pRL-TK Vector from Promega

Product Specs
ItempRL-TK Vector
CompanyPromega
Price $0.00Supplier Page View Company Product Page
Catalog NumberE2241
HostMammalian
PromoterHSV TK promoter
Vector NamepRL-TK
Quantity20microg
ReporterRenilla luciferase
ResistanceAmpR
Molecular Weight~73kDa
Selectable MarkerAmpicillin

Specifications/Features

Vector Size:
4045 bp

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(21) TBX20 promotes doxorubicin resistance in breast cancer cells through enhanced ABCC1 expression and inhibition of mitophagyPLoS OneJuly 17, 2026Shuai Nie, Dejian Liu, Jun Hu, Yu Gan, Liangliang Mireagent (Invitrogen, L3000015) at a 1:3 plasmid-to-reagent ratio. A control plasmid, pRL-TK (Promega, E2241), expressing Renilla luciferase, was co-transfected at 20 ng per well to normalize for transduction

(22) The STAT3-CCND2 Axis Drives a Proliferative Metaplastic Precursor Population in Gastric Intestinal MetaplasiaJ Cell Mol MedJuly 15, 2026Fazhan Li, Huijuan Wen, Feifei Ren, Pengyuan Zheng, Simeng LiuTransfection efficiency was monitored by co‐transfection of a Renilla luciferase control plasmid (pRL‐TK, Promega, USA, E2241) [41]. Luciferase activities were quantified 48 h post‐transfection using a dual‐luciferase assay system

(23) A membrane homeostatic response to lipid overload coordinates fatty acid metabolismbioRxivJuly 6, 2026Xiaojun Xiang, Yohannes A. Ambaw, Ozlem Tok, Sheng Hui, Wei-chun Tang, Arda Mizrak, Qingyue Zhang, Luke Cohen-Abeles, Robert V. Farese, Tobias C. WaltherPPARG, OHu24140D). pGL4.29[luc2P/CRE/Hygro] and pRL-TK vectors were purchased from Promega E8471 and E2241). The luciferase reporter containing the PPRE from the human CPT1A promoter (PPRE in hCPT1A) was generated

(24) Macrophage INSIG1 deficiency drives psoriasiform dermatitis via the SREBP2-STAT1 axisNature CommunicationsJuly 2, 2026Xiangzheng Li, Yu Zang, Xi Li, Tiancheng Sheng, Shaowen Liu, Leyi Chen, Xiaohe Xu, Lin Wang, Rongzhuo Long, Mengfan Zhang, et al.pGL3 STAT1-Luc, pGL3 STAT1-Luc mutant, and pRL-TK (used as a transfection efficiency control; Promega, E2241) using Lipofectamine 3000 (Cat. #L3000075, Thermo Fisher Scientific Ltd. USA) according to the manufacturer’s

(25) Microsomal triglyceride transfer protein restricts steroid production in Leydig cells by regulating SREBP2iScienceJune 19, 2026Atrayee Chattopadhyay, Munichandra Babu Babu Tirumalasetty, Thomas Palaia, Bhargavi Gangula, Rachel Ruoff, Qing Robert Miao, M. Mahmood HussainS1 ,, Recombinant DNA,Recombinant DNA,Recombinant DNA ,, pSynSRE-T-Luc,Addgene,Cat #60444 pRL-TK,Promega,Cat# E2241 ,, Software and algorithms,Software and algorithms,Software and algorithms ,, GraphPad Prism,GraphPad

(26) IGFL2 suppresses gastric cancer cell sensitivity to docetaxel by activating the Toll-like receptor signaling pathwayiScienceJune 19, 2026Wei Huang, Zhirui Tao, Xiaolin Xu, Huangming Cao, Li Cheng, Jin LiDNA ,, pGL3-TLR4 promoter reporter plasmid,GeneChem,N/A pRL-TK Renilla luciferase plasmid,Promega,Cat# E2241 ,, Software and algorithms,Software and algorithms,Software and algorithms ,, GraphPad Prism 9,GraphPad

