pRL-CMV Vector from Promega

Product Specs
ItempRL-CMV Vector
CompanyPromega
Price $77.00Supplier Page View Company Product Page
Catalog NumberE2261
HostMammalian
Vector NamepRL-CMV
ResistanceAmpR
ReporterRenilla luciferase
Quantity20microg
PromoterCMV Enhancer and Immediate early promoter
Molecular Weight~66kDa
Selectable MarkerAmpicillin

Specifications/Features

Vector Size:
4079 bp

Add to Compare List

Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(21) Hydrogel-embedded precision-cut lung slices support ex vivo culture of in vivo-induced premalignant lung lesionsPhysiol RepJuly 12, 2025Caroline Hauer, Rachel Blomberg, Kayla Sompel, Chelsea M. Magin, Meredith A. Tennisluciferase (Addgene plasmid #1015; gift from Bruce Speigelman) and 5 ng renilla control reporter vector (#E2261, Promega), mock luciferase, and empty vector. 24 h after transfection, 15 μL of media was collected

(22) CLU alleviates Alzheimer’s disease-relevant processes by modulating astrocyte reactivity and microglia-dependent synaptic densityNeuronJune 18, 2025Alexandra M. Lish, Elyssa F.L. Grogan, Courtney R. Benoit, Richard V. Pearse, Sarah E. Heuer, Tain Luquez, Gwendolyn A. Orme, Paige C. Galle, Giedre Milinkeviciute, Kim N. Green, et al.pGL4.32[luc2P/NF-κB-RE/Hygro], encoding Firefly luciferase under a minimal promoter with an NfκB response element (Promega, #E2261 and #E849A, respectively). Day 18 iAs were transfected with the two reporter plasmids using the Amaxa

(23) TFAP2C Drives Cisplatin Resistance in Bladder Cancer by Upregulating YAP and Activating β-Catenin SignalingJournal of Biological ChemistryJune 18, 2025Qiufeng Pan, Changmin Zou, Zhigen Lin, Hao Tang, Zepu Long, Longwang Wangeither an empty vector or a TFAP2C expression plasmid, along with a Renilla luciferase control plasmid (#E2261, Promega) for normalization. After 48 h, cells were harvested and lysed, and luciferase activity was

(24) A niche driven mechanism determines response and a mutation-independent therapeutic approach for myeloid malignanciesCancer cellJune 9, 2025Mosialou I, Ali AM, Labella R, Bisikirska B, Cuesta-Dominguez A, Vgenopoulou P, Reyes I, Rao SM, Wang A, Luo N, et al.serum,Jackson ImmunoResearch,017-000-121 Lipofectamine 3000,Invitrogen,L3000015 pRL-CMV Renilla,Promega,E2261 Notch1 luciferase reporter,Addgene,41726 Mouse recombinant Wnt-3a,R&D,1324-WNP Human recombinant

(25) Auricular malformations are driven by copy number variations in a hierarchical enhancer cluster and a dominant enhancer recapitulates human pathogenesisNat CommunMay 17, 2025Xiaopeng Xu, Qi Chen, Qingpei Huang, Timothy C. Cox, Hao Zhu, Jintian Hu, Xi Han, Ziqiu Meng, Bingqing Wang, Zhiying Liao, et al.promoter (Promega, catalog no. E1761), 0.02 μg of pRL-CMV Renilla luciferase (Promega, catalog no. E2261) as an internal control, and 1.5 μL of FuGENE HD (Promega, catalog no. E2691), all diluted in Opti-MEM I Reduced-Serum Medium

(26) Promoter strength and position govern promoter competitionbioRxivMay 10, 2025Mervenaz Koska, Tomek Swigut, Alistair Nicol Boettiger, Joanna Wysockawith each well receiving 10ng of pGL3 plasmid, 0.5ng of control pRL firefly renilla plasmid (Promega E2261), 89.5 mL carrier DNA (circularized pGEMT plasmid) and 0.25ul of Lipofectamine in 50ul Optimem (Gibco

(27) Discovery of a novel binding pocket in PPARγ for partial agonists: structure-based virtual screening identifies ginsenoside Rg5 as a partial agonist promoting beige adipogenesisFront ChemMay 8, 2025Zhen Wang, Kexin Shui, Zehui Zhang, Yihan Chen, Nanfei Yang, Shiliang Ji, Pingping Shen, Qiang TianqPCR SYBR Green Master Mix kit (Q111-02, Vazyme, China), were purchased. PPRE-luc (E4121) and pRL (E2261) plasmids were purchased from Promega. The cDNA sequence of PPARγ was cloned into the pLenti6/v5 lentiviral

