psiCHECK-2 Vector from Promega

Product Specs
ItempsiCHECK-2 Vector
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberC8021
Hostmammalian cell line of choice
PromoterSV40 early enhancer/promoter
ResistanceAmp
Quantity20microg
Molecular Weight~78kDa
Nucleic Acid TypeExpression Vectors
Expression System (standardized)Mammalian
Selectable Marker (standardized)Ampicillin
Vector NamepsiCHECK-2

Specifications/Features

Vector Size:
~6.3 kb

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(241) miR‑671‑5p inhibits gastric cancer cell proliferation and promotes cell apoptosis by targeting URGCPExperimental and Therapeutic MedicineOctober 1, 2018Tiefeng Qiu, Keping Wang, Xianwen Li, Jianhua JinWild‑type and mutant 3'UTR of URGCP were amplified and then cloned into the psiCHECK‑2 reporter (cat. no. C8021; Promega Corporation, Madison, WI, USA). miR‑671‑5p and miR‑671‑5p‑URGCP‑wild type (WT) 3'UTR or miR‑671‑5p‑URGCP‑mutant

(242) MicroRNA-125b Promotes Hepatic Stellate Cell Activation and Liver Fibrosis by Activating RhoA SignalingMolecular therapy. Nucleic acidsSeptember 7, 2018You K, Li SY, Gong J, Fang JH, Zhang C, Zhang M, Yuan Y, Yang J, Zhuang SM.Sequences of all RNA oligoes used in this study are listed in Table S2. For vectors, a psiCHECK-2 (cat. C8021; Promega, Madison, WI, USA) vector was used to construct luciferase reporter plasmids, including psiCHECK2-Stard13-3′UTR-WT

(243) Regulation of antitumor miR‐144‐5p targets oncogenes: Direct regulation of syndecan‐3 and its clinical significanceCancer ScienceSeptember 1, 2018Yasutaka Yamada, Takayuki Arai, Satoko Kojima, Sho Sugawara, Mayuko Kato, Atsushi Okato, Kazuto Yamazaki, Yukio Naya, Tomohiko Ichikawa, Naohiko Sekiinserted between the SgfI‐PmeI restriction sites in the 3′‐UTR of the hRluc gene in the psiCHECK‐2 vector (C8021; Promega, Madison, WI, USA). We used sequences that were missing the miR‐144‐5p target sites (position

(244) miR-1266-5p and miR-185-5p Promote Cell Apoptosis in Human Prostate Cancer Cell LinesAsian Pac. J. Cancer PrevAugust 24, 2018Shiva Ostadrahimi, Manuchehr Abedi Abedi Valugerdi, Moustapha Hassan, Ghazal Haddad, Shima Fayaz, Monireh Parvizhamidi, Reza Mahdian, Pezhman Fard Fard Esfahanisynthesized (Biomatiks) and sub-cloned into the XhoI/NotI poly-cloning site of psiCHECK™-2 Vector (Promega, C8021). The vectors were transformed and propagated in the DH5α E. coli strain (Thermo Fisher Scientific,

(245) Distinctive features of lincRNA gene expression suggest widespread RNA-independent functionsLife science allianceAugust 1, 2018Tuck AC, Natarajan KN, Rice GM, Borawski J, Mohn F, Rankova A, Flemr M, Wenger A, Nutiu R, Teichmann S, et al.recombination reporter,Flemr & Buhler (2015), pBluescript II KS(–),Stratagene, psiCHECK-2,Promega,C8021 Oligonucleotides are listed in Table S5,IDT, Reagents used in this study.

