pRL-TK Vector from Promega

Product Specs
ItempRL-TK Vector
CompanyPromega
Price $0.00Supplier Page View Company Product Page
Catalog NumberE2241
HostMammalian
PromoterHSV TK promoter
Vector NamepRL-TK
Quantity20microg
ReporterRenilla luciferase
ResistanceAmpR
Molecular Weight~73kDa
Selectable MarkerAmpicillin

Specifications/Features

Vector Size:
4045 bp

Add to Compare List

Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(141) Conserved Functions of Orthohepadnavirus X Proteins to Inhibit Type-I Interferon SignalingInternational Journal of Molecular SciencesMarch 28, 2024Amonrat Choonnasard, Maya Shofa, Tamaki Okabayashi, Akatsuki Saitohttp://n2t.net/addgene:32713; accessed on 21 February 2024; RRID:Addgene_32713) [56]. pRL-TK (Promega, Madison, WI, USA, Cat# E2241) and pGL4.45[luc2P/ISRE/Hygro] (Promega, Cat# E4141) are commercially available. cDNA sequences of 17

(142) Integrative high-throughput enhancer surveying and functional verification divulges a YY2-condensed regulatory axis conferring risk for osteoporosisCell GenomicsMarch 13, 2024Xiao-Feng Chen, Yuan-Yuan Duan, Ying-Ying Jia, Qian-Hua Dong, Wei Shi, Yan Zhang, Shan-Shan Dong, Meng Li, Zhongbo Liu, Fei Chen, et al.cells,Transgene,CD501 hSTARR-seq_ORI vector,Addgene,99296 pGL3-basic vector,Promega,E1751 pRL-TK Renilla vector,Promega,E2241 ,, Biological samples,Biological samples,Biological samples ,, Human MSCs,"Shaanxi Provincial People’s

(143) Combined Effects of Polystyrene Nanosphere and Homosolate Exposures on Estrogenic End Points in MCF-7 Cells and ZebrafishEnviron Health PerspectFebruary 21, 2024Rongyi Ye, Zhiming Li, Hongyi Xian, Yizhou Zhong, Boxuan Liang, Yuji Huang, Da Chen, Mingzhu Dai, Shuqin Tang, Jie Guo, et al.in the Supplemental Material). In addition, the TK promoter-Renilla luciferase construct (pRL-TK, #E2241) was purchased from Promega. HEK-293T cells are frequently employed in the dual luciferase assay.55

(144) Combined Effects of Polystyrene Nanosphere and Homosolate Exposures on Estrogenic End Points in MCF-7 Cells and ZebrafishEnvironmental Health PerspectivesFebruary 21, 2024Rongyi Ye, Zhiming Li, Hongyi Xian, Yizhou Zhong, Boxuan Liang, Yuji Huang, Da Chen, Mingzhu Dai, Shuqin Tang, Jie Guo, et al.in the Supplemental Material). In addition, the TK promoter-Renilla luciferase construct (pRL-TK, #E2241) was purchased from Promega. HEK-293T cells are frequently employed in the dual luciferase assay.55

(145) Ebola virus sequesters IRF3 in viral inclusion bodies to evade host antiviral immunityElifeJanuary 29, 2024Lin Zhu, Jing Jin, Tingting Wang, Yong Hu, Hainan Liu, Ting Gao, Qincai Dong, Yanwen Jin, Ping Li, Zijing Liu, et al.p4cis-vRNA-RLuc (plasmid),DOI: 10.3791/52381,, Recombinant DNA reagent,pRL-TK vector (plasmid),Promega,Cat# E2241, Recombinant DNA reagent,pGL3-IFNβ-Luc (plasmid),This paper,,pGL3-basic vector Recombinant DNA reagent,pGL3-Promoter

(146) Ebola virus sequesters IRF3 in viral inclusion bodies to evade host antiviral immunityeLifeJanuary 29, 2024Lin Zhu, Jing Jin, Tingting Wang, Yong Hu, Hainan Liu, Ting Gao, Qincai Dong, Yanwen Jin, Ping Li, Zijing Liu, et al.p4cis-vRNA-RLuc (plasmid),DOI: 10.3791/52381,, Recombinant DNA reagent,pRL-TK vector (plasmid),Promega,Cat# E2241, Recombinant DNA reagent,pGL3-IFNβ-Luc (plasmid),This paper,,pGL3-basic vector Recombinant DNA reagent,pGL3-Promoter

