pRL-TK Vector from Promega

Product Specs
ItempRL-TK Vector
CompanyPromega
Price $0.00Supplier Page View Company Product Page
Catalog NumberE2241
HostMammalian
PromoterHSV TK promoter
Vector NamepRL-TK
Quantity20microg
ReporterRenilla luciferase
ResistanceAmpR
Molecular Weight~73kDa
Selectable MarkerAmpicillin

Specifications/Features

Vector Size:
4045 bp

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) CircARID1A promotes gastric cancer progression by targeting the miR-147a /SNAI2 signaling axisCell Adhesion & MigrationDecember 31, 2026Kai Guo, Xuemei Yan, Xing Liu, Yu Peng, Weitian Xuactivity, with firefly luciferase activity being normalized against Renilla luciferase (Promega, USA, #E2241) activity. Quantitative PCR (qPCR) Using TRIzol reagent (Invitrogen, USA, # 15,596,026), total RNA

(2) circHIPK3 promotes pro-fibrotic epithelial reprogramming via the miR-29a-3p/SPARC axis with associated enhancement of TGF-β/SMAD signalingNon-coding RNA ResearchDecember 1, 2026Ying Zhou, Yixin Huang, Jiangpo Ma, Wei Wang, Zhongkai Tong, Yong Zhou, Yu Cao, Zhaoxing Dong100 ng of CAGA-luc reporter plasmid, 10 ng of pRL-TK Renilla luciferase control plasmid (Promega, #E2241), and, where indicated, 50 ng of dominant-negative SMAD3 expression plasmid (dn-SMAD3, pcDNA3-Flag-SMAD3(3SA)).

(3) STAT5A-RNF8 signaling regulates homologous recombination repair and cisplatin responsiveness in cervical cancerNeoplasiaNovember 1, 2026Weihong Lu, Weiping Wang, Lixiang Sun, Huaqin Sun, Jin wang, Wei Jiang, Mengting LuopCDNA3.1-STAT5A or pCDNA3.1 (as control), and 50 ng of Renilla Luciferase reporter plasmid (pRL-TK, Promega, E2241). Cells were harvested 48 hours post-transfection for analysis using a dual-Luciferase assay kit (Promega,

(4) Ginsenoside Rd alleviates atherosclerosis by inhibiting adipocyte ferroptosis in perivascular adipose tissuesJournal of Ginseng ResearchNovember 1, 2026Xin Fu, Chuang Liu, Fudong Hu, Jielin Qin, Tingting Wang, Xiaoke Lico-transfected with 4× RORE-firefly luciferase reporter plasmid and pRL-TK Renilla control reporter vectors (E2241, Promega Corporation, Madison, WI, USA) using Lipofectamine 3000 Transfection Reagent (L3000001, Invitrogen),

(5) FOXP1 Promotes Spermatogonial Stem Cell Proliferation by Transcriptionally Repressing GSK3B to Activate the Wnt/β-Catenin Signaling PathwayStem Cells IntOctober 19, 2026Jin-Hua Wu, Qing Feng, Feng Lin, Jiang-Ping Xie, Pei-Yan Liu, Yan-Hua Wang, Hui-Liang Zhoupromoter vectors and sh‐FOXP1 or sh‐NC using the Lipofectamine 3000 reagent. The pRL‐TK vector (Promega, E2241, Madison, WI, USA) was cotransfected as an internal reference to normalize the luciferase activity.

(6) Loss of the Asn333 high-mannose N-glycan impairs ACP3 folding and drives disease-associated dysfunctioniScienceOctober 16, 2026Noriyoshi Manabe, Kazuyoshi Itoh, Shiho Ohno, Ayumi Miura, Aika Miyata, Sora Yashima, Sachiko Komatsu, Tsutomu Fujimura, Hisatoshi Hanamatsu, Jun-Ichi Furukawa, et al.recombinant proteins" ,, Vector pcDNA3.4-TOPO,Thermo Fisher Scientific,A14697 pRL-TK plasmid,Promega Corp.,E2241 M-PER Mammalian Protein Extraction,Thermo Fisher Scientific,78501 Immobilon Forte Western HRP Substrate,Merck KGaA

