pRL-TK Vector from Promega

Product Specs
ItempRL-TK Vector
CompanyPromega
Price $0.00Supplier Page View Company Product Page
Catalog NumberE2241
HostMammalian
PromoterHSV TK promoter
Vector NamepRL-TK
Quantity20microg
ReporterRenilla luciferase
ResistanceAmpR
Molecular Weight~73kDa
Selectable MarkerAmpicillin

Specifications/Features

Vector Size:
4045 bp

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(61) Divergent viral phosphodiesterases for immune signaling evasionCell Host & MicrobeDecember 10, 2025Erin E. Doherty, Jason Nomburg, Benjamin A. Adler, Santiago Lopez, Kendall Hsieh, Nathan Price, Nurashau Blount, Jennifer A. Doudnadriven by an IFNB promoter (IFN-Beta_pGL3, Addgene 102597), 5 ng Renilla luciferase (pRL-TK, Promega E2241), and 20 ng of each putative PDE using the Mirus TransITX2 transfection reagent. After at least 4 h,

(62) Lactylation-driven KRT19 promotes non-small cell lung cancer progression by suppressing cellular senescenceJournal of Experimental & Clinical Cancer ResearchDecember 4, 2025Cai Zhang, Yue Du, Yangyang Ji, Xiaoxiao Ye, Jingyao Lian, Haonan Zhou, Zihan Gao, Huiping Xu, Yuehan Tang, Yanhong Fan, et al.(PGL3-KRT19 vector). A549 and PC-9 cells were co-transfected with pGL3-basic or pGL3-KRT19-Luc and pRL-TK (E2241, Promega) for 48 h. Then, A549 cells were incubated with lactate (40 mM) or vehicle for 16 h, PC-9 cells

(63) ER stress-induced ITPR1/ANO1 signaling drives trigeminal neuropathic pain through calcium-dependent neuronal hyperexcitabilityJ Headache PainDecember 1, 2025Huan-Jun Lu, Jia-Wen Shen, Ning-Yi Ren, Nan Hu, Jian-Ke Cen, Meng-Xuan Huang, Si-Yuan Song, Ling-Jie Ma, Xiao-Bo Wu, Xia Li, et al.were transfected with treatment and control plasmids, along with the pRL-TK plasmid (Promega, USA, E2241) serving as an internal standard following a method previously described [38]. The over-expression plasmid

(64) Histone demethylase KDM9 activates the CCND1/AKT pathway to promote the malignant progression of gliomas through interaction with BRD2Cellular and Molecular Life SciencesNovember 26, 2025Jin Cheng, Xingzhi Peng, Siyuan Tang, Peijun Zhou, Zhuan Zhou, Zenghui Mao, Dan Li, Lifang Yang, Jinwu Pengluciferase reporter vector pGL3-Basic (E1751) and renilla luciferase reporter gene control vector pRL-TK (E2241) were obtained from Promega (Madison, WI). The ApaI (1604, Takara, Tokyo, Japan) and EcoRI (1611, Takara)

(65) A luminescence-based biosensor to measure endogenous UBE3A activityiScienceNovember 21, 2025Lei Xing, Sophia U. Lamberti, Hannah C. Nourie, Trinity E. Rust, Wenxin Hu, Mark J. Zylkaluciferase-expressing construct driven by herpes simplex virus HSV-thymidine kinase (TK) promoter (pRL-TK, #E2241) were purchased from Promega. Firefly-luciferase coding sequence was amplified from BAR-pGL3 vector10

(66) UHRF1 restricts HCoV-229E infection through epigenetic silencing of the viral receptor APNNature CommunicationsNovember 18, 2025Pengcheng Wang, Ziqiao Wang, Fei Feng, Lisha Yin, Yuyuan Zhang, Zhichao Gao, Jiannan Chen, Ping Zhang, Shuiqiao Yuan, Qiang Ding, et al.unmethylated or methylated luciferase reporter plasmid pGL3-APN-Luc and 50 ng of pRL-TK plasmid (Promega # E2241). Luciferase activity was measured at 24 h post-transfection using the Dual-Luciferase Reporter Assay System

