pRL-TK Vector from Promega

Product Specs
ItempRL-TK Vector
CompanyPromega
Price $0.00Supplier Page View Company Product Page
Catalog NumberE2241
HostMammalian
PromoterHSV TK promoter
Vector NamepRL-TK
Quantity20microg
ReporterRenilla luciferase
ResistanceAmpR
Molecular Weight~73kDa
Selectable MarkerAmpicillin

Specifications/Features

Vector Size:
4045 bp

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(101) Mutant huntingtin induces neuronal apoptosis via derepressing the non-canonical poly(A) polymerase PAPD5Nat CommunApril 9, 2025Zhefan Stephen Chen, Shaohong Isaac Peng, Lok I Leong, Terence Gall-Duncan, Nathan Siu Jun Wong, Tsz Ho Li, Xiao Lin, Yuming Wei, Alex Chun Koon, Junzhe Huang, et al.-luciferase reporter assay (E1910, Promega) was carried out as described previously31. The pTK-RL Renilla luciferase vector (E2241, Promega) was used as an internal control reporter construct for the normalization of transfection efficiency.

(102) The long noncoding RNA APR attenuates PPRV infection-induced accumulation of intracellular iron to inhibit membrane lipid peroxidation and viral replicationmBioApril 9, 2025Bo Wen, Wenchi Chang, Lulu Yang, Daiyue Lv, Lizhen Wang, Lei Wang, Yanzhao Xu, Jianhe Hu, Ke Ding, Qinghong Xue, et al.(Promega, E665A). EECs were co-transfected with luciferase constructs and the pRLTK vector (Promega, E2241). The cells were harvested 36–48 h after transfection and were detected using the dual luciferase reporter assay

(103) Inhalable Hsa‐miR‐30a‐3p Liposomes Attenuate Pulmonary FibrosisAdvanced ScienceMarch 22, 2025Shuo Liu, Kristen D. Popowski, Christina M. Eckhardt, Weihang Zhang, Junlang Li, Yujia Jing, Dylan Silkstone, Elizabeth Belcher, Megan Cislo, Shiqi Hu, et al.pGL3‐Promoter‐miR‐30a, along with the miR‐30a mimic and the pRL‐TK renilla luciferase control reporter vectors (Promega, E2241), were co‐transfected into lung fibroblast cells. The Dual‐Luciferase Reporter Assay System (Promega,

(104) MAZ-mediated LAMA5 transcription activation promotes gastric cancer progression through the STAT3 signalingFunct Integr GenomicsMarch 12, 2025Yu Wang, Jiazhong Xu, Hongxia Zhang, Xiaobo Guo, Hongjun Liu, Qinhui Suntarget plasmids were subsequently co-transfected with pRL Renilla Luciferase Control Reporter Vectors (E2241, Promega Corporation, Madison, WI, USA) into sh-NC and sh-MAZ treated GC cells. After 48 h, the cells

(105) HHV-6B ribonucleotide reductase sequesters NF-κB subunit p65 to inhibit innate immune responsesiScienceFebruary 21, 2025Mansaku Hirai, Khoir Amaliin, Jing Rin Huang, Salma Aktar, Yasuko Mori, Jun Arii(NF-κB-luc),Promega,Cat # E8491 Plasmid: pRL-SV40,Promega,Cat # E2231 Plasmid: pRL-TK,Promega,Cat # E2241 Plasmid: pTK-N-LgBiT,Promega,Cat # N2014 Plasmid: pTK-C-LgBiT,Promega,Cat # N2014 Plasmid: pTK-N-SmBiT,Promega,Cat

(106) Superoxide dismutase promotes gastric tumorigenesis mediated by Helicobacter pylori and enhances resistance to 5-fluorouracil in gastric canceriScienceFebruary 21, 2025Hongbing Fu, Yu Zhang, Yantao Duan, Xin Zhang, Jun Yao, Dejun Yang, Ziran Wei, Zhenxin Zhu, Jiapeng Xu, Zunqi Hu, et al.DNA,Recombinant DNA,Recombinant DNA ,, pcDNA3.1 plasmid: SOD2,Invitrogen,N/A pRL-TK,Promega,Cat# E2241 pGL3-Basic vector,Promega,N/A ,, Software and algorithms,Software and algorithms,Software and algorithms ,, ImageJ

