psiCHECK-2 Vector from Promega

Product Specs
ItempsiCHECK-2 Vector
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberC8021
Hostmammalian cell line of choice
PromoterSV40 early enhancer/promoter
ResistanceAmp
Quantity20microg
Molecular Weight~78kDa
Nucleic Acid TypeExpression Vectors
Expression System (standardized)Mammalian
Selectable Marker (standardized)Ampicillin
Vector NamepsiCHECK-2

Specifications/Features

Vector Size:
~6.3 kb

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(261) Passenger strand of miR-145-3p acts as a tumor-suppressor by targeting MYO1B in head and neck squamous cell carcinomaInternational Journal of OncologyNovember 6, 2017Yasutaka Yamada et al.inserted between the XhoI-PmeI restriction sites in the 3′-UTR of the hRluc gene in the psiCHECK-2 vector (C8021; Promega, Madison, WI, USA). Alternatively, we used sequences that were missing the miR-145-3p target

(262) Regulation of spindle and kinetochore-associated protein 1 by antitumor miR-10a-5p in renal cell carcinomaCancer scienceOctober 1, 2017Arai T, Okato A, Kojima S, Idichi T, Koshizuka K, Kurozumi A, Kato M, Yamazaki K, Ishida Y, Naya Y, et al.inserted between the XhoI‐PmeI restriction sites in the 3′‐UTR of the hRluc gene in the psiCHECK‐2 vector (C8021; Promega, Madison, WI, USA). The procedures were described previously.22, 25, 26, 27, 28 Immunohistochemistry Tissue

(263) The microRNA expression signature of pancreatic ductal adenocarcinoma by RNA sequencing: anti-tumour functions of the microRNA-216 clusterOncotargetSeptember 19, 2017Yonemori K, Seki N, Idichi T, Kurahara H, Osako Y, Koshizuka K, Arai T, Okato A, Kita Y, Arigami T, et al.sequences or lacking a miR-216b-3p target site were inserted in the psiCHECK-2 vector (product ID: C8021; Promega, Madison, WI, USA). The procedure was described previously [20, 28, 51, 52]. Identification

(264) The expression and function of miR-424 in infantile skin hemangioma and its mechanismScientific reportsSeptember 19, 2017Yang L, Dai J, Li F, Cheng H, Yan D, Ruan Q.GAGTCAGGTCAATTTCACTGTCTTT (Biossci, China). Then, the amplified products were inserted into a psiCHECK™-2 Vector (Promega, C8021, USA) at an XhoI site to construct a psiCHECK™-2-FGFR1-3’UTR plasmid. The linker-adapter primers were

(265) miR-361-5p modulates metabolism and autophagy via the Sp1-mediated regulation of PKM2 in prostate cancerOncology reportsSeptember 1, 2017Ling Z, Liu D, Zhang G, Liang Q, Xiang P, Xu Y, Han C, Tao T.restriction sites that were downstream of the open reading frame of luciferase in the psiCHECK-2™ Vector (c8021; Promega, Madison, WI, USA). Deleting the binding site for miR-361-5p generated the mutant reporter.

(266) Regulation of actin-binding protein ANLN by antitumor miR-217 inhibits cancer cell aggressiveness in pancreatic ductal adenocarcinomaOncotargetAugust 8, 2017Tetsuya Idichi et al.inserted between the XhoI–PmeI restriction sites in the 3′-UTR of the hRluc gene in the psiCHECK-2 vector (C8021; Promega, Madison, WI, USA). Alternatively, we used sequences that were missing the miR-217 target sites

(267) miR-99 regulates normal and malignant hematopoietic stem cell self-renewalJournal of Experimental MedicineAugust 7, 2017Mona Khalaj, Carolien M. Woolthuis, Wenhuo Hu, Benjamin H. Durham, S. Haihua Chu, Sarah Qamar, Scott A. Armstrong, Christopher Y. Parkproducts were then cloned in the PsiCHECK-2 vector for measurement of luciferase activity (catalog no. C8021; Promega). Cell cycle analysis BM cells were sorted for the indicated cell populations and subsequently

