pRL-TK Vector from Promega

Product Specs
ItempRL-TK Vector
CompanyPromega
Price $0.00Supplier Page View Company Product Page
Catalog NumberE2241
HostMammalian
PromoterHSV TK promoter
Vector NamepRL-TK
Quantity20microg
ReporterRenilla luciferase
Molecular Weight~73kDa
Selectable MarkerAmpicillin
ResistanceAmpR

Specifications/Features

Vector Size:
4045 bp

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(181) A microRNA Arising from the Negative Strand of SARS-CoV-2 Genome Targets FOS to Reduce AP-1 ActivityNon-Coding RNAMay 23, 2023Francesco Greco, Elisa Lorefice, Claudia Carissimi, Ilaria Laudadio, Fabiola Ciccosanti, Martina Di Rienzo, Francesca Colavita, Silvia Meschi, Fabrizio Maggi, Gian Maria Fimia, et al.(HCT116) or 100 ng (HEK293T) 3xAP1pGL3 reporter and 25 ng (HCT116) or 10 ng (HEK293T) pRL-TK (Promega E2241) plasmid were transfected using Jetprime transfection reagent (Polyplus 115). Eighteen hours later renilla

(182) Ctnnb1/β-catenin inactivation in UCP1-positive adipocytes augments the browning of white adipose tissueiScienceMay 19, 2023Chen N, Yuan M, Zhang N, Chen M, Liu R, Wang J, Lu P.pGL4.12,Promega,Cat#E6671 Plasmid: pGL4.12-UCP1 promoter,This paper,N/A Plasmid: pRL-TK,Promega,Cat#E2241 ,, Software and algorithms,Software and algorithms,Software and algorithms ,, GraphPad Prism (v9.0.0),GraphPad,http://www.graphpad.com/scientific-software/prism/ SPSS

(183) miR‑4732‑5p promotes ovarian cancer mobility by targeting MCUR1Oncology LettersApril 20, 2023Xin Li, Xiaoqin Wang, Jun Wu“CUCUACA” and corresponding mutation with sequences of “AGAGCAG”, 26 ng of pRL-TK plasmids (cat. no. E2241, Promega Corporation) containing Renilla luciferase, along with 10 pmol/ml miR-4732-5p mimics or negative

(184) Genetic regulatory and biological implications of the 10q24.32 schizophrenia risk locusBrainApril 19, 2023Junyang Wang, Jiewei Liu, Shiwu Li, Xiaoyan Li, Jinfeng Yang, Xinglun Dang, Changgai Mu, Yifan Li, Kaiqin Li, Jiao Li, et al.respectively. The recombinant vectors (150 ng) and the pRL-TK (internal control, 50 ng, Promega, Cat. No. E2241) were then co-transfected into three cell lines (HEK293T, SK-N-SH and SH-SY5Y) using the LipofectamineTM

(185) Single Nucleotide Polymorphism rs9277336 Controls the Nuclear Alpha Actinin 4‐Human Leukocyte Antigen‐DPA1 Axis and Pulmonary Endothelial Pathophenotypes in Pulmonary Arterial HypertensionJournal of the American Heart AssociationApril 4, 2023Neha Hafeez, Anna Kirillova, Yunshan Yue, Rashmi J. Rao, Neil J. Kelly, Wadih El Khoury, Yassmin Al Aaraj, Yi‐Yin Tai, Adam Handen, Ying Tang, et al.amount of the vector pRL‐TK that provides constitutive expression of Renilla luciferase (Catalog #: E2241, Promega). The firefly luciferase reporter activity was measured by normalizing the firefly luciferase

