pRL-TK Vector from Promega

Product Specs
ItempRL-TK Vector
CompanyPromega
Price $0.00Supplier Page View Company Product Page
Catalog NumberE2241
HostMammalian
PromoterHSV TK promoter
Vector NamepRL-TK
Quantity20microg
ReporterRenilla luciferase
ResistanceAmpR
Molecular Weight~73kDa
Selectable MarkerAmpicillin

Specifications/Features

Vector Size:
4045 bp

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) FOXO6 inhibits SMURF2-mediated ubiquitination and degradation of NR4A1 to promote retinal pigment epithelial cell ferroptosis in diabetic retinopathyJ Cell Commun SignalAugust 26, 2026Qiao Chen, Bo Su, Ke Xu, Liang Li, Changzheng Chenco‐transfected with 0.8 μg of pGL3‐WT or pGL3‐Mut, 0.02 μg of pRL‐TK Renilla luciferase plasmid (Cat# E2241, Promega) as an internal control, and either sh‐NC or sh‐FOXO6 plasmids using Lipofectamine 3000. After

(2) Dysregulation of the KLF9/TXNRD2 Axis Leads to Mitochondrial DNA Oxidation and NLRP3 Inflammasome Activation in Ulcerative ColitisJ Biochem Mol ToxicolAugust 24, 2026Yijia Fan, Lingling Dai, Feng Zhu, Mingfang Ping, Xiaofeng Zhu, Junguo Chenconfluence, co‐transfected with 200 ng of pGL3‐TXNRD2 promoter reporter, 50 ng of pRL‐TK internal control (E2241, Promega), and 200 ng of thKLF9 overexpression vector (pcDNA3.1‐KLF9) or an empty vector (pcDNA3.1)

(3) FHL3 Regulates Vascular Smooth Muscle Cell Phenotypic Switching Through the MRTFB-SRF Signaling AxisCellsAugust 17, 2026Xiaoxin Huang, Heming Zhang, Yanhong Zhang, Charles U. Solomon, David G. McVey, Shu Yepreviously described [29]. Each of these constructs, together with the pRL-TK control plasmid (Promega, E2241), were transfected into HASMCs, and luciferase activity was measured 2 days post-transfection using

(4) SOX4 facilitates osteogenic differentiation through suppressing NLRP3 transcription-mediated pyroptosisCytotechnologyAugust 8, 2026Ping Li, Xue-Yi Lin, Yu Lin, Yan Liu, Wei-Bing YangpGL3-mut-P2 plasmid, 0.2 µg of pcDNA3.1-SOX4 or empty vector, and 0.05 µg of pRL-TK vector (Promega, Cat. No. E2241, USA) as an internal control, using Lipofectamine 3000 reagent. After 48 h, luciferase activity was

(5) Loss of SETD2-mediated H3K36me3 Drives CD8+ T Cell Exhaustion in Lung Cancer through CXCL-dependent PMN-MDSC Recruitment and Arginine DeprivationResearch (Wash D C)August 5, 2026Xuyu Gu, Junjie Zhu, Tao Ge, Keyi Chen, Yang Yang, Kaiqi Jin, Xinnan Xuconfluence). Cells were cotransfected with 1 μg of promoter–reporter plasmid and 50 ng of pRL-TK (#E2241, Promega) as internal control. Medium was replaced 6 h later, followed by another 24 h. Cells were lysed

(6) Loss of NCCRP1 overcomes immune evasion in lung adenocarcinomaJ Immunother CancerAugust 5, 2026Lu Liu, Shihao Qi, Liye Shao, Junyang Cai, Qingqing Zeng, Xu Jiang, Fang Ma, Weiwei Lin(China). SgCtrl or sgNccrp1 LLC cells were co-transfected with each reporter plasmid and the pRL-TK (E2241, Promega) plasmid using the liposomal transfection reagent GTR. After 48 hours of transfection, firefly

