pRL-TK Vector from Promega

Product Specs
ItempRL-TK Vector
CompanyPromega
Price $0.00Supplier Page View Company Product Page
Catalog NumberE2241
HostMammalian
PromoterHSV TK promoter
Vector NamepRL-TK
Quantity20microg
ReporterRenilla luciferase
ResistanceAmpR
Molecular Weight~73kDa
Selectable MarkerAmpicillin

Specifications/Features

Vector Size:
4045 bp

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(81) Rosuvastatin Upregulates RCOR1 to Repress C10ORF10 Transcription and Alleviate Oxidative Stress and Plaque Formation in AtherosclerosisThe Kaohsiung Journal of Medical SciencesSeptember 9, 2025Xin Fu, Chuang Liu, Ya‐Li Chen, Jie‐Lin Qin, Ting‐Ting Wang, Shan‐Shan FuMadison, WI, USA) and transfected with the control Renilla luciferase control reporter vector pRL‐TK (E2241, Promega) into HVSMCs stably transfected with sh‐RCOR1. After 48 h, the luciferase activity in cells

(82) B7-H3 CAR T-cells are effective against ependymomas, but limited by tumor size and immune responseClinical Cancer ResearchSeptember 2, 2025Sanya Mehta, Siri Ippagunta, Srinidhi Varadharajan, Alisha Kardian, Nic Laboe, Diana Dinh, Amber B. Jones, Michaela M. Meehl, Jorge Ibañez, Erik Emanus, et al.into D341 Med cells along with the pRL-TK Renilla Luciferase Control Reporter Vector (Promega, cat. # E2241) and ZFTA-RELA or GFP-encoding DNA plasmids using the standard GeneJuice (Fisher Scientific, cat. #

(83) Rational engineering of allosteric protein switches by in silico prediction of domain insertion sitesNat MethodsAugust 4, 2025Benedict Wolf, Pegi Shehu, Luca Brenker, Anna-Lisa von Bachmann, Ann-Sophie Kroell, Nicholas Southern, Stefan Holderbach, Joshua Eigenmann, Sabine Aschenbrenner, Jan Mathony, et al.Microsynth Seqlab. The Renilla luciferase expressing plasmid was obtained from Promega (pRL-TK, cat. no. E2241). pCAG-hMb3Cas12a-NLS(nucleoplasmin)-3xHA (RTW2500) was a gift from J. Bondy-Denomy, K. Joung and B.

(84) EDEM1 Inhibits Endoplasmic Reticulum Stress to Induce Doxorubicin Resistance through Accelerating ERAD and Activating Keap1/Nrf2 Antioxidant Pathway in Triple-Negative Breast CancerResearch (Wash D C)July 29, 2025Yajie Wang, Yiran Liang, Dan Luo, Fangzhou Ye, Yuhan Jin, Lei Wang, Yaming Li, Dianwen Han, Zekun Wang, Bing Chen, et al.was used as a template and was cloned into both pCMV-C-Myc and pCMV-C-HA vectors. Plasmids pRL-TK (E2241) and pmirGLO (E1330) were all from Promega (Madison, Wisconsin, USA). Hsa-miR-32-5p mimic, EDEM1 siRNA,

(85) TSC22D1 is a newly identified inhibitor of insulin secretion in pancreatic beta cellsThe FEBS JournalJuly 18, 2025Sümbül Yıldırım, Amit Mhamane, Svenja Lösch, Annika Wieder, Ezgi Ermis, Ann‐Christine König, Sevgican Yilmaz, Stefanie M. Hauck, Fatih Kocabas, Julia Szendroedi, et al.Addgene #49058, species mouse) [62] together with Renilla luciferase pRL‐TK (Promega; Madison, WI, USA; # E2241) plasmids using Lipofectamine™ 2000 Transfection Reagent (Invitrogen, #11668019) and OPTIMEM (Gibco,

(86) The Integrated Stress Response Pathway Coordinates Translational Control of Multiple Immune Checkpoints in Lung CancerCancer ResearchJuly 15, 2025Shayna Thomas-Jardin, Shruthy Suresh, Ariana Arce, Nicole Novaresi, Qing Deng, Emily Stein, Lisa Thomas, Cheryl Lewis, Chul Ahn, Bret M. Evers, et al.seeded/well in 12-well plates in triplicate and transfected with a Renilla plasmid (20 ng, Promega, E2241), firefly luciferase pGL3 plasmid (Promega, E1751, RRID: Addgene_212936) expressing CD155 WT 5′ UTR

