pRL-CMV Vector from Promega

Product Specs
ItempRL-CMV Vector
CompanyPromega
Price $77.00Supplier Page View Company Product Page
Catalog NumberE2261
HostMammalian
Vector NamepRL-CMV
ResistanceAmpR
ReporterRenilla luciferase
Quantity20microg
PromoterCMV Enhancer and Immediate early promoter
Molecular Weight~66kDa
Selectable MarkerAmpicillin

Specifications/Features

Vector Size:
4079 bp

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) SREBP-1 upregulates SOAT1 to promote tumor growth by preventing lipotoxicityCell ReportsFebruary 24, 2026Yaogang Zhong, Logan Mazik, Huali Su, Cheng-Yao Chiang, Feng Geng, Xiaokui Mo, Qiang Wang, Yongjun Kou, Wei Chen, Zaibo Li, et al.Addgene_12259 psPAX2,Addgene,RRID: Addgene_12260 pRL Renilla Luciferase Control Reporter Vectors,Promega,Cat# E2261 Software and algorithms,Software and algorithms,Software and algorithms GraphPad Prism Ver. 9.41,GraphPad

(2) STAT1 accelerates cutaneous melanoma progression through TUBB4A expression regulationMolecular Medicine ReportsFebruary 13, 2026Rongxin Zhao, Kexin Fang, Xiaofei Zhang, Hongchao Lico-transfected with the reporter constructs, the internal control Renilla luciferase vector (cat. no. E2261; Promega Corporation), and si-STAT1 (cat. no. A09009; GenePharma, Shanghai) as aforementioned. STAT1

(3) CD73 restrains mutant β-catenin oncogenic activity in endometrial carcinomasJCI InsightJanuary 23, 2026Rebecca M. Hirsch, Gaith Droby, Sunthoshini Premsankar, Molly L. Parrish, Katherine C. Kurnit, Lilly F. Chiou, Emily M. Rabjohns, Hannah N. Lee, Russell R. Broaddus, Cyrus Vaziri, et al.108101) (114) and pACCMV (generated in-house) served as empty vector controls. CMV-pRenilla-LUC (Promega E2261) was used as a control reporter. Plasmids were propagated in E. coli DH5α and purified with QIAGEN or

(4) Osthole Activates FGF21 Expression by Mediating Activation of ATF4 in Human Hepatocyte HepG2 CellsInternational Journal of Molecular SciencesJanuary 19, 2026Akishi Taguchi, Masaya Araki, Tomoya Yamashita, Ryo Kanazawa, Itsuki Terao, Kyohei Suzuki, Yuhei Tsuchimoto, Takashi Matsuzaka, Hirohito Sone, Hitoshi Shimano, et al.Luciferase Analysis HepG2 cells were transfected with the indicated luciferase vector and pRL-CMV (Promega, E2261, Madison, WI, USA), as a reference, using FuGENE6 (Promega, E2691, Madison, WI, USA). After 24 h of

(5) EGR Proteins Mediate Interferon‐Independent Anti‐HSV‐1 Responses Through Viral and Host TargetsAdvanced ScienceJanuary 4, 2026Shuaishuai Wang, Fujun Hou, Xunuo Jin, Yuhang Xiang, Dongli Pansynthesized by TsingKeBio, between KpnI and HindIII sites of the pGL3 vector. pRL‐CMV was from Promega (#E2261). Primer sequences used for amplification are provided in Table S3. 4.4 Chemical Inhibitors The following

(6) Hepatic metabolic reprogramming in male mice during short-term caloric restriction involves enhanced glucocorticoid rhythmsNat CommunDecember 11, 2025Konstantinos Makris, Vlera Fonda, Fania Feby Ramadhani, Lina Fadel, Morgane Davezac, Bertrand Payet, Ioannis K. Deligiannis, Liwei Zhang, Teresa Horn, Laura Heimerl, et al.reporters along with expression vectors for mouse GR and constitutively active FOXO1. pRL-CMV (Promega, #E2261) Renilla was used for normalization. On the next day, cells were treated for 24 h with 1 µM dexamethasone. Dual-Glo Luciferase Assay System

(7) Targeting a distinct binding pocket in the pregnane X receptor with natural agonist TRLW-2 ameliorates murine ulcerative colitisFront PharmacolDecember 11, 2025Shangrui Rao, Yi Mei, Lingyan Shi, Hongzheng Li, Minyu Zhou, Ziyu Meng, Zhijie Zhao, Shaotang Li, Weijian Sun, Qiang Tianand a HiScript III RT SuperMix kit (R323-01, Vazyme, China) were purchased. XREM-luc (E4121) and pRL (E2261) plasmids were purchased from Promega. The cDNA sequence of PXR was cloned into the pLenti6/v5 lentiviral