(186) Single Nucleotide Polymorphism rs9277336 Controls the Nuclear Alpha Actinin 4‐Human Leukocyte Antigen‐DPA1 Axis and Pulmonary Endothelial Pathophenotypes in Pulmonary Arterial HypertensionJ Am Heart AssocMarch 28, 2023Neha Hafeez, Anna Kirillova, Yunshan Yue, Rashmi J. Rao, Neil J. Kelly, Wadih El Khoury, Yassmin Al Aaraj, Yi‐Yin Tai, Adam Handen, Ying Tang, et al.amount of the vector pRL‐TK that provides constitutive expression of Renilla luciferase (Catalog #: E2241, Promega). The firefly luciferase reporter activity was measured by normalizing the firefly luciferase

(187) PHF6 recruits BPTF to promote HIF-dependent pathway and progression in YAP-high breast cancerJournal of Translational MedicineMarch 26, 2023Sheng Gao, Wensheng Zhang, Jingjing Ma, Xiaojian Niwith 200 ng of YAP/YAP-mutant construct and 10 ng of Renilla luciferase pRL-TK plasmid (Promega, Cat.#E2241). After 48 h, the dual luciferase assay was performed using the Dual-Luciferase® Reporter Assay System

(188) Identification of Potentially Functional Circular RNA/Long Noncoding RNA-MicroRNA-mRNA Regulatory Networks Associated with Vascular Injury in Type 2 Diabetes Mellitus by Integrated Microarray AnalysisJournal of Diabetes ResearchMarch 10, 2023Yi Leng, Ming-zhu Wang, Kang-ling Xie, Ying Caibioinformatically predicted miR-92a-2-5p to target genes. With pRL-TK vector expressing Renilla luciferase (E2241, Promega) as an internal reference, miR-92a-2-5p mimic and negative control were cotransfected with

(189) Long Non-Coding RNA TUG1 Attenuates Insulin Resistance in Mice with Gestational Diabetes Mellitus via Regulation of the MicroRNA-328-3p/SREBP-2/ERK AxisDiabetes & metabolism journalMarch 1, 2023Tang X, Qin Q, Xu W, Zhang X.site between miR-328-3p and SREBP-2, pSREBP2-mutant (MUT) vectors were constructed. The pRL-TK vector (E2241, Promega) carrying renilla luciferase was adopted as an internal control. miR-328-3p mimic or NC mimic

(190) Alcam-a and Pdgfr-α are essential for the development of sclerotome-derived stromal cells that support hematopoiesisNature CommunicationsMarch 1, 2023Emi Murayama, Catherine Vivier, Anne Schmidt, Philippe Herbomelluciferase vectors (pGL-alcama-luc or pGL-pdgfra-luc), control (Renilla) expression vector (pRL-TK; E2241, Promega), and a specified amount of Morpholino or appropriate mRNA encoding TF (co-injection condition

(191) Krüppel-like factor 9 (KLF9) links hormone dysregulation and circadian disruption to breast cancer pathogenesisCancer Cell InternationalFebruary 23, 2023Weand S. Ybañez, Pia D. BagamasbadpGL4.23-eKSM/aKSM/KDE or the positive control pGL4.23-ERRFI1 downstream enhancer (EDE) [33], and 25 ng pRL-TK (Promega, E2241) as a normalization control using the XtremeGENE HP DNA Transfection Reagent (Roche, 6366236001) as

(192) T-REX17 is a transiently expressed non-coding RNA essential for human endoderm formationeLifeJanuary 31, 2023Landshammer A, Bolondi A, Kretzmer H, Much C, Buschow R, Rose A, Wu HJ, Mackowiak SD, Braendl B, Giesselmann P, et al.with final 10 µM Y-27632. Sixteen hours later, cells were co-transfected with 15 fmol pRL-TK (Promega, E2241) and 150 fmol of either pGL4.27[luc2P/minP/Hygro] empty vector or pGL4.27[luc2P/minP/Hygro] containing

