pRL-TK Vector from Promega

Product Specs
ItempRL-TK Vector
CompanyPromega
Price $0.00Supplier Page View Company Product Page
Catalog NumberE2241
HostMammalian
PromoterHSV TK promoter
Vector NamepRL-TK
Quantity20microg
ReporterRenilla luciferase
ResistanceAmpR
Molecular Weight~73kDa
Selectable MarkerAmpicillin

Specifications/Features

Vector Size:
4045 bp

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(41) 4-(5-Chloro-3-(3,4,5-trimethoxybenzoyl)-1H-indol-1-yl)benzenesulfonamide: A Novel Polypharmacology Agent to Target Carbonic Anhydrase IX and XII With Improved Selectivity, Wnt/β-Catenin Signaling Pathway, and P-GlycoproteinChemMedChemFebruary 25, 2026Michela Puxeddu, Rosa Bordone, Claudia Colla, Gabriele Rotili, Antonio Coluccia, Pietro Sciò, Petra Cuřínová, Alessio Nocentini, Claudiu T. Supuran, Serena Filiberti, et al.FOP control plasmid (M51 Super 8x FOPFlash Addgene #12457) in combination with TK Renilla (Promega #E2241) using DreamFect Gold (OZ Biosciences #DG80500) according to the manufacturer's instructions. After

(42) Semaphorin 6D drives anti-tumor type I interferon responses to reprogram the tumor microenvironment in colorectal cancerCell Death & DiseaseFebruary 24, 2026Wei Shi, Fan Zhang, Wei-Qing Sun, Li-Qi Liang, Shamalagowri Krishnanluciferase reporter construct (pGL3-ISRE) and the Renilla luciferase normalization vector (pRL-TK; Promega #E2241) at a 10:1 ratio using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA). Firefly and Renilla luciferase

(43) Lung cancer-enriched p53 mutants occupy canonical p53 target genes without activating transcription, revealing a distinct loss-of-function behaviorbioRxivFebruary 20, 2026Mason A. Tracewell, Hailey N. Shankle, Samantha M. Barnada, Khushali S. Vyas, Kevin M. Kim, Theodhora Qyshkollari, Jonathan E. Karlin, Julie A. Barta, Steven B. McMahonvectors (WT, V157F, R158L, or EV), and 200ng of pRL-TK Renilla luciferase control reporter vector (E2241, Promega) using Lipofectamine 3000 (L3000015, Invitrogen) according to manufacturer’s instructions.

(44) Tumor-immune-neural circuit disrupts energy homeostasis in cancer cachexiaCancer CellFebruary 12, 2026Xiuhui Shi, Alex X. Arreola, Zhijun Zhou, Jingxuan Yang, Mingyang Liu, Yang Cai, Yu Ren, Hao Yuan, Qun Chen, Xinjie Chen, et al.ZFP64-ORF expression vector (#EX-Y4764-Lv207, Genecopoeia), pGL4.10-ZIP4 vector, and pRL-TK Vector (#E2241, Promega) using Lipofectamine 3000 Reagent (#L3000015; Invitrogen). At 48 h after transfection, the

(45) KRAB Zinc‐Finger Protein ZNF205 Promotes Hepatocellular Carcinoma via p53 Pathway RepressionCancer ScienceFebruary 10, 2026Xiaofen Huang, Yingchuan Yang, Yuan Ma, Wei Hao, Jingzhuo Jin, Yue Ding, Yiming Zhang, Xiuyuan Zhang, Xinli Li, Qin Song, et al.with other expression plasmids and the pRL‐TK Renilla luciferase reporter plasmid for normalization (E2241, Promega, USA), were co‐transfected into p53+/+ HCT116 cells. After 48 h, cells were lysed in Passive

(46) TFE3 fusion proteins drive TFE3 rearranged renal cell carcinoma progression via PGC-1α-mediated fatty acid oxidationFrontiers in ImmunologyFebruary 4, 2026Fan Feng, Yanhao Xu, Zhenggen Deng, Xiang Dong, Guijuan Chen, Wenliang Ma, Dongmei Li, Weidong Ganwhich contains the firefly luciferase gene, using Lipofectamine 2000. The PRL-TK plasmid (Promega, E2241) was co-transfected at a 100:1 ratio as an internal control. After 48 hours of transfection, cells were