(8) tRF-21LeuTAA Promotes Oxidative Stress by Altering Glutathione Metabolic Enzymes to Support Prostate Cancer ProgressionCancer ResearchDecember 1, 2025Xiaoyu Guo, Jinjing Zhong, Jingyu Qian, Xiuyi Pan, Xinyue Zhang, Xueqin Chen, Hao Zeng, Ni Chen, Ling Nie, Ting Yu, et al.(RRID:Addgene_172006), and Renilla pRL-CMV (RRID:Addgene_41046) were purchased from Promega (TM033, E2261). LAP3 WT or Mut promoter sequences (−1,376 to +299) were prepared with the primers (WT-FP1-KpnI, WT-RP2-XhoI,

(9) Interferon alpha-inducible protein 27 (IFI27) inhibits hepatitis B virus (HBV) transcription through downregulating cellular transcription factor C/EBPαJournal of VirologyNovember 25, 2025Xiaoyang Yu, Cheng-Der Liu, Sheng Shen, Elena S. Kim, Zhentao Liu, Hu Zhang, Ning Sun, Yuanjie Liu, Pia M. Martensen, Yufei Huang, et al.100). The CMV-IE promoter Renilla luciferase reporter plasmid pRL-CMV was purchased from Promega (Cat# E2261). Plasmid FLAG-IFI27 expresses the N-terminally FLAG-tagged IFI27 (26, 101). The human IL-8 expression

(10) HBV p22-interacting protein C1QBP inhibits viral replication through impeding nucleocapsid formation and nuclear importPLoS PathogOctober 17, 2025Xiao Peng, Cheng-Der Liu, Bidisha Mitra, Elena S. Kim, Ning Sun, Andrea Jurado, Hu Zhang, Shitao Li, Tongqing Zhou, Haitao Guopreviously [98]. The CMV-IE promoter Renilla luciferase reporter plasmid pRL-CMV was purchased from Promega (E2261). Plasmid transfection was performed with Lipofectamine 3000 transfection reagent (Invitrogen, L3000150).

(11) Portal vein-enriched metabolites as intermediate regulators of the gut microbiome in insulin resistanceCell MetabolismOctober 7, 2025Vitor Rosetto Muñoz, Francois Moreau, Marion Soto, Yoshiyuki Watanabe, Loc-Duyen Pham, Jimmy Zhong, Sam Zimmerman, Bruna B. Brandao, Khyati Girdhar, Julian Avila, et al.elements,Addgene,90371 LXR response elements,Addgene,90348 pRL Renilla Luciferase Control Reporter Vector,Promega,E2261 Software and algorithms,Software and algorithms,Software and algorithms GraphPad Prism v8,GraphPad

(12) Targeted citrullination enables p53 binding to non-canonical sitesMolecular CellOctober 2, 2025Alexandra Indeglia, Andrea Valdespino, Giulia Pantella, Sarah Offley, Connor Hill, Maya Foster, Kaitlyn Casey, Hsin-Yao Tang, Anneliese M. Faustino, Alessandro Gardini, et al.tet-PADI4 KO (human),This paper (generated by Genscript),NA pRL-CMV vector (Renilla),Promega,Cat# E2261 Recombinant mononucleosomes,Active Motif,Cat# 81071 Recombinant p53,Active Motif,Cat# 81091 Recombinant

(13) Bmal1 deletion alters mitochondrial microstructure and function in mouse cone photoreceptorsiScienceSeptember 19, 2025Nicolas Diaz, Sondip Biswas, Wei Zhong, Khaleel Bashir, Ting Chung Suen, Jason DeBruyne, Paul Michael Iuvone, Gianluca Tosini, Hao Duong, Sharon Francis, et al.al.,https://doi.org/10.1016/j.freeradbiomed.2020.06.039 pCMV10/3xflag-Clock,Addgene,#47334;RRID:Addgene_47334 pcDNA™3.1,Invitrogen™,V79020 pCAGGS-FLAG-BMAL1,Addgene,#186829;RRID:Addgene_186829 pRL-CMV Renilla Luciferase,Promega,E2261 Mic-60 WT promoter-Luciferase,This paper, Mic-60 mutated E-box promoter-Luciferase,This paper, ,, Software

