pRL-CMV Vector from Promega

Product Specs
ItempRL-CMV Vector
CompanyPromega
Price $77.00Supplier Page View Company Product Page
Catalog NumberE2261
HostMammalian
Vector NamepRL-CMV
ResistanceAmpR
ReporterRenilla luciferase
Quantity20microg
PromoterCMV Enhancer and Immediate early promoter
Molecular Weight~66kDa
Selectable MarkerAmpicillin

Specifications/Features

Vector Size:
4079 bp

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(61) Structural features of the Notch ankyrin domain-Deltex WWE2 domain heterodimer determined by NMR spectroscopy and functional implicationsStructureMay 1, 2023Andrea A. Carter, Kristen M. Ramsey, Christine L. Hatem, Kathryn P. Sherry, Ananya Majumdar, Doug Barrick(TP-1/pGa981-6),RIKEN BRC,"Minoguchi et al., Kurooka et al. 46,47" pRL-CMV Renilla luciferase vector,Promega,Cat #: E2261 Software and algorithms,Software and algorithms,Software and algorithms nmrPipe,NIST IBBR 48,https://www.ibbr.umd.edu/nmrpipe/ CARA,Swiss

(62) Protein kinase B (PKB/AKT) protects IDH-mutated glioma from ferroptosis via Nrf2Clin Cancer ResApril 3, 2023Yang Liu, Fu-Ju Chou, Fengchao Lang, Meili Zhang, Hua Song, Wei Zhang, Dionne L. Davis, Nicole J. Briceno, Yang Zhang, Patrick J. Cimino, et al.nanogram of plasmid pGL4.37[luc2P/ARE/Hygro] (Promega, E3641) was mixed with 5 ng pRL-CMV (Promega, E2261) and EGFP-Nrf2 plasmids (Addgene 21549). The plasmid mixtures were transfected into 104 cells using

(63) Protein Kinase B (PKB/AKT) Protects IDH-Mutated Glioma from Ferroptosis via Nrf2Clinical Cancer ResearchApril 3, 2023Yang Liu, Fu-Ju Chou, Fengchao Lang, Meili Zhang, Hua Song, Wei Zhang, Dionne L. Davis, Nicole J. Briceno, Yang Zhang, Patrick J. Cimino, et al.nanogram of plasmid pGL4.37[luc2P/ARE/Hygro] (Promega, E3641) was mixed with 5 ng pRL-CMV (Promega, E2261) and EGFP-Nrf2 plasmids (Addgene 21549). The plasmid mixtures were transfected into 104 cells using

(64) Network-informed discovery of multidrug combinations for ERα+/HER2-/PI3Kα-mutant breast cancerCellular and Molecular Life SciencesMarch 3, 2023Dina Hany, Marloes Zoetemelk, Kaushik Bhattacharya, Patrycja Nowak-Sliwinska, Didier Picardco-transfected with 50 ng of the Renilla luciferase transfection control plasmid pRL-CMV (Promega #E2261) and 1 μg of either the ERα reporter plasmid XETL (here referred to as ERE-Luc) [20] or the HIF1α reporter

(65) TGFβ-mediated MMP13 secretion drives myoepithelial cell dependent breast cancer progressionnpj Breast CancerMarch 2, 2023Shayin V. Gibson, Elena Tomas Bort, Lucía Rodríguez-Fernández, Michael D. Allen, Jennifer J. Gomm, Iain Goulding, Ulrich auf dem Keller, Andrea Agnoletto, Cathrin Brisken, Barrie Peck, et al.per well of a 24-well plate were co-transfected with 170 ng Renilla luciferase control plasmid (E2231/E2261, pRL, Promega) and 330 ng of reporter plasmid encoding SBE-Firefly luciferase with Lipofectamine LTX/Plus reagent

(66) Identification of long non-coding RNA and circular RNA associated networks in cellular stress responsesFrontiers in GeneticsFebruary 10, 2023Xiuzhi Li, Jingxin Li, Ge Shan, Xiaolin WangsiRNAs, 1 μg pGL3 Firefly luciferase plasmids and 100 ng pRL Renilla luciferase reporter vector (Promega, E2261). After transfection for 48 h, cells were lysed with passive lysis buffer on ice for 20 min and the

