pRL-TK Vector from Promega

Product Specs
ItempRL-TK Vector
CompanyPromega
Price $0.00Supplier Page View Company Product Page
Catalog NumberE2241
HostMammalian
PromoterHSV TK promoter
Vector NamepRL-TK
Quantity20microg
ReporterRenilla luciferase
ResistanceAmpR
Molecular Weight~73kDa
Selectable MarkerAmpicillin

Specifications/Features

Vector Size:
4045 bp

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(61) Functional variants at 1p36.23 confer risk of schizophrenia through modulating RERENature CommunicationsJanuary 24, 2026Yixing Liu, Junyang Wang, Hong Yang, Yifan Li, Changgai Mu, Xinglun Dang, Xinran Chen, Danyang Li, Mingna Li, Jiewei Liu, et al.of plating, 100 ng of recombinant vector and 20 ng of the pRL-TK vector (internal control; Promega, E2241) were transfected into HEK-293T cells. For SH-SY5Y and U251 cells, 150 ng of recombinant vector and

(62) The castration-resistant prostate cancer-associated SNP rs11067228 facilitates neuroendocrine differentiation through an enhancer-mediated chromatin interaction with SRRM4International Journal of Biological SciencesJanuary 8, 2026Yuan Jiang, Zhenhao Zhao, Peng Li, Guangsong Su, Yuyang Qian, Yuting Zhao, Bo Wang, Yunlong Bai, Lei Zhang, Zhongfang Zhao, et al.plates and cultured overnight. Reporter plasmids and the pRL-TK Renilla luciferase control vector (E2241, Promega) were then co-transfected into 22Rv1 cells using Lipofectamine 3000 reagent (Invitrogen). Cells

(63) A fibroblast-like endothelial cell state promotes atherosclerosis via C/EBPβ-activated TGF-β signalingThe EMBO JournalJanuary 6, 2026Linge Fan, Yingyi Zhu, Yi Li, Zixin Ji, Kefan Ma, Ying Zhang, Leiting Wei, Junbo Chen, Yuanqing Jiang, Dongwu Lai, et al.luciferase reporter vector, 1 μg of empty vector or CEBPB overexpression vector and 0.2 μg pRL-TK (Promega, E2241). Cells were cultured for 48 h and detected using the Dual Luciferase Reporter Gene Assay Kit (YEASEN,

(64) Multidimensional mapping of stimulation-responsive regulatory elements and candidate causal variants in T cell activationSci AdvJanuary 1, 2026Zhao Wang, Wei Dong, Xiangling Feng, Xinran Xu, Yao An, Huiling Xiao, Menghan Luo, Qian Liang, Ruoxuan Bai, Ke Zhao, et al.of the empty vector) and cotransfected with 20 ng of the pRL-TK Renilla Luciferase vector (Promega, E2241) into 293T cells in 24-well plates using the Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific

(65) Drynachromoside A from Drynaria fortunei attenuates oxidative stress and fibrosis of diabetic nephropathy via activating Nrf2 in vitro and in vivoRedox ReportDecember 31, 2025Huankai Yao, Jindong Li, Ruiqing Wu, Dan Du, Fengwei Ai, Yan Li(pGL4.37[luc2P/ARE/Hygro]) (Cat. no. E3641), Renilla luciferase reporter plasmid (pRL-TK) (Cat. no. E2241) and dual-luciferase reporter assay system (Cat. no. E1910) were obtained from Promega (Madison, WI,

(66) KLF13-Mediated CES2 Upregulation via p300-Dependent Acetylation Sensitizes Gastric Cancer Cells to IrinotecaniScienceDecember 19, 2025Hai-bin Zhang, Ren-hao Hu, Ke-hui Zhang, Xi-mao Cui, Shun Zhangvector,Sigma-Aldrich,Cat#E2150 pGL4.10[luc2] vector,Promega,Cat# E6651 pRL-TK vector,Promega,Cat# E2241 KLF13-pFLAG expression plasmid,This paper,N/A "KLF13 mutant plasmids (K226R/227R, K226Q/227Q)",This

