pRL-TK Vector from Promega

Product Specs
ItempRL-TK Vector
CompanyPromega
Price $0.00Supplier Page View Company Product Page
Catalog NumberE2241
HostMammalian
PromoterHSV TK promoter
Vector NamepRL-TK
Quantity20microg
ReporterRenilla luciferase
ResistanceAmpR
Molecular Weight~73kDa
Selectable MarkerAmpicillin

Specifications/Features

Vector Size:
4045 bp

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(81) SOX11-mediated CBLN2 Upregulation Contributes to Neuropathic Pain through NF-κB-Driven Neuroinflammation in Dorsal Root Ganglia of MiceNeuroscience BulletinOctober 29, 2025Ling-Jie Ma, Tian Wang, Ting Xie, Lin-Peng Zhu, Zuo-Hao Yao, Meng-Na Li, Bao-Tong Yuan, Xiao-Bo Wu, Yong-Jing Gao, Yi-Bin Qinand control plasmids were transfected into HEK293T cells along with the pRL-TK plasmid (Promega, USA, E2241) as an internal control. Fluorescence was measured after 48 h following the instructions of Dual-Luciferase® Reporter Assay System

(82) Deacetylation of TALDO1 by HDAC6 promotes glycolysis and nasopharyngeal carcinoma progression through a moonlighting functionCell Death DisOctober 21, 2025Xingzhi Peng, Peijun Zhou, Kun Zhang, Likang Chen, Min Tang, Qin Zhou, Jinwu Peng, Lifang Yangluciferase reporter vector pGL3-Basic (E1751) and Renilla luciferase reporter gene control vector pRL-TK (E2241) were obtained from Promega (Madison, WI). pcDNA3-HDAC6-DN-Flag plasmid was provided by Professor Wei

(83) The miR-34/449 clusters regulate the differentiation of ciliated cells in the oviduct via the Wnt/β-catenin signaling pathwayCell BiosciOctober 2, 2025Qiqian Wu, Yunxia Zhang, Furong Bai, Yujie Wu, Yanqin Hu, Jingwen Wucatalog no. 12456, USA) or FOPflash (Addgene, catalog no. 12457, USA), and pRL-TK (Promega, catalog no. E2241, USA). The Dual-Luciferase Reporter Assay System (Promega, catalog no. E1910, USA) was used to measure

(84) Elevated NEGR1 in brain induces anxiety or depression-like phenotypes and synaptic dysfunctionMolecular PsychiatryOctober 1, 2025Ya-Qi Zhang, Qing Zhang, Yi Yang, Li-Li Yu, Ning-Lin Fan, Yong Wu, Jun-Yang Wang, Xing-Lun Dang, Ying-Qi Guo, Cong Li, et al.restriction enzyme sites by SYNBIOB GENE company. The pGL4.10, pGL4.10-rs3101339-A or -C, and pRL-TK (#E2241, Promega) vectors were verified by Sanger sequencing and amplified for experimental plasmid preparation.

(85) Genotypic, functional, and phenotypic characterization in CTNNB1 neurodevelopmental syndromeHuman Genetics and Genomics AdvancesOctober 1, 2025Nina Žakelj, David Gosar, Špela Miroševič, Stephan J. Sanders, Alicia Ljungdahl, Sayeh Kohani, Shouhe Huang, Lok I Leong, Ying An, Miou-Jing Teo, et al.Promega, Madison, WI, USA) was carried out as described previously.1 The pTK-RL Renilla luciferase vector (E2241, Promega) was used as an internal control reporter construct for the normalization of transfection efficiency.

(86) Local Growth Hormone Facilitates Aging of the Colon Epithelial MicroenvironmentAging cellOctober 1, 2025Chesnokova V, Zonis S, Ainsworth R, Apaydin T, Valencia CW, Greiner EC, Barrett R, Kettenbach AN, Melmed S.plasmid together with 0.1 μg pRL‐TK Renilla Luciferase Control Reporter Vector (Promega Corporation, cat#E2241) using Amaxa nucleofector I (Lonza) and Amaxa Basic Nucleofector Kit for Primary Mammalian Epithelial Cells

(87) Modification of adipose mass by targeting distal enhancers of PtrfBMC BiologySeptember 30, 2025Yu Zhang, Keren Long, Songling Zhang, Biwei Zhang, Xiaokai Li, Xuemin Li, Zhining Zhong, Ziqi Li, Qiuyue Chen, Penghao Li, et al.detect putative enhancer activity in vitro. Then, 100 ng pGL3-promoter-enhancer and 2 ng TK-15 (Promega, E2241) were transfected into HEK293 and 3T3-AD in 96-well plates using Lipofectamine 3000 reagents (ThermoFisher,

