psiCHECK-2 Vector from Promega

Product Specs
ItempsiCHECK-2 Vector
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberC8021
Hostmammalian cell line of choice
PromoterSV40 early enhancer/promoter
ResistanceAmp
Quantity20microg
Molecular Weight~78kDa
Nucleic Acid TypeExpression Vectors
Expression System (standardized)Mammalian
Selectable Marker (standardized)Ampicillin
Vector NamepsiCHECK-2

Specifications/Features

Vector Size:
~6.3 kb

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(221) Potential tumor‑suppressive role of microRNA‑99a‑3p in sunitinib‑resistant renal cell carcinoma cells through the regulation of RRM2International journal of oncologyMay 1, 2019Osako Y, Yoshino H, Sakaguchi T, Sugita S, Yonemori M, Nakagawa M, Enokida H.between the XhoI and PmeI restriction sites in the 3'‑UTR of the hRluc gene in a psiCHECK‑2 vector (C8021; Promega Corporation, Madison, WI, USA). ACHN, 786‑o, and SU‑R‑786‑o cells were transfected with 50

(222) Molecular Pathogenesis of Gene Regulation by the miR-150 Duplex: miR-150-3p Regulates TNS4 in Lung AdenocarcinomaCancers (Basel)April 30, 2019Shunsuke Misono et al.Dual Luciferase Reporter Assay The following two sequences were cloned into the psiCHECK-2 vector (C8021; Promega, Madison, WI, USA): the wild-type sequence of the 3’ untranslated regions (UTRs) of TNS4, or

(223) miR‑1271 enhances the sensitivity of colorectal cancer cells to cisplatinExperimental and Therapeutic MedicineApril 18, 2019Huixiang Yao, Qun Sun, Jinshui Zhu3′-UTR of mTOR were inserted downstream of the luciferase in the psiCHECK-2 reporter plasmid (cat. no. C8021; Promega Corporation, Madison, WI, USA). A deletion mutant lacking the seed sequence of the miR-1271

(224) Tumor‑suppressive microRNA‑223 targets WDR62 directly in bladder cancerInternational Journal of OncologyMarch 22, 2019Satoshi Sugita, Hirofumi Yoshino, Masaya Yonemori, Kazutaka Miyamoto, Ryosuke Matsushita, Takashi Sakaguchi, Toshihiko Itesako, Shuichi Tatarano, Masayuki Nakagawa, Hideki Enokidathe XhoI and PmeI restriction sites within the 3'‑UTR of the hRluc gene in the psiCHECK‑2 vector (P/N C8021; Promega, Madison, WI, USA). The T24 and BOY cells were transfected with 50 ng vector and 10 nM miR‑223

(225) Gene Regulation by Antitumor miR-204-5p in Pancreatic Ductal Adenocarcinoma: The Clinical Significance of Direct RACGAP1 RegulationCancers (Basel)March 7, 2019Muhammad Khalid et al.and Dual Luciferase Reporter Assay The following 2 sequences were cloned into the psiCHECk-2 vector (C8021; Promega Corporation, Madison, WI, USA): the wild-type sequence of the 3′-untranslated regions (UTRs)

(226) Communication of prostate cancer cells with bone cells via extracellular vesicle RNA; a potential mechanism of metastasisOncogeneMarch 1, 2019C. Probert, T. Dottorini, A. Speakman, S. Hunt, T. Nafee, A. Fazeli, S. Wood, J. E. Brown, V. Jamescloning the miR21 seed sequences into the 3’-UTR of the psiCHECK2 dual luciferase reporter plasmid (#C8021, Promega, Southampton, UK). hOB cells were seeded into a 96-well plate at a density of 8 × 103 cells

(227) Mammalian Pum1 and Pum2 Control Body Size via Translational Regulation of the Cell Cycle Inhibitor Cdkn1bCell reportsFebruary 26, 2019Lin K, Qiang W, Zhu M, Ding Y, Shi Q, Chen X, Zsiros E, Wang K, Yang X, Kurita T, et al.paper,N/A V14–6 × His-SUMO,Laboratory of Ming Lei,N/A pUC57,Genewiz,N/A psiCHECK-2 Vector,Promega,Cat#C8021 "Chemicals, Reptides, and Recombinant Proteins","Chemicals, Reptides, and Recombinant Proteins","Chemicals,

(228) Regulation of KIF2A by Antitumor miR-451a Inhibits Cancer Cell Aggressiveness Features in Lung Squamous Cell CarcinomaCancers (Basel)February 22, 2019Akifumi Uchida, Naohiko Seki, Keiko Mizuno, Yasutaka Yamada, Shunsuke Misono, Hiroki Sanada, Naoko Kikkawa, Tomohiro Kumamoto, Takayuki Suetsugu, Hiromasa Inoue-UTR, either containing or lacking the miR-451a target site, were cloned into the psiCHECK-2 vector (C8021; Promega, Madison, WI, USA). After cotransfecting miRNA and the constructed vector into EBC-1 and SK-MES-1

(229) Pirin: a potential novel therapeutic target for castration‐resistant prostate cancer regulated by miR‐455‐5pMolecular OncologyFebruary 1, 2019Takayuki Arai, Satoko Kojima, Yasutaka Yamada, Sho Sugawara, Mayuko Kato, Kazuto Yamazaki, Yukio Naya, Tomohiko Ichikawa, Naohiko Sekia sequence having a mutation in the miR‐455‐5p target site was inserted into the psiCHECK‐2 vector (C8021; Promega, Madison, WI, USA). The assay procedure was reported previously (Arai et al., 2017, 2018b;