(7) Transcription Factor MAX Regulates Liver Cancer Cell Growth, Migration, Invasion, and Epithelial–Mesenchymal Transition by Promoting SF3A3 ExpressionWorld Journal of OncologyAugust 1, 2026Gui Zhou, Mei Yu Dai, Xiang Chen, Li Ming Liu, Fa Quan Linhairpin RNA (shRNA) targeting MAX (100 ng), and the Renilla luciferase control vector pRL-TK (10 ng, Cat# E2241, Promega) using Lipofectamine 3000 (Thermo Fisher). After 48 h, the cells were lysed and luciferase

(8) TBX20 promotes doxorubicin resistance in breast cancer cells through enhanced ABCC1 expression and inhibition of mitophagyPLoS OneJuly 17, 2026Shuai Nie, Dejian Liu, Jun Hu, Yu Gan, Liangliang Mireagent (Invitrogen, L3000015) at a 1:3 plasmid-to-reagent ratio. A control plasmid, pRL-TK (Promega, E2241), expressing Renilla luciferase, was co-transfected at 20 ng per well to normalize for transduction

(9) The STAT3-CCND2 Axis Drives a Proliferative Metaplastic Precursor Population in Gastric Intestinal MetaplasiaJ Cell Mol MedJuly 15, 2026Fazhan Li, Huijuan Wen, Feifei Ren, Pengyuan Zheng, Simeng LiuTransfection efficiency was monitored by co‐transfection of a Renilla luciferase control plasmid (pRL‐TK, Promega, USA, E2241) [41]. Luciferase activities were quantified 48 h post‐transfection using a dual‐luciferase assay system

(10) A membrane homeostatic response to lipid overload coordinates fatty acid metabolismbioRxivJuly 6, 2026Xiaojun Xiang, Yohannes A. Ambaw, Ozlem Tok, Sheng Hui, Wei-chun Tang, Arda Mizrak, Qingyue Zhang, Luke Cohen-Abeles, Robert V. Farese, Tobias C. WaltherPPARG, OHu24140D). pGL4.29[luc2P/CRE/Hygro] and pRL-TK vectors were purchased from Promega E8471 and E2241). The luciferase reporter containing the PPRE from the human CPT1A promoter (PPRE in hCPT1A) was generated

(11) Macrophage INSIG1 deficiency drives psoriasiform dermatitis via the SREBP2-STAT1 axisNature CommunicationsJuly 2, 2026Xiangzheng Li, Yu Zang, Xi Li, Tiancheng Sheng, Shaowen Liu, Leyi Chen, Xiaohe Xu, Lin Wang, Rongzhuo Long, Mengfan Zhang, et al.pGL3 STAT1-Luc, pGL3 STAT1-Luc mutant, and pRL-TK (used as a transfection efficiency control; Promega, E2241) using Lipofectamine 3000 (Cat. #L3000075, Thermo Fisher Scientific Ltd. USA) according to the manufacturer’s

(12) IGFL2 suppresses gastric cancer cell sensitivity to docetaxel by activating the Toll-like receptor signaling pathwayiScienceJune 19, 2026Wei Huang, Zhirui Tao, Xiaolin Xu, Huangming Cao, Li Cheng, Jin LiDNA ,, pGL3-TLR4 promoter reporter plasmid,GeneChem,N/A pRL-TK Renilla luciferase plasmid,Promega,Cat# E2241 ,, Software and algorithms,Software and algorithms,Software and algorithms ,, GraphPad Prism 9,GraphPad

(13) Microsomal triglyceride transfer protein restricts steroid production in Leydig cells by regulating SREBP2iScienceJune 19, 2026Atrayee Chattopadhyay, Munichandra Babu Babu Tirumalasetty, Thomas Palaia, Bhargavi Gangula, Rachel Ruoff, Qing Robert Miao, M. Mahmood HussainS1 ,, Recombinant DNA,Recombinant DNA,Recombinant DNA ,, pSynSRE-T-Luc,Addgene,Cat #60444 pRL-TK,Promega,Cat# E2241 ,, Software and algorithms,Software and algorithms,Software and algorithms ,, GraphPad Prism,GraphPad