(88) NAT10 Suppresses RNA Sensing Induced IFN-β Transactivation to Promote Viral Infection via Interfering with IRF3 ActivitiesbioRxivSeptember 11, 2025Youngmin Park, Jinshan He, Eleya Eleya, Zhenyu Zhenyu, Guillaume N Fiches, Dawei Zhou, Emily G Watters, Zhixian M He, Kateepe ASN Shanaka, Thurbu T Lepcha, et al.pGL3-IFN-β-luciferase reporter vector (Cat. # 102597, Addgene) and pRL-TK renilla luciferase vector (Cat. # E2241, Promega) along with Ptrip-SFFV-mtagBFP-2A RIGI WT (Cat. # 167289, Addgene) using the TurboFect™ transfection reagent

(89) Rosuvastatin Upregulates RCOR1 to Repress C10ORF10 Transcription and Alleviate Oxidative Stress and Plaque Formation in AtherosclerosisThe Kaohsiung Journal of Medical SciencesSeptember 9, 2025Xin Fu, Chuang Liu, Ya‐Li Chen, Jie‐Lin Qin, Ting‐Ting Wang, Shan‐Shan FuMadison, WI, USA) and transfected with the control Renilla luciferase control reporter vector pRL‐TK (E2241, Promega) into HVSMCs stably transfected with sh‐RCOR1. After 48 h, the luciferase activity in cells

(90) B7-H3 CAR T-cells are effective against ependymomas, but limited by tumor size and immune responseClinical Cancer ResearchSeptember 2, 2025Sanya Mehta, Siri Ippagunta, Srinidhi Varadharajan, Alisha Kardian, Nic Laboe, Diana Dinh, Amber B. Jones, Michaela M. Meehl, Jorge Ibañez, Erik Emanus, et al.into D341 Med cells along with the pRL-TK Renilla Luciferase Control Reporter Vector (Promega, cat. # E2241) and ZFTA-RELA or GFP-encoding DNA plasmids using the standard GeneJuice (Fisher Scientific, cat. #

(91) Rational engineering of allosteric protein switches by in silico prediction of domain insertion sitesNat MethodsAugust 4, 2025Benedict Wolf, Pegi Shehu, Luca Brenker, Anna-Lisa von Bachmann, Ann-Sophie Kroell, Nicholas Southern, Stefan Holderbach, Joshua Eigenmann, Sabine Aschenbrenner, Jan Mathony, et al.Microsynth Seqlab. The Renilla luciferase expressing plasmid was obtained from Promega (pRL-TK, cat. no. E2241). pCAG-hMb3Cas12a-NLS(nucleoplasmin)-3xHA (RTW2500) was a gift from J. Bondy-Denomy, K. Joung and B.

(92) EDEM1 Inhibits Endoplasmic Reticulum Stress to Induce Doxorubicin Resistance through Accelerating ERAD and Activating Keap1/Nrf2 Antioxidant Pathway in Triple-Negative Breast CancerResearch (Wash D C)July 29, 2025Yajie Wang, Yiran Liang, Dan Luo, Fangzhou Ye, Yuhan Jin, Lei Wang, Yaming Li, Dianwen Han, Zekun Wang, Bing Chen, et al.was used as a template and was cloned into both pCMV-C-Myc and pCMV-C-HA vectors. Plasmids pRL-TK (E2241) and pmirGLO (E1330) were all from Promega (Madison, Wisconsin, USA). Hsa-miR-32-5p mimic, EDEM1 siRNA,

(93) TSC22D1 is a newly identified inhibitor of insulin secretion in pancreatic beta cellsThe FEBS JournalJuly 18, 2025Sümbül Yıldırım, Amit Mhamane, Svenja Lösch, Annika Wieder, Ezgi Ermis, Ann‐Christine König, Sevgican Yilmaz, Stefanie M. Hauck, Fatih Kocabas, Julia Szendroedi, et al.Addgene #49058, species mouse) [62] together with Renilla luciferase pRL‐TK (Promega; Madison, WI, USA; # E2241) plasmids using Lipofectamine™ 2000 Transfection Reagent (Invitrogen, #11668019) and OPTIMEM (Gibco,