(14) p53 isoforms have a high aggregation propensity, interact with chaperones and lack binding to p53 interaction partnerseLifeSeptember 10, 2025Anamari Brdar, Christian Osterburg, Philipp Münick, Anne Christin Machel, Rajeshwari Rathore, Susanne Osterburg, Büşra Yüksel, Birgit Schäfer, Kristina Desch, Julian D Langer, et al.V79020) Recombinant DNA reagent,pGL3-Basic vector,Promega,, Recombinant DNA reagent,pRL-CMV,Promega,E2261, Recombinant DNA reagent,pBV-Luc BDS-2 3x WT,Addgene,Plasmid #16515, Recombinant DNA reagent,pGL2 basic vector

(15) The BAG3-HSP70-CHIP axis controls the degradation of TGFBR2 in cardiac fibroblastsJournal of Molecular and Cellular CardiologyAugust 1, 2025Margaretha A.J. Morsink, Josephine M. Watkins, Katelyn Zhu, Xiaokan Zhang, Lori J. Luo, Barry M. Fine, Bryan Z. Wang, Gordana Vunjak-Novakovic(v/w). 2.4 Luciferase assay Cells were transfected with SBE4-Luc (Addgene #16495) and pRL (Promega E2261) at a 1:1 ratio using Viafect as described. 24 hours after transfection, media was refreshed with or

(16) ALS-associated RNA-binding proteins promote UNC13A transcription through REST downregulationThe EMBO JournalJuly 24, 2025Yasuaki Watanabe, Naoki Suzuki, Tadashi Nakagawa, Masaki Hosogane, Tetsuya Akiyama, Naotoshi Kageyama, Yukino Funayama, Hitoshi Warita, Satoru Morimoto, Hideyuki Okano, et al.pGL4.12-UNC13A-WT,This study,N/A Plasmid: pGL4.12-UNC13A-ΔR,This study,N/A Plasmid: pRL-CMV,Promega,E2261 Plasmid: pSpCas9(BB)-2A-Puro(PX459) V2.0,"Ran et al, 2013",Addgene 62988 Antibodies,Antibodies,Antibodies Rabbit

(17) Hydrogel-embedded precision-cut lung slices support ex vivo culture of in vivo-induced premalignant lung lesionsPhysiol RepJuly 12, 2025Caroline Hauer, Rachel Blomberg, Kayla Sompel, Chelsea M. Magin, Meredith A. Tennisluciferase (Addgene plasmid #1015; gift from Bruce Speigelman) and 5 ng renilla control reporter vector (#E2261, Promega), mock luciferase, and empty vector. 24 h after transfection, 15 μL of media was collected

(18) TFAP2C Drives Cisplatin Resistance in Bladder Cancer by Upregulating YAP and Activating β-Catenin SignalingJournal of Biological ChemistryJune 18, 2025Qiufeng Pan, Changmin Zou, Zhigen Lin, Hao Tang, Zepu Long, Longwang Wangeither an empty vector or a TFAP2C expression plasmid, along with a Renilla luciferase control plasmid (#E2261, Promega) for normalization. After 48 h, cells were harvested and lysed, and luciferase activity was

(19) CLU alleviates Alzheimer’s disease-relevant processes by modulating astrocyte reactivity and microglia-dependent synaptic densityNeuronJune 18, 2025Alexandra M. Lish, Elyssa F.L. Grogan, Courtney R. Benoit, Richard V. Pearse, Sarah E. Heuer, Tain Luquez, Gwendolyn A. Orme, Paige C. Galle, Giedre Milinkeviciute, Kim N. Green, et al.pGL4.32[luc2P/NF-κB-RE/Hygro], encoding Firefly luciferase under a minimal promoter with an NfκB response element (Promega, #E2261 and #E849A, respectively). Day 18 iAs were transfected with the two reporter plasmids using the Amaxa

(20) A niche driven mechanism determines response and a mutation-independent therapeutic approach for myeloid malignanciesCancer CellJune 9, 2025Ioanna Mosialou, Abdullah M. Ali, Rossella Labella, Brygida Bisikirska, Alvaro Cuesta-Dominguez, Paraskevi Vgenopoulou, Ismarc Reyes, Sanjana M. Rao, Anqi Wang, Na Luo, et al.serum,Jackson ImmunoResearch,017-000-121 Lipofectamine 3000,Invitrogen,L3000015 pRL-CMV Renilla,Promega,E2261 Notch1 luciferase reporter,Addgene,41726 Mouse recombinant Wnt-3a,R&D,1324-WNP Human recombinant

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pRL-CMV Vector

pRL-CMV Vector from Promega

Description

Specifications/Features

Promega

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Reviews

pRL-CMV Vector from Promega

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