(67) Regulation of glypican 6-mediated Wnt activation maintains TDP-43 nuclear localization in neuronsScientific ReportsDecember 11, 2025Nan Zhang, Shanthini Sockanathaninformation. TOPFlash luciferase assay Renilla luciferase control construct (pRL-TK) was purchased from Promega (E2241). Dual-luciferase reporter assay was performed according to the manufacturer’s instructions (Promega,

(68) Nucleolar MED19 regulates 2'-O-methylation of rRNA in supporting cancer cell growthNucleic Acids ResDecember 11, 2025Yang Ming, Zhao Jiamin, Peng Yu, Lu Chengjiang, Zhang Yiyang, Zhang Hanqing, Qiang Zhou, Gang Wangsequence into the 5′ end of the Rennila luciferase sequence in the original pRL-TK plasmid (Promega, #E2241). All the constructs were verified by Sanger sequencing. All the oligonucleotides were synthesized by

(69) Divergent viral phosphodiesterases for immune signaling evasionCell Host & MicrobeDecember 10, 2025Erin E. Doherty, Jason Nomburg, Benjamin A. Adler, Santiago Lopez, Kendall Hsieh, Nathan Price, Nurashau Blount, Jennifer A. Doudnadriven by an IFNB promoter (IFN-Beta_pGL3, Addgene 102597), 5 ng Renilla luciferase (pRL-TK, Promega E2241), and 20 ng of each putative PDE using the Mirus TransITX2 transfection reagent. After at least 4 h,

(70) Lactylation-driven KRT19 promotes non-small cell lung cancer progression by suppressing cellular senescenceJournal of Experimental & Clinical Cancer ResearchDecember 4, 2025Cai Zhang, Yue Du, Yangyang Ji, Xiaoxiao Ye, Jingyao Lian, Haonan Zhou, Zihan Gao, Huiping Xu, Yuehan Tang, Yanhong Fan, et al.(PGL3-KRT19 vector). A549 and PC-9 cells were co-transfected with pGL3-basic or pGL3-KRT19-Luc and pRL-TK (E2241, Promega) for 48 h. Then, A549 cells were incubated with lactate (40 mM) or vehicle for 16 h, PC-9 cells

(71) ER stress-induced ITPR1/ANO1 signaling drives trigeminal neuropathic pain through calcium-dependent neuronal hyperexcitabilityJ Headache PainDecember 1, 2025Huan-Jun Lu, Jia-Wen Shen, Ning-Yi Ren, Nan Hu, Jian-Ke Cen, Meng-Xuan Huang, Si-Yuan Song, Ling-Jie Ma, Xiao-Bo Wu, Xia Li, et al.were transfected with treatment and control plasmids, along with the pRL-TK plasmid (Promega, USA, E2241) serving as an internal standard following a method previously described [38]. The over-expression plasmid

(72) Histone demethylase KDM9 activates the CCND1/AKT pathway to promote the malignant progression of gliomas through interaction with BRD2Cellular and Molecular Life SciencesNovember 26, 2025Jin Cheng, Xingzhi Peng, Siyuan Tang, Peijun Zhou, Zhuan Zhou, Zenghui Mao, Dan Li, Lifang Yang, Jinwu Pengluciferase reporter vector pGL3-Basic (E1751) and renilla luciferase reporter gene control vector pRL-TK (E2241) were obtained from Promega (Madison, WI). The ApaI (1604, Takara, Tokyo, Japan) and EcoRI (1611, Takara)

(73) A luminescence-based biosensor to measure endogenous UBE3A activityiScienceNovember 21, 2025Lei Xing, Sophia U. Lamberti, Hannah C. Nourie, Trinity E. Rust, Wenxin Hu, Mark J. Zylkaluciferase-expressing construct driven by herpes simplex virus HSV-thymidine kinase (TK) promoter (pRL-TK, #E2241) were purchased from Promega. Firefly-luciferase coding sequence was amplified from BAR-pGL3 vector10

