pRL-TK Vector from Promega

Product Specs
ItempRL-TK Vector
CompanyPromega
Price $0.00Supplier Page View Company Product Page
Catalog NumberE2241
HostMammalian
PromoterHSV TK promoter
Vector NamepRL-TK
Quantity20microg
ReporterRenilla luciferase
ResistanceAmpR
Molecular Weight~73kDa
Selectable MarkerAmpicillin

Specifications/Features

Vector Size:
4045 bp

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(21) Complement factor B deficiency promotes lung cancer progression via FOS-mediated tryptophan metabolism and immune suppressionCommunications MedicineMay 12, 2026Chenglu He, Rong Gong, Fujun Zhang, Qiuyue Xu, Ying Zhao, Caini Zhang, Yu Fu, Ya Li, Yong Duan(BPS Bioscience, Catalog #78672) and transfected with pRL-TK Renilla luciferase control plasmid (Promega, catalog #E2241). Luciferase activity was measured 24 h post-transfection using the Dual-Luciferase Reporter Assay System

(22) HDAC7-Induced Epigenetic Repression Modulates ATF3 Functional Plasticity in Colorectal Cancer PathogenesisInt J Biol SciMay 1, 2026Qi Wang, Donglei Ji, Yanjie Jia, Shuang Li, Wenjing Gao, Tingting Liang, Yingying Liang, Caroline Zeng, Chunyu Wang, Ka Lung Cheung, et al.(-1486 to +114 relative to TSS), was co-transfected with the pRL-TK normalization vector (Promega, E2241) in a 10:1 ratio using Lipofectamine 3000. Firefly and Renilla luciferase activities were measured sequentially

(23) Molecular mechanism of miR-27a-3p targeting FBXW7 regulating the malignant behavior of osteosarcoma cellsTranslational Cancer ResearchApril 30, 2026Huaibin Zhang, Rongxuan Liu, Xiaorong Liu, Wenqiang Liang, Wenji Wang, Yongping WangLipo6000TM transfection reagent. A Renilla luciferase plasmid (pRL-TK, 0.02 µg/well, Promega, USA, Cat. No. E2241) was co-transfected as an internal control. Forty-eight hours post-transfection, the luciferase activity

(24) Clec3b⁺ fibroblasts are the primary effectors of portal fibrosis following activation via a KLF4/periostin axisNature CommunicationsApril 29, 2026Lin Lei, Chenchen Zhao, Wenwen Gao, Guowen Liu, Menglin Liu, Guoyue Lv, Zhongqi Fan, Yongkang Cheng, Jinxia Li, Fanrong Kong, et al.Supplementary Table 6. All plasmids were confirmed by DNA sequencing. The pRL-TK plasmid (Promega, E2241) was obtained from Promega and included in the transfection mix as a reference control encoding Renilla

(25) Tissue-selective COPII modulator SEC16B aggravates cardiovascular disease by promoting lipid exportThe EMBO JournalApril 24, 2026Xiao Wang, Yating Hu, Lu Liu, Runze Huang, Yawei Wang, Bing Liu, Yifei Zhao, Yuangang Zhu, Jia Lv, Liang Liu, et al.2018 10.1073/pnas.1704639115" Firefly,luciferase Promega,Cat# E1751 Renilla,luciferase Promega,Cat# E2241 AAV- HCRApoE/hAAT-hPCSK9 D374Y,Addgene,Cat #58379 CMV-HA-SEC13,This paper,N/A CMV-mGFP-SEC61β,This

(26) MYC-bound enhancer RNAs in cis regulate gene transcription and tumorigenesisSci AdvApril 24, 2026Sihan Li, Zehua Wang, Phuong Nguyen, Yue Wang, Yueshan Zhao, Yifei Wang, Yuang Chen, Haozhe Huang, Xiaofei Wang, Yu Zhang, et al.transfected into MCF-7 cells using Lipofectamine 2000, and plasmid pRL-TK expressing Renilla (Promega, E2241) was used as an internal control reporter. After transfection for 24 hours, cells were subjected to

(27) Integrated drivers of basal and acute immunity in diverse human populationsCell Reports MedicineApril 21, 2026Aisha Hegab Souquette, E. Kaitlynn Allen, Christine M. Oshansky, Evadnie Rampersaud, Emily V. Walker, Li Tang, Sook-San Wong, Trushar Jeevan, Lei Shi, Stanley Pounds, et al.CO2. The following day, 1μg of pGL4.10 test plasmid and 0.01μg of pRL-TK control plasmid (Promega Cat. E2241) were added per well, according to the TransIT LT protocol. All transfections were conducted in triplicate.

