pRL-CMV Vector from Promega

Product Specs
ItempRL-CMV Vector
CompanyPromega
Price $77.00Supplier Page View Company Product Page
Catalog NumberE2261
HostMammalian
Vector NamepRL-CMV
ResistanceAmpR
ReporterRenilla luciferase
Quantity20microg
PromoterCMV Enhancer and Immediate early promoter
Molecular Weight~66kDa
Selectable MarkerAmpicillin

Specifications/Features

Vector Size:
4079 bp

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(41) A suboptimal OCT4-SOX2 binding site facilitates the naïve-state specific function of a Klf4 enhancerPLOS ONESeptember 30, 2024Jack B. Waite, RuthMabel Boytz, Alexis R. Traeger, Torrey M. Lind, Koya Lumbao-Conradson, Sharon E. Torigoeluciferase reporter constructs (500 ng) and the CMV-Renilla luciferase reporter plasmid (10 ng; Promega E2261). Cells were harvested 21–24 hours post-transfection and assayed using the Dual Luciferase Reporter

(42) Non-canonical Wnt signaling triggered by WNT2B drives adrenal aldosterone productionbioRxivAugust 31, 2024Kleiton S. Borges, Donald W. Little, Taciani de Almeida de Almeida Magalhães, Claudio Ribeiro, Typhanie Dumontet, Chris Lapensee, Kaitlin J. Basham, Aishwarya Seth, Svetlana Azova, Nick A. Guagliardo, et al.luciferase reporter (SRF-RE) and the renilla luciferase thymidine kinase (pRL-TK) reporter (Promega, E2261) were co-transfected into HEK-293 (8×10^3 cells/well) cells cultured in a 96-well plate for 24h and

(43) The genomic basis of childhood T-lineage acute lymphoblastic leukaemiaNatureAugust 29, 2024Petri Pölönen, Danika Di Giacomo, Anna Eames Seffernick, Abdelrahman Elsayed, Shunsuke Kimura, Francesca Benini, Lindsey E. Montefiori, Brent L. Wood, Jason Xu, Changya Chen, et al.RBPJ/CSL luciferase reporter construct (pGa981-6)40 and Renilla luciferase expressing vector (pRL-CMV) (#E2261, Promega). Luciferase assays were performed using Dual Luciferase Assay System (#E1910, Promega) 24

(44) Identification of an alternative ligand‐binding pocket in peroxisome proliferator‐activated receptor gamma and its correlated selective agonist for promoting beige adipocyte differentiationMedComm (2020)July 10, 2024Qiang Tian, Miaohua Wang, Xueting Wang, Zhenli Lei, Owais Ahmad, Dianhua Chen, Wei Zheng, Pingping Shen, Nanfei Yang(S0033S, Beyotime), and Griess reagent (S0024, Beyotime), were purchased. PPRE‐luc (E4121) and pRL (E2261) plasmids were purchased from Promega. The cDNA sequence of mouse PPARγ was cloned into the pLenti6/v5

(45) Obesity disrupts the pituitary-hepatic UPR communication leading to NAFLD progressionCell MetabolismJuly 2, 2024Qingwen Qian, Mark Li, Zeyuan Zhang, Shannon W. Davis, Kamal Rahmouni, Andrew W. Norris, Huojun Cao, Wen-Xing Ding, Gökhan S. Hotamisligil, Ling YangATF6LD-Cluc-Gluc35 or UPRE-luciferase reporter36 with 0.15 μg/well Renilla luciferase vector (Promega, Cat No. E2261) using polyethylenimine (PEI, Polysciences INC, Cat No. 23966). At 48 hrs post-transfection, the activities

(46) Tripartite motif 8 promotes the progression of hepatocellular carcinoma via mediating ubiquitination of HNF1αCell Death & DiseaseJune 15, 2024Yu Peng, Hui Qian, Wen-Ping Xu, Meng-Chao Xiao, Chen-Hong Ding, Fang Liu, Huan-Yu Hong, Shu-Qing Liu, Xin Zhang, Wei-Fen Xieassay Cells transfected with HNF1α reporter plasmid pGL3-HNF4a-P2 and the control pRL-SV40 vector (E2261, Promega, USA) together with the plasmid overexpressing TRIM8 or the control plasmid were plated in