(67) OSA Initiates Histone Lactylation That Drives PDE4B / FUS / AGT Axis to Pulmonary HypertensionCell ProliferationNovember 17, 2025Li Yang, Qing Ni, Yan He, Shijie Liu, Lulu Gan, Anni Dai, Yang Hu, Qian Liu, Xueling Yang, Jiqian Li, et al.500 ng reporter plasmid (pGL4.10[luc2], Promega, E6651) and 50 ng pRL‐TK (Renilla control, Promega, E2241) using Lipofectamine 3000 (Invitrogen, L3000001). 2.18.2 Dual‐Luciferase Assay Forty‐eight hours post‐transfection,

(68) Endogenous fine-mapping and prioritization of functional regulatory elements in complex genetic lociCell GenomNovember 12, 2025Zhao K, Zhou Y, Wang X, Wang Z, Xu X, Chen Y, Zhao L, Wu C, Wang J, Yao H, et al.al.87",RRID:Addgene_48138 pGL3-basic,Promega,Cat#E1751 pGL3-promoter,Promega,Cat#E1761 pRL-TK Renilla,Promega,Cat#E2241 ,, Software and algorithms,Software and algorithms,Software and algorithms ,, GraphPad Prism,N/A,https://www.graphpad.com/features FastQTL,"Ongen,

(69) PCBP2 Regulates p16 INK4a ‐Dependent Cellular Senescence in Response to IronAging CellNovember 11, 2025Shaoyang Yan, Lili Lu, Yuwei Wu, Zewen Du, Ebin Johny, Partha Dutta, Gang LiAdditionally, we transfected the same amounts of an internal control vector, pRL‐TK (Promega, Cat#: E2241), which constitutively expressed the Renilla luciferase. Relative luciferase reporter activities were

(70) Deciphering oligomeric proanthocyanidins’ dual osteoprotective mechanisms at single-cell resolution: NR4A1-mediated PTGS2 suppression and β-catenin-Runx2 activationFrontiers in ImmunologyNovember 4, 2025Li Huang, Yuwei Sun, Yi Zheng, Shicheng Qiu, Jianping Zheng, Chunhan Sun, Mingwei Chen, Shaowei Zheng, Yirong Zengplasmid and 100 ng of pRL-TK Renilla luciferase vector (Promega Corporation, Madison, WI, USA, Cat. No. E2241) as an internal control via Lipofectamine 3000 (Thermo Fisher Scientific, Waltham, MA, USA, Cat. No.

(71) SAG1.3-derived Frizzled-targeting small-molecule compoundsJournal of Biological ChemistryNovember 1, 2025Lukas Grätz, Ainoleena Turku, Pawel Kozielewicz, Carl-Fredrik Bowin, Magdalena M. Scharf, Jan H. Voss, Julia Kinsolving, Rawan Shekhani, Nuria Oliva-Vilarnau, Tobias Koolmeister, et al.sequence for the human TK promoter (note: weak promoter sequence) was amplified from pRL-TK (Promega; E2241) and used to replace the cytomegalovirus promoter present in the original FZD5–DEP–Clamp plasmid (26)

(72) SOX11-mediated CBLN2 Upregulation Contributes to Neuropathic Pain through NF-κB-Driven Neuroinflammation in Dorsal Root Ganglia of MiceNeuroscience BulletinOctober 29, 2025Ling-Jie Ma, Tian Wang, Ting Xie, Lin-Peng Zhu, Zuo-Hao Yao, Meng-Na Li, Bao-Tong Yuan, Xiao-Bo Wu, Yong-Jing Gao, Yi-Bin Qinand control plasmids were transfected into HEK293T cells along with the pRL-TK plasmid (Promega, USA, E2241) as an internal control. Fluorescence was measured after 48 h following the instructions of Dual-Luciferase® Reporter Assay System

(73) Sexual dimorphism in colon is mediated by an androgen-IL33+ stromal cell axisCell BiosciOctober 29, 2025Haoyu Wang, Baowei Jing, Juan Zou, Tian Lan, Mengxin Hu, Lan Lin, Hanhua Cheng, Rongjia Zhoueach well of a 48-well plate were transfected with 0.5 µg recombinant constructs and 10 ng pRL-TK (E2241, Promega). Luciferase activities were subsequently measured using the dual-luciferase reporter assay