(67) Myelodysplastic Syndrome associated TET2 mutations affect NK cell function and genome methylationNature CommunicationsFebruary 3, 2023Maxime Boy, Valeria Bisio, Lin-Pierre Zhao, Fabien Guidez, Bérénice Schell, Emilie Lereclus, Guylaine Henry, Juliette Villemonteix, Fernando Rodrigues-Lima, Katia Gagne, et al.with the 2.7 µg TET2 plasmids were co-transfected in HEK293T cells with 0.06 µg of pRL-CMV Vector (#E2261 Promega) in presence of PEI solution. In parallel, Jurkat cells (ATCC) were transfected with 0.26 µg

(68) CEACAM1 is a direct SOX10 target and inhibits melanoma immune infiltration and stemnessiScienceDecember 22, 2022Abou-Hamad J, Hodgins JJ, de Souza CT, Garland B, Labrèche C, Marotel M, Gibson C, Delisle S, Pascoal J, Auer RC, et al.Ceacam1[-4135/-2785],This manuscript,N/A pGL3P Ceacam1[-5305/-4045],This manuscript,N/A pRL-CMV,Promega,Cat# E2261 pLenti Cas9-Blast,Dr. Daniel Schramek; University of Toronto,N/A pLKO H2B-mRFP-2A puro,Dr. Daniel

(69) Shear stress switches the association of endothelial enhancers from ETV/ETS to KLF transcription factor binding sitesScientific ReportsDecember 1, 2022Roman Tsaryk, Nora Yucel, Elvin V. Leonard, Noelia Diaz, Olga Bondareva, Maria Odenthal-Schnittler, Zoltan Arany, Juan M. Vaquerizas, Hans Schnittler, Arndt F. Siekmannand PLUS reagent (Invitrogen, # 11514015) with a firefly plasmid and the pRL-CMV plasmid (Promega, # E2261) containing the Renilla luciferase gene for normalization. HUVEC were exposed to shear stress (18 dyn/cm2

(70) Recovering mixtures of fast-diffusing states from short single-particle trajectorieseLifeSeptember 6, 2022Alec Heckert, Liza Dahal, Robert Tijan, Xavier DarzacqMichael Underhill (Hoffman et al., 2006). Renilla luciferase was expressed from pRL CMV Renilla (Promega E2261). Cell lines Human U2OS cells (female, 15 years old, osteosarcoma) obtained from the UC Berkeley Cell Culture

(71) Recovering mixtures of fast-diffusing states from short single-particle trajectoriesElifeSeptember 6, 2022Alec Heckert, Liza Dahal, Robert Tjian, Xavier DarzacqMichael Underhill (Hoffman et al., 2006). Renilla luciferase was expressed from pRL CMV Renilla (Promega E2261). Cell lines Human U2OS cells (female, 15 years old, osteosarcoma) obtained from the UC Berkeley Cell Culture

(72) Transcription factor Creb3l1 maintains proteostasis in neuroendocrine cellsMolecular MetabolismSeptember 1, 2022Mingkwan Greenwood, Benjamin T. Gillard, Rizwan Farrukh, Alex Paterson, Ferdinand Althammer, Valery Grinevich, David Murphy, Michael P. Greenwood(Creb3l1CA) or empty pcDNA3.1 (0.45 μg/well), and control renilla reporter pRL-CMV (1 ng/well, Promega, E2261). Samples were collected 48 h post transfection. 2.7 Virus production and validation Creb3l1 and non-targeting

(73) Genes with 5′ terminal oligopyrimidine tracts preferentially escape global suppression of translation by the SARS-CoV-2 Nsp1 proteinbioRxivJune 28, 2022Shilpa Rao, Ian Hoskins, Tori Tonn, P. Daniela Garcia, Hakan Ozadam, Elif Sarinay Cenik, Can CenikNM_001636) were custom synthesized (IDT) and cloned into pRL-CMV Renilla luciferase vector (Promega, E2261) using oligos in Supplementary file 1. Briefly, pRL-CMV was digested with NdeI and NheI followed by