(28) Multi‐Omics Insights Into the Mechanisms of Early Muscle Fiber Difference and Transformation Between Lean‐Type and Chinese Indigenous PigsAdvanced ScienceApril 17, 2026Shuailong Zheng, Hainan Wu, Min Liu, Kunpeng Wu, Shuntao Huang, Zulfiqar Ahmed, Lenox Pius, Mengjin Zhu, Dequan Xuaccurate transfection efficiency, the cells were co‐transfected with 0.04 µg of the pRL‐TK (Promega, #E2241). 5.23 CUT&Tag qPCR CUT&Tag qPCR was performed using the Hyperactive Universal CUT&Tag Assay for Illumina

(29) Extracellular signalling regulates gastrin transcription through site-specific phosphorylation and nuclear redistribution of MeninbioRxivApril 13, 2026Uloma B. Elvis-Offiah, Ziyu Wen, Xianxin Hua, Juanita L. Merchanttransfection efficiency, cells were also co-transfected with the PRL-TK Renilla Reporter Plasmid (Promega, #E2241). After 24 h, cells were serum-starved for 24 h, followed by treatment with either recombinant human

(30) Microbiota-derived secondary bile acids promote STING activation and antitumor activitybioRxivApril 12, 2026Xinglin Yang, Xing Zhang, Weichao Li, Anant Gharpure, Althea Hansel-Harris, Andreas F. Tillack, Chongyang Wu, Yan-Zhu Hsieh, Ariana Sulpizio, Kyong T. Fam, et al.including pMSCV-hygro-STING (Addgene, 102598), IFN-Beta_pGL3 (Addgene, 102597), and pRL-TK (Promega, E2241) using Lipofectamine 3000 Transfection Reagent (Invitrogen, L3000–015). After 24 h, cells were treated

(31) Secretory VSMC-derived THBS1 promotes macrophage M1 polarization and inflammation in intracranial aneurysms via the CD47/NF-κB axisFront ImmunolApril 10, 2026Jianhuang Huang, Qixiu Wang, Yangyang Lin, Jianning Chen, Caihou Linluciferase reporter plasmid (Clontech, 631744) and 50 ng/well pRL-TK Renilla luciferase plasmid (Promega, E2241) as an internal control. After 48 h of co-culture, cells were lysed and luciferase activities were measured

(32) NR3C1 Modulates Wnt Signalling to Influence the Invasiveness and Immune Features of Nonfunctioning Invasive Pituitary AdenomasJournal of Cellular and Molecular MedicineApril 1, 2026Xiaoping Wang, Jinfeng Zhang, Tao Jiang, Zhijun Yang, Yu Zhang, Pinan Liu, Yuanxiang Lin#12456) or FOPflash (Addgene, #12457) (500 ng each) and the Renilla internal control vector (Promega, E2241, pRL‐TK, 50 ng), along with sh‐NC or sh‐NR3C1 as required. Cells were lysed 48 h post‐transfection,

(33) ZXDB Drives Macrophage Inflammatory Programming in Sepsis-Induced Acute Kidney Injury by Recruiting EIF4A3 to Enhance ACACA TranslationFASEB JMarch 31, 2026Haiyang Wu, Wei Gang, Li Wang, Jing Sun, Shuangxi Li, Zhiyong Guoco‐transfected with the ACACA 5′UTR reporter construct, a control Renilla luciferase plasmid (pRL‐TK, E2241; Promega), and corresponding expression plasmids (ZXDB OE, EIF4A3 KD). Firefly luciferase activity was