(107) Carboxyl Terminal Modulator Protein Induces Cell Senescence and Is Upregulated With Aging by Zic2 in RatsJ Cell PhysiolJanuary 30, 2025Weiran Shan, Jun Li, Zachary Philpot, Zhiyi Zuoplasmid,Genecopoeia,EX‐NEG‐M02 pGL3‐enhancer vector,Promega,E1771 Renilla luciferase vector pRL‐TK,Promega,E2241 Lipofectamine LTX,ThermoFisher Scientific,A12621 CTMP‐ORF,Genecopoeia,EX_Rn17554‐M56_GS M56‐Vector,Genecopoeia,EX‐NEG‐M56 EndoFectin Max transfection reagent

(108) Divergent Effects of Circoviridae Capsid Proteins on Type I Interferon SignalingPathogensJanuary 13, 2025Anon H. Kosaka, Chen-Yu Huang, Zih-Ying Lu, Hua-Zhen Hsing, Amonrat Choonnasard, Rissar Siringo Ringo, Kuo Pin Chuang, Akatsuki SaitoMyc-tagged human TRIF and pig TRIF, respectively, and the pRL–TK vector (Promega, Madison, WI, USA, Cat# E2241) were used to evaluate IFN-β signaling. The coding sequence of the Circoviridae capsid plasmids was

(109) The RING Finger E3 Ligase RNF25 Protects DNA Replication Forks Independently of its Canonical Roles in Ubiquitin SignalingbioRxivJanuary 9, 2025Lilly F. Chiou, Deepika Jayaprakash, Gaith N. Droby, Xingyuan Zhang, Yang Yang, C. Allie Mills, Thomas S. Webb, Natalie K. Barker, Di Wu, Laura E. Herring, et al.from Dr. Albert Baldwin at UNC Chapel Hill) and 1ng pRL (Renilla Luciferase Control Reporter, Promega E2241) for 24 h with media change after 6 h of transfection. Cell monolayers were further incubated in fresh

(110) SIX1 aggravates the progression of spinal cord injury in mice by promoting M1 polarization of microgliaScientific reportsJanuary 8, 2025Xu Z, Zhu M, Xie H, Zhu J, Zheng H, Liu X, Zhang Y, Liu J.promoter (pGL4-VEGF-C-WT), pGL4 plasmids with mutant VEGF-C promoter (pGL4-VEGF-C-MUT), or pRL-TK (E2241, Promega, USA), which was used as an internal control. Transfection was performed using Lipofectamine

(111) Pim3 up-regulation by YY1 contributes to diabetes-induced cardiac hypertrophy and heart failureIran J Basic Med SciJanuary 1, 2025Xiao-Ping Jin, Yi-Fei Ren, Li-Guo Wang, Hao Xie, Lu Huang, Juan Zhang, Zuo-Ying Huobtained from MiaoLing Plasmid Platform (Wuhan, Hubei, China). The pRL Renilla luciferase vector (pRL-TK, #E2241) was obtained from Promega (Madison, WI, US). HL-1 cells were plated at a density of 2×105 cells/well

(112) Phosphorylation of the selective autophagy receptor TAX1BP1 by TBK1 and IKBKE/IKKi promotes ATG8-family protein-dependent clearance of MAVS aggregatesAutophagyJanuary 1, 2025White J, Choi YB, Zhang J, Vo MT, He C, Shaikh K, Harhaj EW.with the desired plasmids together with IFNB-Luc [27] and the Renilla reporter pRL-TK-Luc (Promega, E2241) as an internal control. After 24 h, cells were lysed in passive lysis buffer (Promega, E1941) and subjected

(113) Oklahoma nitrone-007 is an effective anticancer therapeutic agent targeting inflammatory and immune metabolism pathways in endometrial cancerThe Journal of Pharmacology and Experimental TherapeuticsJanuary 1, 2025Sugantha Priya Elayapillai, Anjalika Gandhi, Samrita Dogra, Debra Saunders, Nataliya Smith, Cole Hladik, Rheal A. Towner, Katherine M. Moxley, Bethany N. Hannafon(SBE4-Luc, pNF-κB-Luc, or XRE-Luc) along with 20 ng Renilla luciferase reporter pRL-TK plasmid DNA (E2241; Promega) using the X-tremeGENE 360 transfection reagent (XTG360-RO; Sigma) in a six-well plate according