(14) Gemcitabine activates the Hippo signaling pathway and suppresses tumor growth by stabilizing large tumor suppressor kinase 2 through the hypoxia-inducible factor 1-alpha/ubiquitin protein ligase E3 component N-recognin 5 axisJ Cell Commun SignalJune 17, 2026Jin Zhang, Lixia Xu, Kai Guo, Weijia Dong, Xin Dai, Donghua Guco‐transfected with 400 ng of reporter plasmid, 50 ng of the Renilla luciferase internal control plasmid pRL‐TK (E2241, Promega, USA), and 400 ng of oe‐HIF1A or an equivalent amount of empty vector. After 24 h, cells were

(15) PRDM1 Drives a TIM3+ Macrophage Immunosuppressive Niche via LGALS9 Signaling in Prostate Cancer ProgressionOncology ResearchJune 16, 2026Yangyang Zhang, Ilyar Mamtili, Yonghao Chen, Guangjie Ji, Chaozhao LiangRenilla luciferase internal control plasmid used for normalization was the pRL-TK Vector (Promega, Cat# E2241, Madison, WI, USA). For each well of a 24-well plate, cells were co-transfected with 400 ng of HAVCR2

(16) Role of LDHA in senescent fibroblast exosomes promoting ferroptosis via histone lactylation-mediated ACSL4 regulation in skin photoagingEpigenetics & ChromatinJune 14, 2026Tingting Yan, Lixia Huang, Jingjing Tao, Yunling Yan, Yiping Zhong, Xuebiao PengChina). The empty pGL3-Basic vector served as the negative control, and the pRL-TK vector (Promega, E2241, USA), expressing Renilla luciferase, was used as an internal control for normalization. HaCaT cells

(17) CL-316243 facilitates stable atherosclerotic plaque phenotypes in association with suppression of perivascular adipose tissue ferroptosis via upregulating C/EBPβRedox BiolJune 13, 2026Yuanqing Jiang, Yi Li, Kefan Ma, Suxiang Guo, Nachuan Liao, Jiayi Zhou, Junbo Chen, Ruizhe Ren, Yaohui Kou, Jinying Li, et al.1 μg of GPX4 plasmid (WT or Mutant 1-5), 1 μg of empty vector or C/EBPβ and 0.2 μg pRL-TK (Promega, E2241). Cells were cultured for 48 h and detected using the Dual Luciferase Reporter Gene Assay Kit (YEASEN,

(18) Enhancing antitumor immunity in triple-negative breast cancer through reversal of T-cell exhaustion using a CD8-targeted and membrane-camouflaged nanocarrier delivering small interfering RNA against sorting Nexin 9Journal of NanobiotechnologyJune 4, 2026Wei Hu, Qishuai Chen, Yan Ma, Yang Liu, Shusheng Qiu, Xianing Dong, Zengjun Zhu, Xuanxuan Wu, Jianxin Du, Yanbin Xu, et al.reporter plasmids, sh-NFATc2 or negative control shRNA (sh-NC), and the Renilla luciferase vector pRL-TK (E2241, Promega, USA) using Lipofectamine 2000 (11668019, Thermo Fisher Scientific, USA). After 6 h, the medium

(19) UCHL3 Regulates Subgenomic Flaviviral RNA Condensates to Promote Virus PropagationAdvanced ScienceJune 3, 2026Oscar Trejo‐Cerro, Anna Beekmayer‐Dhillon, Qi Wen Teo, Lewis Siu, Ming Yuan Li, Kate J. Heesom, Adán Pinto‐Fernández, Sumana Sanyalco‐transfected with the pISRE‐Luc reporter plasmid and the Renilla luciferase control plasmid pRL‐TK (Promega, E2241; 25 ng/well) as an internal normalization control, together with either the ZIKV sfRNA‐BoxB construct

(20) CEBPG‐Mediated Palmitic Acid Adaptation of Cancer‐Associated Fibroblasts Drives Metastasis of Oral Squamous Cell CarcinomaAdvanced ScienceJune 2, 2026Yiling Duan, Yitong Li, Yufei Wu, Xiao Yang, Rui Li, Hui Zhao, Zhengjun Shangstandard PCR‐cloning. 293T cells were co‐transfected with the luciferase construct and pRL‐TK (Promega, E2241) under conditions with or without transfection of pcDNA3.1‐CEBPG. Detection was performed using the

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pRL-TK Vector

pRL-TK Vector from Promega

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Promega

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pRL-TK Vector from Promega

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