(34) Oligomeric lactic acid nanoplastics induce intrauterine growth restriction in mice by disrupting GATA2-mediated placental vascular developmentPLoS BiolMarch 26, 2026Jia Lv, Mengjing Wang, Changzhi Shi, Yanwei Wang, Yihao Zhang, Chang Gao, Tian-Qi Bi, Jing Yang, Youdong Xu, Qunan Wang, et al.Cellmax, USA) and co-transfected with VEGFR2-WT, GATA2-pcDNA3.1, and the Renilla control plasmid pRL-TK (E2241, Promega, USA) using Lipofectamine 2000 (11668019, Invitrogen, USA). After 48 h, Firefly and Renilla

(35) FGF4 initiates X-chromosome inactivation via activating YY1 and prompting loss of pluripotency factorsThe EMBO JournalMarch 25, 2026Lizhu Ma, Wei Fu, Lei An, Qianying Yang, Ruiqi Hai, Guangyin Xi, Yinjuan Wang, Juan Liu, Chao Zhang, Yao Fu, et al.study,N/A pCAGGS-Yy1-S120D,This study,N/A pGL4.10-Xist-promoter,"Makhlouf et al, 2014",N/A pRL-TK,Promega,Cat #E2241 pX330.puro,"Gift from Xie lab, Tsinghua University",N/A pMK289-FKBPF36V-GFP,"Gift from Xie lab, Tsinghua

(36) ZXDB Drives Macrophage Inflammatory Programming in Sepsis‐Induced Acute Kidney Injury by Recruiting EIF4A3 to Enhance ACACA TranslationFASEB JMarch 24, 2026Haiyang Wu, Wei Gang, Li Wang, Jing Sun, Shuangxi Li, Zhiyong Guoco‐transfected with the ACACA 5′UTR reporter construct, a control Renilla luciferase plasmid (pRL‐TK, E2241; Promega), and corresponding expression plasmids (ZXDB OE, EIF4A3 KD). Firefly luciferase activity was

(37) ZBTB7A promotes malignant phenotypes in ovarian cancer through transcriptional activation of CRLF1Oncology ReportsMarch 24, 2026Xiaobai Hao, Yu Chen400 ng pGL3-CRLF1-Luc reporter construct, 100 ng pRL-TK Renilla luciferase control vector (cat. no. E2241; Promega Corporation) and 500 ng either ZBTB7A-pcDNA3.1 or empty pcDNA3.1 vector (as a control) using

(38) Oncogenic GPRIN1 sustains proliferation and mitochondrial homeostasis via dual‑layer CDK1-PI3K/Akt signalling in gallbladder cancerCell Death & DiseaseMarch 21, 2026Chang Xu, Zijun Gong, Xiaojian Ni, Lingxi Nan, Wentao Sun, Houbao Liu, Xuanming Luo, Min Lico-transfected with reporter plasmids, a Renilla luciferase internal control plasmid (pRL-TK, Promega, Cat# E2241), and GPRIN1/E2F1 expression vectors or empty vector. After 48 h, luciferase activity was measured using

(39) Tamoxifen differentially modulates endometrial hyperplasia via wild-type and mutant p53 regulation of the ALKBH5-REG1A axisFrontiers in OncologyMarch 19, 2026Rencheng Wang, Jianhua Ji, Lei Liuco-transfected with firefly luciferase constructs and Renilla luciferase plasmid (pRL-TK, Promega, E2241) as an internal control using Lipofectamine™ 3000 (Invitrogen). Twenty-four hours post-transfection,

(40) Novel quinoxaline-based survivin degraders overcome docetaxel-resistance in castration-resistant prostate cancerDrug Resistance UpdatesMarch 1, 2026Caoqinglong Huang, Xunzhen Zheng, Qingbin Cui, Robert C. Peery, Zizheng Dong, Xiaohong Li, Jing-Yuan Liu, Jian-Ting ZhangLuminometer (Berthold Technologies, Bad Wildbad, Germany). Renilla luciferase activity from pRL Renilla (#E2241, Promega, Madison, WI) was also measured using the Renilla Luciferase Assay System (#E2810, Promega)

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pRL-TK Vector

pRL-TK Vector from Promega

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Specifications/Features

Promega

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pRL-TK Vector from Promega

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