luciferase (E2241, Promega) as an internal reference, miR-92a-2-5p mimic and negative control were cotransfected with

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pRL-TK Vector

pRL-TK Vector from Promega

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Specifications/Features

Promega

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pRL-TK Vector from Promega

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