(74) UHRF1 restricts HCoV-229E infection through epigenetic silencing of the viral receptor APNNature CommunicationsNovember 18, 2025Pengcheng Wang, Ziqiao Wang, Fei Feng, Lisha Yin, Yuyuan Zhang, Zhichao Gao, Jiannan Chen, Ping Zhang, Shuiqiao Yuan, Qiang Ding, et al.unmethylated or methylated luciferase reporter plasmid pGL3-APN-Luc and 50 ng of pRL-TK plasmid (Promega # E2241). Luciferase activity was measured at 24 h post-transfection using the Dual-Luciferase Reporter Assay System

(75) OSA Initiates Histone Lactylation That Drives PDE4B / FUS / AGT Axis to Pulmonary HypertensionCell ProliferationNovember 17, 2025Li Yang, Qing Ni, Yan He, Shijie Liu, Lulu Gan, Anni Dai, Yang Hu, Qian Liu, Xueling Yang, Jiqian Li, et al.500 ng reporter plasmid (pGL4.10[luc2], Promega, E6651) and 50 ng pRL‐TK (Renilla control, Promega, E2241) using Lipofectamine 3000 (Invitrogen, L3000001). 2.18.2 Dual‐Luciferase Assay Forty‐eight hours post‐transfection,

(76) Endogenous fine-mapping and prioritization of functional regulatory elements in complex genetic lociCell GenomNovember 12, 2025Zhao K, Zhou Y, Wang X, Wang Z, Xu X, Chen Y, Zhao L, Wu C, Wang J, Yao H, et al.al.87",RRID:Addgene_48138 pGL3-basic,Promega,Cat#E1751 pGL3-promoter,Promega,Cat#E1761 pRL-TK Renilla,Promega,Cat#E2241 ,, Software and algorithms,Software and algorithms,Software and algorithms ,, GraphPad Prism,N/A,https://www.graphpad.com/features FastQTL,"Ongen,

(77) PCBP2 Regulates p16 INK4a ‐Dependent Cellular Senescence in Response to IronAging CellNovember 11, 2025Shaoyang Yan, Lili Lu, Yuwei Wu, Zewen Du, Ebin Johny, Partha Dutta, Gang LiAdditionally, we transfected the same amounts of an internal control vector, pRL‐TK (Promega, Cat#: E2241), which constitutively expressed the Renilla luciferase. Relative luciferase reporter activities were

(78) Deciphering oligomeric proanthocyanidins’ dual osteoprotective mechanisms at single-cell resolution: NR4A1-mediated PTGS2 suppression and β-catenin-Runx2 activationFrontiers in ImmunologyNovember 4, 2025Li Huang, Yuwei Sun, Yi Zheng, Shicheng Qiu, Jianping Zheng, Chunhan Sun, Mingwei Chen, Shaowei Zheng, Yirong Zengplasmid and 100 ng of pRL-TK Renilla luciferase vector (Promega Corporation, Madison, WI, USA, Cat. No. E2241) as an internal control via Lipofectamine 3000 (Thermo Fisher Scientific, Waltham, MA, USA, Cat. No.

(79) SAG1.3-derived Frizzled-targeting small-molecule compoundsJournal of Biological ChemistryNovember 1, 2025Lukas Grätz, Ainoleena Turku, Pawel Kozielewicz, Carl-Fredrik Bowin, Magdalena M. Scharf, Jan H. Voss, Julia Kinsolving, Rawan Shekhani, Nuria Oliva-Vilarnau, Tobias Koolmeister, et al.sequence for the human TK promoter (note: weak promoter sequence) was amplified from pRL-TK (Promega; E2241) and used to replace the cytomegalovirus promoter present in the original FZD5–DEP–Clamp plasmid (26)

(80) Sexual dimorphism in colon is mediated by an androgen-IL33+ stromal cell axisCell BiosciOctober 29, 2025Haoyu Wang, Baowei Jing, Juan Zou, Tian Lan, Mengxin Hu, Lan Lin, Hanhua Cheng, Rongjia Zhoueach well of a 48-well plate were transfected with 0.5 µg recombinant constructs and 10 ng pRL-TK (E2241, Promega). Luciferase activities were subsequently measured using the dual-luciferase reporter assay

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pRL-TK Vector

pRL-TK Vector from Promega

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pRL-TK Vector from Promega

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