(246) Involvement of anti-tumor miR-124-3p and its targets in the pathogenesis of pancreatic ductal adenocarcinoma: direct regulation of ITGA3 and ITGB1 by miR-124-3pOncotargetJune 22, 2018Tetsuya Idichi et al.the 3′-UTR of ITGA3 and ITGB1 in miR-124-3p target sites were inserted into the psiCHECK-2 vector (C8021; Promega, Madison, WI, USA). The procedure for dual luciferase reporter assays was described previously

(247) Self-Delivering RNAi Targeting PD-1 Improves Tumor-Specific T Cell Functionality for Adoptive Cell Therapy of Malignant MelanomaMolecular TherapyJune 1, 2018Maarten A. Ligtenberg, Yago Pico de Coaña, Taisia Shmushkovich, Yuya Yoshimoto, Iva Truxova, Yuan Yang, Monica Betancur-Boissel, Alexey V. Eliseev, Alexey D. Wolfson, Rolf Kiesslingreporter plasmid was constructed by inserting PDCD1 targeting regions into psiCheck2 plasmid (Promega; C8021) downstream Renilla luciferase sequence. Previously validated MAP4K4 sdRNA sequence was also inserted

(248) Molecular pathogenesis of pancreatic ductal adenocarcinoma: Impact of passenger strand of pre-miR-148aon gene regulationCancer ScienceJune 1, 2018Tetsuya Idichi et al.dual‐luciferase reporter assay The vector used for dual‐luciferase reporter assay was psiCHECK‐2 vector (C8021; Promega, Madison, WI, USA). The cloned sequences are listed in Figure S1. The procedure for dual‐luciferase reporter assay

(249) Anti-tumor roles of both strands of the miR-455 duplex: their targets SKA1 and SKA3 are involved in the pathogenesis of renal cell carcinomaOncotargetJune 1, 2018Yasutaka Yamada, Takayuki Arai, Satoko Kojima, Sho Sugawara, Mayuko Kato, Atsushi Okato, Kazuto Yamazaki, Yukio Naya, Tomohiko Ichikawa, Naohiko Sekiinserted between the SgfI-PmeI restriction sites in the 3′-UTR of the hRluc gene in the psiCHECK-2 vector (C8021; Promega, Madison, WI, USA). The procedures were described previously [29, 42, 43]. Immunohistochemistry Tissue

(250) Regulation of NCAPG by miR-99a-3p (passenger strand) inhibits cancer cell aggressiveness and is involved in CRPCCancer medicineMay 1, 2018Arai T, Okato A, Yamada Y, Sugawara S, Kurozumi A, Kojima S, Yamazaki K, Naya Y, Ichikawa T, Seki N.a sequence having a deletion of the miR‐99a‐3p target site was inserted into the psiCHECK‐2 vector (C8021; Promega, Madison, WI). The procedures were reported previously 11, 24, 25, 26. Immunohistochemistry Tissue

(251) miR-148b-3p functions as a tumor suppressor in GISTs by directly targeting KITCell communication and signaling : CCSApril 16, 2018Wang Y, Li J, Kuang D, Wang X, Zhu Y, Xu S, Chen Y, Cheng H, Zhao Q, Duan Y, et al.cloned into the GV141 vector by GeneChem (Shanghai, China) (called pGV141-KIT). The psiCHECK-2 vector (C8021) was purchased from Promega (Madison, WI, USA). The psiCHECK-2-KIT-wt, psiCHECK-2-KIT-mut-1378, and

(252) Impact of novel oncogenic pathways regulated by antitumor miR-451a in renal cell carcinomaCancer scienceApril 1, 2018Yamada Y, Arai T, Sugawara S, Okato A, Kato M, Kojima S, Yamazaki K, Naya Y, Ichikawa T, Seki N.inserted between the SgfI‐PmeI restriction sites in the 3′‐UTR of the hRluc gene in the psiCHECK‐2 vector (C8021; Promega, Madison, WI, USA). The procedures were covered in earlier reports.20, 21, 22, 23, 24, 25 2.8

(253) miR‑21‑5p confers doxorubicin resistance in gastric cancer cells by targeting PTEN and TIMP3International journal of molecular medicineApril 1, 2018Chen J, Zhou C, Li J, Xiang X, Zhang L, Deng J, Xiong J.were inserted downstream of the stop codon of Firefly luciferase in the psi-Check2 vector (cat. no. C8021; Promega Corp., Madison, WI, USA) as previously described (33). To yield mutant constructs, mutations

