pRL-TK Vector from Promega

Product Specs
ItempRL-TK Vector
CompanyPromega
Price $0.00Supplier Page View Company Product Page
Catalog NumberE2241
HostMammalian
PromoterHSV TK promoter
Vector NamepRL-TK
Quantity20microg
ReporterRenilla luciferase
ResistanceAmpR
Molecular Weight~73kDa
Selectable MarkerAmpicillin

Specifications/Features

Vector Size:
4045 bp

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(101) Cell-intrinsic regulation of HBV RNAs by the nonsense-mediated mRNA decay pathway controls viral replicationiScienceMay 16, 2025Masami Wada, Chiharu Morita, Eriko Ohsaki, Keiji Uedapaper,N/A pT7-2.1kb RNA,This paper,N/A pT7-Small S RNA,This paper,N/A pT7-XRNA,This paper,N/A pRL-TK,Promega,Cat#E2241 pcDNA-GFP,Nakayama et al.48,N/A pHBV-WT,Fauzyah et al.46 Sugiyama et al.47,N/A pHBV pol dead,This

(102) U2AF65 mediated circPVT1 promotes NSCLC cell proliferation and inhibits ferroptosis through the miR-338-3p/GPX4 axisCell Biol ToxicolMay 14, 2025Lujuan He, Zezhi Zhou, Jufen Wang, Jiehan Jiang, Shenggang LiuWI, USA) and 50 nM miR-338-3p mimic (HY-R00698, MCE, NJ, USA). Renilla luciferase plasmid pRL-TK (#E2241, Promega) served as an internal control. Transfection was performed utilizing Lipofectamine® 2000 (#11668019,

(103) Microtubule associated serine/threonine kinase-3 inhibits the malignant phenotype of breast cancer by promoting phosphorylation-mediated ubiquitination degradation of yes-associated proteinBreast Cancer ResMay 1, 2025Ning Deng, Wei Kang, Jiang Du, Xuezhu Rong, Qiang Hanluciferase plasmid (# 34615) was purchased from Addgene (Cambridge, MA, USA). The pRL-TK vector (# E2241) was purchased from Promega (Madison, Wisconsin, USA). shYAP (# sc-38637-SH) was purchased from Santa Cruz Biotechnology, Inc

(104) Polycyclic aromatic hydrocarbons promote tumorigenesis of gallbladder cancer via Aryl Hydrocarbon Receptor-HEGBC positive feedback axisiScienceApril 23, 2025Lei Yuan, Yumeng Zhang, Jianguo Xu, Feng Liu, Yingchao Liang, Beili Wang, Meihong Wu, Zhiyuan Fan, Liang Yangin their studies of AhR activation.39,40 A renilla luciferase construct control vector (pRLTK; Cat#E2241, Promega, Wisconsin, USA) was transfected into primary NHEKs with TransIT Keratinocyte transfection

(105) A blocking antibody against anti-Müllerian hormone restores ovulation and normal androgen levels in a spontaneous rat model of polycystic ovary syndromeEBioMedicineApril 18, 2025Chrystèle Racine, François Fraissinet, Stefania Tolu, Tony Pereira, Stéphanie Gil, Anne Badel, Danielle Bailbé, Bruno Fève, Jamileh Movassat, Richard Cate, et al.Gal4-Smad5 and Gal4-luc plasmids or Cyp19a1 or StAR reporter genes, and 12.5 ng/w of pRLTK vector (E2241, Promega, Madison, WI) as a control for transfection efficiency. For KK1 cells, the mouse AMHR2 cDNA

(106) A nuclear RNA degradation code is recognized by PAXT for eukaryotic transcriptome surveillanceMolecular CellApril 17, 2025Lindsey V. Soles, Liang Liu, Xudong Zou, Yoseop Yoon, Shuangyu Li, Lusong Tian, Marielle Valdez, Angela M Yu, Hong Yin, Wei Li, et al.pX330A-S-ZFC3H1-sgRNA,This study,N/A Plasmid: pGL3-promoter,Promega,Cat#E1751 Plasmid: pRL-TK,Promega,Cat#E2241 Plasmid: pCDNA5: FLAG-eGFP-5′ ss WT-bGH PAS,This study,N/A Plasmid: pCDNA5: FLAG-eGFP-5′ ss Mut-bGH