(230) The tumor-suppressive function of miR-1296-5p by targeting EGFR and CDK6 in gastric cancerBioscience reportsJanuary 31, 2019Jia Y, Zhao LM, Bai HY, Zhang C, Dai SL, Lv HL, Shan BE.sites, were synthesized and inserted between the XhoI-PmeI restriction sites in a psiCHECK-2 vector (C8021; Promega, Madison, WI). Primer sequences for the full-length 3 -UTR of CDK6 mRNA (5 -GCG ATC GCT CGA

(231) Involvement of dual-strand of themiR-144duplex and their targets in the pathogenesis of lung squamous cell carcinomaCancer ScienceJanuary 1, 2019Akifumi Uchida, Naohiko Seki, Keiko Mizuno, Shunsuke Misono, Yasutaka Yamada, Naoko Kikkawa, Hiroki Sanada, Tomohiro Kumamoto, Takayuki Suetsugu, Hiromasa Inouedeletion‐type sequences targeted by miR‐144‐5p and miR‐144‐3p were inserted into the psiCHECK‐2 vector (C8021; Promega, Madison, WI, USA). After cotransfecting miRNA and the constructed vector into EBC‐1 and SK‐MES‐1

(232) Identification of the X-linked germ cell specific miRNAs (XmiRs) and their functionsPloS oneJanuary 1, 2019Ota H, Ito-Matsuoka Y, Matsui Y.cloned into an XhoI-NotI site downstream of a luciferase reporter gene in psiCHECK2 vector (Promega C8021). 1–927 or 927–2263 region of Fzd4 3’UTR were amplified from a cloned WT Fzd4 3’UTR. For Fzd4 3’UTR

(233) Oncogenic Activation of Nrf2, Though as a Master Antioxidant Transcription Factor, Liberated by Specific Knockout of the Full-Length Nrf1α that Acts as a Dominant Tumor RepressorCancersDecember 17, 2018Qiu L, Wang M, Hu S, Ru X, Ren Y, Zhang Z, Yu S, Zhang Y.[97],N/A pcDNA3.1,invitrogen,V79020 pGL3-Basic,Promega,VQP0121 pGL3-promoter,Promega,VQP0124 pRL-TK,Promega,VQP0126 psiCHECK2,Promega,C8021 Software and Algorithms,Software and Algorithms,Software and Algorithms Canvas X,"Canvas GFX, Inc.",https://www.canvasgfx.com/ Chromas

(234) Molecular pathogenesis of esophageal squamous cell carcinoma: Identification of the antitumor effects of miR‑145‑3p on gene regulationInternational Journal of OncologyDecember 6, 2018Masataka Shimonosono et al.(32). Luciferase reporter assays. The following sequences were inserted into the psiCHECk‑2 vector (C8021; Promega Corporation, Madison, WI, USA): The wild‑type sequences of the 3'‑untranslated regions (UTRs)

(235) Loss of microRNA-128 promotes cardiomyocyte proliferation and heart regenerationNature CommunicationsDecember 1, 2018Wei Huang et al.regions (3′UTR) was amplified by PCR and cloned into luciferase reporter vector-psiCHECK2 (Promega, C8021). The reporter vector containing mutant 3′UTR was generated using a Site-Directed Mutagenesis Kit (New

(236) RNAi Modulation of Placental sFLT1 for the Treatment of Preeclampsia.Nature BiotechnologyDecember 1, 2018Anton A. Turanov et al.human sFLT1 i13 and e15a (435 and 567 bp) were cloned into psiCheck-2 vector (Promega, Madison, WI; #C8021) and DualGlo luciferase assay system was used (Promega, Madison, WI; #E2920) according to manufacturer’s

(237) Functional features defining the efficacy of cholesterol-conjugated, self-deliverable, chemically modified siRNAsNucleic Acids ResearchNovember 16, 2018Taisia Shmushkovich, Kathryn R Monopoli, Diana Homsy, Dmitriy Leyfer, Monica Betancur-Boissel, Anastasia Khvorova, Alexey D Wolfsongene by inserting gene fragments or 50-base target site fusions into a psiCheck-2 vector (Promega, C8021) containing independent expression cassettes for two luciferase genes—Renilla luciferase (RLuc) for

(238) miR-186 promotes tumor growth in cutaneous squamous cell carcinoma by inhibiting apoptotic protease activating factor-1Experimental and therapeutic medicineNovember 1, 2018Tian J, Shen R, Yan Y, Deng L.with a mutation in the binding site, were synthesized and cloned into the psi-CHECK2 vector (cat. no. C8021; Promega Corporation, Madison, WI, USA). The 293-T cells were seeded in 96-well plates at a density

(239) MicroRNA-132 provides neuroprotection for tauopathies via multiple signaling pathwaysActa NeuropathologicaOctober 1, 2018Rachid El Fatimy et al.luciferase reporter assay Full-length 3′ UTR sequences of Gsk3β, Calpain2, and Rbfox1 were cloned into psiCHECK2 plasmid (Promega, C8021) downstream of renilla luciferase, using XhoI and NotI. Mutations in the miR-132 binding sites were

(240) MicroRNA-383 acts as a tumor suppressor in colorectal cancer by modulating CREPT/RPRD1B expressionMolecular carcinogenesisOctober 1, 2018Li J, Smith AR, Marquez RT, Li J, Li K, Lan L, Wu X, Zhao L, Ren F, Wang Y, et al.using primers (Supplementary Table S2) and cloned into a psiCHECK2.0 Dual-Luciferase Reporter system (C8021, Promega, Madison, WI). To generate sensor vectors with mutations (psiCHECK2.0-CREPT-3′-MUT) or a deletion

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