(47) Transcriptomic landscape of transposable elements reveals LTR7 - PLAAT4 as a potential oncogene and therapeutic target in pancreatic adenocarcinomaGenome ResearchFebruary 3, 2026Meilong Shi, Chuanqi Teng, Shan Zhang, Xiaobo He, Lingyun Xu, Fengxian Han, Rongqi Wen, Ganjun Yu, Jingwen Liu, Yang Feng, et al.deliver 400 ng of the promoter-luciferase vector and 200 ng of the pRL-TK Renilla vector (Addgene, #E2241) into 3 × 104 AsPC-1 cells and 3 × 104 293T cells. Luciferase levels were measured after 24 h of incubation

(48) TGFβ1 Activates Lnc‐APUE to Promote Tumor Metastasis via the Alu Element‐Driven STAU1‐Mediated Decay of CDH1 mRNAAdvanced ScienceFebruary 3, 2026Song‐Yang Li, Jia‐Hui Huang, Jin‐E Yang, Yi‐Hang Li, Juan‐Zhen Hong, Ting‐Ting Wang, Ying‐Lei Chi, Meng‐Zhi Wu, Wei Wang, Ying Zhu, et al.P(‐505/+70), P(‐212/+70), P(delSBE1/2), P(delSBE3) and P(mutSBE1/2). For normalization, the pRL‐TK vector (E2241, Promega) expressing Renilla luciferase was used as a control. 4.4 Cell Lines Human embryonic kidney

(49) The ASFV CD2v protein inhibits apoptosis by inducing proteasomal degradation of Bim EL via activation of the TPL2-MEK-ERK signaling pathwayJournal of VirologyFebruary 3, 2026Jianyu Zeng, Xin Zhang, Jingting Zhao, Qiang Li, Zhiyong Xiang, Fengyang Shi, Hua Wang, Wenlian Weng, Qiongqiong Zhou, Peng Gao, et al.ng of ASFV structural protein plasmid, 400 ng pAP-1-Luc reporter vector, and 8 ng pRL-TK (Promega, E2241) per well using Lipofectamine 2000 for 36 h. Inhibitors or DMSO (control) were added 12 h post-transfection.

(50) Single-cell analysis identifies BASP1 as a driver of drug resistance and cell plasticity in oral squamous cell carcinomaJournal of Biological ChemistryFebruary 1, 2026Abinash Behera, Sudeshna Datta, Sibasish Mohanty, Pallavi Mohapatra, Shamima Azma Ansari, Sreeparna Podder, Rachna Rath, Dillip Kumar Muduly, Rajeeb K. Swain, Sunil K. Raghav, et al.was a gift from Randall Moon (Addgene, plasmid # 12456) (47) and the pRL-TK Renilla (Promega, Cat No# E2241) luciferase control plasmid at a 100:1 ratio using ViaFect transfection reagent. The transfection was

(51) Picornavirus VP2 protein suppresses innate immunity through selective autophagic degradation of IKBKE/IKKεAutophagyFebruary 1, 2026Kangli Li, Xiangle Zhang, Dandan Dong, Boning Zhu, Shuo Wang, Xiaodan Wen, Weijun Cao, Yi Ru, Hong Tian, Guoliang Zhu, et al.transfected with a combination of 50 ng of reporter plasmid (IFNB, ISRE), 5 ng of pRL-TK (Promega, E2241) serving as an internal control, and 50 ng of the specified plasmid. At 24 h post transfection (hpt),

(52) ZIC2 drives colorectal cancer progression by regulating QPRT-mediated cell migrationFrontiers in ImmunologyJanuary 29, 2026Liken Zheng, Hao Liu, Ailing Yangtransfected with 100 ng firefly reporter plasmid, 10 ng Renilla control plasmid (pRL-TK; Promega, Cat. No. E2241), and 500 ng pcDNA3.1 empty vector or pcDNA3.1-ZIC2 using Lipofectamine 3000 (Invitrogen, Cat. No. L3000015).