(107) High-Throughput Screening to Identify Novel Compounds Affecting the Genome Editing Efficiency of CRISPR SystemMoleculesApril 17, 2025Jiasong Chang, Xiulong Yang, Tong Zhang, Hao Sun, Hongying Cheng, Zhangrong Jia, Yiying Li, Sanyuan Ma, Teng Sun, Jimin Caothe SSA reporter vector, named T-CMV-SSA-luciferase. The Renilla luciferase expression vector pRL-TK (E2241) was purchased from Promega (Madison, WI, USA). The CRISPR vector pX330-U6-Chimeric_BB-CBh-hSpCas9 was

(108) Inhibition of Rho GEFs attenuates pulmonary fibrosis through suppressing myofibroblast activation and reprogramming profibrotic macrophagesCell Death DisApril 11, 2025Chengju Luo, Chenqi Huang, Yuqi Zhu, Yuxin Zhou, Yansheng Qiao, Chenxiao Shi, Yuan Gao, Yongjian Guo, Libin Weitransfected with 2 μg of 8xGTIIC-luciferase plasmid (Addgene, #34615) and 0.2 μg of pRL-TK plasmid (Promega, E2241) using jetPRIME transfection reagent (Polyplus, France). After drug treatment, Firefly luciferase and

(109) Mutant huntingtin induces neuronal apoptosis via derepressing the non-canonical poly(A) polymerase PAPD5Nat CommunApril 9, 2025Zhefan Stephen Chen, Shaohong Isaac Peng, Lok I Leong, Terence Gall-Duncan, Nathan Siu Jun Wong, Tsz Ho Li, Xiao Lin, Yuming Wei, Alex Chun Koon, Junzhe Huang, et al.-luciferase reporter assay (E1910, Promega) was carried out as described previously31. The pTK-RL Renilla luciferase vector (E2241, Promega) was used as an internal control reporter construct for the normalization of transfection efficiency.

(110) The long noncoding RNA APR attenuates PPRV infection-induced accumulation of intracellular iron to inhibit membrane lipid peroxidation and viral replicationmBioApril 9, 2025Bo Wen, Wenchi Chang, Lulu Yang, Daiyue Lv, Lizhen Wang, Lei Wang, Yanzhao Xu, Jianhe Hu, Ke Ding, Qinghong Xue, et al.(Promega, E665A). EECs were co-transfected with luciferase constructs and the pRLTK vector (Promega, E2241). The cells were harvested 36–48 h after transfection and were detected using the dual luciferase reporter assay

(111) Inhalable Hsa‐miR‐30a‐3p Liposomes Attenuate Pulmonary FibrosisAdvanced ScienceMarch 22, 2025Shuo Liu, Kristen D. Popowski, Christina M. Eckhardt, Weihang Zhang, Junlang Li, Yujia Jing, Dylan Silkstone, Elizabeth Belcher, Megan Cislo, Shiqi Hu, et al.pGL3‐Promoter‐miR‐30a, along with the miR‐30a mimic and the pRL‐TK renilla luciferase control reporter vectors (Promega, E2241), were co‐transfected into lung fibroblast cells. The Dual‐Luciferase Reporter Assay System (Promega,

(112) MAZ-mediated LAMA5 transcription activation promotes gastric cancer progression through the STAT3 signalingFunct Integr GenomicsMarch 12, 2025Yu Wang, Jiazhong Xu, Hongxia Zhang, Xiaobo Guo, Hongjun Liu, Qinhui Suntarget plasmids were subsequently co-transfected with pRL Renilla Luciferase Control Reporter Vectors (E2241, Promega Corporation, Madison, WI, USA) into sh-NC and sh-MAZ treated GC cells. After 48 h, the cells

(113) HHV-6B ribonucleotide reductase sequesters NF-κB subunit p65 to inhibit innate immune responsesiScienceFebruary 21, 2025Mansaku Hirai, Khoir Amaliin, Jing Rin Huang, Salma Aktar, Yasuko Mori, Jun Arii(NF-κB-luc),Promega,Cat # E8491 Plasmid: pRL-SV40,Promega,Cat # E2231 Plasmid: pRL-TK,Promega,Cat # E2241 Plasmid: pTK-N-LgBiT,Promega,Cat # N2014 Plasmid: pTK-C-LgBiT,Promega,Cat # N2014 Plasmid: pTK-N-SmBiT,Promega,Cat