(94) The Integrated Stress Response Pathway Coordinates Translational Control of Multiple Immune Checkpoints in Lung CancerCancer ResearchJuly 15, 2025Shayna Thomas-Jardin, Shruthy Suresh, Ariana Arce, Nicole Novaresi, Qing Deng, Emily Stein, Lisa Thomas, Cheryl Lewis, Chul Ahn, Bret M. Evers, et al.seeded/well in 12-well plates in triplicate and transfected with a Renilla plasmid (20 ng, Promega, E2241), firefly luciferase pGL3 plasmid (Promega, E1751, RRID: Addgene_212936) expressing CD155 WT 5′ UTR

(95) The Integrated Stress Response Pathway Coordinates Translational Control of Multiple Immune Checkpoints in Lung CancerCancer ResJuly 15, 2025Shayna Thomas-Jardin, Shruthy Suresh, Ariana Arce, Nicole Novaresi, Qing Deng, Emily Stein, Lisa Thomas, Cheryl Lewis, Chul Ahn, Bret M. Evers, et al.seeded/well in 12-well plates in triplicate and transfected with a Renilla plasmid (20ng, Promega, E2241), Firefly luciferase pGL3 plasmid (Promega, E1751, RRID:Addgene_212936) expressing CD155 wildtype 5′

(96) PKN2 Inhibits VEGFA and bFGF‐Mediated Angiogenesis by Targeting HIF‐1α in Colon CancerThe Kaohsiung Journal of Medical SciencesJuly 1, 2025Yun Zhu, Shi‐Yun Huang, Yi Lei, An‐Ni Luo, Xiang‐Zhao Li, Biao Wang, Peng‐Hui Sun, Si‐Tang Gong, Yang Chengtransfected with a luciferase reporter plasmid (200 ng) in combination with 80 ng pRL‐TK (Promega, E2241) using Lipofectamine 2000 (Invitrogen, 11668019). After 48 h of transfection, the medium was removed,

(97) B7-H3 CAR T Cells Are Effective against Ependymomas but Limited by Tumor Size and Immune ResponseClin Cancer ResJune 30, 2025Sanya Mehta, Siri Ippagunta, Srinidhi Varadharajan, Alisha Kardian, Nic Laboe, Diana Dinh, Amber B. Jones, Michaela M. Meehl, Jorge Ibañez, Erik Emanus, et al.into D341 Med cells along with the pRL-TK Renilla Luciferase Control Reporter Vector (Promega, cat. # E2241) and ZFTA-RELA or GFP-encoding DNA plasmids using the standard GeneJuice (Fisher Scientific, cat. #

(98) MYC promotes group 3 medulloblastoma cell proliferation and alleviates ROS-induced cell death by upregulating transketolaseActa Neuropathol CommunJune 28, 2025Shizun Wang, Dan Zhang, Chunlong Wang, Yuqin Liudensity of 5 × 10⁵ cells per well and co-transfected with the recombinant pGL4.10 plasmid, pRL-TK vector (E2241, Promega, WI, USA), and (or) overexpression plasmids using Lipofectamine™ 3000 (L3000015, Thermo Fisher,

(99) The regulatory variant rs1950834 confers the risk of depressive disorder by reducing LRFN5 expressionBMC MedMay 30, 2025Di Luan, Yifan Li, Aini Zhang, Qingqing Bai, Te Zhao, Xi Chen, Xinglun Dang, Junyang Wang, Shaolei Jiang, Yun Sun, et al.reporter vectors containing the cloned fragments and control pRL-TK vectors (Renilla Luciferase) (Promega, E2241) were co-transfected into cultured cells using Lipofectamine 3000 reagent (ThermoFisher, L3000015).

(100) Inhibitory effects of β-galactoside α2,6-sialyltransferase 1 on the Hippo pathway in breast cancer cellsJournal of Biological ChemistryMay 21, 2025Qinglei Hang, Wenqian Li, Jingya Guo, Shiying Zuo, Yawen Yang, Can Wu, Wen Yong, Caimin Li, Jianguo Gu, Sicong HouGTIIC luciferase reporter (Addgene plasmid ID: 34615) and a TK-Renilla luciferase reporter (Promega, #E2241). 48 h after transfection, the reporter activities were measured using a Dual-Luciferase Reporter Assay system

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pRL-TK Vector

pRL-TK Vector from Promega

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Specifications/Features

Promega

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pRL-TK Vector from Promega

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