(28) Amplified dosage of the NKX2-1 lineage transcription factor controls its oncogenic role in lung adenocarcinomaMolecular CellApril 3, 2025John L. Pulice, Matthew MeyersonLab,RRID:Addgene_12260 pCMV-VSV-G,"Stewart et al., (2003)111",RRID:Addgene_8454 pGL4.23,Promega,CAT#E8411 pRL-CMV,Promega,CAT#E2261 pLenti-dCas9-KRAB-MeCP2-Blast,"Liu et al., 202199",RRID:Addgene_221501 lentiGuide-Puro,"Sanjana et

(29) Phosphorylation of eIF2α suppresses the impairment of GSH/NADPH homeostasis and mitigates the activation of cell death pathways, including ferroptosis, during ER stressMolecules and CellsMarch 13, 2025Hien Thi Thi Le, Yong Hwan Kim, Mi-Jeong Kim, Seung Hwa Hyun, Hyeeun Kim, Su Wol Chung, Yeonsoo Joe, Hun Taeg Chung, Dong-Myung Shin, Sung Hoon Backpyralis). The pRL-CMV plasmid expressing CMV promoter-driven Renilla luciferase was purchased from Promega (E2261). To generate a plasmid expressing both hemagglutinin (HA)-tagged ATF4 and the nuclear-localized near-infrared

(30) The Adhesion GPCR ADGRL2 engages Gα13 to Enable Epidermal DifferentiationbioRxivMarch 1, 2025Xue Yang, Feng He, Douglas F. Porter, Krassimira Garbett, Robin M. Meyers, David L. Reynolds, Duy Lan Huong Huong Bui, Audrey Hong, Luca Ducoli, Zurab Siprashvili, et al.Biotechnol." TRUPATH kit,Addgene,1000000163 SRF-RE reporter plasmid,Promega,E1350 pRL-Renilla plasmid,Promega,E2261 pLEX CMV Cas9 blast,This study,N/A F+E 10x cs1 sgRNA mCherry,This study,N/A Plex Flag-Keratin14,This

(31) Evolutionary conserved regulation of TFEB stability by the E3 ubiquitin ligase WWP2 modulates response to stress in vivoiScienceFebruary 21, 2025Juan A. Garcia-Sanchez, Estelle Bonnet, Céline Loubatier, Anne Doye, Guillaume Paillier, Fabien Segui, Frédéric Larbret, Paul Chaintreuil, Ludovic Batistic, Cédric Torre, et al.4XCLEAR-luciferase reporter,Cortes et al.72,RRID: Addgene_66800 Plasmid: pRL Renilla Luciferase,Promega,Cat #E2261 ,, Software and algorithms,Software and algorithms,Software and algorithms ,, GeneSys V1.5.4.0,Syngene,RRID:

(32) A distal convoluted tubule-specific isoform of murine SLC41A3 extrudes magnesiumActa Physiol (Oxf)February 11, 2025Gijs A. C. Franken, Willem Bosman, Hyun Jun Jung, Caro Bos, Femke Latta, Mark Knepper, Joost G. J. Hoenderop, Jeroen H. F. de BaaijCommercially available plasmids encoding Firefly luciferase (Promega, E1751) and Renilla luciferase (Promega, E2261) were used for the luciferase assay. The 477 bp Slc41a3‐Iso 2 cis‐regulatory element (CRE), the 1000

(33) BANCR-Containing Extracellular Vesicles Enhance Breast Cancer ResistanceJournal of Biological ChemistryFebruary 11, 2025Xinming Song, Shen Liu, Ying Zeng, Yilin Cai, Haiqing Luocotransfected with the 300 ng constructed pMIR-REPORT plasmid, 10 ng pRL-CMV (renilla luciferase gene vector, E2261, Promega), and 50 nM mimic-NC and miR-34a-5p mimic using Lipofectamine 3000 reagent (L3000001, Thermo Fisher Scientific

(34) Surprising Features of Nuclear Receptor Interaction Networks Revealed by Live Cell Single Molecule ImagingElifeJanuary 10, 2025Liza Dahal, Thomas G W Graham, Gina M Dailey, Alec Heckert, Robert Tjian, Xavier DarzacqRRID:Addgene_13458; Hoffman et al., 2006) was used for luciferase assays. A pRL-SV40 vector (Promega #E2261) expressing Renilla luciferase with SV40 promoter was used as a control to normalize luciferase activity.