(147) c-Fos regulated by TMPO/ERK axis promotes 5-FU resistance via inducing NANOG transcription in colon cancerCell death & diseaseJanuary 17, 2024Gui Y, Qian X, Ding Y, Chen Q, Fangyu Ye, Ye Y, Hou Y, Yu J, Zhao L.HEK-293T cells transfected with the Firefly luciferase and Renilla luciferase plasmids (pRL-TK, Promega, #E2241). Immunohistochemistry Immunohistochemistry assay was performed according to the previous method [18]. Xenograft

(148) Functional Analysis of KAP1/TRIM28 Requirements for HIV-1 Transcription ActivationVirusesJanuary 13, 2024Keyera Randolph, Usman Hyder, Ashwini Challa, Erick Perez, Iván D’OrsopcDNA3.1-HIV-1–LTR-FFL LUC reporter vector (100 ng/well) [41], pRL-TK (Promega, Madison, WI, USA, catalog E2241) (25 ng/well), and carrier DNA (pBluescript II KS+) to complete 250 ng total DNA per well. Five h post

(149) Tumor-immune microenvironment and NRF2 associate with clinical efficacy of PD-1 blockade combined with chemotherapy in lung squamous cell carcinomaCell Reports MedicineDecember 19, 2023Jianchun Duan, Yun Zhang, Ran Chen, Liang Liang, Yi Huo, Shun Lu, Jun Zhao, Chunhong Hu, Yuping Sun, Kunyu Yang, et al.paper,N/A pLEX-NRF2-E79K-CMV-puro,This paper,N/A pGL3-WT,This paper,N/A pGL3-Mutant,This paper,N/A pRL-TK Vector,Promega,Cat# E2241 ,, Software and algorithms,Software and algorithms,Software and algorithms ,, R package ‘GSVA’,Hänzelmann

(150) Targeting ST8SIA6-AS1 counteracts KRASG12C inhibitor resistance through abolishing the reciprocal activation of PLK1/c-Myc signalingExp Hematol OncolDecember 16, 2023Yafang Wang, Mingyue Yao, Cheng Li, Kexin Yang, Xiaolong Qin, Lansong Xu, Shangxuan Shi, Chengcheng Yu, Xiangjun Meng, Chengying Xieconstructs were co-transfected with 250 ng of Renilla luciferase-expressing control vector pRL-TK (E2241, Promega) into 293T or MIA PaCa-2 cells for 24 h. Cells were lysed with 1× passive lysis buffer (E1941,

(151) The glucocorticoid dexamethasone inhibits HIF-1α stabilization and metabolic reprogramming in lipopolysaccharide-stimulated primary macrophagesDiscovery ImmunologyDecember 4, 2023Sally A Clayton, Chloe Lockwood, John D O’Neil, Kalbinder K Daley, Sofia Hain, Dina Abdelmottaleb, Oliwia O Bolimowska, Daniel A Tennant, Andrew R ClarkAfter 24 h, in OptiMEM medium (#31985062, Gibco), cells were co-transfected with 10 µg TK-Renilla (#E2241, Promega) and 10 µg HRE-luciferase (a gift from Navdeep Chandel, this plasmid contains three copies

(152) Functional analysis of recurrent CDC20 promoter variants in human melanomaCommunications BiologyNovember 29, 2023Paula M. Godoy, Abimbola Oyedeji, Jacqueline L. Mudd, Vasilios A. Morikis, Anna P. Zarov, Gregory D. Longmore, Ryan C. Fields, Charles K. KaufmanSequences that were successfully mutated, as well as the WT pGL3-Basic vector and pRL-TK (Promega, E2241), were midi-prepped (Qiagen, 12941). For all cell lines, 300,000 cells per well were seeded onto 6-well

(153) Differential regulation of MYC expression by PKHD1/Pkhd1 in human and mouse kidneys: phenotypic implications for recessive polycystic kidney diseaseFrontiers in Cell and Developmental BiologyNovember 17, 2023Naoe Harafuji, Chaozhe Yang, Maoqing Wu, Girija Thiruvengadam, Heather Gordish-Dressman, R. Griffin Thompson, P. Darwin Bell, Avi Z. Rosenberg, Claudia Dafinger, Max C. Liebau, et al.pGL4.22 [luc2CP/Puro] vector was purchased from Promega (Cat. No. E6771). pRL-TK vector was purchased from promega (Cat. No. E2241). pGL4.22-mouse Myc P1 (pGL4.22-mMyc P1): the mouse Myc P1 promoter (chr8:127735983-127736125, GRCm38/mm10

