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Nice Fluorophore Option for Pan T-cell Marker From Invitrogen
July 23, 2023
PBMC-derived T-cells were activated with CD3/CD28 magnetic beads and IL-2 for 3 days and FACS stained with this antibody according... read more
Di-tert-butyl dicarbonate for Boc Protection
July 23, 2023
The aim was to protect just one side of a diamine to be subsequently able to link it to a second scaffold. read more
ECL Substrate - Femto From Thermo Scientific
July 23, 2023
The WNT pathway is essential for embryonic development and adult tissue homeostasis. De-regulation of this pathway results in ... read more
ECL Substrate - Pico PLUS From Thermo Scientific
July 23, 2023
The WNT pathway is essential for embryonic development and adult tissue homeostasis. De-regulation of this pathway results in ... read more
Nice CD4⁺ CD25⁺ Regulatory T Cell Isolation Kit
July 23, 2023
I used this kit in a project to study the release of extracellular vesicles from T regular cells. This kit worked well to isolate ... read more
Trifluoroacetic Acid From Thermo Scientific For Boc Deprotection
July 23, 2023
We need to deprotect a Boc amine to be able then to link it to a carboxylic acid and form a new peptide bond. read more
Cell Surface Labeling Reagent From Thermo Fisher
July 23, 2023
Hedgehog signaling plays a critical role in embryonic development. It also controls adult tissue homeostasis and deregulation of ... read more
Nice Anti-mouse CD45 Antibody For Flow Cytometry From BioLegend
July 23, 2023
We have used this CD45 antibody in several of our projects. It worked well for staining leukocytes in single cells isolated from ... read more
Easy to Use Fixable Viability Dye
July 23, 2023
1 million PBMC-derived T-cells were resuspended in PBS and stained with 1 µl of this dye at RT for 10-15 minutes. Cells were ... read more
Great Phosphatase Inhibitor Cocktail Solution From GenDEPOT
July 23, 2023
We have been using this phosphatase inhibitor cocktail solution in Western blot to prevent proteins from dephosphorylation for ... read more
Synthesis of Schiff Base
July 23, 2023
We synthesized a Schiff base using an amine and an aldehyde. This product will be then linked to a larger scaffold exploiting the ... read more
Great T-Cell Antibody for Flow Cytometry From BioLegend
July 22, 2023
CD8+ T-cell infiltration into the brain during viral respiratory infection in mice is assessed by flow cytometry. read more
Great Antibody for Immunofluorescence From Millipore Sigma
July 22, 2023
Blood vessels in the brain of mice are identified by staining for basement membrane glycoprotein laminin. Regions, where the ... read more
Excellent MHC-II Antibody From BD Biosciences
July 22, 2023
The staining is extremely bright. I'd recommend using much less of this antibody compared to others, especially when running flow ... read more
Ideal Transfection Reagent From Thermo Fisher
July 22, 2023
I am interested in understanding the molecular mechanism of WNT signal transduction. The goal of this experiment is to determine ... read more

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Efficient Myelin Removal from Adult Mouse Brain for Subsequent Astrocyte ...

Ksenia Novototskaya

We use Myelin Isolation Beads during the preparation of single-cell suspensions from adult mouse brain tissue for subsequent immunomagnetic isolation of ... read more

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