(35) Surprising features of nuclear receptor interaction networks revealed by live-cell single-molecule imagingElifeJanuary 10, 2025Liza Dahal, Thomas GW Graham, Gina M Dailey, Alec Heckert, Robert Tjian, Xavier DarzacqRRID:Addgene_13458; Hoffman et al., 2006) was used for luciferase assays. A pRL-SV40 vector (Promega #E2261) expressing Renilla luciferase with SV40 promoter was used as a control to normalize luciferase activity.

(36) Cardiac fibroblast BAG3 regulates TGFBR2 signaling and fibrosis in dilated cardiomyopathyThe Journal of clinical investigationJanuary 2, 2025Wang BZ, Morsink MA, Kim SW, Luo LJ, Zhang X, Soni RK, Lock RI, Rao J, Kim Y, Zhang A, et al.described. Twenty-four hours later, cells were transfected with SBE4-Luc (Addgene 16495) and pRL (Promega E2261) at a 1:1 ratio using Viafect as described. Twenty-four hours after transfection, medium was refreshed

(37) BMP-9 mediates fibroproliferation in fibrodysplasia ossificans progressiva through TGF-β signalingEMBO molecular medicineJanuary 1, 2025Zhao C, Inada Y, Motoike S, Kamiya D, Hino K, Ikeya M.B,Toyobo,NKB-601 Tween-20,Sigma-Aldrich,P9416 Cardiotoxin,Latoxan,L8102 GDF2/BMP-9 ELISA Kit,LifeSpan BioSciences,LS-F3779 pRL-CMV-Renilla luciferase reporter,Promega Corporation,E2261 Dual-luciferase reporter assay system,Promega,E1910 Silencer® Select Pre-designed siRNA,Thermo Fisher Scientific

(38) Cell-permeated peptide P-T3H2 inhibits malignancy on hepatocellular carcinoma through stabilizing HNF4α proteinDiscover OncologyDecember 5, 2024Si-Han Wu, Meng-Chao Xiao, Fang Liu, Huan-Yu Hong, Chen-Hong Ding, Xin Zhang, Wei-Fen XieμM of P-T3H2-2A or P-T3H2 for 48 h in 96-well plates. To normalize the data, the pRL-SV40 (Promega, E2261) vector was transfected into all the treated cells. Luciferase reporter gene assays were performed using

(39) Identification of CRTH2 as a New PPARγ-Target Gene in T Cells Suggested CRTH2 Dependent Conversion of Th2 Cells as Therapeutic Concept in COVID-19 InfectionImmunotargets TherNovember 2, 2024Antonia Becker, Karoline Röhrich, Amanda Leske, Ulrike Heinicke, Tilo Knape, Aimo Kannt, Verena Trümper, Kai Sohn, Annett Wilken-Schmitz, Holger Neb, et al.transfection efficiency a vector encoding renilla luciferase (RL) driven by a CMV promoter (pRL-CMV, #E2261, Promega, Walldorf, Germany) was also transfected. Cells were treated as indicated. After harvesting

(40) Identification of CRTH2 as a New PPARγ-Target Gene in T Cells Suggested CRTH2 Dependent Conversion of Th2 Cells as Therapeutic Concept in COVID-19 InfectionImmunoTargets and TherapyNovember 2, 2024Antonia Becker, Karoline Röhrich, Amanda Leske, Ulrike Heinicke, Tilo Knape, Aimo Kannt, Verena Trümper, Kai Sohn, Annett Wilken-Schmitz, Holger Neb, et al.transfection efficiency a vector encoding renilla luciferase (RL) driven by a CMV promoter (pRL-CMV, #E2261, Promega, Walldorf, Germany) was also transfected. Cells were treated as indicated. After harvesting

Be the First to Write a Review!

pRL-CMV Vector

pRL-CMV Vector from Promega

Description

Specifications/Features

Promega

Citations (189)

Reviews

pRL-CMV Vector from Promega

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map