(268) MicroRNA-143 and -145 modulate the phenotype of synovial fibroblasts in rheumatoid arthritisExperimental & molecular medicineAugust 4, 2017Hong BK, You S, Yoo SA, Park D, Hwang D, Cho CS, Kim WU.NotI sites (Supplementary Table 2). The insert DNA and psiCHECK-2 Dual luciferase reporter vector (C8021, Promega, Madison, MA) were digested by NotI (R0189, NEB, Ipswich, MA, USA) and then ligated. To mutate

(269) Regulation of ITGA 3 by the anti‐tumor miR‐199 family inhibits cancer cell migration and invasion in head and neck cancerCancer ScienceAugust 1, 2017Keiichi Koshizuka, Toyoyuki Hanazawa, Naoko Kikkawa, Takayuki Arai, Atsushi Okato, Akira Kurozumi, Mayuko Kato, Koji Katada, Yoshitaka Okamoto, Naohiko Sekiin miR‐199a‐5p, miR‐199a/b‐3p and miR‐199b‐5p target sites were inserted in the psiCHECK‐2 vector (C8021; Promega, Madison, WI, USA). The vectors were provided by Dr H. Yoshino from Kagoshima University.24

(270) Dual strands of pre-miR‑150 (miR‑150‑5p and miR‑150‑3p) act as antitumor miRNAs targeting SPOCK1 in naïve and castration-resistant prostate cancerInternational journal of oncologyJuly 1, 2017Okato A, Arai T, Kojima S, Koshizuka K, Osako Y, Idichi T, Kurozumi A, Goto Y, Kato M, Naya Y, et al.or those with a deleted miR‑150‑5p or miR‑150‑3p target site were inserted in the psiCHECK-2 vector (C8021; Promega, Madison, WI, USA). Alternatively, we used sequences that were missing the miR‑150‑5p target

(271) Proteasome beta-4 subunit contributes to the development of melanoma and is regulated by miR-148bTumour biology : the journal of the International Society for Oncodevelopmental Biology and MedicineJune 1, 2017Zhang X, Lin D, Lin Y, Chen H, Zou M, Zhong S, Yi X, Han S.Amherst, NY, USA). After digestion with XhoI and NotI, the fragment was inserted into psiCHECK™-2 Vector (C8021; Promega, Madison, WI, USA). Cells were co-transfected with 0.5 µg psiCHECK™-2 vector harboring PSMB4

(272) Thymic Epithelial Tumors phenotype relies on miR-145-5p epigenetic regulationMolecular cancerMay 10, 2017Bellissimo T, Ganci F, Gallo E, Sacconi A, Tito C, De Angelis L, Pulito C, Masciarelli S, Diso D, Anile M, et al.inserted between the XhoI–PmeI restriction sites in the 3’UTR of the hRluc gene in the psiCHECK-2 vector (C8021; Promega, Madison, WI, USA) [50]; while for CDH2 the 3’-UTR sequence of CDH2 carrying a putative miR-145-5p

(273) Regulation of ITGA3 by the dual-stranded microRNA-199 family as a potential prognostic marker in bladder cancerBritish journal of cancerApril 11, 2017Sakaguchi T, Yoshino H, Yonemori M, Miyamoto K, Sugita S, Matsushita R, Itesako T, Tatarano S, Nakagawa M, Enokida H.between the XhoI and PmeI restriction sites in the 30 -UTR of the hRluc gene in the psiCHECK-2 vector (C8021; Promega, Madison, WI, USA). T24 and BOY cells were transfected with 50 ng vector and 10 nM miR199a-5p

(274) Hoxa9 and Meis1 Cooperatively Induce Addiction to Syk Signaling by Suppressing miR-146a in Acute Myeloid LeukemiaCancer cellApril 10, 2017Mohr S, Doebele C, Comoglio F, Berg T, Beck J, Bohnenberger H, Alexe G, Corso J, Ströbel P, Wachter A, et al.DNA,Recombinant DNA ,, pMD2.G,addgene,Cat# 12259 psPAX2,addgene,Cat# 12260 psiCHECK™-2 Vector,Promega,Cat# C8021 LT3-GEPIR,"Fellmann et al., 2013",N/A MSCV-Hoxa9-PGK-neo,"Kroon et al., 1998",N/A MSCV-Meis1-IRES-YFP,"Pineault