(74) H2O2 down-regulates SIRT7''s protective role of endothelial premature dysfunction via microRNA-335-5pBioscience reportsMay 27, 2022Liu Y, Yang J, Yang X, Lai P, Mou Y, Deng J, Li X, Wang H, Liu X, Zhou L, et al.mixture of the miRNA mimic or the mimic control pYr-KL 3′-UTR, and Renilla luciferase vector pRL-CMV (E2261, Promega, WI, U.S.A.) using the Lipofectamine 2000 (11668030, Invitrogen, CA, U.S.A.) transfection system.

(75) Vinexin contributes to autophagic decline in brain ageing across speciesCell death and differentiationMay 1, 2022Park SJ, Frake RA, Karabiyik C, Son SM, Siddiqi FH, Bento CF, Sterk P, Vicinanza M, Pavel M, Rubinsztein DC.obtained from Addgene. The Renilla luciferase control reporter (pRL-CMV) was purchased from Promega (E2261). Pre-designed pLKO.1 shRNAs vectors were acquired from The RNAi Consortium (TRC) (empty vector control;

(76) ETV2 regulates PARP-1 binding protein to induce ER stress–mediated death in tuberin-deficient cellsLife Science AllianceMay 1, 2022Shikshya Shrestha et al.reagent (Sigma-Aldrich). Cells were transfected with 10 ng of pRL_CMV vector (internal control, Cat. no. E2261; Promega) and 140 ng of promoter constructs. Luciferase activity was tested 24 h after plasmid transfections

(77) Cooperative interaction between ERα and the EMT-inducer ZEB1 reprograms breast cancer cells for bone metastasisNature communicationsApril 19, 2022Mohammadi Ghahhari N, Sznurkowska MK, Hulo N, Bernasconi L, Aceto N, Picard D.Addgene #16495)102, and the renilla luciferase transfection control reporter pRL-CMV from Promega (E2261). For knockdowns of TFAP2C, FOXA1, MUC16, DIO2, ESR2, P2RX7, MMP10, SCG2, MUC2, DSCAM, CD151, ANXA2,

(78) ADAP1 promotes latent HIV-1 reactivation by selectively tuning KRAS-ERK-AP-1 T cell signaling-transcriptional axisNature communicationsMarch 1, 2022Ramirez NP, Lee J, Zheng Y, Li L, Dennis B, Chen D, Challa A, Planelles V, Westover KD, Alto NM, et al.pGL3-NF-κB-luciferase (Promega), pGL3-NFAT-luciferase (Addgene, 17870)) and 0.2 μg Renilla plasmid (pRL-CMV, Promega, E2261) at room temperature. Triplicate reactions were set up for each reporter plasmid. Cells were transferred

(79) Rapid Assessment of Insect Steroid Hormone Entry Into Cultured CellsFrontiers in PhysiologyJanuary 26, 2022Mitchell Masterson, Riyan Bittar, Hannah Chu, Naoki Yamanaka, Sachiko Haga-Yamanaka(Agilent Technologies, 217468), and 250 ng/well of pRL-CMV Renilla luciferase reporter plasmid (Promega, E2261) as a reference. After 2 days of incubation in the conditions described above, the cells were washed

(80) EGFR Inhibition Enhances the Cellular Uptake and Antitumor-Activity of the HER3 Antibody–Drug Conjugate HER3–DXdCancer ResearchJanuary 1, 2022Heidi M. Haikala et al.Addgene #60899) and 100ng of the Renilla luciferase-expressing pRL-CMV control plasmid (Promega, Cat# E2261) for 24hrs using Fugene HD transfection reagent (Promega Cat#E2311). Cells were then drug treated and

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pRL-CMV Vector

pRL-CMV Vector from Promega

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Promega

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pRL-CMV Vector from Promega

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