(7) Apoptotic cell overload drives multinucleated giant cell formation after neoadjuvant chemotherapy in colorectal cancer: molecular mechanisms and tumor-suppressive effectsCell Death DisOctober 5, 2026Jiaxin Xue, Zhiwei Wang, Xiaoliang Zhang, Sen Xu, Shuang Wu, Xiaoyue Sun, Meiqi Li, Xueyan Wu, Zhangming Chen, Ming Ludifferent promoter regions (pGL3-Basic, pGL3-1000, pGL3-500, and pGL3-Mut) and the pRL-TK vector (#E2241, Promega, USA) using PEI 40 K (#G1802, Servicebio, Wuhan, China). After 48 h, luciferase activities

(8) Estrogen Receptor Beta Isoforms Interplay Integrates Nuclear and Extra‐Nuclear Signaling to Modulate Human Granulosa Cell GrowthFASEB JOctober 3, 2026Clémentine Marie, Inza‐Noor Baskaran, Raphaël Corre, Anne Mayeur, Michaël Grynberg, Guillaume Chevreux, David L'Hôte, Joëlle Cohen‐Tannoudji, Stéphanie Chauvinplasmid pRL‐TK, carrying the Renilla luciferase gene under the control of the TK promoter (Promega E2241) was co‐transfected to correct for variations in transfection efficiency. The reporter (ERE‐ or AP‐1

(9) ITGB2 promotes HIF-1α/GLUT1 mediated glucose metabolic reprogramming in renal tubular epithelial cells to aggravate the progression of diabetic kidney diseaseCytotechnologyOctober 1, 2026Jun Peng, Qiaomeng Yuan, Lu Zhou, Wenqi Zhao, Mingrui Zhaoco-transfected with the reporter plasmid, the Renilla luciferase internal-control vector pRL-TK (Promega, E2241), and an HIF1A expression plasmid or empty vector using Lipofectamine 3000. After 48 h, firefly and

(10) TNFα-dependent modulation of WT1-MMP9 regulatory axis links developmental and inflammatory pathways in glaucomaiScienceSeptember 18, 2026Tahseen Ahmed, Jyotishman Sarma, Meha Kabra, Shantanu Saha Roy, Samir Bera, Arun Kumar Mishra, Diganta Roy, Sayani Bagchi, Goutham Pyatla, Ashish Mishra, et al.expression plasmid,OriGene,Cat #RC220079 pGL3-Basic vector,Promega,212936 pRL-TK Renilla plasmid,Promega,E2241 ,, Oligonucleotides,Oligonucleotides,Oligonucleotides ,, wt1 morpholino,Gene Tools,Sequence in Table

(11) Demethylase FTO attenuates atherosclerotic plaque via upregulating ABCA1 and ABCG1 expression by targeting ATG5Mol Med RepSeptember 16, 2026Fengxia Guo, Mei He, Yaonan Li, Kangning Zhu, Bing Hufirefly luciferase reporter, and the pRL-TK plasmid, serving as the Renilla luciferase control vector (E2241; Promega Corporation). Additionally, shRNAs targeting either FTO (5′-GGACTTAAGGAATCCAGAATT-3′) or YTHDF1

(12) A retinoic acid-Rfx6 cascade modulates Wnt/Bmp-dependent gut tube patterning: insight into the molecular basis of Mitchell–Riley SyndromeDevelopmentSeptember 15, 2026Scott A. Rankin, Jacek Biesiada, Kyle W. McCracken, Riya K. Patel, Mehek Rana, David M. Luedeke, Daniel O. Kechele, James M. Wells, Aaron M. Zornversions of the enhancer:firefly luciferase reporters +15 pg pRL-TK Renilla luciferase plasmid (Promega, E2241) into embryos at the 16/32-cell stage into the D1 vegetal region to target the foregut or into the D4

(13) Liposomal Doxorubicin Induces PD-L1-High Tumor-Associated Macrophages and Sensitizes Triple-Negative Breast Cancer to PD-L1 BlockadeOncol ResSeptember 14, 2026Yu Zheng, Peng Zheng, Yidan Zheng, Zihan Xi, Tao HuangBiotechnology Co., Ltd. (Beijing, China). The pRL-TK Renilla luciferase control plasmid (Promega Corporation, E2241, Madison, WI, USA) was used as an internal control. BMDMs cultured in 24-well plates were co-transfected