(275) microRNA-210-3p depletion by CRISPR/Cas9 promoted tumorigenesis through revival of TWIST1 in renal cell carcinomaOncotargetMarch 28, 2017Yoshino H, Yonemori M, Miyamoto K, Tatarano S, Kofuji S, Nohata N, Nakagawa M, Enokida H.between the XhoI and PmeI restriction sites in the 3′-UTR of the hRluc gene in the psi-CHECK-2 vector (C8021, Promega; Madison, WI, USA). A498 and Caki2 cells were transfected with 5ng of the vector and 10nM miR-210-3p

(276) Interleukin-9 Promotes Pancreatic Cancer Cells Proliferation and Migration via the miR-200a/Beta-Catenin AxisBioMed Research InternationalMarch 2, 2017Bangli Hu, Huang Qiu-lan, Rong-e Lei, Cheng Shi, Hai-xing Jiang, Shan-yu Qinsequences containing the predicted miR200a binding sites were cloned into psiCHECK2 control vector (C8021, Promega, USA) to generate the plasmid psiCHECK2-WT-𝛽-catenin 3󸀠 -UTR. The psiCHECK2MUT-𝛽-catenin 3󸀠

(277) Transcription Factor Trps1 Promotes Tubular Cell Proliferation after Ischemia-Reperfusion Injury through cAMP-Specific 3',5'-Cyclic Phosphodiesterase 4D and AKTJournal of the American Society of Nephrology : JASNFebruary 1, 2017Ju-Rong Y, Ke-Hong C, Kun H, Bi-Qiong F, Li-Rong L, Jian-Guo Z, Kai-Long L, Ya-Ni H.(positions 23000 to +10) was cloned into the psiCHECKTM-2 Dual Luciferase Reporter Plasmid (catalog no. C8021; Promega, Madison, WI) to produce psiCHECK-Pde4d using the PCR method. Using rat genomic DNA as a template,

(278) Systematic characterization of artificial small RNA-mediated inhibition ofEscherichia coligrowthRNA BiologyFebruary 1, 2017Emiko Noro, Masaru Mori, Gakuto Makino, Yuki Takai, Sumiko Ohnuma, Asako Sato, Masaru Tomita, Kenji Nakahigashi, Akio Kanaisynthetic Renilla luciferase gene (hRluc) was first amplified from the psiCHECK-2 plasmid (Promega, #C8021) with tagged PCR primers, and sub-cloned into the HindIII-XhoI sites in the pET-23b vector (Novagen,

(279) ZFP36L2promotes cancer cell aggressiveness and is regulated by antitumormicroRNA-375in pancreatic ductal adenocarcinomaCancer ScienceJanuary 29, 2017Keiichi Yonemori, Naohiko Seki, Hiroshi Kurahara, Yusaku Osako, Tetsuya Idichi, Takayuki Arai, Keiichi Koshizuka, Yoshiaki Kita, Kosei Maemura, Shoji NatsugoeXhoI and PmeI restriction sites in the 3′‐UTR of the hRluc gene in the psiCHECK‐2 vector (product ID: C8021; Promega, Madison, WI, USA). PANC‐1 and SW 1990 cell lines were transfected with 50 ng of the vector

(280) Human microRNA-299-3p decreases invasive behavior of cancer cells by downregulation of Oct4 expression and causes apoptosisPloS oneJanuary 1, 2017Göhring AR, Reuter S, Clement JH, Cheng X, Theobald J, Wölfl S, Mrowka R.was cloned previously by using parts of pcDNA5/FRT (Invitrogen) and psiCHECK-2 (Promega, catalog-no. C8021). Renilla luciferase was coupled with the 3’UTR serving as reporter gene, and Firefly luciferase served

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