(27) Gemcitabine activates the Hippo signaling pathway and suppresses tumor growth by stabilizing large tumor suppressor kinase 2 through the hypoxia-inducible factor 1-alpha/ubiquitin protein ligase E3 component N-recognin 5 axisJ Cell Commun SignalJune 17, 2026Jin Zhang, Lixia Xu, Kai Guo, Weijia Dong, Xin Dai, Donghua Guco‐transfected with 400 ng of reporter plasmid, 50 ng of the Renilla luciferase internal control plasmid pRL‐TK (E2241, Promega, USA), and 400 ng of oe‐HIF1A or an equivalent amount of empty vector. After 24 h, cells were

(28) PRDM1 Drives a TIM3+ Macrophage Immunosuppressive Niche via LGALS9 Signaling in Prostate Cancer ProgressionOncology ResearchJune 16, 2026Yangyang Zhang, Ilyar Mamtili, Yonghao Chen, Guangjie Ji, Chaozhao LiangRenilla luciferase internal control plasmid used for normalization was the pRL-TK Vector (Promega, Cat# E2241, Madison, WI, USA). For each well of a 24-well plate, cells were co-transfected with 400 ng of HAVCR2

(29) Role of LDHA in senescent fibroblast exosomes promoting ferroptosis via histone lactylation-mediated ACSL4 regulation in skin photoagingEpigenetics & ChromatinJune 14, 2026Tingting Yan, Lixia Huang, Jingjing Tao, Yunling Yan, Yiping Zhong, Xuebiao PengChina). The empty pGL3-Basic vector served as the negative control, and the pRL-TK vector (Promega, E2241, USA), expressing Renilla luciferase, was used as an internal control for normalization. HaCaT cells

(30) CL-316243 facilitates stable atherosclerotic plaque phenotypes in association with suppression of perivascular adipose tissue ferroptosis via upregulating C/EBPβRedox BiolJune 13, 2026Yuanqing Jiang, Yi Li, Kefan Ma, Suxiang Guo, Nachuan Liao, Jiayi Zhou, Junbo Chen, Ruizhe Ren, Yaohui Kou, Jinying Li, et al.1 μg of GPX4 plasmid (WT or Mutant 1-5), 1 μg of empty vector or C/EBPβ and 0.2 μg pRL-TK (Promega, E2241). Cells were cultured for 48 h and detected using the Dual Luciferase Reporter Gene Assay Kit (YEASEN,

(31) Pseudorabies virus infection induces IRF1-dependent PANoptosis to provoke the excessive release of HMGB1 and IL-1βCommunications BiologyJune 5, 2026Liangzheng Yu, Yue Chen, Zhenbang Zhu, Herman W. Favoreel, Xiangdong Ligene knockdown were purchased from Addgene (Massachusetts, USA). Dual-luciferase reporter plasmids pRL-TK (E2241), vector pGL3-Basic (E1751) were purchased from Promega (Wisconsin, USA). Eukaryotic expression vector

(32) Enhancing antitumor immunity in triple-negative breast cancer through reversal of T-cell exhaustion using a CD8-targeted and membrane-camouflaged nanocarrier delivering small interfering RNA against sorting Nexin 9Journal of NanobiotechnologyJune 4, 2026Wei Hu, Qishuai Chen, Yan Ma, Yang Liu, Shusheng Qiu, Xianing Dong, Zengjun Zhu, Xuanxuan Wu, Jianxin Du, Yanbin Xu, et al.reporter plasmids, sh-NFATc2 or negative control shRNA (sh-NC), and the Renilla luciferase vector pRL-TK (E2241, Promega, USA) using Lipofectamine 2000 (11668019, Thermo Fisher Scientific, USA). After 6 h, the medium

(33) UCHL3 Regulates Subgenomic Flaviviral RNA Condensates to Promote Virus PropagationAdvanced ScienceJune 3, 2026Oscar Trejo‐Cerro, Anna Beekmayer‐Dhillon, Qi Wen Teo, Lewis Siu, Ming Yuan Li, Kate J. Heesom, Adán Pinto‐Fernández, Sumana Sanyalco‐transfected with the pISRE‐Luc reporter plasmid and the Renilla luciferase control plasmid pRL‐TK (Promega, E2241; 25 ng/well) as an internal normalization control, together with either the ZIKV sfRNA‐BoxB construct