(14) DNA Polymerase θ Drives Colorectal Cancer Progression through Wnt/β-Catenin Activation and Shows Potential Association with Immunosuppressive Microenvironment RemodelingOncology ResearchSeptember 14, 2026Li Li, Jianhua Wang, Feilong Zhou, Lingjun Geng, Kongwang HuUSA). The pRL-TK Renilla luciferase internal control plasmid was purchased from Promega Corporation (E2241, Madison, WI, USA). Stable POLQ-knockdown (shRNA) and stable POLQ-overexpressing SW480 and HCT116 cell

(15) Honokiol Suppresses Stemness and Sensitizes Triple-Negative Breast Cancer to Chemotherapy via YAP/TAZ-TEAD InhibitionOncology ResearchSeptember 14, 2026Jiang-Nan Xia, Shan-Dong Zhu, Wei-Ling Qu, Yi-Lin Hu, Wen-Yi Ma, Wenyan Wang, Qian-Lan Huang, Bing-Yuan Lin, Jia-En Guo, Ying-Wei Licontains a synthetic promoter responsive to YAP/TAZ, and 10 ng of the pRL-TK Renilla vector (Promega, #E2241, Madison, WI, USA). After a 24-h recovery period, the transfected cells were treated with honokiol (10,

(16) Targeting LOXL4-driven matrix stiffening with acetyldigoxin restores CD8+ T cell function in lung cancerSci AdvSeptember 11, 2026Xuyu Gu, Yifei Zhu, Li Xu, Xinnan Xu, Kaiqi Jin, William C. Cho, Qiyu Fangreporter, 250 ng of YAP1 or YBX1 expression plasmid, and 50 ng of Renilla control (pRL-TK; Promega, #E2241) using Lipofectamine 3000. Control groups received equivalent empty vector (pcDNA3.1). Transfection

(17) O Agonismo do GPR84 Atenua a Inflamação Endotelial Induzida por Ácidos Graxos Livres e a Adesão de Monócitos através da Via Gfi1/NF-ΚbArq Bras CardiolSeptember 10, 2026Li Li, Gang Fu, Yanling Liu, Caiyun LiuNF-κB (pNF-κB-Luc, Promega, Cat. nº E8491) e o vetor controle de luciferase de Renilla (pRL-TK, Promega E2241) utilizando Lipofectamine 3000 (Invitrogen L3000015), seguindo as especificações do fabricante. Após

(18) NAT10 is critical to block RNA sensing-induced IFN-β transactivation in viral infectionmBioSeptember 9, 2026Youngmin Park, Yan Liu, Jinshan He, Suha Eleya, Zhenyu Wu, Guillaume Nicolas Fiches, Dawei Zhou, Emily G. Watters, Zhixian M. He, Kateepe Arachchige Sudeera Nuwan Shanaka, et al.provided by Dr. Jacob S. Yount, Ohio State University) and pRL-TK renilla luciferase vector (Cat. #E2241, Promega) along with pTRIP-SFFV-mtagBFP-2A RIGI WT (Cat. #167289, Addgene) or pEBG-RIG-I 2CARD-GST (kindly

(19) A Truncated Serpin D1 Variant Regulates Adseverin-Dependent Actin Severing and Is Required for OsteoclastogenesisCellsSeptember 3, 2026Yongqiang Wang, Chenfan Ji, Andrew Y. Wang, Masoud Norouzi, Isabel Ding, Michael Glogauer, Mark Abovsky, Igor Jurisica, Christopher A. McCulloch(passage 6, 1.5 × 106) with 150 ng Renilla internal control plasmid construct pRL-TK (Promega, Cat. No. E2241 (20:1), by using an Amaxa nucleofector (Walkersville, MD, USA, program D-032) with buffer 1M reagent

(20) Novel TBX20 Variations Susceptible to Sporadic Atrial FibrillationBiomedicinesSeptember 3, 2026Zhen-Yu Xu, Dao-Liang Zhang, Xing-Biao Qiu, Chen-Xi Yang, Ying-Jia Xu, Yi-Qing Yang, Ning Liof the KCNH2-luc plasmid and 0.04 μg of the pRL-TK plasmid expressing renilla luciferase (Cat. No. E2241; Promega, Madison, WI, USA). As described previously [83,88], the HeLa and COS7 cells were collected

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pRL-TK Vector

pRL-TK Vector from Promega

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Specifications/Features

Promega

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pRL-TK Vector from Promega

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