(74) Deacetylation of TALDO1 by HDAC6 promotes glycolysis and nasopharyngeal carcinoma progression through a moonlighting functionCell Death DisOctober 21, 2025Xingzhi Peng, Peijun Zhou, Kun Zhang, Likang Chen, Min Tang, Qin Zhou, Jinwu Peng, Lifang Yangluciferase reporter vector pGL3-Basic (E1751) and Renilla luciferase reporter gene control vector pRL-TK (E2241) were obtained from Promega (Madison, WI). pcDNA3-HDAC6-DN-Flag plasmid was provided by Professor Wei

(75) The miR-34/449 clusters regulate the differentiation of ciliated cells in the oviduct via the Wnt/β-catenin signaling pathwayCell BiosciOctober 2, 2025Qiqian Wu, Yunxia Zhang, Furong Bai, Yujie Wu, Yanqin Hu, Jingwen Wucatalog no. 12456, USA) or FOPflash (Addgene, catalog no. 12457, USA), and pRL-TK (Promega, catalog no. E2241, USA). The Dual-Luciferase Reporter Assay System (Promega, catalog no. E1910, USA) was used to measure

(76) Local Growth Hormone Facilitates Aging of the Colon Epithelial MicroenvironmentAging cellOctober 1, 2025Chesnokova V, Zonis S, Ainsworth R, Apaydin T, Valencia CW, Greiner EC, Barrett R, Kettenbach AN, Melmed S.plasmid together with 0.1 μg pRL‐TK Renilla Luciferase Control Reporter Vector (Promega Corporation, cat#E2241) using Amaxa nucleofector I (Lonza) and Amaxa Basic Nucleofector Kit for Primary Mammalian Epithelial Cells

(77) Genotypic, functional, and phenotypic characterization in CTNNB1 neurodevelopmental syndromeHuman Genetics and Genomics AdvancesOctober 1, 2025Nina Žakelj, David Gosar, Špela Miroševič, Stephan J. Sanders, Alicia Ljungdahl, Sayeh Kohani, Shouhe Huang, Lok I Leong, Ying An, Miou-Jing Teo, et al.Promega, Madison, WI, USA) was carried out as described previously.1 The pTK-RL Renilla luciferase vector (E2241, Promega) was used as an internal control reporter construct for the normalization of transfection efficiency.

(78) Elevated NEGR1 in brain induces anxiety or depression-like phenotypes and synaptic dysfunctionMolecular PsychiatryOctober 1, 2025Ya-Qi Zhang, Qing Zhang, Yi Yang, Li-Li Yu, Ning-Lin Fan, Yong Wu, Jun-Yang Wang, Xing-Lun Dang, Ying-Qi Guo, Cong Li, et al.restriction enzyme sites by SYNBIOB GENE company. The pGL4.10, pGL4.10-rs3101339-A or -C, and pRL-TK (#E2241, Promega) vectors were verified by Sanger sequencing and amplified for experimental plasmid preparation.

(79) Modification of adipose mass by targeting distal enhancers of PtrfBMC BiologySeptember 30, 2025Yu Zhang, Keren Long, Songling Zhang, Biwei Zhang, Xiaokai Li, Xuemin Li, Zhining Zhong, Ziqi Li, Qiuyue Chen, Penghao Li, et al.detect putative enhancer activity in vitro. Then, 100 ng pGL3-promoter-enhancer and 2 ng TK-15 (Promega, E2241) were transfected into HEK293 and 3T3-AD in 96-well plates using Lipofectamine 3000 reagents (ThermoFisher,

(80) NAT10 Suppresses RNA Sensing Induced IFN-β Transactivation to Promote Viral Infection via Interfering with IRF3 ActivitiesbioRxivSeptember 11, 2025Youngmin Park, Jinshan He, Eleya Eleya, Zhenyu Zhenyu, Guillaume N Fiches, Dawei Zhou, Emily G Watters, Zhixian M He, Kateepe ASN Shanaka, Thurbu T Lepcha, et al.pGL3-IFN-β-luciferase reporter vector (Cat. # 102597, Addgene) and pRL-TK renilla luciferase vector (Cat. # E2241, Promega) along with Ptrip-SFFV-mtagBFP-2A RIGI WT (Cat. # 167289, Addgene) using the TurboFect™ transfection reagent

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pRL-TK Vector

pRL-TK Vector from Promega

Description

Specifications/Features

Promega

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pRL-TK Vector from Promega

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