(114) Superoxide dismutase promotes gastric tumorigenesis mediated by Helicobacter pylori and enhances resistance to 5-fluorouracil in gastric canceriScienceFebruary 21, 2025Hongbing Fu, Yu Zhang, Yantao Duan, Xin Zhang, Jun Yao, Dejun Yang, Ziran Wei, Zhenxin Zhu, Jiapeng Xu, Zunqi Hu, et al.DNA,Recombinant DNA,Recombinant DNA ,, pcDNA3.1 plasmid: SOD2,Invitrogen,N/A pRL-TK,Promega,Cat# E2241 pGL3-Basic vector,Promega,N/A ,, Software and algorithms,Software and algorithms,Software and algorithms ,, ImageJ

(115) Carboxyl Terminal Modulator Protein Induces Cell Senescence and Is Upregulated With Aging by Zic2 in RatsJ Cell PhysiolJanuary 30, 2025Weiran Shan, Jun Li, Zachary Philpot, Zhiyi Zuoplasmid,Genecopoeia,EX‐NEG‐M02 pGL3‐enhancer vector,Promega,E1771 Renilla luciferase vector pRL‐TK,Promega,E2241 Lipofectamine LTX,ThermoFisher Scientific,A12621 CTMP‐ORF,Genecopoeia,EX_Rn17554‐M56_GS M56‐Vector,Genecopoeia,EX‐NEG‐M56 EndoFectin Max transfection reagent

(116) Divergent Effects of Circoviridae Capsid Proteins on Type I Interferon SignalingPathogensJanuary 13, 2025Anon H. Kosaka, Chen-Yu Huang, Zih-Ying Lu, Hua-Zhen Hsing, Amonrat Choonnasard, Rissar Siringo Ringo, Kuo Pin Chuang, Akatsuki SaitoMyc-tagged human TRIF and pig TRIF, respectively, and the pRL–TK vector (Promega, Madison, WI, USA, Cat# E2241) were used to evaluate IFN-β signaling. The coding sequence of the Circoviridae capsid plasmids was

(117) The RING Finger E3 Ligase RNF25 Protects DNA Replication Forks Independently of its Canonical Roles in Ubiquitin SignalingbioRxivJanuary 9, 2025Lilly F. Chiou, Deepika Jayaprakash, Gaith N. Droby, Xingyuan Zhang, Yang Yang, C. Allie Mills, Thomas S. Webb, Natalie K. Barker, Di Wu, Laura E. Herring, et al.from Dr. Albert Baldwin at UNC Chapel Hill) and 1ng pRL (Renilla Luciferase Control Reporter, Promega E2241) for 24 h with media change after 6 h of transfection. Cell monolayers were further incubated in fresh

(118) SIX1 aggravates the progression of spinal cord injury in mice by promoting M1 polarization of microgliaScientific reportsJanuary 8, 2025Xu Z, Zhu M, Xie H, Zhu J, Zheng H, Liu X, Zhang Y, Liu J.promoter (pGL4-VEGF-C-WT), pGL4 plasmids with mutant VEGF-C promoter (pGL4-VEGF-C-MUT), or pRL-TK (E2241, Promega, USA), which was used as an internal control. Transfection was performed using Lipofectamine

(119) Pim3 up-regulation by YY1 contributes to diabetes-induced cardiac hypertrophy and heart failureIran J Basic Med SciJanuary 1, 2025Xiao-Ping Jin, Yi-Fei Ren, Li-Guo Wang, Hao Xie, Lu Huang, Juan Zhang, Zuo-Ying Huobtained from MiaoLing Plasmid Platform (Wuhan, Hubei, China). The pRL Renilla luciferase vector (pRL-TK, #E2241) was obtained from Promega (Madison, WI, US). HL-1 cells were plated at a density of 2×105 cells/well

(120) Phosphorylation of the selective autophagy receptor TAX1BP1 by TBK1 and IKBKE/IKKi promotes ATG8-family protein-dependent clearance of MAVS aggregatesAutophagyJanuary 1, 2025White J, Choi YB, Zhang J, Vo MT, He C, Shaikh K, Harhaj EW.with the desired plasmids together with IFNB-Luc [27] and the Renilla reporter pRL-TK-Luc (Promega, E2241) as an internal control. After 24 h, cells were lysed in passive lysis buffer (Promega, E1941) and subjected

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pRL-TK Vector

pRL-TK Vector from Promega

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Specifications/Features

Promega

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pRL-TK Vector from Promega

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