(254) miR-126-5p by direct targeting of JNK-interacting protein-2 (JIP-2) plays a key role in Theileria-infected macrophage virulencePLOS PathogensMarch 23, 2018Malak Haidar, Zineb Rchiad, Hifzur Rahman Ansari, Fathia Ben-Rached, Shahin Tajeri, Perle Latre De Late, Gordon Langsley, Arnab Painthe identified miR-126-5p seed sequence of bovine JIP-2 was subcloned into the psiCHECK-2 (Promega, # C8021) and then transfected into Theileria-infected macrophages together with the mimic or inhibitor of miR-126-5p

(255) Inhibition of integrin β1-mediated oncogenic signalling by the antitumor microRNA-29 family in head and neck squamous cell carcinomaOncotargetJanuary 9, 2018Keiichi Koshizuka, Naoko Kikkawa, Toyoyuki Hanazawa, Yasutaka Yamada, Atsushi Okato, Takayuki Arai, Koji Katada, Yoshitaka Okamoto, Naohiko Seki(UTR) of ITGB1 in miR-29a, miR-29b, and miR-29c target sites were inserted in the psiCHECK-2 vector (C8021; Promega, Madison, WI, USA). The procedure for dual luciferase reporter assays was described previously

(256) Downregulation of microRNA-1274a induces cell apoptosis through regulation of BMPR1B in clear cell renal cell carcinomaOncology reportsJanuary 1, 2018Yoshino H, Yonezawa T, Yonemori M, Miyamoto K, Sakaguchi T, Sugita S, Osako Y, Tatarano S, Nakagawa M, Enokida H.between the XhoI and PmeI restriction sites in the 3'-UTR of the hRluc gene in the psi-CHECK-2 vector (C8021; Promega, Madison, WI, USA). ACHN cells were transfected with 50 ng of the vector and 10 nM miR-1274a

(257) miR-1266-5p and miR-185-5p Promote Cell Apoptosis in Human Prostate Cancer Cell LinesAsian Pac. J. Cancer PrevJanuary 1, 2018Shiva Ostadrahimi, Manuchehr Abedi Valugerdi, Moustapha Hassan, Ghazal Haddad, Shima Fayaz, Monireh Parvizhamidi, Reza Mahdian, Pezhman Fard Esfahanisynthesized (Biomatiks) and sub-cloned into the XhoI/NotI poly-cloning site of psiCHECK™-2 Vector (Promega, C8021). The vectors were transformed and propagated in the DH5α E. coli strain (Thermo Fisher Scientific,

(258) The dual-inhibitory effect of miR-338-5p on the multidrug resistance and cell growth of hepatocellular carcinomaSignal transduction and targeted therapyJanuary 1, 2018Zhao Y, Chen J, Wei W, Qi X, Li C, Ren J.assay The wild-type 3′-UTR of ABCB1 or EGFR mRNA was inserted into the psiCHECKTM-2 Vector (Promega, #C8021, USA). Mutants lacking the miR-338-5p binding sites in the 3′-UTR of ABCB1 or EGFR was constructed using

(259) Downregulation of matrix metalloproteinase 14 by the antitumor miRNA, miR-150-5p, inhibits the aggressiveness of lung squamous cell carcinoma cellsInternational Journal of OncologyDecember 21, 2017Takayuki Suetsugu, Keiichi Koshizuka, Naohiko Seki, Keiko Mizuno, Atsushi Okato, Takayuki Arai, Shunsuke Misono, Akifumi Uchida, Tomohiro Kumamoto, Hiromasa Inoue3'-untranslated region (UTR) of MMP14 in miR‑150‑5p target sites were inserted in the psiCHECK-2 vector (C8021; Promega, Madison, WI, USA). We used 3 sequences that were putative miR‑150‑5p target sites of MMP14

(260) A miR-327-FGF10-FGFR2-mediated autocrine signaling mechanism controls white fat browningNature communicationsDecember 12, 2017Fischer C, Seki T, Lim S, Nakamura M, Andersson P, Yang Y, Honek J, Wang Y, Gao Y, Chen F, et al.GmbH). Dual-luciferase reporter assay A psiCHECK−2 vector and a Dual-Luciferase Reporter Assay System (C8021, E1910, Promega) were used for target validation. Two ~700 bp long sequences of the 3′UTR of Fgf10 mRNA

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