(193) Molecular differences of angiogenic versus vessel co-opting colorectal cancer liver metastases at single-cell resolutionMolecular cancerJanuary 24, 2023Fleischer JR, Schmitt AM, Haas G, Xu X, Zeisberg EM, Bohnenberger H, Küffer S, Teuwen LA, Karras PJ, Beißbarth T, et al.or mutated Tcf/Lef binding sites (Addgene, 12,456; Addgene, 12,457) and Renilla plasmids (Promega, E2241) were co-transfected in H1299 cells using Lipofectamine3000 (Invitrogen, USA; 15,282,465). Transfected

(194) Neurog2 regulates Isl1 to modulate horizontal cell numberDevelopmentJanuary 1, 2023Patrick W. Keeley, Pooja S. Patel, Matthew S. Ryu, Benjamin E. Reesethat every well received the same total molar equivalent of plasmids; additionally, a pRL-TK plasmid (E2241, Promega), in which the TK promoter drives the expression of Renilla luciferase, was added to every

(195) A rapid RIG-I signaling relay mediates efficient antiviral responseMolecular CellJanuary 1, 2023Daniel T. Thoresen, Drew Galls, Benjamin Götte, Wenshuai Wang, Anna M. Pylepaper,N/A pUNO1-IRF3 and fluorescent-tagged variants,"Invivogen, this paper",pUNO1-hIRF3 pRL-TK plasmid,Promega,E2241 IFN-β/Firefly luciferase plasmid,Ren et al.40,N/A mNG-N1,Allele Biotech,ABP-FP-MNEONSB Software and

(196) A Gypsy element contributes to the nuclear retention and transcriptional regulation of the resident lncRNA in locustsRNA BiologyDecember 31, 2022Xia Zhang, Ya′Nan Zhu, Bing Chen, Le Kangnegative control vector into cells with 5 ng of the internal control vector pRL-TK (Promega, WI, USA; E2241) to express Renilla luciferase. Both firefly and Renilla luciferase activities were measured using a

(197) The Highly Potent AhR Agonist Picoberin Modulates Hh-Dependent Osteoblast DifferentiationJournal of medicinal chemistryDecember 22, 2022Flegel J, Shaaban S, Jia ZJ, Schulte B, Lian Y, Krzyzanowski A, Metz M, Schneidewind T, Wesseler F, Flegel A, et al.XRE-dependent Firefly luciferase (Promega, #9PIE412) and constitutively expressed Renilla luciferase (Promega, #E2241) for 24 h. Afterward, 50,000 cells were seeded per well into 96-well plates (Corning, #353075) and incubated

(198) Dynamic 3D genome reorganization during development and metabolic stress of the porcine liverCell DiscoveryDecember 1, 2022Luxi Chen et al.Opti-MEM (Gibco, 11058021), with three technical replicates for each construct. And TK-15 (Promega, E2241) was cotransfected for each as a normalization control. Fluorescence values were determined using a

(199) ZNF498 promotes hepatocellular carcinogenesis by suppressing p53-mediated apoptosis and ferroptosis via the attenuation of p53 Ser46 phosphorylationJournal of Experimental & Clinical Cancer ResearchDecember 1, 2022Xiuyuan Zhang et al.transfected into HepG2 cells with other expression plasmids; the pRL-TK Renilla luciferase reporter plasmid (E2241, Promega, Madison, USA) served as an internal control to normalize for transfection efficiency. After

(200) A marine sponge-derived lectin reveals hidden pathway for thrombopoietin receptor activationNature CommunicationsNovember 25, 2022Hiromi Watari, Hiromu Kageyama, Nami Masubuchi, Hiroya Nakajima, Kako Onodera, Pamela J. Focia, Takumi Oshiro, Takashi Matsui, Yoshio Kodera, Tomohisa Ogawa, et al.described previously21. Briefly, pcDNA3.1 with MPL cDNA, pGL4.52 (Promega #E4651), and pRL–TK (Promega #E2241) were co-transfected into HEK293T cells using lipofectamine 2000 (Invitrogen #11668019). MPL agonists

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pRL-TK Vector

pRL-TK Vector from Promega

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Promega

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pRL-TK Vector from Promega

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