pRL-TK Vector from Promega

Product Specs
ItempRL-TK Vector
CompanyPromega
Price $0.00Supplier Page View Company Product Page
Catalog NumberE2241
HostMammalian
PromoterHSV TK promoter
Vector NamepRL-TK
Quantity20microg
ReporterRenilla luciferase
ResistanceAmpR
Molecular Weight~73kDa
Selectable MarkerAmpicillin

Specifications/Features

Vector Size:
4045 bp

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(121) The molecular consequences of FOXF1 missense mutations associated with alveolar capillary dysplasia with misalignment of pulmonary veinsJournal of Biomedical ScienceNovember 4, 2024G. G. Edel, M. van Kempen, A. Boerema-de Munck, C. N. Huisman, C. A. P. Naalden, R. W. W. Brouwer, S. Koornneef, W. F. J. van IJcken, R. M. H. Wijnen, R. J. Rottierexpression plasmid, 50 ng of pGL4.10[luc2] reporter plasmid and 10 ng of TK-Renilla plasmid (Promega; E2241). Luciferase activity was measured 24 h after transfection using the Dual-Luciferase Reporter Assay System

(122) Rap1A Modulates Store-Operated Calcium Entry in the Lung Endothelium: A Novel Mechanism Controlling NFAT-Mediated Vascular Inflammation and PermeabilityArteriosclerosis, Thrombosis, and Vascular BiologyNovember 1, 2024Ramoji Kosuru, Olivier Romito, Guru Prasad Sharma, Francesca Ferraresso, Behshid Ghadrdoost Nakhchi, Kai Yang, Tadanori Mammoto, Akiko Mammoto, Christian J. Kastrup, David X. Zhang, et al.hours, these cells were further transfected with a Renilla luciferase plasmid (pRL TK; Promega; No. E2241) alone or together with a plasmid encoding NFAT luciferase reporter (Addgene; No. 10959). Following

(123) Possible involvement of a MEG3-miR-21-SPRY1-NF-κB feedback loop in spermatogenic cells proliferation, autophagy, and apoptosisiScienceOctober 18, 2024Xingyu Fang, Xiaotong Lu, Yujie Ma, Ning Sun, Yunyun Jiao, Hui Meng, Mengjiao Song, Haixia Jin, Guidong Yao, Ning Song, et al.DNA,Recombinant DNA ,, pcDNA3.1-MEG3,Synbio technologies,N/A psiCHECK™ -2,Promega,C8021 pRL-TK,Promega,E2241 pGL3-basic,Promega,N/A pcDNA3.1-SPRY1,pcDNA3.1-MEG3,N/A ,, Software and algorithms,Software and

(124) 3-hour genome sequencing and targeted analysis to rapidly assess genetic riskGenetics in Medicine OpenOctober 18, 2024Miranda P.G. Zalusky, Jonas A. Gustafson, Stephanie C. Bohaczuk, Ben Mallory, Paxton Reed, Tara Wenger, Erika Beckman, Irene J. Chang, Cate R. Paschal, Jillian G. Buchan, et al.GCAGAGCTCTGTGACTGGCTGCTGGAGATGC 3’). The pRL:TK renilla luciferase plasmid is commercially available (Promega #E2241). All plasmids were validated by Sanger sequencing (Genewiz). We then transformed the pGL3:SLC39A4/T

(125) Long non-coding RNA Malat1 is essential for fine-tuning bone homeostasis through orchestrating cellular crosstalk and the β-catenin-OPG/Jagged1 pathwayResearch SquareOctober 12, 2024Baohong Zhao, Yongli Shirakawa, Jumpei Shirakawa, Cheng Chen, Courtney Chen, Courtney Nakano, Shinichi Elguindy, Zhonghao Elguindy, Kannanganattu V Prasanth, Kannanganattu Prasanth, et al.plasmid of M50 Super 8x TOPFlash (Cat# 12456) was purchased from Addgene. pRL-Tk control plasmid (Cat# E2241) was purchased from Promega. Fetal Bovine Serum (FBS, Cat #S11550) was obtained from Atlanta Biologicals.