(154) The fusion gene hsf5-rnf43 in Nile tilapia: A potential regulator in the maintenance of testis function and sexual differentiationiScienceNovember 17, 2023Yawei Shen, Hewei Jiang, Adelino V.M. Canario, Tiantian Chen, Yufei Liu, Guokun Yang, Xiaolin Meng, Jinliang Zhao, Xiaowu ChenDNA,Recombinant DNA ,, Plasmid: pGL3-basic,Promega,Cat. No. E1751 Plasmid: pRL-TK,Promega,Cat. No. E2241 ,, Software and algorithms,Software and algorithms,Software and algorithms ,, Expasy,Duvaud et al.35,http://www.au.expasy.org/ BLAST

(155) 4-(3-Phenyl-4-(3,4,5-trimethoxybenzoyl)-1H-pyrrol-1-yl)benzenesulfonamide, a Novel Carbonic Anhydrase and Wnt/β-Catenin Signaling Pathway Dual-Targeting Inhibitor with Potent Activity against Multidrug Resistant Cancer CellsJournal of medicinal chemistryNovember 9, 2023Masci D, Puxeddu M, Di Magno L, D'Ambrosio M, Parisi A, Nalli M, Bai R, Coluccia A, Sciò P, Orlando V, et al.FOPFlash Addgene #12457) was transfected in each well in combination with 10 ng of TK Renilla (Promega #E2241) and empty vector (pcDNA3.1) up to 100 ng. After 24 h, cells were incubated with starvation media (Opti-MEM

(156) Controlling Trophoblast Cell Fusion in the Human Placenta—Transcriptional Regulation of Suppressyn, an Endogenous Inhibitor of Syncytin-1BiomoleculesNovember 7, 2023Jun Sugimoto, Danny J. Schust, Makiko Sugimoto, Yoshihiro Jinno, Yoshiki Kudoco-transfected with 200 ng of pGL3 firefly luciferase plasmid and 10 ng of pRL-TK renilla luciferase plasmid (E2241; Promega, Madison, WI, USA) using Lipofectamine 2000 (11668027; Life Technologies, Carlsbad, CA, USA)

(157) miR-135a-5p overexpression in peripheral blood-derived exosomes mediates vascular injury in type 2 diabetes patientsFront Endocrinol (Lausanne)November 3, 2023Kangling Xie, Cui Li, Mingzhu Wang, Siqian Fu, Ying Caitarget gene, and the resulting vector was named pATM-Mut. The pRL-TK vector (Promega Co., Ltd., USA, E2241) expressing Renilla luciferase was used as an internal reference. HEK293 cells (ATCC, USA, CRL-11268)

(158) Direct and biologically significant interactions of human herpesvirus 8 interferon regulatory factor 1 with STAT3 and Janus kinase TYK2PLoS pathogensNovember 1, 2023Yang Z, Xiang Q, Nicholas J.(Addgene, #8688), pRL-TK, expressing Renilla luciferase constitutively, was obtained from Promega (#E2241), and pSBE4-Luc was provided by Dr. B Vogelstein (Addgene, #16495). Lentivirus production Lentivirus

(159) Preferential Regulation of Γ-Secretase-Mediated Cleavage of APP by Ganglioside GM1 Reveals a Potential Therapeutic Target for Alzheimer's DiseaseAdvanced science (Weinheim, Baden-Wurttemberg, Germany)November 1, 2023Wang X, Zhou R, Sun X, Li J, Wang J, Yue W, Wang L, Liu H, Shi Y, Zhang D.C99‐ Gal4 plasmid, 0.32 µg pGL4.35 plasmid (E1370;Promega , Madison, USA) and 0.008 µg pRL‐TK plasmid (E2241;Promega, Madison, USA) with Lipofectamine 2000 reagent (Invitrogen, CA, USA) according to manufacturer's

(160) 15-keto-PGE(2) alleviates nonalcoholic steatohepatitis through its covalent modification of NF-κB factorsiScienceOctober 20, 2023Hee SW, Chang YC, Su L, Chen IJ, Jeng YM, Hsieh ML, Chang YC, Li FA, Liao D, Chen SM, et al.Li-Chung Hsu, National Taiwan University",N/A Renilla luciferase reporter plasmid (pRL-TK),"Promega, WI",E2241 NF-κB1 (p105/p50),"Origene, MD",MR226792 RelA (p65),"Origene, MD",MR227671 ,, Software and algorithms,Software

Be the First to Write a Review!

pRL-TK Vector

pRL-TK Vector from Promega

Description

Specifications/Features

Promega

Citations (553)

Reviews

pRL-TK Vector from Promega

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map