pRL-CMV Vector from Promega

Product Specs
ItempRL-CMV Vector
CompanyPromega
Price $77.00Supplier Page View Company Product Page
Catalog NumberE2261
HostMammalian
Vector NamepRL-CMV
ResistanceAmpR
ReporterRenilla luciferase
Quantity20microg
PromoterCMV Enhancer and Immediate early promoter
Molecular Weight~66kDa
Selectable MarkerAmpicillin

Specifications/Features

Vector Size:
4079 bp

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(101) R269C variant of ESR1: high prevalence and differential function in a subset of pancreatic cancersBMC CancerDecember 1, 2020Tomer Boldes, Keren Merenbakh-Lamin, Shani Journo, Eliya Shachar, Doron Lipson, Adva Yeheskel, Metsada Pasmanik-Chor, Tami Rubinek, Ido Wolfof the vitellogenin ERE promoter. pRL Renilla luciferase control was purchased from Promega (Cat no E2261, Promega, Madison, WI). The generation of WT-ER construct (in pcDNA3) was described elsewhere [39].

(102) MondoA regulates gene expression in cholesterol biosynthesis-associated pathways required for zebrafish epibolyElifeSeptember 24, 2020Meltem Weger, Benjamin D Weger, Andrea Schink, Masanari Takamiya, Johannes Stegmaier, Cédric Gobet, Alice Parisi, Andrei Yu Kobitski, Jonas Mertes, Nils Krone, et al.600 ng 2xChoRE reporter DNA, 200 ng pRL-CMV vector (to control for transfection efficiency; Promega #E2261), and a total of 200 ng of transcription factor and carrier DNA (pBluescript SK). For the overexpression

(103) MondoA regulates gene expression in cholesterol biosynthesis-associated pathways required for zebrafish epibolyeLifeSeptember 24, 2020Meltem Weger et al.600 ng 2xChoRE reporter DNA, 200 ng pRL-CMV vector (to control for transfection efficiency; Promega #E2261), and a total of 200 ng of transcription factor and carrier DNA (pBluescript SK). For the overexpression

(104) Evolution of Human Brain Size-Associated NOTCH2NL Genes Proceeds toward Reduced Protein LevelsMolecular Biology and EvolutionSeptember 1, 2020Gerrald A Lodewijk, Diana P Fernandes, Iraklis Vretzakis, Jeanne E Savage, Frank M J JacobsAddgene 51142) were cotransfected to measure effects of NOTCH2NL on reporter activity. pRL-CMV (Promega E2261) was used for normalization. For transfection, U2OS cells were seeded in six-well plates at a density

(105) Activating transcription factor 3 inhibits endometrial carcinoma aggressiveness via JunB suppressionInternational journal of oncologySeptember 1, 2020Wang F, Li J, Wang H, Zhang F, Gao J.5'untranslated region (UTR) of JunB was cloned into the pRL-CMV luciferase reporter plasmid (50 ng; cat. no. E2261; Promega Corporation) and the resultant vectors were designated as 'pRL-CMV-JunB-5'UTR-WT' and 'pRL-CMV-JunB-5'UTR-MT',

(106) FK506 induces lung lymphatic endothelial cell senescence and downregulates LYVE-1 expression, with associated decreased hyaluronan uptakeMolecular medicine (Cambridge, Mass.)July 31, 2020Shrestha S, Cho W, Stump B, Imani J, Lamattina AM, Louis PH, Pazzanese J, Rosas IO, Visner G, Perrella MA, et al.Reagent (Sigma). Cells were transfected with 5 ng of pRL_CMV vector (internal control, Promega, Cat# E2261) and 95 ng of P-124/+ 214 Shrestha et al. Molecular Medicine (2020) 26:75 (wildtype or mutant)