(53) Functional variants at 1p36.23 confer risk of schizophrenia through modulating RERENature CommunicationsJanuary 24, 2026Yixing Liu, Junyang Wang, Hong Yang, Yifan Li, Changgai Mu, Xinglun Dang, Xinran Chen, Danyang Li, Mingna Li, Jiewei Liu, et al.of plating, 100 ng of recombinant vector and 20 ng of the pRL-TK vector (internal control; Promega, E2241) were transfected into HEK-293T cells. For SH-SY5Y and U251 cells, 150 ng of recombinant vector and

(54) The castration-resistant prostate cancer-associated SNP rs11067228 facilitates neuroendocrine differentiation through an enhancer-mediated chromatin interaction with SRRM4International Journal of Biological SciencesJanuary 8, 2026Yuan Jiang, Zhenhao Zhao, Peng Li, Guangsong Su, Yuyang Qian, Yuting Zhao, Bo Wang, Yunlong Bai, Lei Zhang, Zhongfang Zhao, et al.plates and cultured overnight. Reporter plasmids and the pRL-TK Renilla luciferase control vector (E2241, Promega) were then co-transfected into 22Rv1 cells using Lipofectamine 3000 reagent (Invitrogen). Cells

(55) A fibroblast-like endothelial cell state promotes atherosclerosis via C/EBPβ-activated TGF-β signalingThe EMBO JournalJanuary 6, 2026Linge Fan, Yingyi Zhu, Yi Li, Zixin Ji, Kefan Ma, Ying Zhang, Leiting Wei, Junbo Chen, Yuanqing Jiang, Dongwu Lai, et al.luciferase reporter vector, 1 μg of empty vector or CEBPB overexpression vector and 0.2 μg pRL-TK (Promega, E2241). Cells were cultured for 48 h and detected using the Dual Luciferase Reporter Gene Assay Kit (YEASEN,

(56) Multidimensional mapping of stimulation-responsive regulatory elements and candidate causal variants in T cell activationSci AdvJanuary 1, 2026Zhao Wang, Wei Dong, Xiangling Feng, Xinran Xu, Yao An, Huiling Xiao, Menghan Luo, Qian Liang, Ruoxuan Bai, Ke Zhao, et al.of the empty vector) and cotransfected with 20 ng of the pRL-TK Renilla Luciferase vector (Promega, E2241) into 293T cells in 24-well plates using the Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific

(57) Drynachromoside A from Drynaria fortunei attenuates oxidative stress and fibrosis of diabetic nephropathy via activating Nrf2 in vitro and in vivoRedox ReportDecember 31, 2025Huankai Yao, Jindong Li, Ruiqing Wu, Dan Du, Fengwei Ai, Yan Li(pGL4.37[luc2P/ARE/Hygro]) (Cat. no. E3641), Renilla luciferase reporter plasmid (pRL-TK) (Cat. no. E2241) and dual-luciferase reporter assay system (Cat. no. E1910) were obtained from Promega (Madison, WI,

(58) KLF13-Mediated CES2 Upregulation via p300-Dependent Acetylation Sensitizes Gastric Cancer Cells to IrinotecaniScienceDecember 19, 2025Hai-bin Zhang, Ren-hao Hu, Ke-hui Zhang, Xi-mao Cui, Shun Zhangvector,Sigma-Aldrich,Cat#E2150 pGL4.10[luc2] vector,Promega,Cat# E6651 pRL-TK vector,Promega,Cat# E2241 KLF13-pFLAG expression plasmid,This paper,N/A "KLF13 mutant plasmids (K226R/227R, K226Q/227Q)",This

(59) Regulation of glypican 6-mediated Wnt activation maintains TDP-43 nuclear localization in neuronsScientific ReportsDecember 11, 2025Nan Zhang, Shanthini Sockanathaninformation. TOPFlash luciferase assay Renilla luciferase control construct (pRL-TK) was purchased from Promega (E2241). Dual-luciferase reporter assay was performed according to the manufacturer’s instructions (Promega,

(60) Nucleolar MED19 regulates 2'-O-methylation of rRNA in supporting cancer cell growthNucleic Acids ResDecember 11, 2025Yang Ming, Zhao Jiamin, Peng Yu, Lu Chengjiang, Zhang Yiyang, Zhang Hanqing, Qiang Zhou, Gang Wangsequence into the 5′ end of the Rennila luciferase sequence in the original pRL-TK plasmid (Promega, #E2241). All the constructs were verified by Sanger sequencing. All the oligonucleotides were synthesized by

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pRL-TK Vector

pRL-TK Vector from Promega

Description

Specifications/Features

Promega

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pRL-TK Vector from Promega

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