(126) Rap1A Modulates Store-Operated Calcium Entry in the Lung Endothelium: A Novel Mechanism Controlling NFAT-Mediated Vascular Inflammation and PermeabilityArterioscler Thromb Vasc BiolSeptember 26, 2024Ramoji Kosuru, Olivier Romito, Guru Prasad Sharma, Francesca Ferraresso, Behshid Ghadrdoost Nakhchi, Kai Yang, Tadanori Mammoto, Akiko Mammoto, Christian J. Kastrup, David X. Zhang, et al.hours, these cells were further transfected with a Renilla luciferase plasmid (pRL TK; Promega; No. E2241) alone or together with a plasmid encoding NFAT luciferase reporter (Addgene; No. 10959). Following

(127) Protocol for linking enhanced interferon immunity to virus resistance in gene-knockout zebrafishSTAR ProtocolsSeptember 20, 2024Zi-Ling Qu, Yi-Bing ZhangDNA ,, pcDNA3.1(+),Invitrogen,Cat# V79020 pUC19-gRNA,Addgene,137776 pRL-TK (expression plasmid),Promega,Cat# E2241 DrIFNφ1pro-luc (expression plasmid),Sun et al.11,N/A TBK1 (expression plasmid),Qu et al.1,N/A IRF7

(128) Birth of protein folds and functions in the viromeNatureSeptember 19, 2024Jason Nomburg, Erin E. Doherty, Nathan Price, Daniel Bellieny-Rabelo, Yong K. Zhu, Jennifer A. Doudnadriven by an IFNB promoter (IFN-Beta_pGL3, Addgene 102597), 5 ng Renilla luciferase (pRL-TK, Promega E2241), and 20 ng of each putative PDE using the Mirus TransITX2 transfection reagent. After at least 4 h,

(129) Inter3D: Capture of TAD Reorganization Endows Variant Patterns of Gene TranscriptionGenomics, Proteomics & BioinformaticsSeptember 13, 2024Tianyi Ding, Shaliu Fu, Xiaoyu Zhang, Fan Yang, Jixing Zhang, Haowen Xu, Jiaqi Yang, Chaoqun Chen, Yibing Shi, Yiran Bai, et al.employed by introducing 900 ng of pGL3-E1/E2-promoter or pGL3-promoter and 90 ng of pRL-TK (Catalog No. E2241, Promega) into HEK293T cells using Lipofectamine 3000 (Catalog No. 18324012, Invitrogen). After 48 h,

(130) Identification of zinc finger MIZ-type containing 2 as an oncoprotein enhancing NAD-dependent protein deacetylase sirtuin-1 deacetylase activity to regulate Wnt and Hippo pathways in non-small-cell lung cancerCellular & Molecular Biology LettersSeptember 12, 2024Xueting Gan, Yuheng Feng, Yang Liu, Xuyong Lin, Xinmiao Yu, Xuezhu Rong, Qiang Han× Topflash (no. 12456) plasmids were purchased from Addgene (Cambridge, MA). The pRL-TK vector (no. E2241) was purchased from Promega (Madison, Wisconsin). HA-TCF4 (NM_001083962), HA-TEAD (NM_021961), pEGFP-N1

(131) Plasma exosomes carrying mmu-miR-146a-5p and Notch signalling pathway–mediated synaptic activity in schizophreniaJournal of Psychiatry and NeuroscienceAugust 29, 2024Zhichao Wang, Tong Wu, Houjia Hu, Alabed Ali A. Alabed, Guangcheng Cui, Lei Sun, Zhenghai Sun, Yuchen Wang, Ping Liwild-type and mutant NOTCH1 (NOTCH1-WT and NOTCH1-MUT) sequences into the pRL-TK reporter plasmid (E2241, Promega Corporation). We cultured 293T cells (CRL-3216, ATCC, https://www.atcc.org) in 96-well plates,

(132) Lipases are differentially regulated by hormones to maintain free fatty acid homeostasis for insect brain developmentBMC BiologyAugust 13, 2024Yan-Xue Li, Qiao Yan, Tian-Wen Liu, Jin-Xing Wang, Xiao-Fan Zhaocontaining target promoter driving firefly luciferase (Fluc), the reference pRL-TK plasmid (Promega, E2241) containing HSV-TK promoter driving renilla-luciferase (Rluc). The promoter of the HSV-TK in pRL-TK

(133) Conserved Antagonization of Type I Interferon Signaling by Arterivirus GP5 ProteinsVirusesAugust 1, 2024Rissar Siringo Ringo, Amonrat Choonnasard, Tamaki Okabayashi, Akatsuki Saito20 March 2024; RRID:Addgene_32713) [31]. The pRL-TK plasmid is commercially available (Promega, Cat# E2241). 2.2. Construction of Plasmids Encoding Myc-Tagged Pig TRIF Protein To generate a pCAGGS vector encoding