(107) 5' UTR variants in the quantitative trait gene Hnrnph1 support reduced 5' UTR usage and hnRNP H protein as a molecular mechanism underlying reduced methamphetamine sensitivityFASEB journal : official publication of the Federation of American Societies for Experimental BiologyJuly 1, 2020Ruan QT, Yazdani N, Reed ER, Beierle JA, Peterson LP, Luttik KP, Szumlinski KK, Johnson WE, Ash PEA, Wolozin B, et al.control plasmid expressing Renilla luciferase driven by the CMV promoter (Promega, Madison, WI, USA; Cat# E2261). 24 hours after transfection, HEK293T cells were detached with 0.25% of Trypsin-EDTA (ThermoFisher Scientific

(108) miR-484 is associated with disease recurrence and promotes migration in prostate cancerBioscience reportsMay 29, 2020Lee D, Tang W, Dorsey TH, Ambs S.pLenti-UTR-Luc PSMG1 WT or pLenti-UTR-Luc PSMG1 MUT, and 4 ng of pRL-CMV Renilla luciferase reporter (Cat# E2261) from Promega (Madison, WI), using TransIT-X2 (Cat# 6004) from Mirus (Madison, WI). Cells were cultured

(109) Myristoylation of LMCD1 Leads to Its Species-Specific Derepression of E2F1 and NFATc1 in the Modulation of CDC6 and IL-33 Expression During Development of Vascular LesionsArteriosclerosis, thrombosis, and vascular biologyMay 1, 2020Govatati S, Pichavaram P, Janjanam J, Guo L, Virmani R, Rao GN.No. 1345-25) was obtained from Click Chemistry Tools (Scottsdale, AZ). pRL-CMV vector (Catalog No. E2261) was bought from Promega (Madison, WI). Mouse LMCD1 (NM_144799) expression plasmid (Catalog No. MR205613)

(110) Allosteric Activators of Protein Phosphatase 2A Display Broad Antitumor Activity Mediated by Dephosphorylation of MYBL2CellApril 30, 2020Morita K, He S, Nowak RP, Wang J, Zimmerman MW, Fu C, Durbin AD, Martel MW, Prutsch N, Gray NS, et al.pGL4.20,Promega,Cat#E6751 Plasmid: pCDNA3 WT B-Myb,"Johnson et al., 1999",Addgene Plasmid #25965 Plasmid: pRL-CMV,Promega,Cat#E2261 Software and Algorithms,Software and Algorithms,Software and Algorithms GraphPad Prism (version 7),GraphPad,RRID:

(111) ASPP1 deficiency promotes epithelial-mesenchymal transition, invasion and metastasis in colorectal cancerCell death & diseaseApril 8, 2020Liu D, Ertay A, Hill C, Zhou Y, Li J, Zou Y, Qiu H, Yuan X, Ewing RM, Lu X, et al.plates, followed by 24 h transfection with 250 ng of NF-κB reporter and 10 ng of phRL-CMV (Promega, E2261), which constitutively expresses the Renilla luciferase reporter. One day before the measurement of

(112) RhoBTB Proteins Regulate the Hippo Pathway by Antagonizing Ubiquitination of LKB1G3 Genes|Genomes|GeneticsApril 1, 2020Thanh Hung Nguyen, Adela Ralbovska, Jan-Michael KuglershRNAs 8xGTIIC-luciferase was a gift from Stefano Piccolo (Addgene plasmid #34615). The pRL-CMV (Renilla, #E2261) was purchased from Promega (Madison, WI, USA). Smart pool siRNAs against RhoBTB2 were obtained from

(113) Mutant ACVR1 Arrests Glial Cell Differentiation to Drive Tumorigenesis in Pediatric GliomasCancer cellMarch 16, 2020Fortin J, Tian R, Zarrabi I, Hill G, Williams E, Sanchez-Duffhues G, Thorikay M, Ramachandran P, Siddaway R, Wong JF, et al.paper,N/A NanoLuc Protein Fusion MCS Vector,Promega,N1361 Renilla Luciferase Control Reporter Vector,Promega,E2261 6XE2F-Luciferase,Laboratory of Dr. Kristian Helin,N/A BRE-Luciferase,Laboratory of Dr. Peter ten Dijke,N/A CAGA-Luciferase,Laboratory