(47) CRISPR-based editing strategies to rectify EYA1 complex genomic rearrangement linked to haploinsufficiencyMolecular Therapy - Nucleic AcidsJune 11, 2024Hwalin Yi, Yejin Yun, Won Hoon Choi, Hye-Yeon Hwang, Ju Hyuen Cha, Heeyoung Seok, Jae-Jin Song, Jun Ho Lee, Sang-Yeon Lee, Daesik Kimtransfected initially with three distinct plasmids: pGL4.12[luc2CP]-MYOG-6xMEF3, pRK5-SIX1, and pRL/CMV (E2261, Promega, Madison, WI, USA). After transfection, the cells were harvested for a luciferase assay using

(48) Tripartite Motif-Containing Protein 65 (TRIM65) Inhibits Hepatitis B Virus TranscriptionVirusesMay 31, 2024Sheng Shen, Ran Yan, Zhanglian Xie, Xiaoyang Yu, Hongyan Liang, Qiuhong You, Hu Zhang, Jinlin Hou, Xiaoyong Zhang, Yuanjie Liu, et al.previously [45]. The CMV promoter Renilla–luciferase reporter plasmid pRL-CMV was purchased from Promega (#E2261). The cells were transfected with the indicated plasmid(s) using Lipofectamine 3000 (Life Technologies)

(49) Whole-genome sequencing uncovers two loci for coronary artery calcification and identifies ARSE as a regulator of vascular calcificationNature Cardiovascular ResearchMay 31, 2024Paul S. de Vries, Matthew P. Conomos, Kuldeep Singh, Christopher J. Nicholson, Deepti Jain, Natalie R. Hasbani, Wanlin Jiang, Sujin Lee, Christian L. Lino Cardenas, Sharon M. Lutz, et al.rs5982944-G allele constructs using lipofectamine 3000 (#L3000015, Invitrogen). Renilla plasmid (pRL-CMV, #E2261, Promega) was co-transfected with the luciferase constructs (1:100). Cells were harvested 72 hr post-transfection

(50) SRY-Box transcription factor 9 triggers YAP nuclear entry via direct interaction in tumorsSignal Transduction and Targeted TherapyApril 24, 2024Hui Qian, Chen-Hong Ding, Fang Liu, Shi-Jie Chen, Chen-Kai Huang, Meng-Chao Xiao, Xia-Lu Hong, Ming-Chen Wang, Fang-Zhi Yan, Kai Ding, et al.SOX9 together with a TEAD4-related reporter plasmid, 8×GTIIC (RIKEN, Japan). The pRL-SV40 (Promega, E2261) vector was transfected into all the treated cells and was used to normalize the data. Luciferase reporter

(51) CircPHGDH downregulation decreases papillary thyroid cancer progression through miR-122-5p/PKM2 axisBMC CancerApril 23, 2024Jiying Shen, Zhirong Ma, Jin Yang, Tianzhen Qu, Yu Xia, Yingjie Xu, Ming Zhou, Weiwei Liudatabase. Fragment sequences containing potential binding sites were inserted into pmirGLO vector (#E2261, Promega, Madison, WI, USA). Then, miR-122-5p mimics/negative control (NC) and pmirGLO vector were transfected

(52) Identification of constrained sequence elements across 239 primate genomesNatureJanuary 25, 2024Lukas F. K. Kuderna, Jacob C. Ulirsch, Sabrina Rashid, Mohamed Ameen, Laksshman Sundaram, Glenn Hickey, Anthony J. Cox, Hong Gao, Arvind Kumar, Francois Aguet, et al.of pGL3-enhancer, pGL3-control, or pGL3-promoter was co-transfected with 10 ng of pRL-CMV (Promega, E2261) as an internal control for the normalization of luciferase activity. Cells were incubated with DNA–lipid

(53) Surprising Features of Nuclear Receptor Interaction Networks Revealed by Live Cell Single Molecule ImagingbioRxivOctober 10, 2023Liza Dahal, Thomas GW Graham, Gina M Dailey, Alec Heckert, Robert Tjian, Xavier DarzacqRRID:Addgene_13458; Hoffman et al., 2006 was used for luciferase assays. A pRL-SV40 vector (Promega #E2261) expressing Renilla luciferase with SV40 promoter was used as a control to normalize luciferase activity.