(134) Human cytomegalovirus UL23 exploits PD-L1 inhibitory signaling pathway to evade T cell-mediated cytotoxicitymBioJuly 17, 2024Yuan Q, Fan Z, Huang W, Huo X, Yang X, Ran Y, Chen J, Li H.pGL3-Promoter-PD-L1, 0.2 µg of the pRL-TK Renilla plasmid (Promega, Madison, WI, USA; catalog number E2241), and specific expression plasmids using the Lipo293 transfection reagent (Beyotime, Shanghai, China;

(135) CREB3 suppresses hepatocellular carcinoma progression by depressing AKT signaling through competitively binding with insulin receptor and transcriptionally activating RNA‐binding motif protein 38MedComm (2020)July 1, 2024Yi He, Shenqi Han, Han Li, Yu Wu, Wenlong Jia, Zeyu Chen, Yonglong Pan, Ning Cai, Jingyuan Wen, Ganxun Li, et al.The sequences of siRNAs were listed in Table S7. For luciferase assay, pGL4.17 (E6721) and pRL‐TK (E2241) plasmids were purchased from Promega (WI, USA). Promoter sequence of RBM38 was synthesized (Tsingke

(136) Mutant TP53 promotes invasion of lung cancer cells by regulating desmoglein 3Journal of cancer research and clinical oncologyJune 20, 2024Feng Y, Qian R, Cui D, Luan J, Xu M, Wang L, Li R, Wu X, Chang C.pGL3 vector. The resulting pGL3 vector, along with the pRL-TK Renilla luciferase reporter plasmid (E2241, Promega, Madison, USA) as an internal control to standardize for transfection efficiency, was transfected

(137) N6-methyladenosine-driven miR-143/145-KLF4 circuit orchestrates the phenotypic switch of pulmonary artery smooth muscle cellsCellular and molecular life sciences : CMLSJune 12, 2024Kang K, Sun C, Li H, Liu X, Deng J, Chen S, Zeng L, Chen J, Liu X, Kuang J, et al.pGl4.15 (Promega, E6701) as a backbone, and a reference Renilla luciferase construct pRL-TK (Promega, E2241). A 1.6 kb putative promoter of miR-143/145 was constructed by PCR from rat genomic DNA. The A7r5 cells

(138) Transcription factor Nrf1 regulates proteotoxic stress-induced autophagyJournal of Cell BiologyJune 3, 2024Madison A. Ward, Janakiram R. Vangala, Hatem Elif Kamber Kaya, Holly A. Byers, Nayyerehalsadat Hosseini, Antonio Diaz, Ana Maria Cuervo, Susmita Kaushik, Senthil K. Radhakrishnanactivity, HEK293T cells were transfected with the promoter-luciferase constructs, pRL-TK (Cat. No. E2241; Promega) alone or in combination with Nrf1-p110 in a ratio of 3:1:6 using Lipofectamine 3000. The total

(139) Therapeutic targeting of telomerase ameliorates experimental choroidal neovascularizationBiochimica et Biophysica Acta (BBA) - Molecular Basis of DiseaseJune 1, 2024Aman Kumar, Yosuke Nagasaka, Vinodhini Jayananthan, Asmaa Zidan, Tyler Heisler-Taylor, Jayakrishna Ambati, Shigeo Tamiya, Nagaraj Keruror in combination. Renilla reporter plasmid (50ng) under HSV-thymidine kinase promoter (Promega Cat# E2241) was also transfected and was used for normalization. Cells were transfected at 50–60% confluence using

(140) Reduction of specific enterocytes from loss of intestinal LGR4 improves lipid metabolism in miceNature CommunicationsMay 23, 2024Yuan Liang, Chao Luo, Lijun Sun, Tiange Feng, Wenzhen Yin, Yunhua Zhang, Michael W. Mulholland, Weizhen Zhang, Yue Yin(83732996. 83733555)) was cloned to pGL3-basic vector. Cotransfected the plasmids together with pRL-TK (E2241, Promega, USA) into 293T cells. Harvest the cells after 24 h and conducted dual luciferase reporter

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pRL-TK Vector

pRL-TK Vector from Promega

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Promega

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pRL-TK Vector from Promega

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