(34) CEBPG‐Mediated Palmitic Acid Adaptation of Cancer‐Associated Fibroblasts Drives Metastasis of Oral Squamous Cell CarcinomaAdvanced ScienceJune 2, 2026Yiling Duan, Yitong Li, Yufei Wu, Xiao Yang, Rui Li, Hui Zhao, Zhengjun Shangstandard PCR‐cloning. 293T cells were co‐transfected with the luciferase construct and pRL‐TK (Promega, E2241) under conditions with or without transfection of pcDNA3.1‐CEBPG. Detection was performed using the

(35) Hesperetin-Black Phosphorus Nanosheets Targeting TAMs Induce Ferroptosis and Reverse Protumoral Polarization to Remodel the Immune MicroenvironmentMaterials Today BioMay 29, 2026Liang Feng, Huan Wang, Zuodi Liang, Ying Tian, Yang Guo, Zhuo Liwere co-transfected with these constructs (1 μg) and the pRL-TK Renilla reference plasmid (0.1 μg, E2241, Promega) employing Lipofectamine 3000 (Thermo Fisher Scientific, USA). After a 48-h incubation, cell

(36) CDK4 Mediates Cisplatin Resistance in Renal Cell Carcinoma (RCC) Cells by Regulating the ASH1L-CTR1 AxisOncology ResearchMay 21, 2026Wenjian Zeng, Xianglong Li, Hao Cai, Qingyu Zhou, Shuangshuang Sun, Pingping Li, Sunlong Li, Zhi Chenof the internal reference vector pRL-TK (containing the Renilla luciferase gene, Promega, Cat. No.: E2241, Madison, USA) to correct transfection efficiency, 2 μL of Lipofectamine 3000 transfection reagent (Thermo

(37) Equine infectious anemia virus blocks interferon responses through Rev-mediated activation of the stress granule-PKR-eIF2α pathwayPLoS PathogMay 21, 2026Huiling Ren, Kewei Chen, Bingqian Zhou, Weiguo Zhang, Xue-Feng Wang, Xiaojun Wang(Sigma-Aldrich, E7658). The pEGFP-N1 vector (HG-VYC0086) was purchased from Clontech. The pRL-TK vector (E2241) was purchased from Promega. The mutants of Rev and G3BP1 were generated using PCR-based site-directed mutagenesis

(38) APOE+ macrophages induce tumor cell metastatic characteristics via TNFSF12/TNFRSF12A signaling, correlating with poor patient prognosisCancer Cell InternationalMay 20, 2026Chuanjun Song, Hongyu Sun, Wenyuan Gan, Quanzhong Liu, Lingxiang Wu, Mengyan Zhu, Lingjun Zhucorresponding control vector, and the pRL-TK Renilla luciferase control reporter vector (Promega, Catalog #: E2241) as an internal control. Luciferase activity was measured 24–48 h after transfection using the Dual-Luciferase Reporter Assay System

(39) Linalool targets NR3C2 to inhibit NF-κB-mediated gastric cancer progressionCell DivisionMay 18, 2026Ying Hu, Shunfeng Chen, Yu Wang, Ling Liu, Chaoyi Zhang, Hong Chen, Yuanyuan Duanmu, Zhaotian Su, Jun Maassay, the NFKBIZ promoter reporter plasmid and the pRL Renilla Luciferase Control Reporter Vector (E2241, Promega) were co-transfected into each well. For the assessment of NF-κB transcriptional activity,

(40) Nuclear factor IX promotes endometriosis progression through transcriptional activation of tetraspanin-2J Mol Med (Berl)May 16, 2026Yuqi Liu, Qing Xue, Jingwen Zhu, Cheng Zeng, Xin Li, Jing Shang, Jie Li, Peili Wuluciferase reporter construct, along with 90 ng of Renilla luciferase reporter plasmid pRL-TK (Promega, E2241) as an internal control. Cells were gathered 48 h post transfection, and the luciferase assay system

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pRL-TK Vector

pRL-TK Vector from Promega

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Promega

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pRL-TK Vector from Promega

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