(54) Critical Assessment of Condensate Boundaries in Dual-Color Single Particle TrackingThe Journal of Physical Chemistry BSeptember 14, 2023Guoming Gao, Nils G. Walterprotocol34. Briefly, the FL encoding sequence is amplified by PCR from a pRL-CMV plasmid (Promega, Cat# E2261) while adding a T7 promoter sequence (5’-TAATACGACTCACTATAGGG-3’) in the upstream primer. The Kozak

(55) Low level of ARID1A contributes to adaptive immune resistance and sensitizes triple-negative breast cancer to immune checkpoint inhibitorsCancer communications (London, England)September 1, 2023Chen XY, Li B, Wang Y, Jin J, Yang Y, Huang LH, Yang MD, Zhang J, Wang BY, Shao ZM, et al.reporter plasmids (Genechem). The cells were cotransfected with 10 ng of pRL‐CMV (Renilla luciferase, E2261, Promega, Madison, WI, USA) for normalization. At 48 h post transfection, the luciferase activity was

(56) EMT activates exocytotic Rabs to coordinate invasion and immunosuppression in lung cancerProceedings of the National Academy of Sciences of the United States of AmericaJuly 11, 2023Xiao GY, Tan X, Rodriguez BL, Gibbons DL, Wang S, Wu C, Liu X, Yu J, Vasquez ME, Tran HT, et al.Rabin8 (#3834008) from Applied Biological Materials; pRL Renilla-CMV Luciferase Control Reporter (#E2261) and pGL3 Firefly Luciferase Reporter (Basic) (#E1751) from Promega. Cell Culture. Murine and human

(57) Severe allergic dysregulation due to a gain of function mutation in the transcription factor STAT6Journal of Allergy and Clinical ImmunologyJuly 1, 2023Safa Baris, Mehdi Benamar, Qian Chen, Mehmet Cihangir Catak, Mónica Martínez-Blanco, Muyun Wang, Jason Fong, Michel J. Massaad, Asena Pinar Sefer, Altan Kara, et al.activity. The pRL-SV40 Renilla luciferase control reporter vector was obtained from Promega (catalogue no. E2261) and was used in co-transfection studies with p4xSTAT6-Luc2P for internal normalization. HEK293 cells

(58) Identification and functional validation of an enhancer variant in the 9p21.3 locus associated with glaucoma risk and elevated expression of p16 INK4abioRxivMay 25, 2023Yizhou Zhu, Cagdas Tazearslan, Michael G. Rosenfeld, Andras Fiser, Yousin Suh(ThermoFisher). Five hundred nanograms of vector were co-transfected with 25 ng CMV-Renilla (Promega E2261) into subject cell lines in 24-well plates at 70% confluence using Lipofectamine 3000 reagent. After

(59) CRISPR-Cas9 screen reveals a role of purine synthesis for estrogen receptor α activity and tamoxifen resistance of breast cancer cellsScience AdvancesMay 12, 2023Dina Hany, Vasiliki Vafeiadou, Didier PicardCRE-Luc (Stratagene), and the Renilla luciferase transfection control reporter pRL-CMV (Promega, no. E2261). To visualize PKA activity, plasmid pcDNA3-PKA-SPARK was used. It was a gift from X. Shu (Addgene,

(60) Implication of the Transmembrane Domain in the Interleukin 10 Receptor Platform OligomerisationCellsMay 10, 2023Thomas Kuntzel, Caroline Spenlé, Lucas D. Pham-Van, Dafni Birmpili, Aurélien Riou, Aurore Loeuillet, Imane Charmarke-Askar, Dominique Bagnardcloned into plasmids, which encoded either the Renilla luciferase Rluc (as a donor, plasmid pRL-CMV E2261, Promega, Madison, WI, USA) or the enhanced yellow fluorescent protein eYFP (as an acceptor, plasmid

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pRL-CMV Vector

pRL-CMV Vector from Promega

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Specifications/Features

Promega

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pRL-CMV Vector from Promega

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