(87) The Integrated Stress Response Pathway Coordinates Translational Control of Multiple Immune Checkpoints in Lung CancerCancer ResJuly 15, 2025Shayna Thomas-Jardin, Shruthy Suresh, Ariana Arce, Nicole Novaresi, Qing Deng, Emily Stein, Lisa Thomas, Cheryl Lewis, Chul Ahn, Bret M. Evers, et al.seeded/well in 12-well plates in triplicate and transfected with a Renilla plasmid (20ng, Promega, E2241), Firefly luciferase pGL3 plasmid (Promega, E1751, RRID:Addgene_212936) expressing CD155 wildtype 5′

(88) PKN2 Inhibits VEGFA and bFGF‐Mediated Angiogenesis by Targeting HIF‐1α in Colon CancerThe Kaohsiung Journal of Medical SciencesJuly 1, 2025Yun Zhu, Shi‐Yun Huang, Yi Lei, An‐Ni Luo, Xiang‐Zhao Li, Biao Wang, Peng‐Hui Sun, Si‐Tang Gong, Yang Chengtransfected with a luciferase reporter plasmid (200 ng) in combination with 80 ng pRL‐TK (Promega, E2241) using Lipofectamine 2000 (Invitrogen, 11668019). After 48 h of transfection, the medium was removed,

(89) B7-H3 CAR T Cells Are Effective against Ependymomas but Limited by Tumor Size and Immune ResponseClin Cancer ResJune 30, 2025Sanya Mehta, Siri Ippagunta, Srinidhi Varadharajan, Alisha Kardian, Nic Laboe, Diana Dinh, Amber B. Jones, Michaela M. Meehl, Jorge Ibañez, Erik Emanus, et al.into D341 Med cells along with the pRL-TK Renilla Luciferase Control Reporter Vector (Promega, cat. # E2241) and ZFTA-RELA or GFP-encoding DNA plasmids using the standard GeneJuice (Fisher Scientific, cat. #

(90) MYC promotes group 3 medulloblastoma cell proliferation and alleviates ROS-induced cell death by upregulating transketolaseActa Neuropathol CommunJune 28, 2025Shizun Wang, Dan Zhang, Chunlong Wang, Yuqin Liudensity of 5 × 10⁵ cells per well and co-transfected with the recombinant pGL4.10 plasmid, pRL-TK vector (E2241, Promega, WI, USA), and (or) overexpression plasmids using Lipofectamine™ 3000 (L3000015, Thermo Fisher,

(91) The regulatory variant rs1950834 confers the risk of depressive disorder by reducing LRFN5 expressionBMC MedMay 30, 2025Di Luan, Yifan Li, Aini Zhang, Qingqing Bai, Te Zhao, Xi Chen, Xinglun Dang, Junyang Wang, Shaolei Jiang, Yun Sun, et al.reporter vectors containing the cloned fragments and control pRL-TK vectors (Renilla Luciferase) (Promega, E2241) were co-transfected into cultured cells using Lipofectamine 3000 reagent (ThermoFisher, L3000015).

(92) Inhibitory effects of β-galactoside α2,6-sialyltransferase 1 on the Hippo pathway in breast cancer cellsJournal of Biological ChemistryMay 21, 2025Qinglei Hang, Wenqian Li, Jingya Guo, Shiying Zuo, Yawen Yang, Can Wu, Wen Yong, Caimin Li, Jianguo Gu, Sicong HouGTIIC luciferase reporter (Addgene plasmid ID: 34615) and a TK-Renilla luciferase reporter (Promega, #E2241). 48 h after transfection, the reporter activities were measured using a Dual-Luciferase Reporter Assay system

(93) Cell-intrinsic regulation of HBV RNAs by the nonsense-mediated mRNA decay pathway controls viral replicationiScienceMay 16, 2025Masami Wada, Chiharu Morita, Eriko Ohsaki, Keiji Uedapaper,N/A pT7-2.1kb RNA,This paper,N/A pT7-Small S RNA,This paper,N/A pT7-XRNA,This paper,N/A pRL-TK,Promega,Cat#E2241 pcDNA-GFP,Nakayama et al.48,N/A pHBV-WT,Fauzyah et al.46 Sugiyama et al.47,N/A pHBV pol dead,This