(114) Down-regulation of SLC35C1 induces colon cancer through over-activating Wnt pathwayJournal of cellular and molecular medicineMarch 1, 2020Deng M, Chen Z, Tan J, Liu H.plasmid was from EMD Millipore (#17‐285). The Renilla Luciferase control plasmid pRL was from Promega (#E2261). The integrity of all constructs was confirmed by gene sequencing. The shRNA‐SLC35C1 plasmids and control

(115) DIX Domain Polymerization Drives Assembly of Plant Cell Polarity ComplexesCellFebruary 6, 2020Maritza van Dop, Marc Fiedler, Sumanth Mutte, Jeroen de Keijzer, Lisa Olijslager, Catherine Albrecht, Che-Yang Liao, Marcel E. Janson, Mariann Bienz, Dolf Weijers8x TopFLASH,"Veeman et al., 2003",Addgene Cat#12456 Plasmid: pRL-CMV Renilla luciferase,Promega,Cat#E2261 Plasmid: pCTRN-nptII,"Hiwatashi et al., 2008",GenBank AB697058 Plasmid: pCTRN-nptII-PpSOK1-RF+LF,This

(116) Pathogenic FAM83G palmoplantar keratoderma mutations inhibit the PAWS1:CK1α association and attenuate Wnt signallingWellcome Open ResearchSeptember 9, 2019Kevin Z.L. Wu, Rebecca A. Jones, Theresa Tachie-Menson, Thomas J. Macartney, Nicola T. Wood, Joby Varghese, Robert Gourlay, Renata F. Soares, James C. Smith, Gopal P. Sapkotaseeded in 12-well plates. 24 h later, 200 ng of SuperTOPFlash, 20 ng of Renilla luciferase (Promega, E2261), and 100 ng of GFP or PAWS1-GFP plasmids were co-transfected as described above. After 24 h, cells

(117) Estrogen therapy induces an unfolded protein response to drive cell death in ER+ breast cancerMolecular oncologyAugust 1, 2019Hosford SR, Shee K, Wells JD, Traphagen NA, Fields JL, Hampsch RA, Kettenbach AN, Demidenko E, Miller TW.into cells along with a plasmid encoding CMV‐Renilla luciferase (Promega, Madison, WI, USA, catalog #E2261) as a transfection control. The next day, cells were treated as indicated, and luciferase activities

(118) Robust Normalization of Luciferase Reporter DataMethods and ProtocolsJuly 25, 2019Andrea Repele, Manucells were transfected with Cebpa promoter reporter vector and Renilla control vector pRL-CMV (Promega, E2261) in a 1:200 ratio using a 4D-Nucleofector (Lonza). Cells were transfected with 2.26 μg total plasmid

(119) Mutant CEBPA directly drives the expression of the targetable tumor-promoting factor CD73 in AMLScience AdvancesJuly 10, 2019Janus S. Jakobsen et al.white-bottom 96-well tissue culture plate. The following day, 0.2 ng of Renilla control vector (pRL-CMV, cat# E2261, Promega), 20 ng of empty or enhancer-containing promotor-pGL4 (pGL4:23, cat# E841A, Promega), and 20

(120) Common Regulatory Pathways Mediate Activity of MicroRNAs Inducing Cardiomyocyte ProliferationCell reportsMay 28, 2019Torrini C, Cubero RJ, Dirkx E, Braga L, Ali H, Prosdocimo G, Gutierrez MI, Collesi C, Licastro D, Zentilin L, et al.Piccolo, University of Padova","Addgene, Cat#34615" pRL Renilla Luciferase control plasmid,Promega,cat# E2261 5SA-YAP1 in pCSII-EF-MCS plasmid,"Gift from Stefano Piccolo, University of Padova",N/A psiCheck2.1

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pRL-CMV Vector

pRL-CMV Vector from Promega

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Specifications/Features

Promega

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pRL-CMV Vector from Promega

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