(114) Long non-coding RNA Malat1 fine-tunes bone homeostasis and repair by orchestrating cellular crosstalk and β-catenin-OPG/Jagged1 pathwayElifeDecember 23, 2024Yongli Qin, Jumpei Shirakawa, Cheng Xu, Ruge Chen, Xu Yang, Courtney Ng, Shinichi Nakano, Mahmoud Elguindy, Zhonghao Deng, Kannanganattu V Prasanth, et al.plasmid of M50 Super 8 x TOPFlash (Cat# 12456) was purchased from Addgene. pRL-Tk control plasmid (Cat# E2241) was purchased from Promega. Fetal Bovine Serum (FBS, Cat #S11550) was obtained from Atlanta Biologicals.

(115) Protocol to study cis-regulatory activity of GWAS loci for specific gene promoters in human primary astrocytes using luciferase reporter assaySTAR ProtocolsDecember 20, 2024Sheridan H. Littleton, Struan F.A. GrantDNA,Recombinant DNA ,, pGL4.10[luc2] reporter vector,Promega,Cat# E6651 pRL-TK reporter vector,Promega,Cat# E2241 FAIM2 promoter clone in pEZX-PG02 reporter vector,GeneCopoeia,Cat# HPRM47354-PG02 pGL4.10[luc2]-rs7132908G-FAIM2,Littleton

(116) Single-nucleus multi-omics analyses reveal cellular and molecular innovations in the anterior cingulate cortex during primate evolutionCell GenomicsDecember 11, 2024Jiamiao Yuan, Kangning Dong, Haixu Wu, Xuerui Zeng, Xingyan Liu, Yan Liu, Jiapei Dai, Jichao Yin, Yongjie Chen, Yongbo Guo, et al.(Promega, E1751). 500 ng pGL3-SNC/HAR-promoter/basic or pGL3-promoter/basic and 25ng pRL-TK (Promega, E2241) were transfected into corresponding cells in 24-well plates using lipofectamine 3000 (Invitrogen, L3000015).

(117) Aryl Hydrocarbon Receptor Alleviates Hepatic Fibrosis by Inducing Hepatic Stellate Cell FerroptosisJournal of cellular and molecular medicineDecember 1, 2024Liu S.site‐directed mutagenesis on the recombinant plasmid. The above plasmids together with the phRL‐TK (Promega, E2241) were co‐transfected into Hep1‐6 cells, using Lipofectamine 2000 (Invitrogen, 11668019). After 6 h incubation,

(118) PIN1 is a novel interaction partner and a negative upstream regulator of the transcription factor NFIBFEBS LettersDecember 1, 2024Sinem Saritas Erdogan, Ahmet Erdal Yilmaz, Asli Kumbasaras expression vectors encoding HA‐NFIB and/or FLAG‐PIN1 constructs. pRL‐TK renilla reporter plasmid (E2241; Promega) was included as normalization control. Forty‐eight hours after the transfection, cells were

(119) FOSL1 is a key regulator of a super-enhancer driving TCOF1 expression in triple-negative breast cancerEpigenetics & ChromatinNovember 10, 2024Qingling He, Jianyang Hu, Hao Huang, Tan Wu, Wenxiu Li, Saravanan Ramakrishnan, Yilin Pan, Kui Ming Chan, Liang Zhang, Mengsu Yang, et al.(Promega, #E1761). Plasmid pGL3-Promoter-TCOF1 E1 was co-transfected with pRL-TK vector (Promega, #E2241) into HCC1806 or MCF10-DCIS cells using FuGENE 6 transfection reagent (Promega, #2693). A pRL-TK vector

(120) Inhibition of the Notch signal transducer CSL by Pkc53E-mediated phosphorylation to fend off parasitic immune challenge in DrosophilaeLifeNovember 6, 2024Deichsel S, Frankenreiter L, Fechner J, Gahr BM, Zimmermann M, Mastel H, Preis I, Preiss A, Nagel AC.1994",N/A, Recombinant DNA reagent,pCDNA3.1,Invitrogen,Cat# V79020, Recombinant DNA reagent,pRL TK,Promega,Cat# E2241, "Strain, strain background (Leptopilina boulardi)",Leptopilina boulardi,"Häußling, J Stökl, Bayreuth",N/A,Parasitoid

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pRL-TK Vector

pRL-TK Vector from Promega

Description

Specifications/Features

Promega

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Reviews

pRL-TK Vector from Promega

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