(94) U2AF65 mediated circPVT1 promotes NSCLC cell proliferation and inhibits ferroptosis through the miR-338-3p/GPX4 axisCell Biol ToxicolMay 14, 2025Lujuan He, Zezhi Zhou, Jufen Wang, Jiehan Jiang, Shenggang LiuWI, USA) and 50 nM miR-338-3p mimic (HY-R00698, MCE, NJ, USA). Renilla luciferase plasmid pRL-TK (#E2241, Promega) served as an internal control. Transfection was performed utilizing Lipofectamine® 2000 (#11668019,

(95) Microtubule associated serine/threonine kinase-3 inhibits the malignant phenotype of breast cancer by promoting phosphorylation-mediated ubiquitination degradation of yes-associated proteinBreast Cancer ResMay 1, 2025Ning Deng, Wei Kang, Jiang Du, Xuezhu Rong, Qiang Hanluciferase plasmid (# 34615) was purchased from Addgene (Cambridge, MA, USA). The pRL-TK vector (# E2241) was purchased from Promega (Madison, Wisconsin, USA). shYAP (# sc-38637-SH) was purchased from Santa Cruz Biotechnology, Inc

(96) Polycyclic aromatic hydrocarbons promote tumorigenesis of gallbladder cancer via Aryl Hydrocarbon Receptor-HEGBC positive feedback axisiScienceApril 23, 2025Lei Yuan, Yumeng Zhang, Jianguo Xu, Feng Liu, Yingchao Liang, Beili Wang, Meihong Wu, Zhiyuan Fan, Liang Yangin their studies of AhR activation.39,40 A renilla luciferase construct control vector (pRLTK; Cat#E2241, Promega, Wisconsin, USA) was transfected into primary NHEKs with TransIT Keratinocyte transfection

(97) A blocking antibody against anti-Müllerian hormone restores ovulation and normal androgen levels in a spontaneous rat model of polycystic ovary syndromeEBioMedicineApril 18, 2025Chrystèle Racine, François Fraissinet, Stefania Tolu, Tony Pereira, Stéphanie Gil, Anne Badel, Danielle Bailbé, Bruno Fève, Jamileh Movassat, Richard Cate, et al.Gal4-Smad5 and Gal4-luc plasmids or Cyp19a1 or StAR reporter genes, and 12.5 ng/w of pRLTK vector (E2241, Promega, Madison, WI) as a control for transfection efficiency. For KK1 cells, the mouse AMHR2 cDNA

(98) A nuclear RNA degradation code is recognized by PAXT for eukaryotic transcriptome surveillanceMolecular CellApril 17, 2025Lindsey V. Soles, Liang Liu, Xudong Zou, Yoseop Yoon, Shuangyu Li, Lusong Tian, Marielle Valdez, Angela M Yu, Hong Yin, Wei Li, et al.pX330A-S-ZFC3H1-sgRNA,This study,N/A Plasmid: pGL3-promoter,Promega,Cat#E1751 Plasmid: pRL-TK,Promega,Cat#E2241 Plasmid: pCDNA5: FLAG-eGFP-5′ ss WT-bGH PAS,This study,N/A Plasmid: pCDNA5: FLAG-eGFP-5′ ss Mut-bGH

(99) High-Throughput Screening to Identify Novel Compounds Affecting the Genome Editing Efficiency of CRISPR SystemMoleculesApril 17, 2025Jiasong Chang, Xiulong Yang, Tong Zhang, Hao Sun, Hongying Cheng, Zhangrong Jia, Yiying Li, Sanyuan Ma, Teng Sun, Jimin Caothe SSA reporter vector, named T-CMV-SSA-luciferase. The Renilla luciferase expression vector pRL-TK (E2241) was purchased from Promega (Madison, WI, USA). The CRISPR vector pX330-U6-Chimeric_BB-CBh-hSpCas9 was

(100) Inhibition of Rho GEFs attenuates pulmonary fibrosis through suppressing myofibroblast activation and reprogramming profibrotic macrophagesCell Death DisApril 11, 2025Chengju Luo, Chenqi Huang, Yuqi Zhu, Yuxin Zhou, Yansheng Qiao, Chenxiao Shi, Yuan Gao, Yongjian Guo, Libin Weitransfected with 2 μg of 8xGTIIC-luciferase plasmid (Addgene, #34615) and 0.2 μg of pRL-TK plasmid (Promega, E2241) using jetPRIME transfection reagent (Polyplus, France). After drug treatment, Firefly luciferase and

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pRL-TK Vector

pRL-TK Vector from Promega

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Specifications/Features

Promega

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Reviews

pRL-TK Vector from Promega

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